##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146615_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6176135
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15680486258801	31.0	31.0	34.0	28.0	34.0
2	31.43919943459785	31.0	31.0	34.0	28.0	34.0
3	31.539141226673316	33.0	31.0	34.0	28.0	34.0
4	35.07322799776883	37.0	35.0	37.0	32.0	37.0
5	35.03911766825045	37.0	35.0	37.0	32.0	37.0
6	35.16273203224994	37.0	35.0	37.0	32.0	37.0
7	35.086397560934145	37.0	35.0	37.0	32.0	37.0
8	35.068105052755485	37.0	35.0	37.0	32.0	37.0
9	36.697595502688976	39.0	37.0	39.0	32.0	39.0
10-11	36.67913007082909	39.0	37.0	39.0	32.0	39.0
12-13	36.622932060260986	39.0	37.0	39.0	32.0	39.0
14-15	37.96343870397911	40.0	38.0	41.0	32.0	41.0
16-17	37.97461567792803	40.0	38.0	41.0	32.0	41.0
18-19	37.989522735497204	40.0	38.0	41.0	32.0	41.0
20-21	38.07944653088056	40.0	38.0	41.0	32.0	41.0
22-23	38.08307080398987	40.0	38.0	41.0	32.0	41.0
24-25	38.09201175168613	40.0	38.0	41.0	32.0	41.0
26-27	38.14238395048036	40.0	38.0	41.0	32.0	41.0
28-29	38.11426247968997	40.0	38.0	41.0	32.0	41.0
30-31	38.07552749413671	40.0	38.0	41.0	32.0	41.0
32-33	38.04123039732778	40.0	38.0	41.0	32.0	41.0
34-35	37.99669008206589	40.0	38.0	41.0	32.0	41.0
36-37	37.931626494563346	40.0	38.0	41.0	32.0	41.0
38-39	37.87900402112324	40.0	38.0	41.0	32.0	41.0
40-41	37.82853572663163	40.0	38.0	41.0	31.5	41.0
42-43	37.686048961041166	40.0	37.5	41.0	31.0	41.0
44-45	37.57518982340898	40.0	37.0	41.0	31.0	41.0
46-47	37.473089480718926	40.0	37.0	41.0	31.0	41.0
48-49	37.373351942598404	40.0	37.0	41.0	31.0	41.0
50-51	37.25090424674978	40.0	36.0	41.0	30.0	41.0
52-53	37.12749591775439	40.0	36.0	41.0	30.0	41.0
54-55	37.01562983969748	39.5	36.0	41.0	30.0	41.0
56-57	36.840243048443725	39.0	35.0	41.0	30.0	41.0
58-59	36.656916016246406	39.0	35.0	41.0	30.0	41.0
60-61	36.44758388539111	39.0	35.0	41.0	30.0	41.0
62-63	36.222623453017135	38.0	35.0	40.0	29.5	41.0
64-65	35.939487316906124	38.0	35.0	40.0	29.0	41.0
66-67	35.70961734158985	37.0	35.0	40.0	29.0	41.0
68-69	35.45636210996035	37.0	34.5	40.0	29.0	41.0
70-71	35.17138104979894	36.0	34.0	39.0	29.0	41.0
72-73	34.8295539200487	36.0	34.0	39.0	28.5	41.0
74-75	34.493133974565	35.0	34.0	38.5	28.0	40.0
76-77	34.21059967115356	35.0	34.0	37.0	28.0	39.5
78-79	33.8880584702245	35.0	34.0	37.0	28.0	39.0
80-81	33.6353550400048	35.0	34.0	36.5	28.0	39.0
82-83	33.35870329906973	35.0	33.5	36.0	27.0	37.5
84-85	33.14439216435522	35.0	33.0	36.0	27.0	37.0
86-87	32.93264986921432	35.0	33.0	35.0	27.0	37.0
88-89	32.74992466971658	35.0	33.0	35.0	26.5	36.0
90-91	32.60115954395427	35.0	33.0	35.0	26.0	36.0
92-93	32.45413871296531	35.0	33.0	35.0	26.0	36.0
94-95	32.33426406644285	35.0	33.0	35.0	26.0	36.0
96-97	32.24299671234518	35.0	33.0	35.0	25.0	35.0
98-99	32.13684334944104	35.0	33.0	35.0	25.0	35.0
100	31.00539172152163	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2942902208467473
1101	2	0.3158944955631675
1101	3	0.33287893581067607
1101	4	0.3307616039926984
1101	5	0.2850235071729088
1101	6	0.3254768359467164
1101	7	0.39575050589817806
1101	8	0.3703006363793335
1101	9	0.4795148770076878
1101	10-11	0.4734753684336468
1101	12-13	0.46832317732823725
1101	14-15	0.5564484980243876
1101	16-17	0.5416029718947755
