##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146614_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2669187
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32469699575189	33.0	31.0	34.0	30.0	34.0
2	32.31766713984445	33.0	31.0	34.0	30.0	34.0
3	32.468757340718355	34.0	31.0	34.0	30.0	34.0
4	35.889739459992875	37.0	35.0	37.0	35.0	37.0
5	35.972067899326646	37.0	35.0	37.0	35.0	37.0
6	36.107489658836194	37.0	35.0	37.0	35.0	37.0
7	36.10360158355334	37.0	36.0	37.0	35.0	37.0
8	36.11219183968752	37.0	36.0	37.0	35.0	37.0
9	37.86812950909771	39.0	38.0	39.0	35.0	39.0
10-11	37.822477406041614	39.0	38.0	39.0	35.0	39.0
12-13	37.81706077543462	39.0	38.0	39.0	35.0	39.0
14-15	39.01432608505886	40.0	38.0	41.0	36.0	41.0
16-17	39.05752538132398	40.0	38.5	41.0	36.0	41.0
18-19	39.21896423892369	40.0	39.0	41.0	36.0	41.0
20-21	39.26603044297758	40.0	39.0	41.0	36.0	41.0
22-23	39.20949225363378	40.0	39.0	41.0	36.0	41.0
24-25	39.157508260005756	40.5	39.0	41.0	36.0	41.0
26-27	38.998280000614415	40.0	38.5	41.0	35.5	41.0
28-29	39.001342168982546	40.0	38.5	41.0	35.0	41.0
30-31	38.97148719816184	40.0	38.5	41.0	35.0	41.0
32-33	38.8457779466182	40.0	38.0	41.0	35.0	41.0
34-35	38.71062836736429	40.0	38.0	41.0	35.0	41.0
36-37	38.592250749010844	40.0	38.0	41.0	34.5	41.0
38-39	38.51049757847615	40.0	38.0	41.0	35.0	41.0
40-41	38.57894145295927	40.0	38.0	41.0	35.0	41.0
42-43	38.502623083358344	40.0	38.0	41.0	34.5	41.0
44-45	38.3613478561075	40.0	38.0	41.0	34.0	41.0
46-47	38.12562195904596	40.0	38.0	41.0	33.0	41.0
48-49	37.80336484480105	40.0	37.0	41.0	33.0	41.0
50-51	37.623270868620295	40.0	37.0	41.0	33.0	41.0
52-53	37.69304436144789	40.0	37.0	41.0	33.0	41.0
54-55	37.56988232746525	40.0	37.0	41.0	32.5	41.0
56-57	37.33573331505061	39.5	36.5	41.0	32.0	41.0
58-59	37.1327398567429	39.0	36.0	41.0	32.0	41.0
60-61	36.71992089726197	39.0	35.0	41.0	31.0	41.0
62-63	36.61538307357259	39.0	35.0	40.5	31.0	41.0
64-65	36.353677917658075	38.0	35.0	40.0	31.0	41.0
66-67	35.98331008655444	37.0	35.0	39.5	30.5	41.0
68-69	35.523315151767186	37.0	35.0	39.0	30.0	41.0
70-71	34.99722162591081	36.0	34.0	39.0	29.5	40.5
72-73	34.5867011191048	35.5	34.0	37.5	29.0	40.0
74-75	34.34020302811305	35.0	34.0	37.0	29.0	39.0
76-77	34.077934030099804	35.0	34.0	36.5	29.0	39.0
78-79	33.780914750446485	35.0	34.0	36.0	29.0	39.0
80-81	33.4699457175537	35.0	34.0	36.0	29.0	37.5
82-83	33.15448224496822	35.0	33.5	35.5	28.0	37.0
84-85	32.98670681372268	35.0	33.0	35.0	28.0	37.0
86-87	32.99800950626539	35.0	34.0	35.0	29.0	36.0
88-89	32.81917359105975	35.0	33.5	35.0	28.0	36.0
90-91	32.68767399961112	35.0	33.0	35.0	27.0	36.0
92-93	32.55155034098398	35.0	33.0	35.0	27.0	35.5
94-95	32.421507185521286	35.0	33.0	35.0	26.5	35.0
96-97	32.346154091114634	35.0	33.0	35.0	25.5	35.0
98-99	32.40293523833287	35.0	33.0	35.0	27.0	35.0
100	31.493803543925548	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28360819703905094
1101	2	0.35698083897977995
1101	3	0.2982546567664883
1101	4	0.23450429586073795
1101	5	0.20553134823000363
1101	6	0.22503656276270334
1101	7	0.22187587531843178
1101	8	0.14934461809260569
1101	9	0.2468159624579016
1101	10-11	0.29458755898105693
1101	12-13	0.2541531583260763
1101	14-15	0.4330390157184638
1101	16-17	0.4804011468561953
1101	18-19	0.41162732400997015
1101	20-21	0.33824936310893605
1101	22-23	0.34735715838439774
1101	24-25	0.40021610697800725
1101	26-27	0.3562754831809869
1101	28-29	0.36606749062882216
