##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6176135
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2682046295944	33.0	31.0	34.0	30.0	34.0
2	32.383610299969156	34.0	31.0	34.0	30.0	34.0
3	32.52975493573246	34.0	31.0	34.0	31.0	34.0
4	36.055227257823866	37.0	35.0	37.0	35.0	37.0
5	36.070277123152266	37.0	35.0	37.0	35.0	37.0
6	36.13928872992575	37.0	36.0	37.0	35.0	37.0
7	36.1136955393624	37.0	36.0	37.0	35.0	37.0
8	36.11824304358632	37.0	36.0	37.0	35.0	37.0
9	37.9053493811259	39.0	38.0	39.0	35.0	39.0
10-11	37.85494819980457	39.0	38.0	39.0	35.0	39.0
12-13	37.82244907211387	39.0	38.0	39.0	35.0	39.0
14-15	39.19268652644413	40.0	39.0	41.0	36.0	41.0
16-17	39.13799665972327	40.0	39.0	41.0	36.0	41.0
18-19	39.2335132732688	40.5	39.0	41.0	36.0	41.0
20-21	39.28863374910037	41.0	39.0	41.0	36.0	41.0
22-23	39.25752124913073	41.0	39.0	41.0	36.0	41.0
24-25	39.24810095634244	41.0	39.0	41.0	36.0	41.0
26-27	39.17518197060136	41.0	39.0	41.0	36.0	41.0
28-29	39.11998798601391	40.0	39.0	41.0	35.0	41.0
30-31	38.99141752892383	40.0	39.0	41.0	35.0	41.0
32-33	38.89894076149566	40.0	38.0	41.0	35.0	41.0
34-35	38.83093115678333	40.0	38.0	41.0	35.0	41.0
36-37	38.76953288747736	40.0	38.0	41.0	35.0	41.0
38-39	38.687204457156454	40.0	38.0	41.0	35.0	41.0
40-41	38.594305176295535	40.0	38.0	41.0	35.0	41.0
42-43	38.496474737032145	40.0	38.0	41.0	34.5	41.0
44-45	38.366440662323605	40.0	38.0	41.0	34.0	41.0
46-47	38.151217873313975	40.0	38.0	41.0	33.5	41.0
48-49	37.88501279521901	40.0	38.0	41.0	33.0	41.0
50-51	37.78540972630941	40.0	37.0	41.0	33.0	41.0
52-53	37.687434860151214	40.0	37.0	41.0	33.0	41.0
54-55	37.532543734876256	40.0	37.0	41.0	33.0	41.0
56-57	37.385316221228976	40.0	36.0	41.0	33.0	41.0
58-59	37.20931521088836	39.0	36.0	41.0	32.0	41.0
60-61	37.01441581183053	39.0	35.0	41.0	32.0	41.0
62-63	36.77780594174188	39.0	35.0	41.0	32.0	41.0
64-65	36.51020160666825	38.5	35.0	41.0	31.0	41.0
66-67	36.25157359740355	37.5	35.0	40.0	31.0	41.0
68-69	35.966708953091214	37.0	35.0	40.0	31.0	41.0
70-71	35.661887005384436	36.5	35.0	39.0	31.0	41.0
72-73	35.341689827052036	36.0	35.0	39.0	31.0	41.0
74-75	35.02133551161042	35.5	35.0	39.0	30.5	40.5
76-77	34.7158996686439	35.0	35.0	37.5	30.0	39.5
78-79	34.42594000940718	35.0	34.0	37.0	30.0	39.0
80-81	34.15793793367535	35.0	34.0	36.5	30.0	39.0
82-83	33.90041522732259	35.0	34.0	36.0	30.0	38.0
84-85	33.68225629783028	35.0	34.0	36.0	30.0	37.0
86-87	33.49134491069253	35.0	34.0	35.5	29.5	37.0
88-89	33.29435075496245	35.0	34.0	35.0	29.0	36.0
90-91	33.20677608245286	35.0	34.0	35.0	29.0	36.0
92-93	33.109320149251914	35.0	34.0	35.0	29.0	36.0
94-95	33.035857214908674	35.0	34.0	35.0	29.0	36.0
96-97	32.981441224973224	35.0	34.0	35.0	29.0	35.5
98-99	32.91136957012759	35.0	34.0	35.0	29.0	35.0
100	32.18646386453664	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23386763558335844
1101	2	0.30377354088106756
1101	3	0.2581827513342816
1101	4	0.21131295469707823
1101	5	0.19795606481859807
1101	6	0.23591040003159236
1101	7	0.22964315182905892
1101	8	0.264100091178868
1101	9	0.2860376380735872
1101	10-11	0.2907166594851702
1101	12-13	0.31479619511137713
1101	14-15	0.45764909143603205
1101	16-17	0.4906375186641796
1101	18-19	0.4241786825717142
1101	20-21	0.3828561116786844
1101	22-23	0.3697813569674224
