##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146616_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4921287
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27636408931241	31.0	31.0	34.0	28.0	34.0
2	31.45990002208772	33.0	31.0	34.0	28.0	34.0
3	31.480450337482857	33.0	31.0	34.0	28.0	34.0
4	34.98996725856468	37.0	35.0	37.0	32.0	37.0
5	35.04971992082559	37.0	35.0	37.0	32.0	37.0
6	35.05396169741777	37.0	35.0	37.0	32.0	37.0
7	35.00999514964277	37.0	35.0	37.0	32.0	37.0
8	35.00007498038623	37.0	35.0	37.0	32.0	37.0
9	36.58960613351751	39.0	37.0	39.0	32.0	39.0
10-11	36.58716520698752	39.0	37.0	39.0	32.0	39.0
12-13	36.54493062485484	39.0	37.0	39.0	32.0	39.0
14-15	37.877592995490815	40.0	38.0	41.0	32.0	41.0
16-17	37.88858229564746	40.0	38.0	41.0	32.0	41.0
18-19	37.89404397670772	40.0	38.0	41.0	32.0	41.0
20-21	37.99646312031792	40.0	38.0	41.0	32.0	41.0
22-23	37.994776976022735	40.0	38.0	41.0	32.0	41.0
24-25	38.00764992978463	40.0	38.0	41.0	32.0	41.0
26-27	38.064220497605604	40.0	38.0	41.0	32.0	41.0
28-29	38.04233740076529	40.0	38.0	41.0	32.0	41.0
30-31	38.00635717851854	40.0	38.0	41.0	32.0	41.0
32-33	37.98016016948412	40.0	38.0	41.0	32.0	41.0
34-35	37.93387735769119	40.0	38.0	41.0	32.0	41.0
36-37	37.86046942598552	40.0	38.0	41.0	31.0	41.0
38-39	37.803003868703456	40.0	38.0	41.0	31.0	41.0
40-41	37.74802434810243	40.0	38.0	41.0	31.0	41.0
42-43	37.59331136347057	40.0	37.0	41.0	31.0	41.0
44-45	37.46377289111568	40.0	37.0	41.0	30.5	41.0
46-47	37.35663191356245	40.0	37.0	41.0	30.0	41.0
48-49	37.248730565805246	40.0	36.5	41.0	30.0	41.0
50-51	37.11629772862261	40.0	36.0	41.0	30.0	41.0
52-53	36.990705276892	40.0	36.0	41.0	30.0	41.0
54-55	36.874462411966626	39.0	35.0	41.0	30.0	41.0
56-57	36.69289862590822	39.0	35.0	41.0	30.0	41.0
58-59	36.498970086483475	39.0	35.0	41.0	30.0	41.0
60-61	36.28810807010443	38.5	35.0	40.5	29.5	41.0
62-63	36.060023526366166	38.0	35.0	40.0	29.0	41.0
64-65	35.77157784132484	37.5	35.0	40.0	29.0	41.0
66-67	35.544865906011985	37.0	34.5	40.0	29.0	41.0
68-69	35.29156234944233	36.5	34.0	39.5	29.0	41.0
70-71	35.02090510063729	36.0	34.0	39.0	29.0	41.0
72-73	34.68776125025832	35.5	34.0	39.0	28.0	41.0
74-75	34.3613563890909	35.0	34.0	38.5	28.0	40.0
76-77	34.092163391405535	35.0	34.0	37.0	28.0	39.5
78-79	33.78172122454959	35.0	34.0	37.0	27.5	39.0
80-81	33.537114071989706	35.0	33.5	36.5	27.0	39.0
82-83	33.26463910761555	35.0	33.0	36.0	27.0	37.5
84-85	33.058442537490706	35.0	33.0	36.0	27.0	37.0
86-87	32.848311833875975	35.0	33.0	35.0	26.5	37.0
88-89	32.66957423942152	35.0	33.0	35.0	26.0	36.0
90-91	32.52187547688237	35.0	33.0	35.0	26.0	36.0
92-93	32.37309620430591	35.0	33.0	35.0	25.5	36.0
94-95	32.25599451931984	35.0	33.0	35.0	25.0	36.0
96-97	32.161941175143824	35.0	33.0	35.0	25.0	35.0
98-99	32.05680892010565	35.0	33.0	35.0	25.0	35.0
100	30.909607182023727	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29508135213198017
1101	2	0.3515141670633213
1101	3	0.3109929523413868
1101	4	0.3148748739947891
1101	5	0.2848877382533672
1101	6	0.35775974374816144
1101	7	0.439299129512321
1101	8	0.409706091858439
1101	9	0.5423722924687482
1101	10-11	0.5084106318355381
1101	12-13	0.5034347229199838
1101	14-15	0.6209168108592351
1101	16-17	0.6199363120758008
1101	18-19	0.5914010187289875
1101	20-21	0.5902021089573282
1101	22-23	0.6205476929582474
