##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146613_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2744335
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139521960693575	31.0	31.0	34.0	28.0	34.0
2	31.319292651953933	31.0	31.0	34.0	28.0	34.0
3	31.51873841932563	33.0	31.0	34.0	28.0	34.0
4	35.04917766963581	37.0	35.0	37.0	32.0	37.0
5	35.19131410706054	37.0	35.0	37.0	32.0	37.0
6	35.24601770556437	37.0	35.0	37.0	32.0	37.0
7	35.164336715452016	37.0	35.0	37.0	32.0	37.0
8	35.17313884784474	37.0	35.0	37.0	32.0	37.0
9	36.82757498628994	39.0	37.0	39.0	33.0	39.0
10-11	36.828527311716684	39.0	37.0	39.0	33.0	39.0
12-13	36.79816713338569	39.0	37.0	39.0	33.0	39.0
14-15	38.11770228488869	40.0	38.0	41.0	33.0	41.0
16-17	38.15557503001638	40.0	38.0	41.0	33.0	41.0
18-19	38.174962240397036	40.0	38.0	41.0	33.0	41.0
20-21	38.250251700320845	40.0	38.0	41.0	33.0	41.0
22-23	38.242997301714254	40.0	38.0	41.0	33.0	41.0
24-25	38.230524516868385	40.0	38.0	41.0	33.0	41.0
26-27	38.24662768940381	40.0	38.0	41.0	33.0	41.0
28-29	38.23520033086339	40.0	38.0	41.0	33.0	41.0
30-31	38.22058822993549	40.0	38.0	41.0	33.0	41.0
32-33	38.1640780006814	40.0	38.0	41.0	33.0	41.0
34-35	38.08766859731046	40.0	38.0	41.0	33.0	41.0
36-37	38.009607792051625	40.0	38.0	41.0	32.0	41.0
38-39	37.94934219036671	40.0	38.0	41.0	32.0	41.0
40-41	37.937072369080305	40.0	38.0	41.0	32.0	41.0
42-43	37.792138897036985	40.0	38.0	41.0	32.0	41.0
44-45	37.673258731168026	40.0	37.5	41.0	31.0	41.0
46-47	37.5466666788129	40.0	37.0	41.0	31.0	41.0
48-49	37.42209660992554	40.0	37.0	41.0	31.0	41.0
50-51	37.288109687775	40.0	37.0	41.0	30.0	41.0
52-53	37.22393548892537	40.0	36.0	41.0	30.0	41.0
54-55	37.11365430969616	40.0	36.0	41.0	30.0	41.0
56-57	36.86777507119211	39.0	35.5	41.0	30.0	41.0
58-59	36.67110156012294	39.0	35.0	41.0	30.0	41.0
60-61	36.40663676264013	39.0	35.0	41.0	29.0	41.0
62-63	36.225047051471485	38.5	35.0	40.0	29.0	41.0
64-65	35.96359792080777	38.0	35.0	40.0	29.0	41.0
66-67	35.662405099960466	37.0	34.5	40.0	28.5	41.0
68-69	35.34155323602986	37.0	34.0	39.5	28.0	41.0
70-71	34.954983265526984	36.0	34.0	39.0	28.0	41.0
72-73	34.58602302561458	36.0	34.0	39.0	27.5	41.0
74-75	34.27916307593643	35.0	34.0	38.5	28.0	40.0
76-77	34.002156260077584	35.0	34.0	37.0	27.0	39.5
78-79	33.635416230161404	35.0	33.0	37.0	26.5	39.0
80-81	33.30986650682223	35.0	33.0	36.0	26.0	39.0
82-83	33.022345850634125	35.0	33.0	36.0	26.0	37.5
84-85	32.8196165919977	35.0	33.0	35.5	26.0	37.0
86-87	32.6751161939049	35.0	33.0	35.0	26.0	37.0
88-89	32.48358709851385	35.0	33.0	35.0	26.0	36.0
90-91	32.27151878323893	35.0	33.0	35.0	25.0	36.0
92-93	32.07308674050362	35.0	32.0	35.0	25.0	36.0
94-95	31.937029735801204	35.0	32.0	35.0	25.0	35.5
96-97	31.84061621485715	35.0	32.0	35.0	25.0	35.0
98-99	31.79449301925603	35.0	32.0	35.0	24.0	35.0
100	30.6120167545143	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16304379097330823
1101	2	0.27681254394907384
1101	3	0.17506153733418017
1101	4	0.23670586657592452
1101	5	0.22914252558500436
1101	6	0.23708054730992956
1101	7	0.32428130120089804
1101	8	0.2817930126549655
1101	9	0.38109166739392464
1101	10-11	0.3886293942439849
1101	12-13	0.3800295773816629