1101	30-31	0.5113773190224933
1101	32-33	0.4388629185360031
1101	34-35	0.4620870828980941
1101	36-37	0.4713785233198635
1101	38-39	0.5413700287656624
1101	40-41	0.520624422226156
1101	42-43	0.4680836441054623
1101	44-45	0.4671512213905231
1101	46-47	0.4923128561600265
1101	48-49	0.4808271128340351
1101	50-51	0.49551034516900216
1101	52-53	0.4994851743288109
1101	54-55	0.593198522594804
1101	56-57	0.5491601167374753
1101	58-59	0.5556570722647152
1101	60-61	0.5127595843495669
1101	62-63	0.5906171903128055
1101	64-65	0.5399543165388039
1101	66-67	0.4976456115426515
1101	68-69	0.41219196365184274
1101	70-71	0.39565609875894125
1101	72-73	0.4459293911971187
1101	74-75	0.4879630801587993
1101	76-77	0.48388373982751887
1101	78-79	0.45904597872183217
1101	80-81	0.38203537287280653
1101	82-83	0.4027775663194362
1101	84-85	0.4231795534131706
1101	86-87	0.4506990498762917
1101	88-89	0.388304808946792
1101	90-91	0.45309978654394456
1101	92-93	0.417706516391668
1101	94-95	0.359113566419893
1101	96-97	0.3061308884897329
1101	98-99	0.3940677283039875
1101	100	0.4717182616732529
1102	1	0.29577889537689117
1102	2	0.27111756325098924
1102	3	0.24562363958215627
1102	4	0.17511792494698852
1102	5	0.1902997818456953
1102	6	0.21718927787411246
1102	7	0.2256237284899214
1102	8	0.20709762041813207
1102	9	0.28872852387094383
1102	10-11	0.26820428985043065
1102	12-13	0.262754541999513
1102	14-15	0.36744010312147424
1102	16-17	0.3918091752671131
1102	18-19	0.3716268550229458
1102	20-21	0.3306385932263112
1102	22-23	0.27837369583706106
1102	24-25	0.2901610252610638
1102	26-27	0.3559142823154602
1102	28-29	0.36411335109063003
1102	30-31	0.4085877357093821
1102	32-33	0.37945034903697916
1102	34-35	0.3573252474446136
1102	36-37	0.36307405694623895
1102	38-39	0.30437334078434475
1102	40-41	0.3371824132798267
1102	42-43	0.39386450749439206
1102	44-45	0.38700675721628386
1102	46-47	0.46142375380118494
1102	48-49	0.4367519552382291
1102	50-51	0.43578476726379023
1102	52-53	0.44769914237947006
1102	54-55	0.41021054160010095
1102	56-57	0.46102273911883884
1102	58-59	0.3603753242692278
1102	60-61	0.34692828059799297
1102	62-63	0.3506494695573821
1102	64-65	0.32377167682109587
1102	66-67	0.3728482462793892
1102	68-69	0.34122363483047735
1102	70-71	0.30985015265039806
1102	72-73	0.27372188546416254
1102	74-75	0.330032347924508
1102	76-77	0.29326978730193787
1102	78-79	0.2629727746995485
1102	80-81	0.3412083546922844
1102	82-83	0.3853141068771109
1102	84-85	0.3401439459779425
1102	86-87	0.385796825584066
1102	88-89	0.4205864793168388
1102	90-91	0.3490327796191153
1102	92-93	0.40715641078872267
1102	94-95	0.39117028473981463
1102	96-97	0.45817939145081255
1102	98-99	0.44036135622585704
1102	100	0.5418470228682963
1103	1	0.18994630308831972
1103	2	0.17470922352060114
1103	3	0.13439757152764287
1103	4	0.1741916189981083
1103	5	0.09509263133380585
1103	6	0.1751061693876963
1103	7	0.1553698351547581
1103	8	0.1457891507198923
1103	9	0.21325564864260826
1103	10-11	0.23596520094279327
1103	12-13	0.19631940431719386
1103	14-15	0.3102819591029089
1103	16-17	0.4103880224294727
1103	18-19	0.3638403430509598
1103	20-21	0.36157547014701663
1103	22-23	0.36130214638213687
1103	24-25	0.33027323970548395
1103	26-27	0.3197460727556063
1103	28-29	0.2914361780560384
1103	30-31	0.39429206391746874
1103	32-33	0.3729191795543443
1103	34-35	0.3613430401192872
1103	36-37	0.3760727420668175
1103	38-39	0.33010928690215735
1103	40-41	0.3658670208301231