1101	24-25	0.34755152964115155
1101	26-27	0.32723683484521615
1101	28-29	0.4171793562112782
1101	30-31	0.4626261198027919
1101	32-33	0.44031967507572745
1101	34-35	0.4007466720427999
1101	36-37	0.4088936486940966
1101	38-39	0.37737981701693
1101	40-41	0.4066924240681402
1101	42-43	0.40146910911039413
1101	44-45	0.36783669297440014
1101	46-47	0.3834897528860921
1101	48-49	0.5211894319812984
1101	50-51	0.49225142701376967
1101	52-53	0.4676540758396399
1101	54-55	0.44281612411424476
1101	56-57	0.42061784494960364
1101	58-59	0.45302494644399616
1101	60-61	0.44385141021804486
1101	62-63	0.398031019134109
1101	64-65	0.38821406984634166
1101	66-67	0.3698697211684916
1101	68-69	0.3388187555047253
1101	70-71	0.32073076573080783
1101	72-73	0.3484753420455746
1101	74-75	0.31889111852094487
1101	76-77	0.3333911725248768
1101	78-79	0.30178921193781605
1101	80-81	0.2545071350200061
1101	82-83	0.2489574355602926
1101	84-85	0.25486550957849374
1101	86-87	0.29323815136956455
1101	88-89	0.35567604807100395
1101	90-91	0.3265816111671427
1101	92-93	0.34664484194198053
1101	94-95	0.3441544706892472
1101	96-97	0.3215930170429715
1101	98-99	0.36636364384752795
1101	100	0.4590864923133111
1102	1	0.23994880603947877
1102	2	0.2307349408342816
1102	3	0.2601073881967153
1102	4	0.14770223198043908
1102	5	0.19328793397018273
1102	6	0.21910380833728027
1102	7	0.20955903610445148
1102	8	0.2050508832568454
1102	9	0.28269304181149124
1102	10-11	0.30274704192473223
1102	12-13	0.3200919616429658
1102	14-15	0.4275080908003517
1102	16-17	0.4760254809458573
1102	18-19	0.37497375309887104
1102	20-21	0.36489086187886954
1102	22-23	0.3542941012492662
1102	24-25	0.346176971011559
1102	26-27	0.3488461541255319
1102	28-29	0.3464379687969128
1102	30-31	0.4264767422086777
1102	32-33	0.37824473158811855
1102	34-35	0.3581943461288617
1102	36-37	0.37190075750643103
1102	38-39	0.3632614288140701
1102	40-41	0.34227452797253477
1102	42-43	0.373314510887532
1102	44-45	0.32962881295653546
1102	46-47	0.30440521452651836
1102	48-49	0.4136396434751646
1102	50-51	0.3640982217947766
1102	52-53	0.36334567467249457
1102	54-55	0.34087097511905995
1102	56-57	0.28345740735703373
1102	58-59	0.2911303456998624
1102	60-61	0.23367620741763773
1102	62-63	0.24878567747322222
1102	64-65	0.2612750167506732
1102	66-67	0.2464031749554536
1102	68-69	0.2025183771956236
1102	70-71	0.18683487944310428
1102	72-73	0.20562339818555841
1102	74-75	0.20090357764404132
1102	76-77	0.23492872919066343
1102	78-79	0.2085388644481725
1102	80-81	0.18650337710563036
1102	82-83	0.1690493515035456
1102	84-85	0.15262461862528198
1102	86-87	0.20639118186004168
1102	88-89	0.28480876265533084
1102	90-91	0.2507465784645717
1102	92-93	0.24247748241669598
1102	94-95	0.2642594875022297
1102	96-97	0.2616695484343836
1102	98-99	0.23719582978689147
1102	100	0.2999020454246448
1103	1	0.15766931093546077
1103	2	0.165268445903493
1103	3	0.19579354560490003
1103	4	0.13944333815422993
1103	5	0.10235167281889801
1103	6	0.14177642860286
1103	7	0.16569792806135553
1103	8	0.1915255995137528
1103	9	0.2509887924026444
1103	10-11	0.2386694689054636
1103	12-13	0.2396756405426359
1103	14-15	0.35653043020702313
1103	16-17	0.40865153891760286
1103	18-19	0.34377805864552613
1103	20-21	0.3289302317860745
1103	22-23	0.34766231682222326