1101	18-19	0.5157545739945348
1101	20-21	0.511697084099076
1101	22-23	0.5319805375046371
1101	24-25	0.5911826484574618
1101	26-27	0.5737434651987456
1101	28-29	0.5595969272578714
1101	30-31	0.6198827579595871
1101	32-33	0.5801206368350833
1101	34-35	0.5040270111791969
1101	36-37	0.5541838521816729
1101	38-39	0.53637809712653
1101	40-41	0.5006247988789099
1101	42-43	0.48115297767430576
1101	44-45	0.4110883797225995
1101	46-47	0.43847925831585854
1101	48-49	0.420994942297078
1101	50-51	0.4103788326588429
1101	52-53	0.36701031979036003
1101	54-55	0.33747902840567434
1101	56-57	0.35755306083904514
1101	58-59	0.3249206701371179
1101	60-61	0.3734196752161054
1101	62-63	0.33614999446824356
1101	64-65	0.39103174765775606
1101	66-67	0.3371294497315134
1101	68-69	0.33017385684946277
1101	70-71	0.27636799793580025
1101	72-73	0.33088399141192326
1101	74-75	0.35590403770117973
1101	76-77	0.34310389852178247
1101	78-79	0.2939554752306677
1101	80-81	0.2801388877313187
1101	82-83	0.35047603315778275
1101	84-85	0.26077657166978696
1101	86-87	0.3134862138697585
1101	88-89	0.33071411225213154
1101	90-91	0.30732366589396065
1101	92-93	0.32465987562450493
1101	94-95	0.2897408305824172
1101	96-97	0.38936714456664845
1101	98-99	0.3349501053737072
1101	100	0.3484634291501294
1102	1	0.20553315508336567
1102	2	0.2954198563293069
1102	3	0.24265295952677945
1102	4	0.2737167386848611
1102	5	0.25463203720497063
1102	6	0.3021709464982152
1102	7	0.2730099498840062
1102	8	0.37147666934371415
1102	9	0.3942288331606889
1102	10-11	0.41221434474525154
1102	12-13	0.4269211650647904
1102	14-15	0.5044321882405853
1102	16-17	0.4933805021212265
1102	18-19	0.4850365245207655
1102	20-21	0.46317072389788905
1102	22-23	0.47416118315108235
1102	24-25	0.5174034346863365
1102	26-27	0.47766850084623513
1102	28-29	0.52058854490231
1102	30-31	0.5462360981552266
1102	32-33	0.5238788819782556
1102	34-35	0.4951115753485382
1102	36-37	0.4818651667865552
1102	38-39	0.4471382665749104
1102	40-41	0.41493034408660634
1102	42-43	0.48091639315335755
1102	44-45	0.3750422638169013
1102	46-47	0.4305769689743002
1102	48-49	0.33940351708617555
1102	50-51	0.3998309892625116
1102	52-53	0.32622539768838266
1102	54-55	0.2983627485533731
1102	56-57	0.33649013375860193
1102	58-59	0.31165260978917075
1102	60-61	0.2789739298976812
1102	62-63	0.22932291717459208
1102	64-65	0.3274312161815516
1102	66-67	0.27746373206632313
1102	68-69	0.25618208938173126
1102	70-71	0.23310778785815245
1102	72-73	0.2663847161508244
1102	74-75	0.2917638529074509
1102	76-77	0.27195751483068875
1102	78-79	0.20609773367045392
1102	80-81	0.17707740593762367
1102	82-83	0.2079105747149228
1102	84-85	0.23376434968901805
1102	86-87	0.17133912393775574
1102	88-89	0.22966322199150113
1102	90-91	0.25021949239104657
1102	92-93	0.23152764702364692
1102	94-95	0.26012473159448035
1102	96-97	0.24695385370566214
1102	98-99	0.24642473957224809
1102	100	0.25739815295861845
1103	1	0.09314713633898108
1103	2	0.1158399306870237
1103	3	0.09736995314477781
1103	4	0.11813646073182582
1103	5	0.0819253044035193
1103	6	0.1600096617258302
1103	7	0.13593348263795235
1103	8	0.2247008515417832
1103	9	0.26403762258789953
1103	10-11	0.2829206891615783
1103	12-13	0.24153503692604517