1101	24-25	0.6391462103340544
1101	26-27	0.592083701952788
1101	28-29	0.5650784476156829
1101	30-31	0.6329212309135528
1101	32-33	0.6055149224450886
1101	34-35	0.5440960867027087
1101	36-37	0.5805872889190695
1101	38-39	0.6068798656717647
1101	40-41	0.5880260645761197
1101	42-43	0.5926669868954519
1101	44-45	0.5220270077564564
1101	46-47	0.5136600765403827
1101	48-49	0.48413042330574285
1101	50-51	0.4974810806034995
1101	52-53	0.47549216718579856
1101	54-55	0.4814821849458397
1101	56-57	0.5009941485114879
1101	58-59	0.5125606338792181
1101	60-61	0.508064161079389
1101	62-63	0.48828766779544
1101	64-65	0.5686199622718249
1101	66-67	0.5048224690907901
1101	68-69	0.5515615062751991
1101	70-71	0.4610015478977374
1101	72-73	0.4489555531938052
1101	74-75	0.4837192659751466
1101	76-77	0.4696096999504533
1101	78-79	0.4568177923432444
1101	80-81	0.354176440926679
1101	82-83	0.3897982716294379
1101	84-85	0.3225639683646193
1101	86-87	0.3851316843191839
1101	88-89	0.3378282122216234
1101	90-91	0.378024469094548
1101	92-93	0.4361012875081798
1101	94-95	0.40459082268863256
1101	96-97	0.3930510818383155
1101	98-99	0.44709674633022445
1101	100	0.4632531001263658
1102	1	0.2735050802939831
1102	2	0.26885999097073565
1102	3	0.21798350245338582
1102	4	0.1530834228973319
1102	5	0.19761545553765814
1102	6	0.24907056714529574
1102	7	0.3117181775142086
1102	8	0.2867044142676889
1102	9	0.3820202677004261
1102	10-11	0.3758832312191771
1102	12-13	0.277134668145095
1102	14-15	0.43702209670433945
1102	16-17	0.35113776326887347
1102	18-19	0.3539089925049481
1102	20-21	0.38040669716900055
1102	22-23	0.40971288520405125
1102	24-25	0.4403550240371814
1102	26-27	0.40377789688777455
1102	28-29	0.4380812228507196
1102	30-31	0.4691366529231473
1102	32-33	0.4632593200198514
1102	34-35	0.4244320282990657
1102	36-37	0.44956729540151485
1102	38-39	0.4523205732688851
1102	40-41	0.42934331285115945
1102	42-43	0.4341652976940722
1102	44-45	0.40626085496943887
1102	46-47	0.3897073755451217
1102	48-49	0.38811423300369086
1102	50-51	0.4172855789351786
1102	52-53	0.364755201004364
1102	54-55	0.3393975955009054
1102	56-57	0.3597729904567615
1102	58-59	0.35319861108373374
1102	60-61	0.3039399602376065
1102	62-63	0.3295633519400454
1102	64-65	0.40963318747076727
1102	66-67	0.4142217874000309
1102	68-69	0.2912623830832146
1102	70-71	0.28480293009045
1102	72-73	0.2981253487158426
1102	74-75	0.3363826681338651
1102	76-77	0.3057334808579171
1102	78-79	0.2548077519713985
1102	80-81	0.18448865877111587
1102	82-83	0.22079783432795352
1102	84-85	0.26184394493260044
1102	86-87	0.2649832476866294
1102	88-89	0.1930227333277159
1102	90-91	0.2210110879813243
1102	92-93	0.13992422158064244
1102	94-95	0.17182027678768463
1102	96-97	0.18266833278672578
1102	98-99	0.2128807492525837
1102	100	0.23881591845540484
1103	1	0.2403639394420658
1103	2	0.23512940633602497
1103	3	0.27000745931545467
1103	4	0.29715524811667393
1103	5	0.2603405757900532
1103	6	0.29737123432630597
1103	7	0.3196975153272703
1103	8	0.2823669289061712
1103	9	0.463212046705074
1103	10-11	0.382061920769587
1103	12-13	0.35502863445518074
1103	14-15	0.46457633915171215
1103	16-17	0.4306497832087288
1103	18-19	0.35939517508926144
1103	20-21	0.3467716920452375
1103	22-23	0.3679092890252491
1103	24-25	0.46551685031725043
1103	26-27	0.44071049391452277
