FastQCFastQC Report
Mon 16 Jan 2017
ERR1146615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6176135
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133250.21574981764485393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120010.19431246240569547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.10058070298010001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.057.6574671
GTATCAA179100.043.4598541
CAACGCA221850.034.363665
ATCAACG222400.034.3404053
TATCAAC237150.032.6215022
TCAACGC237300.032.2832684
AACGCAG240000.032.234136
ACGCAGA262850.029.0016487
CGCAGAG266600.028.5584558
GTGGTAT31300.025.5440881
GCAGAGT301100.025.005079
GTACATG184350.023.1137771
TACATGG180950.022.7930372
CAGAGTA289450.022.06592810-11
ACATGGG183900.021.9923113
GAGTACT204250.021.98602512-13
AGAGTAC274450.021.87630710-11
TGGTATC35200.021.1093862
GTACTTT220250.020.20756114-15
CATGGGG145900.020.048844
Produced by FastQC (version 0.11.5)