1103	14-15	0.35635508699915164
1103	16-17	0.3438141910408987
1103	18-19	0.35047945105659295
1103	20-21	0.2824009881414966
1103	22-23	0.3155569826183253
1103	24-25	0.37022684582982635
1103	26-27	0.33885755023614195
1103	28-29	0.35320633973447
1103	30-31	0.3946686549486458
1103	32-33	0.3535865700478453
1103	34-35	0.3725495968238022
1103	36-37	0.3713257342762617
1103	38-39	0.33683708011455593
1103	40-41	0.29843802517556384
1103	42-43	0.33799292013295457
1103	44-45	0.3190791700084219
1103	46-47	0.3406662507042526
1103	48-49	0.2758846869078013
1103	50-51	0.2679544730115353
1103	52-53	0.28963012802248755
1103	54-55	0.24820749698557165
1103	56-57	0.24881933536597955
1103	58-59	0.24107977568923644
1103	60-61	0.27958086950601313
1103	62-63	0.31338086128437936
1103	64-65	0.29222090083180063
1103	66-67	0.28073660295508773
1103	68-69	0.29575834082572783
1103	70-71	0.28678540032498745
1103	72-73	0.2604197366365071
1103	74-75	0.28505817750676954
1103	76-77	0.2022121073735761
1103	78-79	0.15740325994811855
1103	80-81	0.16528020762801532
1103	82-83	0.18777293458560962
1103	84-85	0.14657297151094184
1103	86-87	0.1802304319699104
1103	88-89	0.17418973823662043
1103	90-91	0.19967384993516646
1103	92-93	0.18816825678829474
1103	94-95	0.19859666801644238
1103	96-97	0.2261801831487844
1103	98-99	0.19623295977396538
1103	100	0.20060979931217204
1104	1	0.16476663482311693
1104	2	0.19655148083610996
1104	3	0.22557441307738202
1104	4	0.28947163064010084
1104	5	0.2105413258005342
1104	6	0.2752760668521219
1104	7	0.2818351542347628
1104	8	0.29924638497330847
1104	9	0.40530763400160197
1104	10-11	0.40767887825376903
1104	12-13	0.4086844209330067
1104	14-15	0.49415979924327047
1104	16-17	0.4554487132495879
1104	18-19	0.43768205888017064
1104	20-21	0.4690467501163056
1104	22-23	0.4753254013952244
1104	24-25	0.5043655752913878
1104	26-27	0.4837977691336448
1104	28-29	0.42920131664025973
1104	30-31	0.4541518164023586
1104	32-33	0.46481985554977
1104	34-35	0.45148427197903374
1104	36-37	0.48479909376363395
1104	38-39	0.39046136406547305
1104	40-41	0.4264602665308317
1104	42-43	0.43677224663506564
1104	44-45	0.3869269788764029
1104	46-47	0.414616740118376
1104	48-49	0.44582562297410533
1104	50-51	0.4657512342992334
1104	52-53	0.3889864388028812
1104	54-55	0.34809227323962233
1104	56-57	0.3919069025552062
1104	58-59	0.4249730108530656
1104	60-61	0.3816498025616326
1104	62-63	0.328463030967832
1104	64-65	0.387953743749911
1104	66-67	0.3638654515892199
1104	68-69	0.30293114163036705
1104	70-71	0.28526889057723537
1104	72-73	0.3565013956188423
1104	74-75	0.3006927595069939
1104	76-77	0.3052627648250237
1104	78-79	0.3202942000618876
1104	80-81	0.28841825470759375
1104	82-83	0.285601627745244
1104	84-85	0.23310892147905093
1104	86-87	0.245177059498765
1104	88-89	0.28624630466434553
1104	90-91	0.3115045356699824
1104	92-93	0.26499197581903644
1104	94-95	0.33351162965779935
1104	96-97	0.332507360884037
1104	98-99	0.31137324211206163
1104	100	0.38284075536192574
1105	1	0.08069642752501238
1105	2	0.17843832893467848
1105	3	0.07629870219924584
1105	4	0.20738887016211294
1105	5	0.08118642400119569
1105	6	0.16032705970575023
1105	7	0.1599864116889691
1105	8	0.21267725719080488
1105	9	0.22700506029536172