1101	14-15	0.4536680477461914
1101	16-17	0.47803791229149084
1101	18-19	0.480741857777069
1101	20-21	0.4589556447466805
1101	22-23	0.4658237192936667
1101	24-25	0.5488536572948561
1101	26-27	0.5018824010988752
1101	28-29	0.5410155541530344
1101	30-31	0.5831121473851155
1101	32-33	0.5515144484788692
1101	34-35	0.5121900859519499
1101	36-37	0.5389680221362667
1101	38-39	0.5656132468686081
1101	40-41	0.4676236140438732
1101	42-43	0.5132887706502771
1101	44-45	0.5155366057834527
1101	46-47	0.47834753301584243
1101	48-49	0.501656092154839
1101	50-51	0.4870347955616907
1101	52-53	0.48253067239299696
1101	54-55	0.4705017364091759
1101	56-57	0.4565404379281546
1101	58-59	0.4580445983459285
1101	60-61	0.46433248007893013
1101	62-63	0.4685923638007168
1101	64-65	0.5200730657340031
1101	66-67	0.42360205249104865
1101	68-69	0.4703545950976604
1101	70-71	0.43621463234005375
1101	72-73	0.4908745521936595
1101	74-75	0.45819965205611624
1101	76-77	0.44445208156714955
1101	78-79	0.3852886701648117
1101	80-81	0.3883017824406423
1101	82-83	0.411489124979731
1101	84-85	0.4215865839449222
1101	86-87	0.40879327317004766
1101	88-89	0.33516873725063334
1101	90-91	0.3163114435983019
1101	92-93	0.32035575281472717
1101	94-95	0.39124546352961787
1101	96-97	0.4275661432097415
1101	98-99	0.43602049140384835
1101	100	0.41569365487066534
1102	1	0.2498709225443001
1102	2	0.3417179520746707
1102	3	0.35009715722519275
1102	4	0.4114006960692578
1102	5	0.44755064921503873
1102	6	0.4643215187412366
1102	7	0.45736183657786
1102	8	0.42462484152736835
1102	9	0.4513592378518112
1102	10-11	0.5078357935631175
1102	12-13	0.5795394063734065
1102	14-15	0.6206052649426184
1102	16-17	0.6377890254745537
1102	18-19	0.5892482746029017
1102	20-21	0.6155136715653597
1102	22-23	0.6230981599810548
1102	24-25	0.6804731706350182
1102	26-27	0.6083340763975897
1102	28-29	0.6553143422571353
1102	30-31	0.7111354942320389
1102	32-33	0.5990208594035735
1102	34-35	0.5965352176160437
1102	36-37	0.6492438771283915
1102	38-39	0.6402792099891528
1102	40-41	0.64195134392412
1102	42-43	0.7268787835737598
1102	44-45	0.5617653395774767
1102	46-47	0.6045215305281886
1102	48-49	0.5730815139710614
1102	50-51	0.6136381824182919
1102	52-53	0.539483403010891
1102	54-55	0.4362227957804663
1102	56-57	0.5529201095760357
1102	58-59	0.59661392452184
1102	60-61	0.5976129163168693
1102	62-63	0.5086675278358044
1102	64-65	0.6156501833717627
1102	66-67	0.5659185157981383
1102	68-69	0.5146568550346728
1102	70-71	0.5049420891945715
1102	72-73	0.6196005436002991
1102	74-75	0.5959937989195652
1102	76-77	0.5354690991216984
1102	78-79	0.49672409767890713
1102	80-81	0.38115370794736947
1102	82-83	0.447135898928984
1102	84-85	0.42142315536076325
1102	86-87	0.3831074468377551
1102	88-89	0.3764683043042183
1102	90-91	0.3653227252521418
1102	92-93	0.34958570093066754
1102	94-95	0.41007097753464805
1102	96-97	0.45858659325633155
1102	98-99	0.4038125204164409
1102	100	0.3526908701918394
1103	1	0.15151127033104572
1103	2	0.16989832307878672
1103	3	0.25240964714688374
1103	4	0.3013366763459544
1103	5	0.322980969206661
1103	6	0.3732525643712421
1103	7	0.3414772723086159
1103	8	0.323283767032315
1103	9	0.5048923975006616
