##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146614_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2669187
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282218518222965	31.0	31.0	34.0	28.0	34.0
2	31.302217866339078	31.0	31.0	34.0	28.0	34.0
3	31.476391125837193	33.0	31.0	34.0	28.0	34.0
4	34.88420818773657	37.0	35.0	37.0	32.0	37.0
5	34.95376045215266	37.0	35.0	37.0	32.0	37.0
6	35.15440881436932	37.0	35.0	37.0	32.0	37.0
7	35.078550884595195	37.0	35.0	37.0	32.0	37.0
8	35.07870448941944	37.0	35.0	37.0	32.0	37.0
9	36.67908805190494	39.0	37.0	39.0	32.0	39.0
10-11	36.682420527299136	39.0	37.0	39.0	32.0	39.0
12-13	36.657471732029265	39.0	37.0	39.0	32.0	39.0
14-15	37.861738237148614	40.0	38.0	41.0	32.0	41.0
16-17	37.95635393848389	40.0	38.0	41.0	32.5	41.0
18-19	38.01802440218688	40.0	38.0	41.0	32.0	41.0
20-21	38.083846879218285	40.0	38.0	41.0	32.5	41.0
22-23	38.04256296018225	40.0	38.0	41.0	32.0	41.0
24-25	37.974524827222666	40.0	38.0	41.0	32.0	41.0
26-27	37.92325959177832	40.0	38.0	41.0	32.0	41.0
28-29	37.95155622292481	40.0	38.0	41.0	32.0	41.0
30-31	37.97793654022742	40.0	38.0	41.0	32.0	41.0
32-33	37.87855140909947	40.0	38.0	41.0	32.0	41.0
34-35	37.71220281681276	40.0	38.0	41.0	31.0	41.0
36-37	37.56744993887652	40.0	37.5	41.0	30.5	41.0
38-39	37.48021026627209	40.0	37.0	41.0	31.0	41.0
40-41	37.561268843284495	40.0	37.0	41.0	31.0	41.0
42-43	37.38112204203003	40.0	37.0	41.0	30.0	41.0
44-45	37.17590899401203	40.0	37.0	41.0	30.0	41.0
46-47	36.95796603984659	40.0	36.0	41.0	30.0	41.0
48-49	36.7425937186117	39.0	36.0	41.0	29.5	41.0
50-51	36.52537289444314	39.0	35.0	41.0	28.5	41.0
52-53	36.54039095050291	39.0	35.0	41.0	29.0	41.0
54-55	36.41559077726664	39.0	35.0	41.0	28.5	41.0
56-57	36.00797096644034	38.5	34.5	40.0	28.0	41.0
58-59	35.75063361989999	38.0	34.0	40.0	28.0	41.0
60-61	35.351163668937396	38.0	34.0	40.0	27.0	41.0
62-63	35.244326455958316	37.5	34.0	40.0	26.5	41.0
64-65	35.00678671071004	37.0	34.0	39.5	26.5	41.0
66-67	34.62252288805543	36.5	33.5	39.0	26.0	41.0
68-69	34.18741961503633	36.0	33.0	39.0	26.0	40.5
70-71	33.66031866632049	35.0	32.0	38.0	25.5	40.0
72-73	33.25399082192443	35.0	32.0	37.0	25.0	40.0
74-75	33.0269400382963	35.0	32.0	37.0	26.0	39.0
76-77	32.84004099375578	35.0	32.0	36.5	26.0	39.0
78-79	32.44324095689062	35.0	31.5	36.0	25.0	38.5
80-81	32.02826216372251	35.0	31.0	35.5	24.0	37.0
82-83	31.74558788874665	34.5	30.5	35.0	23.5	37.0
84-85	31.590956160059225	34.0	31.0	35.0	23.0	36.5
86-87	31.634233195351243	34.5	31.0	35.0	24.0	36.0
88-89	31.483131005808136	34.0	31.0	35.0	23.0	36.0
90-91	31.1889236310532	34.0	31.0	35.0	21.0	36.0
92-93	30.929575934544864	34.0	30.0	35.0	20.0	35.0
94-95	30.801166797230767	34.0	30.0	35.0	20.0	35.0
96-97	30.697941358173857	34.0	30.0	35.0	19.0	35.0
98-99	30.781586865214013	34.0	30.0	35.0	19.5	35.0
100	29.452662926951167	34.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30415470093858943
1101	2	0.3610335028703169
1101	3	0.36756290802847147
1101	4	0.3192415771377597
1101	5	0.26264538758541534
1101	6	0.3219043440999485
1101	7	0.35610129383263
1101	8	0.38673116809540176
1101	9	0.527904188720612
1101	10-11	0.5226476270025557
1101	12-13	0.517632072277209
1101	14-15	0.6580765273987552
1101	16-17	0.670656742438986
1101	18-19	0.6036834621568303
1101	20-21	0.5969025507451136