1105	10-11	0.2873125706617117
1105	12-13	0.31767868233797003
1105	14-15	0.3362447488254361
1105	16-17	0.32228609495656
1105	18-19	0.3274690181346287
1105	20-21	0.3463171485021519
1105	22-23	0.33206075695198933
1105	24-25	0.3800483935405765
1105	26-27	0.36628829563238696
1105	28-29	0.3283327032298331
1105	30-31	0.3558004265026824
1105	32-33	0.37586657062837503
1105	34-35	0.3546093542908082
1105	36-37	0.350927810029809
1105	38-39	0.3229396137963221
1105	40-41	0.3142886723403464
1105	42-43	0.3026939974123479
1105	44-45	0.24086667030086062
1105	46-47	0.2823324985781568
1105	48-49	0.24921367129491756
1105	50-51	0.3174416432230629
1105	52-53	0.2667822390412695
1105	54-55	0.19602693874637822
1105	56-57	0.234328798927109
1105	58-59	0.20896615100322435
1105	60-61	0.2769119591932281
1105	62-63	0.21258485357800794
1105	64-65	0.26009029879265455
1105	66-67	0.252515205706473
1105	68-69	0.18605786032602367
1105	70-71	0.13517950518144772
1105	72-73	0.15823223582418677
1105	74-75	0.18925004574994375
1105	76-77	0.220651056270178
1105	78-79	0.19465414504496437
1105	80-81	0.19531739642528123
1105	82-83	0.15031222889958684
1105	84-85	0.17999014282288783
1105	86-87	0.1866135647448388
1105	88-89	0.1632764952729957
1105	90-91	0.17787787992014614
1105	92-93	0.22318858303940914
1105	94-95	0.19233679028371142
1105	96-97	0.22890281446413496
1105	98-99	0.1886336853107693
1105	100	0.3069185187507735
1106	1	0.04837867777725435
1106	2	0.10623695997906069
1106	3	0.09083180252876488
1106	4	0.05371623026898931
1106	5	0.09001280588336158
1106	6	0.18973232003929752
1106	7	0.11167259160965415
1106	8	0.15889533562206992
1106	9	0.22002315605517708
1106	10-11	0.24317795366463457
1106	12-13	0.24500610602945727
1106	14-15	0.25553928809556936
1106	16-17	0.22019943682374077
1106	18-19	0.27681785237025736
1106	20-21	0.2843840821310053
1106	22-23	0.25456736695615234
1106	24-25	0.32094990797218514
1106	26-27	0.29973128329837095
1106	28-29	0.273055495306771
1106	30-31	0.2959463496813015
1106	32-33	0.31076580300146617
1106	34-35	0.26664607754543823
1106	36-37	0.2871600482638641
1106	38-39	0.283049269293528
1106	40-41	0.26512825117269045
1106	42-43	0.30719348174817895
1106	44-45	0.2748415501888388
1106	46-47	0.27150809270992937
1106	48-49	0.2633058717694965
1106	50-51	0.31048314650291076
1106	52-53	0.2739893096495294
1106	54-55	0.20974592902282296
1106	56-57	0.3107622125680294
1106	58-59	0.23302052213050217
1106	60-61	0.2437647272896868
1106	62-63	0.2344531544855215
1106	64-65	0.260944236566381
1106	66-67	0.24896599681077447
1106	68-69	0.23627231862709408
1106	70-71	0.2197429269835638
1106	72-73	0.2612245378943925
1106	74-75	0.26667015623343815
1106	76-77	0.20421033039948355
1106	78-79	0.224016870071587
1106	80-81	0.1547140833179057
1106	82-83	0.18737524194351352
1106	84-85	0.22148119486164575
1106	86-87	0.17342194240107034
1106	88-89	0.1658075643887571
1106	90-91	0.1828513665366387
1106	92-93	0.21473052023179662
1106	94-95	0.18049773829525861
1106	96-97	0.2561125716967183
1106	98-99	0.20732939383893267
1106	100	0.2859486997782419
1107	1	0.051665177352198555
1107	2	0.08451084968609734
1107	3	0.08754158920423905
1107	4	0.05160563885774394
1107	5	0.07806195972239749
1107	6	0.13378535778517175