1103	24-25	0.2947589407121285
1103	26-27	0.3181274202704216
1103	28-29	0.3045843176077483
1103	30-31	0.3465430915301795
1103	32-33	0.3572287595424797
1103	34-35	0.29919050772191014
1103	36-37	0.2767687862071071
1103	38-39	0.3271098710043674
1103	40-41	0.3673717062421247
1103	42-43	0.36421630227295054
1103	44-45	0.3426639303042478
1103	46-47	0.35728100671330054
1103	48-49	0.4625840317843952
1103	50-51	0.4295938075447907
1103	52-53	0.3865300181136391
1103	54-55	0.3801691581536346
1103	56-57	0.36579919810061057
1103	58-59	0.365760434045761
1103	60-61	0.31582314677064005
1103	62-63	0.27995368660238285
1103	64-65	0.2843204504319772
1103	66-67	0.26247874409017413
1103	68-69	0.27095733309084125
1103	70-71	0.29583504599425936
1103	72-73	0.2357628045536444
1103	74-75	0.25048327899274625
1103	76-77	0.24017547145813012
1103	78-79	0.17799059078844692
1103	80-81	0.21572654711302164
1103	82-83	0.21095278064206013
1103	84-85	0.18898477532392377
1103	86-87	0.17115263948488746
1103	88-89	0.21453484514125876
1103	90-91	0.19069242532627584
1103	92-93	0.20342066387419067
1103	94-95	0.19465218457342814
1103	96-97	0.21107982321218088
1103	98-99	0.24378082294155234
1103	100	0.272322777554038
1104	1	0.16502603393679038
1104	2	0.19178926553345121
1104	3	0.1733311182429702
1104	4	0.1230989926070265
1104	5	0.13407851965632034
1104	6	0.14922452880073678
1104	7	0.14717975047024368
1104	8	0.1796927677680884
1104	9	0.2212891152165355
1104	10-11	0.20210213112451214
1104	12-13	0.18762478646053893
1104	14-15	0.3518448106284069
1104	16-17	0.3530489819388194
1104	18-19	0.3192431371139648
1104	20-21	0.29152315391808514
1104	22-23	0.2926958555919015
1104	24-25	0.2747848623798177
1104	26-27	0.28211799035256746
1104	28-29	0.2704059625094004
1104	30-31	0.2943731626980508
1104	32-33	0.29452989976392985
1104	34-35	0.25829557146666815
1104	36-37	0.2692566697639549
1104	38-39	0.2595525567152208
1104	40-41	0.2683797089665987
1104	42-43	0.2738667316702106
1104	44-45	0.2580014258178167
1104	46-47	0.3270189771409946
1104	48-49	0.4242867441057854
1104	50-51	0.3363752801127049
1104	52-53	0.3337209503742713
1104	54-55	0.35214975112637603
1104	56-57	0.3325328495234885
1104	58-59	0.2780263862840755
1104	60-61	0.3000742986234144
1104	62-63	0.2760556612456
1104	64-65	0.35052154632085575
1104	66-67	0.2879889121423318
1104	68-69	0.2420517966961171
1104	70-71	0.24458041414172982
1104	72-73	0.2382416943741532
1104	74-75	0.2791557904843458
1104	76-77	0.25752962364288834
1104	78-79	0.24107919471249772
1104	80-81	0.2670063829148859
1104	82-83	0.24126242225385397
1104	84-85	0.2797186632201658
1104	86-87	0.2542602478324696
1104	88-89	0.288496002784683
1104	90-91	0.24072600174045533
1104	92-93	0.2318740816726077
1104	94-95	0.2603790216837325
1104	96-97	0.2726496092590196
1104	98-99	0.2419933484127199
1104	100	0.3014252125042134
1105	1	0.13376259832289605
1105	2	0.12965667415883075
1105	3	0.17000735606161044
1105	4	0.12349042071387828
1105	5	0.09992225961687495
1105	6	0.14712491761301294
1105	7	0.1455560179677846
1105	8	0.17257779033421627
1105	9	0.20017744280109184
1105	10-11	0.19395965004921578
1105	12-13	0.2046876985249284
1105	14-15	0.292067826523315
1105	16-17	0.3663958546723265
1105	18-19	0.2779517429791838
1105	20-21	0.22467146681459127
1105	22-23	0.2693336562774391