1101	22-23	0.5897089979869818
1101	24-25	0.5975791639667989
1101	26-27	0.6546788861897213
1101	28-29	0.6533338017531136
1101	30-31	0.5506615881739165
1101	32-33	0.5927634177068484
1101	34-35	0.6571471832727411
1101	36-37	0.5872309294239813
1101	38-39	0.5476920863531305
1101	40-41	0.5420323750150402
1101	42-43	0.5845709493340507
1101	44-45	0.5762374798871548
1101	46-47	0.5847401537561581
1101	48-49	0.6438681295459858
1101	50-51	0.6369640304638224
1101	52-53	0.5859782395745938
1101	54-55	0.6527587794905187
1101	56-57	0.6873193184842279
1101	58-59	0.7176282167336225
1101	60-61	0.7654677079869927
1101	62-63	0.7718892439807803
1101	64-65	0.6530283286093521
1101	66-67	0.6283334119320756
1101	68-69	0.6485032443970482
1101	70-71	0.6074994382757595
1101	72-73	0.6767659573163058
1101	74-75	0.6160357619403598
1101	76-77	0.5495720560734654
1101	78-79	0.5690757333867609
1101	80-81	0.56203847916791
1101	82-83	0.5183299652335229
1101	84-85	0.485851705075639
1101	86-87	0.4847368534881369
1101	88-89	0.4275792122320681
1101	90-91	0.3611379827131387
1101	92-93	0.3959858637174172
1101	94-95	0.411421321306797
1101	96-97	0.4401793512154555
1101	98-99	0.4917228061984247
1101	100	0.49112437198373016
1102	1	0.2817084969842192
1102	2	0.2762400027069809
1102	3	0.2716104277340108
1102	4	0.26787989758204134
1102	5	0.1843394333908961
1102	6	0.26455822844452115
1102	7	0.3318438296616222
1102	8	0.3284451377632607
1102	9	0.3769574818180885
1102	10-11	0.42183830095306973
1102	12-13	0.4452510413467081
1102	14-15	0.5161647745840341
1102	16-17	0.4583839181344658
1102	18-19	0.4090804515835984
1102	20-21	0.46284471332512567
1102	22-23	0.44508310408154017
1102	24-25	0.47608217356970783
1102	26-27	0.5015775882277183
1102	28-29	0.528630961632409
1102	30-31	0.5360350150459041
1102	32-33	0.4707338844621347
1102	34-35	0.46303248521951446
1102	36-37	0.5301040247706652
1102	38-39	0.5517854535829088
1102	40-41	0.5357054166707869
1102	42-43	0.5679772182350362
1102	44-45	0.48404635635597515
1102	46-47	0.48769735949915116
1102	48-49	0.5220638717907207
1102	50-51	0.6043264151172778
1102	52-53	0.5496766227014049
1102	54-55	0.5365251172193908
1102	56-57	0.5996055655207115
1102	58-59	0.5839772659837266
1102	60-61	0.5868113881443122
1102	62-63	0.4139391862468642
1102	64-65	0.4762654225717142
1102	66-67	0.5466793755710313
1102	68-69	0.5294963324986881
1102	70-71	0.5187835894254178
1102	72-73	0.57704823898667
1102	74-75	0.5272447134470966
1102	76-77	0.525607223631205
1102	78-79	0.4229362243029655
1102	80-81	0.4557559937307616
1102	82-83	0.4214737146068508
1102	84-85	0.4549784512436901
1102	86-87	0.44691927650149665
1102	88-89	0.5089266281135849
1102	90-91	0.4219066524395423
1102	92-93	0.5422742357502663
1102	94-95	0.4246707706513462
1102	96-97	0.5672217088293792
1102	98-99	0.5582191012009616
1102	100	0.56714668282833
1103	1	0.21423985521158784
1103	2	0.2192922963988515
1103	3	0.2464228230693557
1103	4	0.23680700411260602
1103	5	0.20819885783346592
1103	6	0.23469000773732063
1103	7	0.2009107213018808
1103	8	0.27285538014189825
1103	9	0.37161942606618936
1103	10-11	0.2937469725701689
1103	12-13	0.40106561421357867
1103	14-15	0.42677688388877044
1103	16-17	0.4552762804331465
1103	18-19	0.4391686105750736
1103	20-21	0.3139073841771136
1103	22-23	0.3341705549635847
1103	24-25	0.4310011590097602