1107	7	0.13764925710817266
1107	8	0.1314595041506763
1107	9	0.18220874495120398
1107	10-11	0.1500112022569553
1107	12-13	0.174647516498041
1107	14-15	0.1606647182996923
1107	16-17	0.17427807918492277
1107	18-19	0.132274160781634
1107	20-21	0.2193783562043734
1107	22-23	0.27450598205167154
1107	24-25	0.2814907278195449
1107	26-27	0.21671631958845694
1107	28-29	0.20866675823146608
1107	30-31	0.23845674649206217
1107	32-33	0.23824446075783356
1107	34-35	0.2150144079043983
1107	36-37	0.2134304713544708
1107	38-39	0.175763348388287
1107	40-41	0.21007655577847828
1107	42-43	0.2312191120221243
1107	44-45	0.17354016793123606
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2216	62-63	-0.48656674016469026
2216	64-65	-0.47624808384402684
2216	66-67	-0.4079276159622012
2216	68-69	-0.5131937749474318
2216	70-71	-0.4608930806271374
2216	72-73	-0.40007277944701514
2216	74-75	-0.39247496225337386
2216	76-77	-0.44965937530358246
2216	78-79	-0.3796402106659471
2216	80-81	-0.42117042122009707
2216	82-83	-0.3694803120038799
2216	84-85	-0.3731854457457686
2216	86-87	-0.42339669897544496
2216	88-89	-0.38297270439475994
2216	90-91	-0.4012849833657981
2216	92-93	-0.3769343328521586
2216	94-95	-0.39749050018065546
2216	96-97	-0.4078677475854029
2216	98-99	-0.3937396999648506
2216	100	-0.42515998735338556
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1787.0
1	484.0
2	321.0
3	328.0
4	271.0
5	337.0
6	458.0
7	889.0
8	2360.0
9	3652.0
10	4149.0
11	5489.0
12	7694.0
13	10254.0
14	13036.0
15	14591.0
16	16217.0
17	18276.0
18	19904.0
19	22364.0
20	24924.0
21	27863.0
22	31121.0
23	36649.0
24	43055.0
25	52976.0
26	59687.0
27	58950.0
28	62983.0
29	76862.0
30	91102.0
31	104719.0
32	131184.0
33	174235.0
34	254558.0
35	382736.0
36	690282.0
37	1413030.0
38	1759372.0
39	555973.0
40	1013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.951800898532824	20.027321284071178	13.432856623547615	24.588021193848384
2	14.761375277594475	20.455109933649513	40.79019828026304	23.99331650849297
3	17.89763552540558	26.00557458383857	30.370819527993078	25.72597036276278
4	12.100990262660243	17.56924627577948	40.15911289577692	30.17065056578335
5	13.14022688252457	38.35160552484308	35.575312731170705	12.932854861461651
6	29.787709870209973	38.652192577810574	18.72085299155244	12.839244560427016
7	26.345334172980493	32.73398817601347	23.497604704444093	17.423072946561945
8	25.0296602389446	35.95665270124924	21.058518464379922	17.955168595426237
9	26.141885971166133	15.741670922699214	20.97155177107125	37.1448913350634
10-11	24.262370205951207	26.59689276948363	29.21207301183121	19.92866401273395
12-13	24.830211631814613	24.33428450303064	28.45589293819892	22.379610926955824
14-15	22.32472631165062	25.678769224985633	25.92385540903588	26.07264905432787
16-17	22.192764471781583	28.33551025586949	26.822062286856784	22.649662985492146
18-19	22.049858201141536	28.277454382558194	27.889477344226442	21.783210072073828
20-21	23.26628249205109	27.42226912762884	27.23826163349366	22.073186746826412
22-23	23.129550804094723	27.375994398773525	26.841918880247288	22.65253591688446
24-25	22.820345069107397	27.51388771298443	27.2448470741314	22.42092014377677
26-27	22.680507917980076	27.746409463321108	26.885899116530183	22.687183502168633