1103	10-11	0.5180434507102589
1103	12-13	0.4997355389004241
1103	14-15	0.5220252081907404
1103	16-17	0.5276518687389498
1103	18-19	0.5598098629217745
1103	20-21	0.5230381097974472
1103	22-23	0.5274758725273756
1103	24-25	0.5390780420032968
1103	26-27	0.565949802154428
1103	28-29	0.5483681984576947
1103	30-31	0.5738109714758792
1103	32-33	0.6032250148392109
1103	34-35	0.5559254903169872
1103	36-37	0.5531697374435751
1103	38-39	0.6065167730669643
1103	40-41	0.5176336694518398
1103	42-43	0.5446274627896344
1103	44-45	0.46231478424821404
1103	46-47	0.5334266610475638
1103	48-49	0.46257561524557644
1103	50-51	0.5028045045436542
1103	52-53	0.4348255611870684
1103	54-55	0.32598017501459964
1103	56-57	0.48423001104358576
1103	58-59	0.483183462383046
1103	60-61	0.460328018995277
1103	62-63	0.5054713563329472
1103	64-65	0.45204257223139876
1103	66-67	0.50460230177341
1103	68-69	0.45512099611803336
1103	70-71	0.4154983126040719
1103	72-73	0.3678384154278689
1103	74-75	0.3697544628232805
1103	76-77	0.3649334182711428
1103	78-79	0.30416878829100114
1103	80-81	0.31189735163791
1103	82-83	0.47485448268461994
1103	84-85	0.4243278487393738
1103	86-87	0.39339832886095394
1103	88-89	0.3873815387408257
1103	90-91	0.3894210268991358
1103	92-93	0.38080274819765236
1103	94-95	0.3547855865191032
1103	96-97	0.41054604615785095
1103	98-99	0.4002038546357731
1103	100	0.45049637770691575
1104	1	0.1860909902168011
1104	2	0.09956106485324767
1104	3	0.19928370658644567
1104	4	0.19368071607803472
1104	5	0.21693114414652115
1104	6	0.2443986782649077
1104	7	0.3004747655506108
1104	8	0.27326499967250584
1104	9	0.4154684186379711
1104	10-11	0.40534615664783047
1104	12-13	0.3672110729282778
1104	14-15	0.4754089807036763
1104	16-17	0.4687765211920052
1104	18-19	0.48404269885575957
1104	20-21	0.4829067944054728
1104	22-23	0.40319960857200954
1104	24-25	0.40853232225926206
1104	26-27	0.4495750176282698
1104	28-29	0.5090961124323314
1104	30-31	0.4994892098276722
1104	32-33	0.47256588411525513
1104	34-35	0.4687578041325011
1104	36-37	0.5158998305366964
1104	38-39	0.4447807186184676
1104	40-41	0.46312304852935426
1104	42-43	0.5157875381958235
1104	44-45	0.4232196726718769
1104	46-47	0.4396601256004118
1104	48-49	0.4745900229652378
1104	50-51	0.505637183182607
1104	52-53	0.45530418849676835
1104	54-55	0.4211094312588699
1104	56-57	0.49039222978750985
1104	58-59	0.4499816525781384
1104	60-61	0.42191249631903815
1104	62-63	0.39214775864059703
1104	64-65	0.36954560833418526
1104	66-67	0.39846660288745284
1104	68-69	0.3724457949214184
1104	70-71	0.35029668100138167
1104	72-73	0.35626404090714203
1104	74-75	0.36560417520910704
1104	76-77	0.36237460276866784
1104	78-79	0.3237530018193553
1104	80-81	0.28553419235108635
1104	82-83	0.3164610177950138
1104	84-85	0.28058551792449293
1104	86-87	0.3153446338659762
1104	88-89	0.29357859110772466
1104	90-91	0.18994009348432428
1104	92-93	0.24477032942524346
1104	94-95	0.28046940246007424
1104	96-97	0.3084163893093752
1104	98-99	0.2514592331506158
1104	100	0.25576681360778863
1105	1	0.08247780037076424
1105	2	0.15459881687913324
1105	3	0.11174806159959516
1105	4	0.15924536614943463
1105	5	0.16788771803553715
1105	6	0.16523441946957007