1103	42-43	0.458576241083108
1103	44-45	0.4120836801957637
1103	46-47	0.5020819345552994
1103	48-49	0.5890165436860357
1103	50-51	0.5468984118612568
1103	52-53	0.5149297947775437
1103	54-55	0.4894255345190075
1103	56-57	0.4965719862933682
1103	58-59	0.5113003318063534
1103	60-61	0.497193314939409
1103	62-63	0.4428783485150021
1103	64-65	0.4342703176056659
1103	66-67	0.4239773261424773
1103	68-69	0.37761178267461304
1103	70-71	0.380019490878432
1103	72-73	0.44731225365573124
1103	74-75	0.40138276582308663
1103	76-77	0.3722453657602145
1103	78-79	0.4049822854073284
1103	80-81	0.4242717133858136
1103	82-83	0.3481610044963972
1103	84-85	0.3416078260629618
1103	86-87	0.36047747962889787
1103	88-89	0.4879813332993592
1103	90-91	0.4552514118911688
1103	92-93	0.413037424335279
1103	94-95	0.4432308958673943
1103	96-97	0.41464412025376873
1103	98-99	0.4276795152426658
1103	100	0.32064326216114836
1104	1	0.2049583392176899
1104	2	0.19305517755248047
1104	3	0.23927864917342134
1104	4	0.1859392859675495
1104	5	0.1767439043455994
1104	6	0.16515275505405924
1104	7	0.155622923628556
1104	8	0.17295048281813763
1104	9	0.2057491059645642
1104	10-11	0.21732542595695747
1104	12-13	0.21198657011599664
1104	14-15	0.37720452467208077
1104	16-17	0.42716069846723315
1104	18-19	0.31540740062952466
1104	20-21	0.2972959816300502
1104	22-23	0.3692092313605073
1104	24-25	0.33144661242977946
1104	26-27	0.4062224771455689
1104	28-29	0.37746857218157714
1104	30-31	0.40147533234863175
1104	32-33	0.37658101827919666
1104	34-35	0.37100319558470574
1104	36-37	0.31853839488069724
1104	38-39	0.332011497472962
1104	40-41	0.27289545337533383
1104	42-43	0.3670847578062748
1104	44-45	0.3827518352654735
1104	46-47	0.3712363552472482
1104	48-49	0.5231959659518282
1104	50-51	0.45916274804548607
1104	52-53	0.413876408623139
1104	54-55	0.4670720965028252
1104	56-57	0.44263903487856027
1104	58-59	0.41858882268856945
1104	60-61	0.3697649921133248
1104	62-63	0.3304344821445184
1104	64-65	0.30217747003433715
1104	66-67	0.34856546718252446
1104	68-69	0.3676462272889367
1104	70-71	0.35761632701019863
1104	72-73	0.369351935410279
1104	74-75	0.3936772073197048
1104	76-77	0.3988968860897302
1104	78-79	0.3892925237616538
1104	80-81	0.45219527122368675
1104	82-83	0.36449457915187367
1104	84-85	0.33117600181743256
1104	86-87	0.3901472130353767
1104	88-89	0.3944866139075458
1104	90-91	0.42141385017415445
1104	92-93	0.41686683955625625
1104	94-95	0.4059370314454753
1104	96-97	0.4591168313656837
1104	98-99	0.4286858360842061
1104	100	0.4349037521800021
1105	1	0.1805560789776095
1105	2	0.1404269974623844
1105	3	0.14043845439697122
1105	4	0.14834430640866003
1105	5	0.11296763686649314
1105	6	0.12451218009897502
1105	7	0.10964630721837665
1105	8	0.10154668623975027
1105	9	0.12010371367750139
1105	10-11	0.15845450441437237
1105	12-13	0.2016295667568926
1105	14-15	0.2492619160217231
1105	16-17	0.2909119678993122
1105	18-19	0.27662553462947415
1105	20-21	0.21470243425093827
1105	22-23	0.17152415467379
1105	24-25	0.18074067799757643
1105	26-27	0.17799291174703313
1105	28-29	0.13339076576020403
1105	30-31	0.15034286873902403
1105	32-33	0.13935528070661718
1105	34-35	0.08654885607998608
1105	36-37	0.14075280521321787
1105	38-39	0.1176891789041008
1105	40-41	0.07809348477913858
1105	42-43	0.043840740411802415
1105	44-45	0.10161129936984281
1105	46-47	0.15440850325651923
1105	48-49	0.27651263392047554
1105	50-51	0.22706785962531484