28-29	22.510576990231527	27.952981107823966	26.99562757780968	22.54081432413482
30-31	22.927758286811592	27.640630697056366	26.868059212925075	22.56355180320696
32-33	22.870960352878367	27.80015853565752	26.565709772792296	22.76317133867182
34-35	22.97848976808115	27.80366844066437	26.932270160935172	22.28557163031931
36-37	22.937997978187894	27.438717669399686	26.985876161253614	22.6374081911588
38-39	22.90340417573791	27.57149307084986	27.07832368921132	22.446779064200907
40-41	22.899937512802097	27.233633734271244	27.25471668148901	22.611712071437644
42-43	22.684587284452547	27.29240813035035	27.09223825781633	22.930766327380773
44-45	22.765461436784275	27.69543610834469	27.05604675879999	22.48305569607104
46-47	22.759312472983325	27.507538335825515	26.799147458072753	22.934001733118404
48-49	22.868302389406555	27.241492280501618	27.108032137072886	22.78217319301894
50-51	22.73368505237297	27.420828778839162	26.893420379879252	22.952065788908616
52-53	22.60815800967707	27.4992362552277	26.905759972642944	22.986845762452283
54-55	22.66392210274175	27.387615446987873	26.927768104443167	23.02069434582721
56-57	22.81761800916898	27.338782711177036	26.926149299361075	22.917449980292908
58-59	22.70269696444925	27.29027312752626	27.203604693725747	22.803425214298745
60-61	22.715797712023477	27.45114786581697	27.01447920540339	22.818575216756162
62-63	22.682287570529034	27.24770101711238	26.90261553949468	23.16739587286391
64-65	22.374999706238704	27.257117957761746	27.10682562677923	23.26105670922032
66-67	22.676748481123138	27.211863163721027	26.8804048069201	23.230983548235738
68-69	22.443134162776204	27.201631116992615	27.092052599633227	23.263182120597957
70-71	22.52903644490071	26.84515271013167	27.1590812488842	23.46672959608342
72-73	22.69753558583392	27.03386433149248	27.19238015467144	23.076219928002164
74-75	22.583488092486597	27.232818816551973	27.121291736752656	23.062401354208774
76-77	22.556391464886353	27.090650331264506	27.05447525556751	23.298482948281634
78-79	22.477609997681355	27.075692143212056	27.138946346253263	23.30775151285333
80-81	22.43496314953279	27.061821019657227	27.30373869455057	23.199477136259407
82-83	22.68044303386097	26.819642142515722	27.63444211742475	22.865472706198556
84-85	22.88152618496952	27.034042247308438	27.03682192074786	23.04760964697418
86-87	22.393562605712923	27.173229160656415	27.272791365721815	23.160416867908847
88-89	22.737908729406573	26.717742707288387	27.19040259030614	23.3539459729989
90-91	22.729498647058474	26.673964795724913	27.335083231166816	23.261453326049793
92-93	22.86598153002829	26.725474390190147	27.2960164318701	23.112527647911467
94-95	22.390100351163845	26.73904783605623	27.518298207360314	23.352553605419608
96-97	22.5000034437586	26.621858370444222	27.42363019639877	23.454507989398408
98-99	22.32848923975536	26.86101877107535	27.255893479700767	23.554598509468523
100	22.769355173276832	26.60309108845774	27.014675707093545	23.612878031171878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1755.0
1	1117.5
2	477.0
3	470.0
4	444.5
5	417.5
6	411.0
7	402.5
8	429.5
9	459.0
10	431.5
11	430.5
12	457.0
13	497.5
14	563.5
15	643.5
16	718.5
17	908.5
18	1235.0