1103	28-29	0.4481191864514429
1103	30-31	0.447369276324018
1103	32-33	0.4498130549719761
1103	34-35	0.44410313995722106
1103	36-37	0.39049969218191904
1103	38-39	0.4527156972240718
1103	40-41	0.3565844124173765
1103	42-43	0.4487056082395142
1103	44-45	0.338213960512185
1103	46-47	0.35894233552513555
1103	48-49	0.29766506996179487
1103	50-51	0.3637600999127457
1103	52-53	0.37387123195087923
1103	54-55	0.2626881178900575
1103	56-57	0.312535516055803
1103	58-59	0.32802829013830603
1103	60-61	0.24025710423396873
1103	62-63	0.2753692579241189
1103	64-65	0.23022193913007527
1103	66-67	0.24905483782576
1103	68-69	0.2517650013924069
1103	70-71	0.2647660365154323
1103	72-73	0.2729315076573329
1103	74-75	0.32929927929275493
1103	76-77	0.284127038076079
1103	78-79	0.22557035724133812
1103	80-81	0.21339045030649828
1103	82-83	0.20673001775397637
1103	84-85	0.20081496880402483
1103	86-87	0.17195536687019342
1103	88-89	0.18429289008787464
1103	90-91	0.19830950736465525
1103	92-93	0.18349641638184266
1103	94-95	0.21484630295488216
1103	96-97	0.25616282940563195
1103	98-99	0.16437636117109378
1103	100	0.27060695368528087
1104	1	0.1908684544015422
1104	2	0.20264646847814305
1104	3	0.2212218417382381
1104	4	0.1742989829500985
1104	5	0.13610687821513068
1104	6	0.16516897324370206
1104	7	0.18566588704331366
1104	8	0.19549131770575912
1104	9	0.2879437199029482
1104	10-11	0.2817268271790567
1104	12-13	0.27155714070710246
1104	14-15	0.32315440458083344
1104	16-17	0.35612267150960264
1104	18-19	0.3194867041291758
1104	20-21	0.3361471718629616
1104	22-23	0.35151658642636363
1104	24-25	0.39795530768397924
1104	26-27	0.4141844419454799
1104	28-29	0.35440513045369926
1104	30-31	0.3937918908341942
1104	32-33	0.4392192756309683
1104	34-35	0.3677489690578639
1104	36-37	0.4027826574540896
1104	38-39	0.3559409608427657
1104	40-41	0.33586347088294133
1104	42-43	0.4032407058392451
1104	44-45	0.4037679864300614
1104	46-47	0.34670938777968985
1104	48-49	0.3425356349832285
1104	50-51	0.36398146214268934
1104	52-53	0.3328005361427202
1104	54-55	0.27544608713079555
1104	56-57	0.3012508317020419
1104	58-59	0.3069551237937347
1104	60-61	0.28023430595056453
1104	62-63	0.2221536434838285
1104	64-65	0.2944859766258858
1104	66-67	0.23397845436599596
1104	68-69	0.2749046197487104
1104	70-71	0.262496101155719
1104	72-73	0.26779062477646676
1104	74-75	0.23586351621936075
1104	76-77	0.23623572217411493
1104	78-79	0.25143918150980227
1104	80-81	0.26734735569958445
1104	82-83	0.27754823665975437
1104	84-85	0.2607244149758614
1104	86-87	0.22475174861791913
1104	88-89	0.2091734022690801
1104	90-91	0.24720414277902591
1104	92-93	0.21444548703213684
1104	94-95	0.22129829300988035
1104	96-97	0.20893149346849071
1104	98-99	0.24569425148469293
1104	100	0.2672596368991229
1105	1	0.14744864460662654
1105	2	0.14021064806082606
1105	3	0.08200860193626625
1105	4	0.11388616336149937
1105	5	0.05882575731752837
1105	6	0.1935712073986693
1105	7	0.16106528999528535
1105	8	0.17623691917465578
1105	9	0.2435115778930097
1105	10-11	0.24199201755358501
1105	12-13	0.21283660416486327
1105	14-15	0.2626464208348338
1105	16-17	0.22431495301558613
1105	18-19	0.2296632307144435
1105	20-21	0.26941522424792197
1105	22-23	0.25221921152763827
1105	24-25	0.2410348185851845
1105	26-27	0.23351490402615838