1103	26-27	0.41490797004944824
1103	28-29	0.4413824418218084
1103	30-31	0.4226461413579301
1103	32-33	0.46037430040992433
1103	34-35	0.4682272406494121
1103	36-37	0.45748385517908474
1103	38-39	0.47556409147930623
1103	40-41	0.41400164076272716
1103	42-43	0.4574025728597917
1103	44-45	0.3665389839106723
1103	46-47	0.4417484675194032
1103	48-49	0.555366233253352
1103	50-51	0.47833598029501445
1103	52-53	0.4014198513257483
1103	54-55	0.28572849410335976
1103	56-57	0.4040888229761279
1103	58-59	0.4828305150533012
1103	60-61	0.5219992651587546
1103	62-63	0.44370400904215757
1103	64-65	0.3767005171847586
1103	66-67	0.36263679273235994
1103	68-69	0.33483733342848154
1103	70-71	0.3649121372563755
1103	72-73	0.30749289117699163
1103	74-75	0.4063274267096446
1103	76-77	0.27765491397837394
1103	78-79	0.30432425180648437
1103	80-81	0.25373040805205704
1103	82-83	0.4331591037376974
1103	84-85	0.3781918049969235
1103	86-87	0.3299086257248547
1103	88-89	0.339012506967336
1103	90-91	0.38447945850369436
1103	92-93	0.32036062383477315
1103	94-95	0.5039791858796292
1103	96-97	0.4396079426245656
1103	98-99	0.2764037378863513
1103	100	0.2894224249498194
1104	1	0.22898448841691632
1104	2	0.21292468932526631
1104	3	0.22296818413824937
1104	4	0.2963073773375413
1104	5	0.2096366731635655
1104	6	0.2863570041609549
1104	7	0.31320391122090996
1104	8	0.3179940129695069
1104	9	0.4569247060748225
1104	10-11	0.45222461204987496
1104	12-13	0.4661769896298935
1104	14-15	0.5142979433679287
1104	16-17	0.4482007451026888
1104	18-19	0.47593145385956603
1104	20-21	0.4397984024966135
1104	22-23	0.45389968959135985
1104	24-25	0.48820953393743594
1104	26-27	0.5035906359246951
1104	28-29	0.46184423647915196
1104	30-31	0.4631051855423678
1104	32-33	0.48633265660141944
1104	34-35	0.5508551563755191
1104	36-37	0.5101842649770632
1104	38-39	0.5616636735872831
1104	40-41	0.5467340294922352
1104	42-43	0.5271430241471933
1104	44-45	0.5365652370621063
1104	46-47	0.5820986544316469
1104	48-49	0.5893781164954106
1104	50-51	0.5746853832850078
1104	52-53	0.5369451691665006
1104	54-55	0.5017903190383848
1104	56-57	0.5807301133451332
1104	58-59	0.6661124737497417
1104	60-61	0.6299525161127946
1104	62-63	0.5982447250344691
1104	64-65	0.68663013655528
1104	66-67	0.593221601728132
1104	68-69	0.6001294006749163
1104	70-71	0.713191367963347
1104	72-73	0.6530460435037355
1104	74-75	0.5650097028721106
1104	76-77	0.6212226295719816
1104	78-79	0.6196630096419611
1104	80-81	0.612923507737591
1104	82-83	0.5337244870501969
1104	84-85	0.47853763761828816
1104	86-87	0.5382119062071382
1104	88-89	0.45784833746026266
1104	90-91	0.5554330407136057
1104	92-93	0.5631418315846624
1104	94-95	0.5804493398761323
1104	96-97	0.6198897124601999
1104	98-99	0.5636969652777637
1104	100	0.5534575560581914
1105	1	0.1219230609242743
1105	2	0.014563102990866383
1105	3	0.14080168672919058
1105	4	0.20053256807784692
1105	5	0.11546903001068642
1105	6	0.0814553037729624
1105	7	0.12685076172738263
1105	8	0.05594365232060028
1105	9	0.2250178238000018
1105	10-11	0.19628835706978975
1105	12-13	0.22775186659262658
1105	14-15	0.3055356070921178
1105	16-17	0.2844494756706055
1105	18-19	0.2415763253539822
1105	20-21	0.2631668893335757
1105	22-23	0.2640155659551837
1105	24-25	0.3682288032653531
1105	26-27	0.19688490980954754
1105	28-29	0.2833170380831902
