FastQCFastQC Report
Mon 16 Jan 2017
ERR1146615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6176135
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.040.9779051
GTATCAA80250.035.3923871
CAACGCA82850.032.67955
ATCAACG87150.031.2848593
AACGCAG98600.028.5482336
TCAACGC99000.027.8248314
TATCAAC102450.027.5306472
ACGCAGA103100.026.3450187
CGCAGAG107600.025.2864918
GTACATG183500.025.0366651
TACATGG179050.024.4428862
ACATGGG181250.022.9789053
TGATCGC17600.022.693819
CATGGGG143800.021.2156284
GTATAAG30350.020.7617551
GTGATCG19450.020.0530668
GCAGAGT132750.019.9285189
TAACGCC12000.019.1948594
CTAACGC11800.019.121983
GTACTAG29250.018.4879991
Produced by FastQC (version 0.11.5)