1105	28-29	0.2070701273575537
1105	30-31	0.24131860857958998
1105	32-33	0.22104643361456056
1105	34-35	0.19256731303516972
1105	36-37	0.24027289920000072
1105	38-39	0.190732225087352
1105	40-41	0.2132822701940924
1105	42-43	0.2330073703493909
1105	44-45	0.19554991910349884
1105	46-47	0.2006713775701101
1105	48-49	0.23330474252273348
1105	50-51	0.21583955078799733
1105	52-53	0.19122541376020052
1105	54-55	0.17973759321372285
1105	56-57	0.09159704562269155
1105	58-59	0.14356833956590975
1105	60-61	0.17392414288682545
1105	62-63	0.11379195190935576
1105	64-65	0.11961618423716658
1105	66-67	0.13309095386536995
1105	68-69	0.08395636699221143
1105	70-71	0.11029201424178581
1105	72-73	0.16987906059072344
1105	74-75	0.14766849285192762
1105	76-77	0.1644019097852194
1105	78-79	0.15627721143614082
1105	80-81	0.09691793090514267
1105	82-83	0.1476515955940627
1105	84-85	0.24222313492705894
1105	86-87	0.14237446232137074
1105	88-89	0.16312870684675573
1105	90-91	0.14826043719949666
1105	92-93	0.21002720836659705
1105	94-95	0.18381421275036303
1105	96-97	0.2038309226067767
1105	98-99	0.1849304120160653
1105	100	0.23474140660124476
1106	1	0.09493467689296153
1106	2	0.18281212958868664
1106	3	0.12543101838835824
1106	4	0.11723698982511621
1106	5	0.14614838526451024
1106	6	0.0738949978752359
1106	7	0.1125850571120921
1106	8	0.14038537378091576
1106	9	0.1973248374730474
1106	10-11	0.23721517085637345
1106	12-13	0.19963488972972954
1106	14-15	0.2565281699231079
1106	16-17	0.21514994345026395
1106	18-19	0.2685485609075613
1106	20-21	0.3005593476137278
1106	22-23	0.2545119113114467
1106	24-25	0.25386669288670305
1106	26-27	0.2458799904914315
1106	28-29	0.24774774584356152
1106	30-31	0.2493621473493306
1106	32-33	0.25807233857852196
1106	34-35	0.22890482107985832
1106	36-37	0.21146022660409614
1106	38-39	0.20712421328585862
1106	40-41	0.2294231395607227
1106	42-43	0.2990573826008003
1106	44-45	0.2398849403422858
1106	46-47	0.279670390032031
1106	48-49	0.24850195213065263
1106	50-51	0.2080747441887283
1106	52-53	0.2059290349405174
1106	54-55	0.19568825965642844
1106	56-57	0.23754440689325662
1106	58-59	0.21555366436077605
1106	60-61	0.21491070566633397
1106	62-63	0.1976759164864248
1106	64-65	0.2808099413750469
1106	66-67	0.26836233819869904
1106	68-69	0.19714762608620617
1106	70-71	0.19433324039443534
1106	72-73	0.2280778441188076
1106	74-75	0.20536273091543222
1106	76-77	0.2015567004084815
1106	78-79	0.22652834431549707
1106	80-81	0.19204407630181208
1106	82-83	0.19326118512861257
1106	84-85	0.20340435970305037
1106	86-87	0.11399882699885211
1106	88-89	0.15291611624801504
1106	90-91	0.1594005028494081
1106	92-93	0.16761179578899998
1106	94-95	0.16314578934557034
1106	96-97	0.15773927361485818
1106	98-99	0.1772942000925184
1106	100	0.26030501671615625
1107	1	0.17834374320762336
1107	2	0.18777330789264113
1107	3	0.16032935281472405
1107	4	0.22077904527449022
1107	5	0.19677271847547217
1107	6	0.21503670337268943
1107	7	0.3077011394037825
1107	8	0.28126874524941314
1107	9	0.35492069267390747
1107	10-11	0.30952916952759324
1107	12-13	0.3695300726601687
1107	14-15	0.3424671116497038
1107	16-17	0.3338918230275638
1107	18-19	0.3026640619915213
1107	20-21	0.39790691889689356
1107	22-23	0.417334476976869
1107	24-25	0.4403875241725288
1107	26-27	0.3747115077494598
1107	28-29	0.37996391421088305