19	1604.0
20	2181.5
21	3275.5
22	5036.5
23	7833.0
24	11564.5
25	16723.5
26	23947.5
27	32631.5
28	41222.0
29	52908.0
30	68122.0
31	83521.0
32	101470.0
33	117571.0
34	130899.5
35	145008.5
36	162582.0
37	182945.5
38	191365.0
39	190182.5
40	191139.5
41	191655.5
42	195059.0
43	203661.5
44	213336.0
45	229610.5
46	243800.5
47	250994.5
48	256057.0
49	252873.0
50	245248.5
51	242610.5
52	239813.0
53	232743.0
54	222160.0
55	200533.0
56	177250.5
57	155339.0
58	134009.0
59	117274.0
60	96986.5
61	75429.5
62	59131.0
63	45824.5
64	32961.5
65	22823.0
66	16716.0
67	12477.0
68	9090.0
69	6675.0
70	5052.0
71	3614.5
72	2534.5
73	2017.5
74	1701.5
75	1285.5
76	854.5
77	534.5
78	310.5
79	205.5
80	155.0
81	118.0
82	88.0
83	65.5
84	52.5
85	41.5
86	39.5
87	38.0
88	23.5
89	18.0
90	15.0
91	13.5
92	11.5
93	12.0
94	10.5
95	7.0
96	6.5
97	6.0
98	8.5
99	10.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09478419756044841
2	0.06334058436222653
3	0.07953194028304109
4	0.08168539062050942
5	0.08257591519615423
6	0.08866386502238051
7	0.08563608146518818
8	0.08769238366713163
9	0.07290967571142794
10-11	0.07959670570672435
12-13	0.0689832719006304
14-15	0.07830949291101959
16-17	0.0803334124011214
18-19	0.07923240019850603
20-21	0.07084527783152408
22-23	0.07495788223541099
24-25	0.07711133257287932
26-27	0.07108814817033629
28-29	0.08024435994355693
30-31	0.08395218044942347
32-33	0.0855470290076237
34-35	0.08776524476877529
36-37	0.08796763671778547
38-39	0.0896515377335502
40-41	0.08534463705861352
42-43	0.09109256841050269
44-45	0.08748999171812145
46-47	0.08383074528001735
48-49	0.09315696629040654
50-51	0.08889054400527191
52-53	0.09520517281438959
54-55	0.08729569544707166
56-57	0.09337554959533753
58-59	0.09825724340546313
60-61	0.09731814476205589
62-63	0.09827343476138395
64-65	0.09973065679425724
66-67	0.1016817151827154
68-69	0.09925301179459323
70-71	0.10134979238633869
72-73	0.09858107052387942
74-75	0.10253985704651858
76-77	0.09943111670972218
78-79	0.10661198306060343
80-81	0.10147932323370523
82-83	0.10660388738264304
84-85	0.10274224899552875
86-87	0.09913967230314752
88-89	0.0980143730666509
90-91	0.0981277125580966
92-93	0.09208024112167237
94-95	0.09153783069832509
96-97	0.08988631239440201
98-99	0.10453948950273917
100	0.24398754237075454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6176135.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.01175601008966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.4429768238412	35.07827655155761
2	15.531795179536717	16.15671882553501
3	6.756175524762726	10.542016588658951
4	3.5734262894408357	7.434407051457471
5	2.004605599673549	5.2131528673340055
6	1.241464414058372	3.874244651952778
7	0.8516566270648944	3.1007309683892648
8	0.5836837384946604	2.4286732954913006
9	0.4023579637685353	1.8834609816221
>10	1.5770435619830911	12.703547285447481
>50	0.027421149737031355	0.9458939394711504
>100	0.007143371416425134	0.5590843230485656
>500	2.497562219514485E-4	0.07979267003431427
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2667898289140378E-4
2	0.0	0.0	0.0	0.0	2.2667898289140378E-4
3	0.0	0.0	0.0	0.0	2.2667898289140378E-4
4	0.0	0.0	0.0	0.0	2.2667898289140378E-4
5	0.0	0.0	0.0	0.0	2.2667898289140378E-4