1105	30-31	0.260471537129483
1105	32-33	0.31697505433075435
1105	34-35	0.29150875114676467
1105	36-37	0.27235232476405713
1105	38-39	0.39598859611938764
1105	40-41	0.3341308474491669
1105	42-43	0.31722921533017256
1105	44-45	0.35504556411988375
1105	46-47	0.4150044170022795
1105	48-49	0.35653901538351107
1105	50-51	0.3405177401321353
1105	52-53	0.3092405627555408
1105	54-55	0.3591900013548681
1105	56-57	0.3397801875580839
1105	58-59	0.26492662889387475
1105	60-61	0.42890520210087146
1105	62-63	0.3828152012993513
1105	64-65	0.3429575068128159
1105	66-67	0.378044662426511
1105	68-69	0.3816657389818232
1105	70-71	0.30503367195001374
1105	72-73	0.2898702499459347
1105	74-75	0.21346112593801791
1105	76-77	0.24396564444061397
1105	78-79	0.2551607572137655
1105	80-81	0.204166900180887
1105	82-83	0.2317704730408714
1105	84-85	0.28912118577858337
1105	86-87	0.18529156144240488
1105	88-89	0.2004866730069672
1105	90-91	0.28233875120497487
1105	92-93	0.33075842403837896
1105	94-95	0.2567751477557252
1105	96-97	0.23743990385149516
1105	98-99	0.23008448708595708
1105	100	0.3465518455668253
1106	1	0.11885416364058798
1106	2	0.1523378826274886
1106	3	0.18133715473902612
1106	4	0.15783963769468556
1106	5	0.06353402971087263
1106	6	0.13518858913604248
1106	7	0.12197758309812912
1106	8	0.14449555014395088
1106	9	0.2494154972424667
1106	10-11	0.16895910120842927
1106	12-13	0.23084654789023062
1106	14-15	0.2555206163863559
1106	16-17	0.3255455960759619
1106	18-19	0.34348823996692346
1106	20-21	0.356323332338917
1106	22-23	0.30156458556302823
1106	24-25	0.41283244900499483
1106	26-27	0.35194157467733334
1106	28-29	0.32138263741160955
1106	30-31	0.31095933469499215
1106	32-33	0.3352493242833887
1106	34-35	0.2921905284448414
1106	36-37	0.35339297955808746
1106	38-39	0.2944748346514885
1106	40-41	0.29264406925164366
1106	42-43	0.35308878239858643
1106	44-45	0.3563782378621667
1106	46-47	0.37217027417084125
1106	48-49	0.37860713315049566
1106	50-51	0.3892861037466915
1106	52-53	0.33384990496337963
1106	54-55	0.30215156518529085
1106	56-57	0.3537860039519174
1106	58-59	0.3350867296314135
1106	60-61	0.332222945100213
1106	62-63	0.43120280098753483
1106	64-65	0.4995693074963867
1106	66-67	0.42268458567409084
1106	68-69	0.36364181381542693
1106	70-71	0.3557529260124994
1106	72-73	0.4396819666815617
1106	74-75	0.3221110629770507
1106	76-77	0.2348003269374601
1106	78-79	0.29717041122827936
1106	80-81	0.2831301429703572
1106	82-83	0.2929226586857716
1106	84-85	0.3336414831941852
1106	86-87	0.2952483882098065
1106	88-89	0.26839696862368356
1106	90-91	0.24582498973708056
1106	92-93	0.2928637187556511
1106	94-95	0.2343694392949729
1106	96-97	0.19686935759017388
1106	98-99	0.2798209504786513
1106	100	0.24007885881860958
1107	1	0.07910785206947679
1107	2	0.10229736765184683
1107	3	0.14360990736110324
1107	4	0.11421645548908543
1107	5	-0.009788399466224007
1107	6	0.11247798447485735
1107	7	0.06305573218254779
1107	8	0.030836273704238693
1107	9	0.0825037289789563
1107	10-11	0.1427168690191678
1107	12-13	0.20160745529326363
1107	14-15	0.23121112169798863
1107	16-17	0.22225367154331366
1107	18-19	0.2299817859644051
1107	20-21	0.2752239249882109
1107	22-23	0.2440278769833526
1107	24-25	0.3342855678814374
1107	26-27	0.32894429664197133
1107	28-29	0.3105775404188549
