FastQCFastQC Report
Mon 16 Jan 2017
ERR1146614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669187
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1206774.5211144816755064No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC311651.1675839871841125No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT280361.0503572810747244No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC244340.9154098232907624No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA224600.8414547201076582No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG198240.7426980574984068No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG106720.39982211812061125No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT93950.3519798350583905No Hit
GAGTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGA84760.31754987567375387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69240.2594048300100368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66880.2505631864683891No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA59380.2224647430097629No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG57900.216919983500594No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54550.20436934542240764No Hit
GTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGAGT43220.16192196350424307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.13105114029103243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG3250.023.1537232
GTACGCA12200.023.1282633
TACGCAG12450.023.0415734
GTACTAG6750.022.993361
TAGTACT11500.022.9003334
GAGTACT74400.022.80390412-13
CTAGTAC11250.022.5731853
GAGTACG13050.022.3446461
CTGTGCG11850.021.8131949
AGTACGC13000.021.7066152
GTACTTT80200.020.97913714-15
CGCAGTG14100.020.6667376
CATGGGG42300.020.2340434
TACCTGG19900.019.852282
ACTGCGC5950.019.7468558
AGTACTT79300.019.70576912-13
GTACCTG21750.019.6777041
GGGCACC11850.019.44851194
CGTTAAC2702.579145E-819.1611351
GTACACG2753.1832315E-818.812751
Produced by FastQC (version 0.11.5)