1105	52-53	0.1702772106438104
1105	54-55	0.15749680290596757
1105	56-57	0.05352620515171935
1105	58-59	0.1215620279069114
1105	60-61	0.14872236523521565
1105	62-63	0.08288819669807168
1105	64-65	0.16400076508940487
1105	66-67	0.1839517651280005
1105	68-69	0.03706736626244833
1105	70-71	0.08143165393778418
1105	72-73	0.15396615825745386
1105	74-75	0.1156598649245737
1105	76-77	0.0779802910320484
1105	78-79	0.01258028773843023
1105	80-81	0.09277335106120432
1105	82-83	0.08521639775980105
1105	84-85	0.07169210733002984
1105	86-87	0.08503198766236153
1105	88-89	0.13462358237354977
1105	90-91	0.04986891878859012
1105	92-93	0.0840277384443695
1105	94-95	0.18191234587403926
1105	96-97	0.18325036260021932
1105	98-99	0.19967508171605886
1105	100	0.25300962932501747
1106	1	0.07744880548717248
1106	2	0.06828392616658618
1106	3	0.06451592636435066
1106	4	-0.013187144092029257
1106	5	0.0576429381796828
1106	6	0.06901538811001018
1106	7	0.03975124215871517
1106	8	0.08196762525755474
1106	9	0.05288898680816345
1106	10-11	0.0397628932133145
1106	12-13	0.004225057552595501
1106	14-15	0.16094986949056533
1106	16-17	0.21604928276409652
1106	18-19	0.16080044618430378
1106	20-21	0.20894510379581988
1106	22-23	0.1553686710736244
1106	24-25	0.1148709172492417
1106	26-27	0.056027030593355676
1106	28-29	0.16819648621351746
1106	30-31	0.17157180679742368
1106	32-33	0.09418948350088385
1106	34-35	0.11196259955902832
1106	36-37	0.13646546336516963
1106	38-39	0.0999269914603218
1106	40-41	0.06938208584647754
1106	42-43	0.08127762954053708
1106	44-45	0.13007325295862415
1106	46-47	0.14665141265269455
1106	48-49	0.21790317178700036
1106	50-51	0.14012686304318578
1106	52-53	0.10868849564710814
1106	54-55	0.21097944366869825
1106	56-57	0.12787860666277595
1106	58-59	0.08200935056686376
1106	60-61	0.12580007705389562
1106	62-63	0.12410394293539895
1106	64-65	0.14183250940792647
1106	66-67	0.15059384703721435
1106	68-69	0.1769084647813699
1106	70-71	0.21753556951012598
1106	72-73	0.15734146556692252
1106	74-75	0.027385594971946148
1106	76-77	0.07677893587225526
1106	78-79	0.16904547931964942
1106	80-81	0.16516916565615247
1106	82-83	0.12832397106434712
1106	84-85	0.06863940000857127
1106	86-87	0.07901082078582533
1106	88-89	0.12923772160722535
1106	90-91	0.08657126333496734
1106	92-93	0.08257393979952354
1106	94-95	0.18218697560359942
1106	96-97	0.16949489099261683
1106	98-99	0.1943114071942631
1106	100	0.10480071884951059
1107	1	0.1337009588797713
1107	2	0.09084823382269747
1107	3	0.15614445267603827
1107	4	-0.0036709631848950153
1107	5	0.06396713234292406
1107	6	0.08590740818771536
1107	7	0.11368432445708265
1107	8	0.09454076421067725
1107	9	0.13150777501058997
1107	10-11	0.1366103646433814
1107	12-13	0.13233173620822924
1107	14-15	0.2115389664038645
1107	16-17	0.24976860973166026
1107	18-19	0.20930334653198912
1107	20-21	0.16627326593109615
1107	22-23	0.18825490726294447
1107	24-25	0.17924764744369526
1107	26-27	0.21598272004440844
1107	28-29	0.20920214252174674
1107	30-31	0.19138107701092366
1107	32-33	0.1792229943005026
1107	34-35	0.17242709446001925
1107	36-37	0.1451179953728925
1107	38-39	0.1721675358226875
1107	40-41	0.12183802931190968
1107	42-43	0.11032652966463274
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2216	62-63	-0.9498020269323675
2216	64-65	-0.8335976185263405
2216	66-67	-0.9175916063189362
2216	68-69	-0.9394845003858521
2216	70-71	-0.8608271512236243