1107	30-31	0.4083133552182261
1107	32-33	0.4063379735973953
1107	34-35	0.41127783371613447
1107	36-37	0.3895318052537178
1107	38-39	0.3752036748149621
1107	40-41	0.36826710208641344
1107	42-43	0.3299454031467448
1107	44-45	0.24725103488248834
1107	46-47	0.306009873206186
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2216	54-55	-0.36220457827591446
2216	56-57	-0.38210644776570746
2216	58-59	-0.3865037610115323
2216	60-61	-0.3802761693815526
2216	62-63	-0.45437496024845103
2216	64-65	-0.4371047418232905
2216	66-67	-0.3832875477561615
2216	68-69	-0.42821059261851957
2216	70-71	-0.401521101434561
2216	72-73	-0.3386977468442396
2216	74-75	-0.3530175068618888
2216	76-77	-0.4179703479352952
2216	78-79	-0.3719863917661712
2216	80-81	-0.3552099029221196
2216	82-83	-0.3823213758911024
2216	84-85	-0.34668975121851275
2216	86-87	-0.4045690724865594
2216	88-89	-0.3144076656848327
2216	90-91	-0.346935695135258
2216	92-93	-0.2831330602356985
2216	94-95	-0.3760228055310044
2216	96-97	-0.3871300131108555
2216	98-99	-0.3670993837255132
2216	100	-0.42178623092695133
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1547.0
1	369.0
2	283.0
3	263.0
4	250.0
5	276.0
6	460.0
7	1090.0
8	3166.0
9	4696.0
10	5140.0
11	5875.0
12	7568.0
13	9159.0
14	11497.0
15	12508.0
16	13481.0
17	14782.0
18	16300.0
19	18485.0
20	20454.0
21	22322.0
22	25345.0
23	29174.0
24	35067.0
25	43127.0
26	48077.0
27	47380.0
28	50230.0
29	62319.0
30	72944.0
31	83327.0
32	104880.0
33	140050.0
34	203583.0
35	309294.0
36	562545.0
37	1141390.0
38	1355634.0
39	436052.0
40	898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97872329173482	20.26368278007193	13.26133022751376	24.496263700679485
2	14.78530200740483	20.581902796198808	40.809078064252326	23.823717132144033
3	17.54603989208857	26.579087259317287	30.449065617649772	25.42580723094437
4	12.115549928201753	17.669100072377837	40.27986368806473	29.935486311355675
5	13.07128997705414	38.457895069721204	35.76440542606636	12.706409527158295
6	29.41504514789069	39.22459562959225	18.589984911043494	12.770374311473567
7	26.106875817950524	32.92723329092956	23.591569275261513	17.374321615858403
8	25.14759823018389	35.71308722272898	21.088857907276847	18.050456639810285
9	26.08965754389875	15.583545139592847	21.012346818778095	37.314450497730306
10-11	24.312724621603635	26.599631199861307	29.150066677885565	19.937577500649493
12-13	24.768727322121244	24.51472084603891	28.38501781695348	22.331534014886365
14-15	22.371921829920268	25.66647462474038	25.933601351465402	26.02800219387395
16-17	22.090523147337976	28.42059302308201	26.781387116746764	22.707496712833255
18-19	21.96287977158034	28.41030532216588	27.868439061144763	21.75837584510902
20-21	23.32709056487904	27.538081904308733	27.046631650226022	22.08819588058621
22-23	23.26361739419731	27.46125606230347	26.71693158221926	22.558194961279955
24-25	22.8866696391485	27.583875590958705	27.1934907731893	22.335963996703494
26-27	22.70050697836199	27.750504787281688	26.856259336874377	22.692728897481945
28-29	22.448390032161633	27.991550873165583	26.962488467763478	22.59757062690931
30-31	22.897899859903887	27.58326424453702	26.952369049995404	22.566466845563685
32-33	22.818559356082805	27.835200412129137	26.619842500532094	22.72639773125597