1107	30-31	0.35449272058829706
1107	32-33	0.3561024408170397
1107	34-35	0.33805642209290454
1107	36-37	0.2923338745494348
1107	38-39	0.28783859750722485
1107	40-41	0.3221553519466056
1107	42-43	0.31836196054250365
1107	44-45	0.25835125478653254
1107	46-47	0.27874184269118274
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2216	84-85	-1.2066557807457308
2216	86-87	-1.2691251779309383
2216	88-89	-1.1618968815077366
2216	90-91	-1.2766542230339013
2216	92-93	-1.2904690177580136
2216	94-95	-1.4119576131081857
2216	96-97	-1.2621052584757315
2216	98-99	-1.1817722118777816
2216	100	-1.1637526040818855
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	815.0
1	207.0
2	158.0
3	149.0
4	125.0
5	167.0
6	215.0
7	607.0
8	1655.0
9	2257.0
10	2655.0
11	3357.0
12	4290.0
13	5398.0
14	6593.0
15	7255.0
16	7860.0
17	8700.0
18	9708.0
19	10749.0
20	12186.0
21	13631.0
22	15676.0
23	18521.0
24	21810.0
25	26046.0
26	29553.0
27	30814.0
28	35116.0
29	42714.0
30	50871.0
31	61857.0
32	79407.0
33	107252.0
34	155853.0
35	238593.0
36	386110.0
37	591151.0
38	544511.0
39	134396.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.3528997050944	18.480726133796544	13.231076390451419	20.935297770657645
2	13.50453457614309	24.78328054990733	35.000547328138964	26.711637545810618
3	25.84404136510392	24.01953597924921	30.169597525982546	19.966825129664326
4	11.081019468039075	16.152450771230107	43.29135917823443	29.475170582496386
5	11.169419986254336	41.54808563705675	34.881851412604995	12.400642964083925
6	27.560193356709835	38.68296646781269	16.6897469271005	17.067093248376977
7	24.675012036591237	33.37814245024501	23.151877033283988	18.79496847987977
8	24.25431101881673	28.209345256107532	20.891365738110693	26.644977986965046
9	21.95928580507021	20.742741431456714	24.782292620763062	32.51568014271001
10-11	25.999915261418522	23.72685905574224	27.569772473159244	22.703453209679996
12-13	30.35243738369544	22.825190225383643	25.671218029191728	21.151154361729187
14-15	23.60129978498768	25.215532554334715	29.840706411731432	21.342461248946172
16-17	20.488142606676796	28.514211324365547	28.747204753755867	22.25044131520179
18-19	19.733185154939594	33.223418110346785	25.857185949073802	21.18621078563982
20-21	23.25531697523603	26.789756990495007	23.549024147235997	26.40590188703296
22-23	21.423868757116608	27.21165486954934	25.668750142941395	25.69572623039266
24-25	26.351718564258388	27.064197738701147	24.16180822921414	22.42227546782633
26-27	23.88650367299087	23.84164457610778	30.5937728525043	21.67807889839705
28-29	20.146512249192682	32.18950042416374	26.298976851439583	21.365010475203995
30-31	22.42073353527767	26.49839805032535	25.224993827117675	25.855874587279303
32-33	23.407399907944086	27.014454278115725	26.68093439563231	22.89721141830788
34-35	26.23617980313069	25.669113236884204	26.143784232343364	21.950922727641746
36-37	24.18553914937474	24.143878412144687	30.310342493633236	21.360239944847333
38-39	22.50045889457687	28.02986199038562	28.431083034325315	21.038596080712196
40-41	21.783174690133446	29.469912538596805	26.635333626939968	22.11157914432978
42-43	20.97861660927617	29.09159261214116	23.233880425886568	26.695910352696107
44-45	23.21103673060424	25.313431839590095	24.734172601441145	26.74135882836452
46-47	26.05689877864058	27.807970793717697	25.94598030203349	20.189150125608236