2216	72-73	-0.7651993986757049
2216	74-75	-0.7419892602992846
2216	76-77	-0.7131465094148979
2216	78-79	-0.7446332096823909
2216	80-81	-0.8380208354658691
2216	82-83	-0.8574051754596539
2216	84-85	-0.8402157924419029
2216	86-87	-0.7833918080854474
2216	88-89	-0.7474647064939077
2216	90-91	-0.8160197098869268
2216	92-93	-0.7545727727983831
2216	94-95	-0.8280371421178856
2216	96-97	-0.8330887413473675
2216	98-99	-0.8107770772896181
2216	100	-1.1305187109728507
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	15.0
8	83.0
9	171.0
10	240.0
11	140.0
12	155.0
13	178.0
14	331.0
15	575.0
16	949.0
17	1644.0
18	2800.0
19	4424.0
20	6338.0
21	9198.0
22	13423.0
23	16335.0
24	16391.0
25	16146.0
26	17065.0
27	19502.0
28	24211.0
29	29673.0
30	37014.0
31	46901.0
32	59498.0
33	78368.0
34	112157.0
35	175627.0
36	334863.0
37	686466.0
38	767268.0
39	190619.0
40	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.34877137454615	18.54988964531769	13.442178155280335	20.659160824855825
2	13.853203756620674	25.01029372811295	34.733530128683775	26.402972386582597
3	25.9879910639189	24.07421907635269	30.44130798271678	19.49648187701162
4	11.217970583803325	16.51079436025882	42.673651745157585	29.59758331078027
5	11.021406879064509	41.498878665878394	34.91717563171986	12.562538823337238
6	27.591288132964216	39.02146419942084	16.142678534564183	17.24456913305076
7	24.512332781479902	33.679581085926166	23.03225663844459	18.77582949414934
8	24.680060318263134	27.987336911217888	20.505137355175286	26.827465415343692
9	22.141806528282405	20.209307256968977	24.619490736127045	33.02939547862157
10-11	26.1871507595015	23.577477769431493	27.524618937990436	22.710752533076576
12-13	30.971359443905577	22.592085155517392	25.763312948849222	20.67324245172781
14-15	23.67689556349847	25.01537453452578	30.41391075520575	20.89381914677
16-17	20.131920590637993	28.13624701055361	29.347779459733253	22.384052939075143
18-19	19.401063472278576	32.317497389648736	27.279734869984463	21.001704268088226
20-21	22.926288862180687	25.76793362445938	25.50839659880053	25.797380914559398
22-23	21.102886384505844	26.362090778952542	27.34774671088987	25.187276125651742
24-25	25.861451445702382	26.364038937699007	26.07127188915576	21.70323772744285
26-27	23.57632199875767	22.913833397398	32.35970025228741	21.15014435155692
28-29	19.73419616800849	31.4744211391748	28.03728776539219	20.754094927424514
30-31	22.3058205141654	25.56925722506538	26.886609370668147	25.238312890101078
32-33	23.097766377708247	26.160555780239246	28.41504586887868	22.32663197317383
34-35	25.825098752220015	24.827564115652727	28.069813937274006	21.277523194853252
36-37	23.93436124990189	23.271031556255643	31.877889341149203	20.916717852693264
38-39	21.943957712586304	27.24464560613196	30.221290344576186	20.590106336705553
40-41	21.52762037351672	28.48760907412826	28.063207640124965	21.92156291223005
42-43	20.793859703347874	28.33229743738449	24.76478792980784	26.109054929459795
44-45	23.185297038243817	24.38980711841274	26.14993800522371	26.274957838119732
46-47	25.949493235206077	27.25200594787851	27.07920052060796	19.719300296307452
48-49	24.642765488341947	26.377602151967732	27.53183087721533	21.447801482474986
50-51	18.718508669493744	27.516412300824182	32.019375188025414	21.745703841656656
52-53	21.98735564359774	29.83832082946978	25.339440089166125	22.83488343776635
54-55	20.599060946677394	25.92575802845633	27.152034662363217	26.32314636250306