1105	24-25	0.2820161781009105
1105	26-27	0.26398385219075493
1105	28-29	0.2501378107292993
1105	30-31	0.32266351019008255
1105	32-33	0.3004435207309655
1105	34-35	0.2682069194881933
1105	36-37	0.2358808484350874
1105	38-39	0.27845751542504615
1105	40-41	0.3374265796468521
1105	42-43	0.3171307370630174
1105	44-45	0.3363318649743121
1105	46-47	0.3256700687272627
1105	48-49	0.4108947878555185
1105	50-51	0.4067824726731857
1105	52-53	0.31616230110029164
1105	54-55	0.27457717003463955
1105	56-57	0.2551637988625828
1105	58-59	0.24648543038493642
1105	60-61	0.24209545723298476
1105	62-63	0.2715189476561264
1105	64-65	0.27544966157370965
1105	66-67	0.24756090364848404
1105	68-69	0.18148695285878347
1105	70-71	0.17264791321414208
1105	72-73	0.20898751495845147
1105	74-75	0.16242686587477095
1105	76-77	0.20059701020093001
1105	78-79	0.174363069258888
1105	80-81	0.1963449895765308
1105	82-83	0.13899106075550094
1105	84-85	0.1653870397075039
1105	86-87	0.2078460608715602
1105	88-89	0.24685812795374318
1105	90-91	0.18810008910385534
1105	92-93	0.1956369671587126
1105	94-95	0.22589232850238972
1105	96-97	0.2197029918242066
1105	98-99	0.21555799060566727
1105	100	0.2552644096847416
1106	1	0.06842137956670058
1106	2	0.05000105817072864
1106	3	0.07572710585967002
1106	4	0.07649842678446106
1106	5	0.09862419035297876
1106	6	0.09104329454255833
1106	7	0.09368355611155721
1106	8	0.1057667889395475
1106	9	0.1102246141944434
1106	10-11	0.11002613509216275
1106	12-13	0.12895869402229465
1106	14-15	0.20850123317692493
1106	16-17	0.2743263923325827
1106	18-19	0.2156468638737863
1106	20-21	0.17971033727248908
1106	22-23	0.12794341378950946
1106	24-25	0.1343425012895878
1106	26-27	0.1592856834274201
1106	28-29	0.16299074023283566
1106	30-31	0.17470859377239378
1106	32-33	0.15220570521855592
1106	34-35	0.15680005067371638
1106	36-37	0.17251590563328278
1106	38-39	0.14793500018570427
1106	40-41	0.11644596526583229
1106	42-43	0.12653683936855487
1106	44-45	0.13670598352396013
1106	46-47	0.14355999958075927
1106	48-49	0.22565955276016325
1106	50-51	0.19762038502985035
1106	52-53	0.17624725642336614
1106	54-55	0.15916621783070184
1106	56-57	0.1795460674215974
1106	58-59	0.17521503439957087
1106	60-61	0.16291008784134675
1106	62-63	0.13957110659808336
1106	64-65	0.182698236859288
1106	66-67	0.1293577312657277
1106	68-69	0.11535232398326656
1106	70-71	0.14197847211584502
1106	72-73	0.15429680515859445
1106	74-75	0.2027017749697464
1106	76-77	0.14520416214159582
1106	78-79	0.19965908704580926
1106	80-81	0.18934290945153975
1106	82-83	0.08956852776836399
1106	84-85	0.12046460244664559
1106	86-87	0.10404864492203103
1106	88-89	0.1526773597119444
1106	90-91	0.13196527506437405
1106	92-93	0.1494434491551786
1106	94-95	0.15631548405968942
1106	96-97	0.16741487180442505
1106	98-99	0.17184925072680102
1106	100	0.21889331594565675
1107	1	0.07376597163831633
1107	2	0.06472691930485297
1107	3	0.10061176017373441
1107	4	0.03326633026478021
1107	5	0.06619083358926048
1107	6	0.09853949807764906
1107	7	0.07911552933475008
1107	8	0.08098882052262724
1107	9	0.12320467541054114
1107	10-11	0.14395284120559637
1107	12-13	0.13549101073356695
1107	14-15	0.20219731206506708
1107	16-17	0.2701658111510241
1107	18-19	0.19316677801189286