48-49	24.505521827616374	26.818505519577556	26.71661543411664	21.959357218689423
50-51	18.7624639721362	27.888885305581535	31.377522512961782	21.971128209320483
52-53	21.94031977708983	30.188614855552785	24.6931703758984	23.177894991458984
54-55	20.486972408121755	26.305674707501193	26.65743075031414	26.54992213406291
56-57	24.774024912293203	23.914282660586412	26.337240371940574	24.97445205517981
58-59	28.23260295118093	24.730635172202227	25.147011503650162	21.889750372966684
60-61	23.715910850961617	26.39884627832504	30.104845263511997	19.780397607201348
62-63	20.883125221383224	27.2042643588995	29.100795488873228	22.811814930844054
64-65	19.694894792633356	32.024008180242866	25.667462176408897	22.61363485071488
66-67	22.720196342653036	26.566115065525686	23.819073125561864	26.89461546625942
68-69	20.89363155179698	26.798089358654785	25.93352658765854	26.3747525018897
70-71	25.4600585627102	26.26375347030469	24.56217201460036	23.71401595238475
72-73	23.81501713050896	23.521667360787777	30.346399874139372	22.316915634563895
74-75	20.618225732652252	31.104529054809216	26.120731039552346	22.156514172986192
76-77	21.679301881340304	25.926676688830565	26.102080183436726	26.291941246392405
78-79	22.894897278147035	26.53583014518312	26.984155857648197	23.585116719021652
80-81	25.29979389609145	25.002836249310157	26.357091382733888	23.3402784718645
82-83	23.376769990000966	23.72729870852708	30.363997032545235	22.53193426892671
84-85	22.234521509490715	27.294266604834778	28.021360130319522	22.449851755354988
86-87	21.419005479941138	28.52839653592114	26.9684772124156	23.084120771722123
88-89	20.77754972006155	28.177720507080682	24.063249701197723	26.98148007166004
90-91	22.6570349238185	24.85410437279975	25.319627776292247	27.169232927089503
92-93	25.102704729761406	26.989321708151692	26.17664651010172	21.731327051985186
94-95	23.255398394825523	25.96357719455222	27.047662476852775	23.73336193376949
96-97	19.163053372995968	27.000542814365925	30.86433109613814	22.972072716499962
98-99	22.18511092508581	28.69571511725595	25.150620484385875	23.968553473272365
100	21.620543606488667	22.423232456392384	25.69577071011973	30.260453226999218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1706.0
1	1137.5
2	602.0
3	607.0
4	533.0
5	475.5
6	424.5
7	389.5
8	407.5
9	423.0
10	388.0
11	336.0
12	319.5
13	344.0
14	380.0
15	413.0
16	492.5
17	560.5
18	669.5
19	832.5
20	1040.5
21	1437.0
22	1980.5
23	2811.0
24	3779.5
25	5014.5
26	7101.0
27	9554.5
28	11726.0
29	15063.5
30	20109.0
31	25037.5
32	30747.0
33	36167.0
34	40723.0
35	45387.5
36	50430.0
37	55901.5
38	58220.5
39	58159.0
40	57479.5
41	56882.5
42	56718.5
43	58680.0
44	63259.5
45	72863.5
46	97724.5
47	181243.5
48	333930.5
49	329292.5
50	186997.0
51	113502.0
52	81742.0
53	70363.0
54	65035.5
55	57960.5
56	51844.5
57	45436.5
58	38519.5
59	33755.0
60	28684.0
61	23043.5
62	18332.0
63	14353.5
64	10571.5
65	7384.0
66	5283.0
67	3965.0
68	3014.5
69	2247.0
70	1804.0
71	1468.0
72	1125.0
73	966.5
74	785.0
75	569.5
76	433.5
77	293.0
78	170.5
79	98.5
80	69.5
81	53.5
82	35.0
83	30.5
84	29.0
85	20.5
86	16.5
87	13.0
88	14.0
89	12.5
90	10.5
91	12.0
92	9.0
93	7.5
94	9.0
95	6.0
96	6.5
97	8.5
98	7.5
99	7.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09632146417617049
2	0.06305290712115712