56-57	24.700133074377785	23.71771672541121	27.049034461468374	24.533115738742627
58-59	28.221040703935635	24.32234880989913	25.799721223626342	21.656889262538893
60-61	23.48358347966466	26.251596469465614	30.749566063770363	19.515253987099364
62-63	20.71319656224009	26.828464172517403	29.77150285855581	22.686836406686698
64-65	19.59311030690938	31.749073824482505	26.2531819282771	22.404633940331014
66-67	22.781077614047287	26.161940328703153	24.5488274196038	26.50815463764576
68-69	20.968005705258648	26.482564548980907	26.444274725363243	26.1051550203972
70-71	25.35865099046532	26.106094228801553	25.182336811036233	23.35291796969689
72-73	23.845063594279438	23.209176998457913	30.903283044372877	22.042476362889772
74-75	20.653164920236566	30.903130124013177	26.490968998485076	21.95273595726518
76-77	21.951854151855837	25.58910335125151	26.36019373734933	26.098848759543326
78-79	22.925445984342595	26.44457865269569	27.284976651760562	23.34499871120116
80-81	25.333035611591516	24.779246946322793	26.779390062562776	23.108327379522915
82-83	23.378670806783237	23.6180525167393	30.58808962193463	22.41518705454284
84-85	22.050831128685434	27.156141896464874	28.388284529487894	22.40474244536179
86-87	21.46413870590558	28.21619466901345	27.06827959225037	23.251387032830596
88-89	20.737943800865207	28.04679851954921	24.30136966799254	26.913888011593045
90-91	22.785893754520337	24.549520987012336	25.575676334343818	27.088908924123512
92-93	25.150448545155168	27.05460655593374	26.13247114756002	21.66247375135107
94-95	23.26209494401565	25.878456838954683	27.215157743931805	23.644290473097858
96-97	19.05824119503547	26.84058675786085	31.05321642334563	23.047955623758043
98-99	22.11723803333227	28.527022293321746	25.204903369380542	24.15083630396544
100	21.621996538764936	22.268653627548314	25.84037677673234	30.26897305695441
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	314.0
1	199.0
2	250.5
3	443.5
4	314.0
5	192.5
6	388.0
7	918.0
8	1714.5
9	2176.0
10	1701.0
11	1080.0
12	994.5
13	1071.0
14	1207.0
15	1402.0
16	1633.0
17	1850.5
18	2172.5
19	2542.5
20	2845.0
21	3263.0
22	3898.5
23	4707.5
24	5572.5
25	6815.5
26	8858.0
27	11083.0
28	12978.0
29	16020.5
30	20628.5
31	25154.0
32	30490.5
33	35737.5
34	39785.0
35	44020.5
36	48951.0
37	54216.0
38	56363.5
39	56318.5
40	55845.0
41	55302.0
42	55137.0
43	56668.5
44	60800.5
45	70826.5
46	94088.5
47	184442.0
48	347946.0
49	331873.0
50	176414.0
51	107329.0
52	81823.0
53	71616.5
54	64880.5
55	56813.0
56	50839.5
57	44887.5
58	38220.0
59	33784.5
60	28531.5
61	22750.0
62	17970.5
63	13949.0
64	10139.5
65	6835.5
66	4743.5
67	3598.0
68	2734.0
69	1948.5
70	1536.0
71	1242.0
72	941.5
73	809.0
74	666.5
75	460.0
76	306.5
77	183.5
78	90.0
79	43.5
80	23.5
81	12.0
82	9.0
83	6.0
84	3.5
85	2.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002472663024359103
2	0.003221954849922467
3	2.997167302253458E-4
4	0.052637750745826356
5	0.00168590660751757
6	0.005170113596387214
7	0.0
8	4.4957509533801864E-4
9	3.7464591278168224E-5
10-11	7.492918255633645E-5
12-13	0.0
14-15	1.8732295639084112E-5
16-17	2.0605525202992521E-4
18-19	2.2478754766900932E-4
20-21	4.1211050405985043E-4
22-23	0.0
24-25	0.0
26-27	3.37181321503514E-4
28-29	1.8732295639084112E-5
30-31	2.622521389471776E-4
32-33	9.366147819542055E-5
34-35	2.4351984330809345E-4
36-37	5.619688691725233E-5
38-39	8.991501906760373E-4
40-41	5.994334604506916E-4
42-43	0.0
44-45	3.933782084207663E-4