1107	20-21	0.1553895820713862
1107	22-23	0.16151596323808803
1107	24-25	0.16250023503805267
1107	26-27	0.18856640440801442
1107	28-29	0.20428940807710205
1107	30-31	0.24355062301918906
1107	32-33	0.1960330145124516
1107	34-35	0.1498712911943656
1107	36-37	0.11659941300642629
1107	38-39	0.16471785657230953
1107	40-41	0.14911193779894205
1107	42-43	0.20022168247614047
1107	44-45	0.2484212260351839
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2216	42-43	-0.515185867884334
2216	44-45	-0.531540040768185
2216	46-47	-0.47610034190806516
2216	48-49	-0.3902421165458776
2216	50-51	-0.4119655264961892
2216	52-53	-0.4263245799400579
2216	54-55	-0.3827140730327514
2216	56-57	-0.41737188081417287
2216	58-59	-0.42280836293034696
2216	60-61	-0.47347619629669424
2216	62-63	-0.4310781304822626
2216	64-65	-0.35646564164405703
2216	66-67	-0.432361085588127
2216	68-69	-0.37508734393036747
2216	70-71	-0.371570443379305
2216	72-73	-0.34670993133276795
2216	74-75	-0.32326881752244674
2216	76-77	-0.36555802064430054
2216	78-79	-0.3047990910325211
2216	80-81	-0.3403926939591244
2216	82-83	-0.32309049526343614
2216	84-85	-0.316460289345855
2216	86-87	-0.30091838837006435
2216	88-89	-0.21683085212014674
2216	90-91	-0.2739368538000093
2216	92-93	-0.29854656416672753
2216	94-95	-0.2802840193003746
2216	96-97	-0.3018375720423023
2216	98-99	-0.33014665867576554
2216	100	-0.4432071720313431
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	42.0
8	203.0
9	315.0
10	277.0
11	231.0
12	267.0
13	417.0
14	712.0
15	1260.0
16	2379.0
17	4237.0
18	7259.0
19	11334.0
20	16153.0
21	24333.0
22	35118.0
23	40492.0
24	38342.0
25	33882.0
26	33121.0
27	38651.0
28	47167.0
29	58786.0
30	73437.0
31	93233.0
32	119985.0
33	158009.0
34	227812.0
35	348744.0
36	626628.0
37	1428026.0
38	2000566.0
39	702972.0
40	1745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39747026137092	20.244790538302137	13.6949572650403	24.662781935286645
2	14.981725752213764	20.72598602568952	40.19717599237294	24.095112229723778
3	17.601627688054087	26.181315718828092	30.898078114389584	25.318978478728237
4	12.215807705099177	17.975119622185534	39.5071778171213	30.301894855593986
5	12.7400324061458	38.26650274150423	35.58596040245538	13.407504449894583
6	29.41762148861872	39.15474900118947	18.145125380393228	13.282504129798573
7	25.746357105061872	33.292552685078626	23.358649070324276	17.60244113953523
8	25.17715017010691	35.59193188728968	20.796889177599642	18.434028765003767
9	26.181491528519295	15.0828171798086	21.046337401358194	37.68935389031391
10-11	24.289619415147943	26.393940149537105	29.011883824909013	20.304556610405943
12-13	25.269484880107058	24.199883907978048	28.632963819605628	21.897667392309266
14-15	22.46840477221134	25.23481328640967	26.765747582568082	25.53103435881091
16-17	21.632264401619818	28.112061303217985	27.51681699164014	22.73885730352206
18-19	21.44273136936741	27.219508411867604	29.80147505098463	21.53628516778035
20-21	22.53644705964453	26.410216907374874	29.444316577304942	21.60901945567566
22-23	22.358554338595255	26.409518250491608	29.1019302524961	22.129997158417037
24-25	22.087478657768976	26.552050756662542	29.471408898931127	21.889061686637355
26-27	22.011050310583542	26.773533722488374	29.078679869354783	22.136736097573298