1105	7	0.1743246279198445
1105	8	0.20990462539833032
1105	9	0.2833370558531527
1105	10-11	0.31823519465896055
1105	12-13	0.31887836309412165
1105	14-15	0.42621489584277583
1105	16-17	0.3148695103926116
1105	18-19	0.3604523043336272
1105	20-21	0.4130080487305463
1105	22-23	0.3984090970164118
1105	24-25	0.4846808586859197
1105	26-27	0.44909865940689286
1105	28-29	0.3975282133722402
1105	30-31	0.47611162988755495
1105	32-33	0.5340710450872095
1105	34-35	0.4935808346878403
1105	36-37	0.41507455538432936
1105	38-39	0.44308708136689035
1105	40-41	0.4806456800937511
1105	42-43	0.4922360092642535
1105	44-45	0.434525629296715
1105	46-47	0.511347125133149
1105	48-49	0.47671816878543893
1105	50-51	0.3963422661322582
1105	52-53	0.385964408618662
1105	54-55	0.3970156763915682
1105	56-57	0.3738187483476523
1105	58-59	0.3668493188813784
1105	60-61	0.40264819137990315
1105	62-63	0.3566981914751821
1105	64-65	0.37028070658261214
1105	66-67	0.3802922084701237
1105	68-69	0.4241337551766122
1105	70-71	0.3992036408533792
1105	72-73	0.377573884091035
1105	74-75	0.3767172595212358
1105	76-77	0.3043673449471527
1105	78-79	0.3958312046278749
1105	80-81	0.38985009245341473
1105	82-83	0.33239918511502964
1105	84-85	0.29648984161227077
1105	86-87	0.2713006036643577
1105	88-89	0.23318299179886282
1105	90-91	0.3319315983687261
1105	92-93	0.4258708806761433
1105	94-95	0.38839601782027344
1105	96-97	0.5104963534424023
1105	98-99	0.3744368031923848
1105	100	0.5024352173383022
1106	1	0.11893096907972023
1106	2	0.16465262789426305
1106	3	0.17228694305130077
1106	4	0.16566279046606525
1106	5	0.18498009362218681
1106	6	0.22031635059821042
1106	7	0.30134928345957945
1106	8	0.22975612957183955
1106	9	0.33416178033587585
1106	10-11	0.29471472851677305
1106	12-13	0.3918387043522671
1106	14-15	0.4111625265315837
1106	16-17	0.40252290003684976
1106	18-19	0.3764177101013715
1106	20-21	0.36693508563464405
1106	22-23	0.40946620713447146
1106	24-25	0.429570580345775
1106	26-27	0.421254747033025
1106	28-29	0.3512451285729483
1106	30-31	0.360987870446408
1106	32-33	0.4262242550709985
1106	34-35	0.4164294302772973
1106	36-37	0.4481367569818815
1106	38-39	0.4115280507172443
1106	40-41	0.3462012940876278
1106	42-43	0.3079286539988715
1106	44-45	0.39733296672096685
1106	46-47	0.34136010847633
1106	48-49	0.36689725974129317
1106	50-51	0.309043264678003
1106	52-53	0.2852980473544946
1106	54-55	0.30301235121561376
1106	56-57	0.26956529570534116
1106	58-59	0.28382405193872984
1106	60-61	0.36001164702606303
1106	62-63	0.3757955637303141
1106	64-65	0.3524937122063818
1106	66-67	0.36062625841668705
1106	68-69	0.27844798616224864
1106	70-71	0.16131121535001114
1106	72-73	0.24010140731157037
1106	74-75	0.27806188467533133
1106	76-77	0.20964015690918103
1106	78-79	0.20092253678089378
1106	80-81	0.22807684849269805
1106	82-83	0.2878405076363961
1106	84-85	0.2983765166996051
1106	86-87	0.209719794028274
1106	88-89	0.19741774745326524
1106	90-91	0.2722054330725143
1106	92-93	0.2019156099493884
1106	94-95	0.28736806144794613
1106	96-97	0.34962333363926135
1106	98-99	0.29487172180641963
1106	100	0.3170127436305812
1107	1	-0.08959967939028957
1107	2	-0.17174050385190398
