FastQCFastQC Report
Mon 16 Jan 2017
ERR1146614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669187
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1122824.206599237895285No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT273551.0248438944142917No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC209970.7866440230676982No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA179370.6720023737565034No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG177260.66409734499681No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC150810.5650035010660549No Hit
GTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGAGT38370.14375163673433147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6950.029.0971431
GAGTACG14750.027.1015171
GTACGCA13900.026.0442733
AGTACGC14550.024.8807832
TAGACCG1201.8351956E-423.5070515
TACGCAG15250.023.429984
GGCGTCG5850.022.496158
TACGGGC5650.022.4669194
TATTCGC1050.002336551722.3805339
CGTTAAC2607.0940587E-1021.705741
TAACGCC4700.021.0063044
CGGGCGT6050.020.9759946
CGCAGTG17400.020.7996316
TACCTGG17350.020.323482
ACGGGCG6300.020.1489015
GTATAAG9700.019.878281
TCTAACG4500.019.850772
GGACCGT1909.045592E-619.7902166
TGATCGC4650.019.203949
TAGGCAT10800.019.1538965
Produced by FastQC (version 0.11.5)