28-29	21.797841352062523	26.936265717658834	29.251556940672195	22.014335989606444
30-31	22.27261161471269	26.642512896641396	29.055936138582723	22.02893935006319
32-33	22.300411407361615	26.767595238237877	28.767379244920395	22.164614109480116
34-35	22.378076868586692	26.78479629217349	29.039511020259468	21.797615818980347
36-37	22.361050573400956	26.515406406469292	28.98365028467656	22.13989273545319
38-39	22.39654829041769	26.591497505695372	29.02935847553541	21.982595728351527
40-41	22.416181765559507	26.374691096912084	29.070013985391235	22.139113152137174
42-43	22.322763346332295	26.3886233056758	28.814752268206572	22.473861079785333
44-45	22.377187756457165	26.891643429932248	28.573221194686717	22.15794761892387
46-47	22.57692715589928	26.752718326267157	28.046002880442217	22.624351637391346
48-49	22.825114946557967	26.65876096216223	28.055509185281206	22.460614905998593
50-51	22.564969839551758	26.966533276879474	27.703329023734096	22.76516785983467
52-53	22.461326030364056	27.09226896247772	27.686299063586546	22.760105943571673
54-55	22.53339475269263	26.959231685912837	27.658546142238166	22.848827419156365
56-57	22.66704414148052	26.894068526250948	27.611556720110876	22.827330612157663
58-59	22.47756448477633	26.849092779500687	27.93309585296651	22.740246882756477
60-61	22.55907084421061	27.04338870105913	27.730416001940384	22.667124452789874
62-63	22.453114011365216	26.83324683088127	27.611477454595118	23.102161703158398
64-65	22.126608373288327	26.792817686714432	27.827578044194766	23.252995895802474
66-67	22.39694316960922	26.732653067998495	27.672983979052702	23.197419783339583
68-69	22.274884048944706	26.67698835951713	27.825241607033174	23.22288598450499
70-71	22.313129859948948	26.336469515950363	27.81427207723408	23.53612854686661
72-73	22.450726656731362	26.52583664779995	27.80408409645406	23.21935259901463
74-75	22.313157031885652	26.815935945336566	27.6183598541121	23.25254716866568
76-77	22.356882315861434	26.64693560220335	27.483067592483597	23.513114489451624
78-79	22.184995114195065	26.6920307542598	27.529086123747277	23.593888007797858
80-81	22.094234513676618	26.79843923413763	27.607335440876202	23.499990811309555
82-83	22.364845719541005	26.52024986682513	27.95001368179544	23.164890731838426
84-85	22.5975986186779	26.697046620916197	27.287207550640275	23.41814720976563
86-87	22.034306244925023	26.870178517794706	27.53841196800264	23.55710326927763
88-89	22.334753045391658	26.51687503592457	27.40770724733187	23.740664671351905
90-91	22.228112278651984	26.499384566461803	27.530927923519794	23.74157523136642
92-93	22.296903124918536	26.58037097346898	27.42152082639466	23.70120507521783
94-95	21.830119277816074	26.586311568211634	27.63559366285751	23.947975491114782
96-97	21.89901411411905	26.45612241535767	27.55482992924118	24.090033541282104
98-99	21.734839715737433	26.681520650259284	27.366521802276868	24.217117831726416
100	22.003739446854176	26.442254485578143	27.14773926148598	24.406266806081696
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	57.5
2	134.0
3	219.0
4	151.0
5	149.5
6	320.5
7	811.0
8	1540.5
9	1988.0
10	1745.0
11	1448.0
12	1620.5
13	1947.5
14	2460.5
15	3076.5
16	3739.5
17	4515.0
18	5431.0
19	6354.5
20	7364.0
21	8781.0
22	10602.0
23	13403.0
24	17217.0
25	22367.0
26	29443.0