1107	3	-0.1035326690528251
1107	4	-0.13824270653049808
1107	5	-0.07897925329898925
1107	6	-0.0662856309506239
1107	7	-0.03126631626153653
1107	8	-0.013406318838093512
1107	9	0.010121956767292772
1107	10-11	0.043599963550356335
1107	12-13	0.018584084767510944
1107	14-15	-0.043004568222514195
1107	16-17	0.03218445631888045
1107	18-19	-0.00934278107383335
1107	20-21	0.0808309808352945
1107	22-23	0.09225470995062324
1107	24-25	0.12773611035341048
1107	26-27	0.06871175599651025
1107	28-29	0.06215584842583155
1107	30-31	0.08167752910496517
1107	32-33	0.09638174575448488
1107	34-35	0.06308542603522227
1107	36-37	0.07687775150703402
1107	38-39	0.09199865750701264
1107	40-41	0.06759642820884437
1107	42-43	0.11778352382680168
1107	44-45	0.08885833925813813
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2216	68-69	-1.0214141697002361
2216	70-71	-0.9590824501432778
2216	72-73	-0.9136947373645725
2216	74-75	-0.7801439008418285
2216	76-77	-0.9337028514530985
2216	78-79	-0.8206744002812698
2216	80-81	-0.8190211336978166
2216	82-83	-0.815772343751128
2216	84-85	-0.848315888123544
2216	86-87	-0.88214706979646
2216	88-89	-0.8656983655027943
2216	90-91	-0.8580850690077781
2216	92-93	-0.8478041924728963
2216	94-95	-0.827278504989966
2216	96-97	-0.7891854914655276
2216	98-99	-0.8254402757395987
2216	100	-0.8181620984451676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	762.0
1	187.0
2	140.0
3	153.0
4	113.0
5	145.0
6	192.0
7	444.0
8	1238.0
9	1541.0
10	1661.0
11	2288.0
12	3198.0
13	4301.0
14	5635.0
15	6306.0
16	6805.0
17	7541.0
18	8352.0
19	9279.0
20	10614.0
21	11861.0
22	13368.0
23	15593.0
24	18590.0
25	22476.0
26	25510.0
27	25537.0
28	28450.0
29	35165.0
30	42228.0
31	49438.0
32	63817.0
33	85752.0
34	123936.0
35	187766.0
36	322729.0
37	613821.0
38	755925.0
39	230998.0
40	480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47252493141172	19.3662231254664	13.123504133533597	23.03774780958828
2	14.250122692810018	22.123063258443924	39.50281883540641	24.123995213339644
3	20.566612135405766	25.832574198860897	30.34755838285029	23.253255282883043
4	11.730972821363931	17.213671043212234	40.91379033060952	30.141565804814306
5	12.250600455273831	39.80706659090926	35.50891547529873	12.433417478518178
6	28.92835872824954	38.40208056082653	18.724792146327818	13.94476856459611
7	26.145237216507926	33.138904732660066	23.112873132967575	17.602984917864433
8	24.580480341939566	33.97477393367262	21.12071276310583	20.324032961281983
9	24.96616127361965	17.63114329679626	21.840381852524413	35.562313577059676
10-11	24.430781158206315	25.858783184299742	29.041474745124667	20.668960912369272
12-13	26.456858777761695	24.009979241514927	27.906579889173827	21.62658209154955
14-15	22.966619977835872	25.680923352158374	27.22958615657326	24.12287051343249
16-17	21.537840479714305	28.634292235602928	27.798052341254724	22.029814943428043
18-19	21.251558266731504	30.119634406967034	27.471971255505785	21.156836070795677
20-21	23.087942162548778	27.63569144243393	26.43023404849214	22.846132346525152
22-23	22.288762869662357	27.758468510863217	26.437503156507532	23.515265462966898
24-25	23.499012140930184	27.775905865673046	26.23856158384185	22.48652040955492