6	0.0	0.0	0.0	0.0	2.5906169473303287E-4
7	0.0	0.0	0.0	0.0	2.752530506538474E-4
8	0.0	0.0	0.0	0.0	2.91444406574662E-4
9	0.0	0.0	0.0	1.6191355920814554E-5	2.91444406574662E-4
10-11	0.0	0.0	0.0	1.6191355920814554E-5	3.319227963766984E-4
12-13	0.0	0.0	0.0	1.6191355920814554E-5	0.0010848208466945751
14-15	0.0	0.0	0.0	1.6191355920814554E-5	0.0013519782193880153
16-17	0.0	0.0	0.0	3.238271184162911E-5	0.0016110399141210483
18-19	0.0	0.0	0.0	6.476542368325822E-5	0.0017729534733291938
20-21	0.0	0.0	0.0	6.476542368325822E-5	0.002040110846022634
22-23	0.0	0.0	0.0	8.905245756448005E-5	0.002137258981547521
24-25	0.0	0.0	0.0	2.1048762697058922E-4	0.002137258981547521
26-27	0.0	0.0	0.0	5.019320335452512E-4	0.002250598472993223
28-29	0.0	0.0	0.0	0.0019186756766165249	0.0022991725407556667
30-31	0.0	0.0	0.0	0.00847617482454642	0.0022991725407556667
32-33	0.0	0.0	0.0	0.025938552185144917	0.0024610860999638123
34-35	0.0	0.0	0.0	0.0528485857255387	0.0025825212693699213
36-37	0.0	0.0	0.0	0.09751244103310566	0.0025906169473303288
38-39	0.0	0.0	0.0	0.16992828038894875	0.002776817540419696
40-41	0.0	0.0	0.0	0.249840717536129	0.0027849132183801035
42-43	0.0	0.0	0.0	0.33335573137569047	0.0028496786420633617
44-45	0.0	0.0	0.0	0.4398786619787294	0.0029873051673902857
46-47	0.0	0.0	0.0	0.5702190771412865	0.003100644658835987
48-49	0.0	0.0	0.0	0.7130673147526729	0.003238271184162911
50-51	0.0	0.0	0.0	0.866917578712253	0.0034325674552126858
52-53	0.0	0.0	0.0	1.0232855985175195	0.0036916291499457187
54-55	0.0	0.0	0.0	1.197545066615286	0.00380496864139142
56-57	0.0	0.0	0.0	1.4043167774020484	0.003877829743035086
58-59	0.0	0.0	0.0	1.6373022934246095	0.003958786522639158
60-61	0.0	0.0	0.0	1.8870134801133718	0.003999264912441195
62-63	0.0	0.0	0.0	2.1423673543405384	0.0043230920308574864
64-65	0.0	0.0	0.0	2.4105204954231083	0.004379761776580338
66-67	0.0	0.0	0.0	2.7168123753771574	0.004412144488421966
68-69	0.0	0.0	0.0	3.0582314020014136	0.004436431522303188
70-71	0.0	0.0	0.0	3.4284791378426798	0.004476909912105224
72-73	0.0	0.0	0.0	3.8050253111371433	0.004695493217036221
74-75	0.0	0.0	0.0	4.189432387731162	0.004930267877888032
76-77	0.0	0.0	0.0	4.614083079466365	0.005043607369333734
78-79	0.0	0.0	0.0	5.087324030319934	0.005237903640383509
80-81	0.0	0.0	0.0	5.594672072420696	0.005294573386106359
82-83	0.0	0.0	0.0	6.122906963659311	0.00552934804695817
84-85	1.6191355920814554E-5	0.0	0.0	6.6470697288838405	0.005569826436760207
86-87	1.6191355920814554E-5	0.0	0.0	7.191787420449844	0.005788409741691203
88	1.6191355920814554E-5	0.0	0.0	7.626063873279972	0.0060069930466221996
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4900	0.0	40.977905	1
GTATCAA	8025	0.0	35.392387	1
CAACGCA	8285	0.0	32.6795	5
ATCAACG	8715	0.0	31.284859	3
AACGCAG	9860	0.0	28.548233	6
TCAACGC	9900	0.0	27.824831	4
TATCAAC	10245	0.0	27.530647	2
ACGCAGA	10310	0.0	26.345018	7
CGCAGAG	10760	0.0	25.286491	8
GTACATG	18350	0.0	25.036665	1
TACATGG	17905	0.0	24.442886	2
ACATGGG	18125	0.0	22.978905	3
TGATCGC	1760	0.0	22.69381	9
CATGGGG	14380	0.0	21.215628	4
GTATAAG	3035	0.0	20.761755	1
GTGATCG	1945	0.0	20.053066	8
GCAGAGT	13275	0.0	19.928518	9
TAACGCC	1200	0.0	19.194859	4
CTAACGC	1180	0.0	19.12198	3
GTACTAG	2925	0.0	18.487999	1
>>END_MODULE