3	0.07897535841437861
4	0.08051140665678351
5	0.08107337552595603
6	0.09013980661527274
7	0.08688038717407211
8	0.08834150623392066
9	0.07343059890520971
10-11	0.08096098175212153
12-13	0.07082680981137703
14-15	0.07981831171813739
16-17	0.0808860525695652
18-19	0.07985577630941557
20-21	0.07135131408927138
22-23	0.07530382846911812
24-25	0.07835719265828883
26-27	0.07292482692295445
28-29	0.0814667537343768
30-31	0.08543800040986263
32-33	0.08727376538249287
34-35	0.08963403463301747
36-37	0.09019600350219
38-39	0.09188191010970757
40-41	0.0881729155731689
42-43	0.09420471476895399
44-45	0.09062684630188893
46-47	0.0885475614859506
48-49	0.09695836222789936
50-51	0.09369894278669873
52-53	0.09898145015692045
54-55	0.09081416925827976
56-57	0.09860680424413876
58-59	0.10224086959812109
60-61	0.10062989217315985
62-63	0.10166016843330948
64-65	0.10349593340593971
66-67	0.10594986413465972
68-69	0.10312128749315802
70-71	0.10544409215240445
72-73	0.10285903535421086
74-75	0.10666169136894492
76-77	0.10353339799721788
78-79	0.10990237851450649
80-81	0.1054066275611263
82-83	0.10973378785375473
84-85	0.10555648592623897
86-87	0.10246565714579009
88-89	0.10145411318127953
90-91	0.10212847582428658
92-93	0.09598428285466698
94-95	0.09594681826338881
96-97	0.09476668363812651
98-99	0.10801041665495897
100	0.24861502772192431
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2669187.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.7101868673612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3471736302054	42.72952296496459
2	13.843808354122485	15.701699174364444
3	5.012100289011827	8.527114319634473
4	2.329248439038235	5.2836845695347066
5	1.2171690107333377	3.4512941023924375
6	0.6880629742966895	2.3412107909326565
7	0.4202792209528834	1.6683879209694903
8	0.2762874642032091	1.2534650979258684
9	0.19641014335038978	1.0024610338841227
>10	0.6409212576238738	5.611881156872437
>50	0.01952741156282664	0.7642449149650772
>100	0.0078105914235014635	0.8068320198355241
>500	2.574028875912059E-4	0.09882416684170539
>1k	4.290048126520065E-4	0.47417110087268016
>5k	0.0	0.0
>10k+	5.148057751824078E-4	10.285206666009788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	112282	4.206599237895285	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	27355	1.0248438944142917	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	20997	0.7866440230676982	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	17937	0.6720023737565034	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	17726	0.66409734499681	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	15081	0.5650035010660549	No Hit
GTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGAGT	3837	0.14375163673433147	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.492918255633645E-5	0.0	0.0	0.0
6	0.0	7.492918255633645E-5	0.0	0.0	0.0
7	0.0	7.492918255633645E-5	0.0	0.0	0.0
8	0.0	7.492918255633645E-5	0.0	0.0	0.0
9	0.0	7.492918255633645E-5	0.0	0.0	0.0
10-11	0.0	7.492918255633645E-5	0.0	0.0	0.0
12-13	0.0	7.492918255633645E-5	0.0	0.0	2.4351984330809343E-4
14-15	0.0	7.492918255633645E-5	0.0	0.0	3.559136171425981E-4
16-17	0.0	7.492918255633645E-5	0.0	1.8732295639084112E-5	5.245042778943551E-4
18-19	0.0	7.492918255633645E-5	0.0	5.619688691725234E-5	5.245042778943551E-4