26-27	23.026754672956407	26.668928629835563	28.089753872031835	22.2145628251762
28-29	21.814847743132542	29.345330478213178	26.902736772702873	21.93708500595141
30-31	22.74239642957627	27.422013224615515	26.645341033560793	23.19024931224742
32-33	22.638875061040075	27.94710966353288	27.104492221333594	22.30952305409345
34-35	23.50763292381518	27.6337225013977	27.021633455543263	21.83701111924385
36-37	23.07096452320882	26.772636065846395	28.05242343412771	22.103975976817075
38-39	22.621026564938948	28.040872222789538	27.499981764868924	21.838119447402587
40-41	22.53894338605651	28.081359124127786	27.237185365393803	22.142512124421906
42-43	22.21964290826746	27.965839752390725	26.102351270587683	23.712166068754136
44-45	22.801906345037803	27.047218436463005	26.748604395111453	23.402270823387738
46-47	23.472258336028954	27.920595980530273	26.957302479155597	21.649843204285176
48-49	22.980701582263823	27.539425296453356	27.275915255170723	22.2039578661121
50-51	21.11104586936471	27.815243154570542	28.481359359198	22.59235161686675
52-53	22.32707269854562	28.623912570920872	26.143596806171736	22.905417924361767
54-55	21.853245327381014	27.27444797368647	27.08606437281536	23.78624232611716
56-57	23.100467356187856	26.585451341196276	27.055670605433065	23.258410697182804
58-59	24.077451979138285	26.791962425861875	26.83491065491764	22.295674940082204
60-61	22.68818249476107	27.478290650787173	28.224643277692746	21.608883576759013
62-63	21.739381785925833	27.58104084175389	27.81749962796859	22.862077744351687
64-65	21.220460572424635	28.937354775138434	26.91437849458365	22.927806157853283
66-67	22.62371536470411	27.032806381562786	26.231773548554294	24.11170470517881
68-69	22.08285483925973	27.091108007168614	26.92535164185245	23.900685511719207
70-71	23.102646269196487	27.017875780858326	26.674191429903804	23.205286520041383
72-73	22.72118736195708	26.01826000861882	28.450477983436766	22.81007464598733
74-75	21.66431856170136	28.770915232042928	26.857724900581694	22.707041305674025
76-77	22.07927092039698	26.71646274130327	26.915576522665102	24.288689815634644
78-79	22.424056613495004	27.01109059694875	27.249338994293748	23.3155137952625
80-81	22.95035087965664	26.587466231058798	27.24753470543489	23.214648183849672
82-83	22.4869568960203	26.080535855176407	28.45514131564366	22.977365933159632
84-85	22.022022569696162	27.361028510212936	27.5953810124867	23.0215679076042
86-87	21.66898397555373	27.661610410528414	27.351655149422655	23.317750464495205
88-89	21.776770377470562	27.272627799324546	26.454653035030507	24.495948788174392
90-91	22.50463305578159	26.05621088437111	26.824544964804826	24.614611095042473
92-93	23.223170743049046	26.897048238766843	27.045468761026914	22.8343122571572
94-95	22.324206757846714	26.53703346755465	27.612688373185474	23.526071401413162
96-97	21.116642504809576	26.672283158729726	28.7264057186101	23.4846686178506
98-99	22.079675512915557	27.324340270083702	26.818131664209123	23.777852552791614
100	22.180581307174073	25.391424601452613	26.57322299221388	25.85477109915944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1368.0
1	900.5
2	415.5
3	398.0
4	378.5
5	336.0
6	297.0
7	269.5
8	283.0