46-47	0.0
48-49	7.492918255633645E-5
50-51	0.0
52-53	4.4957509533801864E-4
54-55	3.559136171425981E-4
56-57	1.1239377383450466E-4
58-59	8.42953303758785E-4
60-61	5.994334604506916E-4
62-63	2.622521389471776E-4
64-65	4.3084279969893453E-4
66-67	0.001161402329623215
68-69	0.0026412536851108596
70-71	0.0032968840324788034
72-73	0.0029597027109752893
74-75	0.0028285766415017005
76-77	0.0041023727449594205
78-79	0.0013112606947358878
80-81	0.0010490085557887103
82-83	7.118272342851962E-4
84-85	5.807011648116075E-4
86-87	0.0
88-89	0.0
90-91	1.8732295639084112E-5
92-93	7.492918255633645E-5
94-95	1.311260694735888E-4
96-97	4.1211050405985043E-4
98-99	7.492918255633645E-5
100	0.07234412575814284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2669187.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.13953517167749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63251111230106	38.39164902918755
2	14.725673513310046	15.355795441477376
3	5.396132277985643	8.440554860971698
4	2.474431670112872	5.16062868375051
5	1.2885285607611736	3.35916401067591
6	0.7567756618553038	2.367475874302447
7	0.4681802186549913	1.7087489284071906
8	0.2997405214358163	1.2502665167823757
9	0.20776684476360494	0.9749580039054393
>10	0.7113323098126797	5.764295205684367
>50	0.02366362783626209	0.8531156748902051
>100	0.01276516035332971	1.2340066263104574
>500	8.030959769901217E-4	0.2877143855597117
>1k	4.4616443166117824E-4	0.6178601294037788
>5k	6.246302043256495E-4	2.264215441081649
>10k+	6.246302043256495E-4	11.969551187609289
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	120677	4.5211144816755064	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	31165	1.1675839871841125	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	28036	1.0503572810747244	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	24434	0.9154098232907624	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	22460	0.8414547201076582	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	19824	0.7426980574984068	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	10672	0.39982211812061125	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	9395	0.3519798350583905	No Hit
GAGTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGA	8476	0.31754987567375387	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6924	0.2594048300100368	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6688	0.2505631864683891	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	5938	0.2224647430097629	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	5790	0.216919983500594	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	5455	0.20436934542240764	No Hit
GTAAAGCAGTGGTATCAACGCAGAGTAAAGCAGTGGTATCAACGCAGAGT	4322	0.16192196350424307	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3498	0.13105114029103243	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1239377383450466E-4
2	0.0	0.0	0.0	0.0	1.1239377383450466E-4
3	0.0	0.0	0.0	0.0	1.1239377383450466E-4
4	0.0	0.0	0.0	0.0	1.1239377383450466E-4
5	0.0	0.0	0.0	0.0	1.1239377383450466E-4
6	0.0	0.0	0.0	0.0	1.498583651126729E-4
7	0.0	0.0	0.0	0.0	1.498583651126729E-4
8	0.0	0.0	0.0	0.0	1.498583651126729E-4
9	0.0	0.0	0.0	0.0	1.498583651126729E-4
10-11	0.0	0.0	0.0	0.0	1.498583651126729E-4
12-13	0.0	1.8732295639084112E-5	0.0	0.0	2.997167302253458E-4
14-15	0.0	3.7464591278168224E-5	0.0	0.0	3.933782084207663E-4
16-17	0.0	3.7464591278168224E-5	0.0	1.8732295639084112E-5	5.05771982255271E-4