20-21	0.0	7.492918255633645E-5	0.0	3.37181321503514E-4	6.368980517288598E-4
22-23	0.0	7.492918255633645E-5	0.0	6.181657560897756E-4	6.74362643007028E-4
24-25	0.0	7.492918255633645E-5	0.0	0.0013112606947358878	6.74362643007028E-4
26-27	0.0	7.492918255633645E-5	0.0	0.0023977338418027664	6.930949386461121E-4
28-29	0.0	7.492918255633645E-5	0.0	0.006443909699844934	7.867564168415326E-4
30-31	0.0	7.492918255633645E-5	0.0	0.016016112771416915	8.054887124806168E-4
32-33	0.0	7.492918255633645E-5	0.0	0.04076147531064703	8.804178950369532E-4
34-35	0.0	7.492918255633645E-5	0.0	0.07633410472926774	9.92811668871458E-4
36-37	0.0	7.492918255633645E-5	0.0	0.12410145860893224	0.001011543964510542
38-39	0.0	9.366147819542055E-5	0.0	0.20026697267744822	0.001011543964510542
40-41	0.0	1.1239377383450466E-4	0.0	0.28456230305332675	0.001011543964510542
42-43	0.0	1.1239377383450466E-4	0.0	0.37655660693686877	0.001030276260149626
44-45	0.0	1.1239377383450466E-4	0.0	0.48799503369378017	0.0011426700339841308
46-47	0.0	1.1239377383450466E-4	0.0	0.6340320104960799	0.0012925283990968036
48-49	0.0	1.1239377383450466E-4	0.0	0.7657013165432021	0.001348725286014056
50-51	0.0	1.1239377383450466E-4	0.0	0.915784469203544	0.0013861898772922242
52-53	0.0	1.1239377383450466E-4	0.0	1.0630577775180234	0.0014985836511267289
54-55	0.0	2.4351984330809343E-4	0.0	1.2392724825948875	0.0016484420162394017
56-57	0.0	2.6225213894717753E-4	0.0	1.4931512853913944	0.0016484420162394017
58-59	0.0	2.997167302253458E-4	0.0	1.728878493713629	0.0016671743118784857
60-61	0.0	2.997167302253458E-4	0.0	1.9692700436499955	0.0017233711987957381
62-63	0.0	2.997167302253458E-4	0.0	2.20887483716952	0.0017983003813520746
64-65	0.0	3.37181321503514E-4	0.0	2.452694397207839	0.0017983003813520746
66-67	0.0	3.746459127816822E-4	0.0	2.7523174659549894	0.0017983003813520746
68-69	0.0	3.746459127816822E-4	0.0	3.1049341990651085	0.0017983003813520746
70-71	0.0	3.933782084207663E-4	0.0	3.4689401679237912	0.0018170326769911586
72-73	0.0	4.1211050405985043E-4	0.0	3.8111979415454966	0.001891961859547495
74-75	0.0	4.1211050405985043E-4	0.0	4.171963223258618	0.0019481587464647474
76-77	0.0	4.1211050405985043E-4	0.0	4.534302017805421	0.0019481587464647474
78-79	0.0	4.1211050405985043E-4	0.0	5.073604809254653	0.002004355633382
80-81	0.0	4.4957509533801864E-4	0.0	5.638477184251235	0.0020792848159383362
82-83	0.0	4.4957509533801864E-4	0.0	6.126341091875541	0.002210410885411925
84-85	0.0	4.4957509533801864E-4	0.0	6.610308682006918	0.002210410885411925
86-87	0.0	4.4957509533801864E-4	0.0	7.084554210701611	0.0023040723636073455
88	0.0	4.4957509533801864E-4	0.0	7.493779941233042	0.0023977338418027664
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	695	0.0	29.097143	1
GAGTACG	1475	0.0	27.101517	1
GTACGCA	1390	0.0	26.044273	3
AGTACGC	1455	0.0	24.880783	2
TAGACCG	120	1.8351956E-4	23.507051	5
TACGCAG	1525	0.0	23.42998	4
GGCGTCG	585	0.0	22.49615	8
TACGGGC	565	0.0	22.466919	4
TATTCGC	105	0.0023365517	22.380533	9
CGTTAAC	260	7.0940587E-10	21.70574	1
TAACGCC	470	0.0	21.006304	4
CGGGCGT	605	0.0	20.975994	6
CGCAGTG	1740	0.0	20.799631	6
TACCTGG	1735	0.0	20.32348	2
ACGGGCG	630	0.0	20.148901	5
GTATAAG	970	0.0	19.87828	1
TCTAACG	450	0.0	19.85077	2
GGACCGT	190	9.045592E-6	19.790216	6
TGATCGC	465	0.0	19.20394	9
TAGGCAT	1080	0.0	19.153896	5
>>END_MODULE