9	317.5
10	297.0
11	269.5
12	273.0
13	301.5
14	322.5
15	378.5
16	471.5
17	558.0
18	712.0
19	931.0
20	1219.5
21	1676.0
22	2583.5
23	3860.5
24	5446.0
25	7541.0
26	10482.5
27	13956.5
28	17817.5
29	22785.5
30	27756.0
31	32858.0
32	39645.0
33	45853.5
34	51780.0
35	58032.5
36	65047.0
37	73223.5
38	77189.5
39	78381.5
40	79730.0
41	81708.5
42	84819.0
43	89936.0
44	97738.0
45	103895.5
46	108660.5
47	134827.5
48	187699.5
49	187018.5
50	142444.5
51	116958.0
52	98401.0
53	87583.5
54	80397.0
55	71429.0
56	63165.5
57	54977.5
58	47048.5
59	41418.5
60	34544.0
61	26865.0
62	20470.5
63	15405.5
64	10998.5
65	7690.0
66	5700.5
67	4070.0
68	2948.0
69	2178.5
70	1676.5
71	1308.5
72	1056.5
73	949.0
74	777.0
75	550.5
76	398.5
77	297.0
78	191.0
79	109.0
80	72.5
81	65.0
82	48.0
83	32.5
84	25.5
85	18.5
86	18.0
87	12.0
88	7.0
89	9.0
90	8.5
91	4.5
92	3.0
93	5.0
94	5.5
95	3.5
96	2.5
97	5.5
98	6.5
99	4.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09521432332422973
2	0.06241949324699791
3	0.0793270500868152
4	0.08082103679033355
5	0.08195063649299375
6	0.0867969836044069
7	0.08318955229591139
8	0.08490217119994461
9	0.06978011066433217
10-11	0.07695853458123735
12-13	0.0671200855580678
14-15	0.07528235437728995
16-17	0.07723182483188094
18-19	0.07672168303067957
20-21	0.06888736251222974
22-23	0.07231260032029617
24-25	0.07437138687514461
26-27	0.06848653681128579
28-29	0.07750511508252454
30-31	0.08204173324320828
32-33	0.0832259909959972
34-35	0.08497504860011625
36-37	0.08442846809882905
38-39	0.0865419127038062
40-41	0.0818231010426934
42-43	0.08871001535891208
44-45	0.08504792600028786
46-47	0.08133117859153492
48-49	0.0912425050148761
50-51	0.08730712540560828
52-53	0.09280936911856606
54-55	0.08466531964938684
56-57	0.09215347251702143
58-59	0.09699981962843458
60-61	0.09479527827324288
62-63	0.09587021992577437
64-65	0.09771037428010793
66-67	0.10018820588594322
68-69	0.09694516157830585
70-71	0.09913148358345464
72-73	0.09694516157830585
74-75	0.1006619089870588
76-77	0.09789256778053701
78-79	0.10452441119615499
80-81	0.09931367708388371
82-83	0.10377741784439581
84-85	0.09978738018499929
86-87	0.096526116527319
88-89	0.09563336837521659
90-91	0.09561514902517368
92-93	0.08976673766140067
94-95	0.09002180856200136
96-97	0.08781726720680967
98-99	0.101208489488346
100	0.24206228467005667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2744335.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.88665000301256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3341516216763	49.53067379552605
2	14.14557110044867	18.357174421750845
3	4.737614286043142	9.222237600832607
4	2.0564610607204794	5.337474763671748
5	1.0144188969657806	3.2911121961930334
6	0.5865891439961229	2.2837082689225903
7	0.34476248312814584	1.5659337803833866
8	0.2057858114211198	1.0682201537014517
9	0.1494421999459909	0.872712335121816
>10	0.411529610550889	3.9895746401983545
>50	0.009620664104409449	0.4115377024733524
>100	0.0033030745373011177	0.3607978942700805
>500	0.0	0.0
>1k	3.000185846613047E-4	0.23039432261974993
>5k	3.7502323082663086E-4	1.6102295222215983
>10k+	7.500464616532618E-5	1.8682186021132956