34-35	22.982414018264574	27.83312389771732	26.995818711229113	22.18864337278899
36-37	22.938264368480013	27.59088017338092	26.914332969140116	22.55652248899895
38-39	22.915330455744744	27.60313887322526	27.12281988088634	22.358710790143657
40-41	22.861102620093998	27.215265689518382	27.2941355442925	22.629496146095118
42-43	22.690156927159734	27.35426332750012	27.089490948762947	22.8660887965772
44-45	22.64869866455202	27.81023160422261	26.973643199270587	22.567426531954784
46-47	22.70323186442484	27.492100377323037	26.911493109527807	22.893174648724315
48-49	22.941128732578452	27.21856687455673	27.11537440651873	22.724929986346094
50-51	22.71025150992292	27.416962247935594	26.856934937070402	23.015851305071088
52-53	22.512305336487227	27.585513939946072	26.868108170007126	23.034072553559575
54-55	22.53659239987732	27.496579123132857	26.920653554254457	23.046174922735368
56-57	22.87868300690751	27.378184993368293	26.822366492811096	22.920765506913103
58-59	22.592937664039127	27.336323442770556	27.200795229032614	22.86994366415771
60-61	22.68468129465307	27.556827947420608	26.93764206351224	22.820848694414074
62-63	22.737401742290437	27.25984722998968	26.793840423509785	23.2089106042101
64-65	22.329797506105777	27.400972733876067	26.956690743976047	23.312539016042116
66-67	22.732092658061	27.20889763106828	26.806874905922122	23.2521348049486
68-69	22.450810648966662	27.11335416739553	27.07347668209559	23.362358501542214
70-71	22.587461711775163	26.944577028580508	27.046211117180658	23.421750142463672
72-73	22.67942910737158	26.988024126268918	27.201406097795882	23.13114066856362
74-75	22.5482192190155	27.333613296921115	27.13885587106324	22.979311613000142
76-77	22.54295689919075	27.18850590844879	27.009264146664187	23.259273045696276
78-79	22.414223268438345	27.130658138721437	27.119978543166873	23.335140049673342
80-81	22.398527952138103	27.225511780624025	27.214781827507306	23.161178439730566
82-83	22.6698360900509	26.757800302730043	27.664129134440657	22.908234472778403
84-85	22.8213224553626	27.024246594819935	27.073553842232673	23.080877107584794
86-87	22.28870974762697	27.217401969857786	27.29472647486094	23.199161807654303
88-89	22.751925322975445	26.625789869573126	27.214879102612972	23.407405704838457
90-91	22.68506676801632	26.70918972111929	27.31449082732417	23.29125268354022
92-93	22.81973922407503	26.652888842922128	27.33197650041039	23.195395432592445
94-95	22.26172425190044	26.685226145984288	27.555792845702083	23.49725675641319
96-97	22.47084198030773	26.691457004550106	27.39552673586687	23.4421742792753
98-99	22.33240419209973	26.97639487641296	27.16193155870951	23.529269372777794
100	22.899795406046888	26.563196391454802	26.846438020091114	23.6905701824072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1725.0
1	1095.5
2	480.0
3	491.5
4	487.0
5	448.0
6	406.0
7	394.0
8	403.0
9	413.0
10	415.5
11	414.5
12	454.0
13	515.5
14	541.0
15	618.0
16	726.5
17	908.5
18	1238.0
19	1633.0
20	2204.0
21	3245.5
22	4993.0
23	7500.0
24	10574.5
25	14175.5
26	19328.0
27	26179.5
28	33821.5
29	42968.0
30	53153.5
31	64101.0
32	78658.0
33	92055.0
34	102351.5
35	114372.5
36	128080.5
37	145272.5
38	155866.5
39	154530.0
40	151975.5
41	150742.0
42	152318.5
43	160706.5
44	172287.0
45	185950.0
46	195955.0
47	202104.5
48	205751.5
49	202554.5
50	196223.5
51	194614.0
52	190093.5
53	180967.0