27	37440.0
28	45394.0
29	57017.5
30	71754.5
31	85597.0
32	102090.0
33	117683.0
34	129550.0
35	142369.0
36	159695.5
37	179613.5
38	188145.0
39	186953.0
40	187752.0
41	188438.0
42	191273.5
43	199174.0
44	209216.5
45	225959.0
46	240036.0
47	247623.0
48	253224.0
49	250592.0
50	243541.0
51	240079.0
52	236360.5
53	229473.5
54	220184.0
55	198897.5
56	175579.5
57	153907.0
58	132845.0
59	117004.5
60	96288.0
61	74416.5
62	57955.0
63	44243.5
64	31915.5
65	21935.0
66	15729.5
67	11771.5
68	8525.5
69	6181.5
70	4597.5
71	3267.0
72	2262.0
73	1740.5
74	1400.0
75	1079.0
76	671.5
77	355.0
78	189.5
79	90.0
80	59.0
81	36.5
82	17.5
83	10.5
84	3.5
85	1.5
86	2.0
87	0.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026068083032511433
2	0.0037402032177081624
3	2.104876269705892E-4
4	0.05113230199793236
5	0.002363937964438925
6	0.004679301861115407
7	4.8574067762443666E-5
8	3.0763576249547654E-4
9	1.6191355920814554E-5
10-11	1.45722203287331E-4
12-13	0.0
14-15	7.28611016436655E-5
16-17	2.509660167726256E-4
18-19	1.5381788124773827E-4
20-21	3.157314404558838E-4
22-23	0.0
24-25	0.0
26-27	3.0763576249547654E-4
28-29	8.095677960407277E-6
30-31	1.8620059308936738E-4
32-33	2.91444406574662E-4
34-35	1.6191355920814555E-4
36-37	4.8574067762443666E-5
38-39	0.001052438134852946
40-41	7.690894062386914E-4
42-43	0.0
44-45	4.938363555848439E-4
46-47	0.0
48-49	6.476542368325822E-5
50-51	0.0
52-53	2.752530506538474E-4
54-55	2.4287033881221834E-4
56-57	8.905245756448005E-5
58-59	7.367066943970622E-4
60-61	5.505061013076948E-4
62-63	1.5381788124773827E-4
64-65	3.0763576249547654E-4
66-67	0.0010443424568925387
68-69	0.002355842286478518
70-71	0.003375897709489835
72-73	0.0028982527098258054
74-75	0.0025906169473303288
76-77	0.004144987115728526
78-79	0.0012953084736651644
80-81	0.0010443424568925387
82-83	7.28611016436655E-4
84-85	3.7240118617873477E-4
86-87	0.0
88-89	0.0
90-91	1.6191355920814554E-5
92-93	9.714813552488733E-5
94-95	1.2143516940610917E-4
96-97	2.3477466085181106E-4
98-99	2.2667898289140375E-4
100	0.07503074333705465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6176135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.4667104620334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85944090035599	32.5784989420931
2	16.257981628385362	16.08457739816794
3	7.035651543155827	10.440916133911431
4	3.7342310566868444	7.388805059178445
5	2.1247744636302746	5.255280159476055
6	1.3427693426608034	3.9853429374418123
7	0.8844763154518135	3.062649366488668
8	0.6082958610781257	2.407231618816395
9	0.44772933031715834	1.9932927433343086
>10	1.6589037103911444	12.823289564888473
>50	0.03121392690910615	1.0459859185691798
>100	0.012785559155302433	1.1831662223818233
>500	0.001034881080579158	0.3611287131442906
>1k	5.174405394894973E-4	0.38077370515683445
>5k	6.468006743618717E-5	0.1987535716691249
>10k+	1.2936013487237433E-4	0.8103079452820763
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13325	0.21574981764485393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12001	0.19431246240569547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6212	0.10058070298010001	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9429627104977466E-4
2	0.0	0.0	0.0	0.0	1.9429627104977466E-4
3	0.0	0.0	0.0	0.0	1.9429627104977466E-4