18-19	0.0	3.7464591278168224E-5	0.0	5.619688691725234E-5	5.619688691725233E-4
20-21	0.0	1.1239377383450466E-4	0.0	3.37181321503514E-4	5.619688691725233E-4
22-23	0.0	1.1239377383450466E-4	0.0	5.994334604506916E-4	5.619688691725233E-4
24-25	0.0	1.498583651126729E-4	0.0	0.0013112606947358878	5.619688691725233E-4
26-27	0.0	1.873229563908411E-4	0.0	0.0023977338418027664	6.368980517288598E-4
28-29	0.0	1.873229563908411E-4	0.0	0.006575035769318524	6.368980517288598E-4
30-31	0.0	1.873229563908411E-4	0.0	0.016278364910364093	6.368980517288598E-4
32-33	0.0	1.873229563908411E-4	0.0	0.041154853519067794	6.74362643007028E-4
34-35	0.0	1.873229563908411E-4	0.0	0.07697100278099661	7.492918255633644E-4
36-37	0.0	2.0605525202992521E-4	0.0	0.12522539634727728	7.492918255633644E-4
38-39	0.0	2.2478754766900932E-4	0.0	0.20163443025910138	8.054887124806168E-4
40-41	0.0	2.2478754766900932E-4	0.0	0.28604215440881436	8.242210081197009E-4
42-43	0.0	2.2478754766900932E-4	0.0	0.37833617502258177	8.242210081197009E-4
44-45	0.0	2.2478754766900932E-4	0.0	0.49001812162280123	8.242210081197009E-4
46-47	0.0	2.2478754766900932E-4	0.0	0.6364110120422435	8.242210081197009E-4
48-49	0.0	2.2478754766900932E-4	0.0	0.7686048223672601	8.991501906760373E-4
50-51	0.0	2.2478754766900932E-4	0.0	0.9194747314444436	9.366147819542055E-4
52-53	0.0	2.2478754766900932E-4	0.0	1.0681529619318542	9.740793732323737E-4
54-55	0.0	2.4351984330809343E-4	0.0	1.2458662506598452	9.92811668871458E-4
56-57	0.0	2.6225213894717753E-4	0.0	1.5023301102545457	0.0010490085557887101
58-59	0.0	2.6225213894717753E-4	0.0	1.7398743512537713	0.0010864731470668783
60-61	0.0	2.6225213894717753E-4	0.0	1.9829633517621659	0.0010864731470668783
62-63	0.0	2.6225213894717753E-4	0.0	2.225321792740636	0.0011614023296232148
64-65	0.0	2.6225213894717753E-4	0.0	2.4703027551085777	0.0012737961034577196
66-67	0.0	2.6225213894717753E-4	0.0	2.7730166526361772	0.0013112606947358878
68-69	0.0	2.6225213894717753E-4	0.0	3.1291363250308053	0.001348725286014056
70-71	0.0	2.809844345862617E-4	0.0	3.497638044842868	0.0013861898772922242
72-73	0.0	2.997167302253458E-4	0.0	3.8439045297313377	0.0014611190598485607
74-75	0.0	2.997167302253458E-4	0.0	4.20933415305859	0.001536048242404897
76-77	0.0	2.997167302253458E-4	0.0	4.576168698558774	0.0016109774249612335
78-79	0.0	2.997167302253458E-4	0.0	5.119330342909658	0.0016297097206003177
80-81	0.0	2.997167302253458E-4	0.0	5.68764946030383	0.0017046389031566541
82-83	0.0	2.997167302253458E-4	0.0	6.1784356060478345	0.0018357649726302428
84-85	0.0	2.997167302253458E-4	0.0	6.664707268542818	0.0018357649726302428
86-87	0.0	2.997167302253458E-4	0.0	7.141350531079313	0.002004355633382
88	0.0	2.997167302253458E-4	0.0	7.551962451488038	0.0021354817028555887
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	325	0.0	23.153723	2
GTACGCA	1220	0.0	23.128263	3
TACGCAG	1245	0.0	23.041573	4
GTACTAG	675	0.0	22.99336	1
TAGTACT	1150	0.0	22.900333	4
GAGTACT	7440	0.0	22.803904	12-13
CTAGTAC	1125	0.0	22.573185	3
GAGTACG	1305	0.0	22.344646	1
CTGTGCG	1185	0.0	21.813194	9
AGTACGC	1300	0.0	21.706615	2
GTACTTT	8020	0.0	20.979137	14-15
CGCAGTG	1410	0.0	20.666737	6
CATGGGG	4230	0.0	20.234043	4
TACCTGG	1990	0.0	19.85228	2
ACTGCGC	595	0.0	19.746855	8
AGTACTT	7930	0.0	19.705769	12-13
GTACCTG	2175	0.0	19.677704	1
GGGCACC	1185	0.0	19.448511	94
CGTTAAC	270	2.579145E-8	19.161135	1
GTACACG	275	3.1832315E-8	18.81275	1
>>END_MODULE