FastQCFastQC Report
Mon 16 Jan 2017
ERR1146616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921287
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116890.23751916927421626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112810.2292286550245901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60450.12283372215438768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89800.054.777411
GTATCAA155700.040.714341
ATCAACG190350.032.460693
CAACGCA192450.032.1386875
TATCAAC201500.031.1558152
AACGCAG207000.030.4236536
TCAACGC205150.030.1876664
ACGCAGA226950.027.2312837
CGCAGAG230850.026.7915948
GCAGAGT255650.023.9168539
GTACATG157950.022.5383661
TACATGG155100.022.4970232
GAGTACT166300.022.42495512-13
ACATGGG153100.022.0221253
AGAGTAC231550.021.71769510-11
CAGAGTA247600.021.69553410-11
CTAACGC9750.021.2208753
TCTAACG10300.020.5450972
GTACTTT187500.019.7516814-15
TGATCGC15750.019.694099
Produced by FastQC (version 0.11.5)