54	172091.5
55	155769.0
56	140567.5
57	123937.0
58	105812.0
59	94121.5
60	79252.5
61	61385.5
62	47711.5
63	37049.0
64	26710.0
65	17873.5
66	12406.5
67	9154.5
68	7021.5
69	5212.0
70	4048.0
71	3123.5
72	2372.0
73	1924.0
74	1479.5
75	1011.0
76	693.0
77	512.5
78	382.5
79	244.5
80	146.5
81	93.5
82	69.5
83	62.5
84	59.5
85	53.5
86	42.0
87	38.0
88	31.5
89	23.5
90	24.5
91	21.5
92	15.5
93	14.5
94	21.5
95	21.5
96	17.5
97	13.5
98	7.0
99	7.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10007544774364918
2	0.06392636722873508
3	0.07967428032545144
4	0.08201106743012548
5	0.08249874473892703
6	0.09034222145548512
7	0.08680656096667397
8	0.08924494751068165
9	0.07445206914370164
10-11	0.08106619264432252
12-13	0.07109928764569105
14-15	0.08042611617652048
16-17	0.08246826490712694
18-19	0.08172658899999126
20-21	0.07309063665663068
22-23	0.07737813299651088
24-25	0.08045659600832059
26-27	0.07358847390936557
28-29	0.08315914109459578
30-31	0.08748727721020945
32-33	0.08903158868808098
34-35	0.09146997523208869
36-37	0.09109405730655416
38-39	0.09254692928902541
40-41	0.08790383491147742
42-43	0.0936950029534957
44-45	0.09088069848395348
46-47	0.0869691200696078
48-49	0.09643818781550437
50-51	0.09270948839195926
52-53	0.09799265923730928
54-55	0.08979358448308339
56-57	0.0966007469184382
58-59	0.10093904297798523
60-61	0.09981128920138167
62-63	0.10043104578131695
64-65	0.10241223484832322
66-67	0.1045864628500634
68-69	0.10117272168845265
70-71	0.10406830570946178
72-73	0.10131496090351975
74-75	0.10508430010279832
76-77	0.10204647686672205
78-79	0.10912795778827773
80-81	0.10449502335466312
82-83	0.10883331941421014
84-85	0.10513509982246513
86-87	0.10145720011858687
88-89	0.10096952280978533
90-91	0.10106096230518562
92-93	0.0953104340389008
94-95	0.09473131723469898
96-97	0.0931971657007608
98-99	0.10724836816060515
100	0.24487496868197284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4921287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.809058006362505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.38797107584821	34.913073023997
2	15.525073553359094	16.08678872558052
3	6.7845367677658	10.545013768424328
4	3.5144329052538117	7.283178329910554
5	2.042190274767493	5.290197720272922
6	1.2802341860249846	3.979663632329803
7	0.8545355989759673	3.0990879091093335
8	0.5784036935399589	2.397324040776484
9	0.4125683240257078	1.9237298607932094
>10	1.5821847185465632	12.721274138375085
>50	0.029360153830700085	1.0373029252248593
>100	0.008360136107878205	0.6667365047436091
>500	1.4861195386438908E-4	0.0566294204623638
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2191932720038478E-4
2	0.0	0.0	0.0	0.0	1.2191932720038478E-4
3	0.0	0.0	0.0	0.0	1.2191932720038478E-4
4	0.0	0.0	0.0	0.0	1.2191932720038478E-4
5	0.0	0.0	0.0	0.0	1.6255910293384637E-4
6	0.0	0.0	0.0	0.0	2.0319887866730797E-4
7	0.0	0.0	0.0	0.0	2.0319887866730797E-4
8	0.0	0.0	0.0	0.0	2.0319887866730797E-4
9	0.0	0.0	0.0	0.0	2.0319887866730797E-4
10-11	0.0	0.0	0.0	0.0	2.2351876653403877E-4
12-13	0.0	0.0	0.0	0.0	4.368775891347121E-4
14-15	0.0	1.0159943933365398E-5	0.0	1.0159943933365398E-5	5.791168042018277E-4
16-17	0.0	2.0319887866730796E-5	0.0	2.0319887866730796E-5	6.705562996021162E-4
18-19	0.0	2.0319887866730796E-5	0.0	2.0319887866730796E-5	7.315159632023087E-4