4	0.0	0.0	0.0	0.0	1.9429627104977466E-4
5	0.0	0.0	0.0	0.0	2.5906169473303287E-4
6	0.0	0.0	0.0	0.0	2.91444406574662E-4
7	0.0	0.0	0.0	0.0	2.91444406574662E-4
8	0.0	0.0	0.0	0.0	2.91444406574662E-4
9	0.0	0.0	0.0	6.476542368325822E-5	2.91444406574662E-4
10-11	0.0	0.0	0.0	6.476542368325822E-5	2.91444406574662E-4
12-13	0.0	0.0	0.0	6.476542368325822E-5	0.0011576819483382406
14-15	0.0	0.0	0.0	6.476542368325822E-5	0.0014653177108337172
16-17	0.0	0.0	0.0	6.476542368325822E-5	0.0017810491512896011
18-19	0.0	0.0	0.0	8.095677960407277E-5	0.0019348670325373392
20-21	0.0	0.0	0.0	8.905245756448005E-5	0.002193928727270372
22-23	0.0	0.0	0.0	1.2953084736651643E-4	0.002250598472993223
24-25	0.0	0.0	0.0	2.752530506538474E-4	0.0022667898289140376
26-27	0.0	0.0	0.0	5.909844911097312E-4	0.0024125120322013686
28-29	0.0	0.0	0.0	0.0020077281341810047	0.0024448947440429978
30-31	0.0	0.0	0.0	0.008605705671912937	0.0024610860999638123
32-33	0.0	0.0	0.0	0.02616523116803632	0.0025744255914095142
34-35	0.0	0.0	0.0	0.053237178267638255	0.0026796694048948086
36-37	0.0	0.0	0.0	0.0981277125580966	0.0026958607608156236
38-39	0.0	0.0	0.0	0.17094833581196006	0.0028496786420633617
40-41	0.0	0.0	0.0	0.25108745194203175	0.002882061353904991
42-43	0.0	0.0	0.0	0.3345457960358703	0.0029225397437070274
44-45	0.0	0.0	0.0	0.4409877698593052	0.003027783557192322
46-47	0.0	0.0	0.0	0.5712715152761395	0.003173505760479653
48-49	0.0	0.0	0.0	0.7146135892431108	0.00327065389600454
50-51	0.0	0.0	0.0	0.8692572296428106	0.0034811415229751294
52-53	0.0	0.0	0.0	1.0262486166510285	0.0037159161838269403
54-55	0.0	0.0	0.0	1.2017872018665394	0.0037321075397477544
56-57	0.0	0.0	0.0	1.4107933197703741	0.0037887772854706056
58-59	0.0	0.0	0.0	1.6459970515540867	0.0038373513532330493
60-61	0.0	0.0	0.0	1.8973435651908515	0.0039021167769163075
62-63	0.0	0.0	0.0	2.1547051675521987	0.004080221692045268
64-65	0.0	0.0	0.0	2.4253679688024956	0.00410450872592649
66-67	0.0	0.0	0.0	2.7348981199407074	0.004136891437768119
68-69	0.0	0.0	0.0	3.0801625935961567	0.004144987115728526
70-71	0.0	0.0	0.0	3.454352924604142	0.00419356118349097
72-73	0.0	0.0	0.0	3.83497931959065	0.004452622878224003
74-75	0.0	0.0	0.0	4.223045642622774	0.004768354318679887
76-77	0.0	0.0	0.0	4.650821266050693	0.0048978851660464034
78-79	0.0	0.0	0.0	5.12682284308876	0.004986937623610883
80-81	0.0	0.0	0.0	5.636210996035546	0.005067894403214955
82-83	0.0	0.0	0.0	6.16542546430737	0.005335051775908395
84-85	0.0	0.0	0.0	6.690648763344713	0.005416008555512468
86-87	0.0	0.0	0.0	7.237236556519571	0.005723644318007945
88	0.0	0.0	0.0	7.672403533925343	0.0059908016907013854
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9560	0.0	57.657467	1
GTATCAA	17910	0.0	43.459854	1
CAACGCA	22185	0.0	34.36366	5
ATCAACG	22240	0.0	34.340405	3
TATCAAC	23715	0.0	32.621502	2
TCAACGC	23730	0.0	32.283268	4
AACGCAG	24000	0.0	32.23413	6
ACGCAGA	26285	0.0	29.001648	7
CGCAGAG	26660	0.0	28.558455	8
GTGGTAT	3130	0.0	25.544088	1
GCAGAGT	30110	0.0	25.00507	9
GTACATG	18435	0.0	23.113777	1
TACATGG	18095	0.0	22.793037	2
CAGAGTA	28945	0.0	22.065928	10-11
ACATGGG	18390	0.0	21.992311	3
GAGTACT	20425	0.0	21.986025	12-13
AGAGTAC	27445	0.0	21.876307	10-11
TGGTATC	3520	0.0	21.109386	2
GTACTTT	22025	0.0	20.207561	14-15
CATGGGG	14590	0.0	20.04884	4
>>END_MODULE