FastQCFastQC Report
Mon 16 Jan 2017
ERR1146613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744335
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT383871.3987723801941088No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC98780.35994147944766214No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67970.2476738444832719No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG59320.21615436890904355No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG53340.19436402625772728No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51450.1874771119415086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10350.025.441071
CATGGGG49050.020.7976064
GTACCCG3102.9831426E-1019.7182961
CTGTGCG11700.019.6805159
GAGTACG7100.019.2055781
GCATCGT9700.018.8941358
CTAACGC3255.620677E-1018.8041023
ACATGGG142550.018.5996383
TAGTACT14250.018.4742074
TGTACGC2552.9862895E-718.4353922
GGCGTCG5700.018.137698
GTACATG159900.018.1142331
ATGGGGG29750.018.0140135
CGACGAG5350.018.00703224-25
TACATGG158300.017.847862
CAATGCG5100.017.50707418-19
TACCTGG23550.017.3668462
GTATAAG14750.017.2143041
TCTAACG3852.910383E-1017.0946372
CATGGGC24050.017.0057894
Produced by FastQC (version 0.11.5)