20-21	0.0	2.0319887866730796E-5	0.0	2.0319887866730796E-5	9.042350100695205E-4
22-23	0.0	2.0319887866730796E-5	0.0	1.4223921506711557E-4	9.550347297363474E-4
24-25	0.0	2.0319887866730796E-5	0.0	3.4543809373442354E-4	9.753546176030783E-4
26-27	0.0	2.0319887866730796E-5	0.0	6.4007646780202E-4	0.0010261543372699052
28-29	0.0	2.0319887866730796E-5	0.0	0.0027838246377421194	0.001097273944803463
30-31	0.0	2.0319887866730796E-5	0.0	0.009519867465563379	0.0011175938326701937
32-33	0.0	2.0319887866730796E-5	0.0	0.026740972432617728	0.0011582336084036554
34-35	0.0	2.0319887866730796E-5	0.0	0.054376019931371616	0.0012191932720038478
36-37	0.0	2.0319887866730796E-5	0.0	0.10049200544491715	0.0012293532159372133
38-39	0.0	2.0319887866730796E-5	0.0	0.17028066032320407	0.0012699929916706748
40-41	0.0	2.0319887866730796E-5	0.0	0.2527794050621311	0.00128015293560404
42-43	0.0	2.0319887866730796E-5	0.0	0.3374828576345984	0.0013411125992042324
44-45	0.0	2.0319887866730796E-5	0.0	0.4458996193475406	0.001432552094604521
46-47	0.0	2.0319887866730796E-5	0.0	0.5790355246503608	0.0015138316460714444
48-49	0.0	2.0319887866730796E-5	0.0	0.7229816102982818	0.001554471421804906
50-51	0.0	2.0319887866730796E-5	0.0	0.8760106858226313	0.0016662308050719252
52-53	0.0	2.0319887866730796E-5	0.0	1.0311123899093875	0.0017475103565388484
54-55	0.0	2.0319887866730796E-5	0.0	1.2040346356552667	0.0017881501322723101
56-57	0.0	2.0319887866730796E-5	0.0	1.4103729369979845	0.001808470020139041
58-59	0.0	2.0319887866730796E-5	0.0	1.6489893802170044	0.0018287899080057716
60-61	0.0	2.0319887866730796E-5	0.0	1.9057108435252812	0.0018491097958725025
62-63	0.0	2.0319887866730796E-5	0.0	2.162716785263692	0.001991349010939618
64-65	0.0	2.0319887866730796E-5	0.0	2.4318435401146083	0.0020116688988063487
66-67	0.0	2.0319887866730796E-5	0.0	2.732994438243492	0.0020116688988063487
68-69	0.0	2.0319887866730796E-5	0.0	3.080007323287587	0.0020116688988063487
70-71	0.0	2.0319887866730796E-5	0.0	3.456341806523375	0.0020319887866730793
72-73	0.0	2.0319887866730796E-5	0.0	3.8345660393307686	0.002174228001740195
74-75	0.0	2.0319887866730796E-5	0.0	4.217616245506511	0.0023063072728739453
76-77	0.0	2.0319887866730796E-5	0.0	4.647767951757335	0.002316467216807311
78-79	0.0	2.0319887866730796E-5	0.0	5.119108070714022	0.0023672669364741376
80-81	0.0	4.063977573346159E-5	0.0	5.631159085011705	0.0023977467682742342
82-83	0.0	4.063977573346159E-5	0.0	6.158805613247104	0.0025399859833413496
84-85	0.0	4.063977573346159E-5	0.0	6.6801326563559495	0.0025399859833413496
86-87	0.0	4.063977573346159E-5	0.0	7.222257104696393	0.002631425478741638
88	0.0	4.063977573346159E-5	0.0	7.651352176778148	0.002702545086275196
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5120	0.0	33.797176	1
GTATCAA	7415	0.0	32.405018	1
ATCAACG	7850	0.0	28.267155	3
CAACGCA	7825	0.0	28.236444	5
AACGCAG	8980	0.0	25.381948	6
GTACTAG	2285	0.0	25.311708	1
GTACATG	15980	0.0	25.24719	1
TATCAAC	9520	0.0	24.789999	2
TACATGG	15680	0.0	24.405521	2
TCAACGC	9155	0.0	24.34002	4
ACATGGG	16255	0.0	22.819168	3
ACGCAGA	9710	0.0	22.79544	7
CGCAGAG	10115	0.0	21.929182	8
CTAACGC	1000	0.0	20.68533	3
CATGGGG	12505	0.0	20.601448	4
CTAGTAC	2995	0.0	20.405926	3
GCTATTG	2965	0.0	20.129145	8
TCTAACG	1075	0.0	20.11681	2
GGGCTAT	3275	0.0	19.515837	6
GCATCGT	2405	0.0	19.344898	8
>>END_MODULE