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	38387	1.3987723801941088	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	9878	0.35994147944766214	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6797	0.2476738444832719	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	5932	0.21615436890904355	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	5334	0.19436402625772728	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	5145	0.1874771119415086	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.287740017162628E-5	0.0
3	0.0	0.0	0.0	7.287740017162628E-5	0.0
4	0.0	0.0	0.0	7.287740017162628E-5	0.0
5	0.0	0.0	0.0	7.287740017162628E-5	0.0
6	0.0	0.0	0.0	7.287740017162628E-5	0.0
7	0.0	0.0	0.0	7.287740017162628E-5	0.0
8	0.0	0.0	0.0	7.287740017162628E-5	0.0
9	0.0	0.0	0.0	7.287740017162628E-5	3.643870008581314E-5
10-11	0.0	0.0	0.0	1.8219350042906568E-4	5.465805012871971E-5
12-13	0.0	0.0	0.0	2.1863220051487883E-4	7.287740017162628E-5
14-15	0.0	0.0	0.0	2.915096006865051E-4	9.109675021453284E-5
16-17	0.0	0.0	0.0	4.0082570094394454E-4	1.4575480034325256E-4
18-19	0.0	0.0	0.0	4.190450509868511E-4	2.1863220051487883E-4
20-21	0.0	0.0	0.0	5.283611512442905E-4	2.368515505577854E-4
22-23	0.0	0.0	0.0	8.563094520166088E-4	3.2794830077231824E-4
24-25	0.0	0.0	0.0	0.0014211093033467126	3.8260635090103795E-4
26-27	0.0	0.0	0.0	0.002459612255792387	4.0082570094394454E-4
28-29	0.0	0.0	0.0	0.00621279836463114	4.0082570094394454E-4
30-31	0.0	1.821935004290657E-5	0.0	0.01641563438865882	4.0082570094394454E-4
32-33	0.0	3.643870008581314E-5	0.0	0.03844282859053286	5.10141801201384E-4
34-35	0.0	3.643870008581314E-5	0.0	0.07502728347668924	5.10141801201384E-4
36-37	0.0	3.643870008581314E-5	0.0	0.12853751455270584	5.10141801201384E-4
38-39	0.0	3.643870008581314E-5	0.0	0.21542559490732727	5.10141801201384E-4
40-41	0.0	7.287740017162628E-5	0.0	0.31067635693164286	5.10141801201384E-4
42-43	0.0	1.0931610025743942E-4	0.0	0.4076761765600774	5.10141801201384E-4
44-45	0.0	1.0931610025743942E-4	0.0	0.5253185197871251	5.283611512442905E-4
46-47	0.0	1.0931610025743942E-4	0.0	0.6754459641406753	5.830192013730102E-4
48-49	0.0	1.0931610025743942E-4	0.0	0.8289257689021202	6.558966015446365E-4
50-51	0.0	1.0931610025743942E-4	0.0	0.9928999192882793	6.558966015446365E-4
52-53	0.0	1.0931610025743942E-4	0.0	1.164016054891258	7.652127018020759E-4
54-55	0.0	1.0931610025743942E-4	0.0	1.353551953387615	9.109675021453285E-4
56-57	0.0	1.0931610025743942E-4	0.0	1.5905492587457435	9.838449023169549E-4
58-59	0.0	1.0931610025743942E-4	0.0	1.8398446253828342	0.001020283602402768
60-61	0.0	1.0931610025743942E-4	0.0	2.0965188287873016	0.001020283602402768
62-63	0.0	1.0931610025743942E-4	0.0	2.364397932468157	0.001020283602402768
64-65	0.0	1.0931610025743942E-4	0.0	2.649348567139216	0.0010385029524456746
66-67	0.0	1.0931610025743942E-4	0.0	2.9677499284890514	0.0010931610025743943
68-69	0.0	1.0931610025743942E-4	0.0	3.343123197423055	0.0011295997026602074
70-71	0.0	1.0931610025743942E-4	0.0	3.7437667048665704	0.0011295997026602074
72-73	0.0	1.0931610025743942E-4	0.0	4.126026888116794	0.0012571351529605534
74-75	0.0	1.0931610025743942E-4	0.0	4.519109365292502	0.0012753545030034599
76-77	0.0	1.0931610025743942E-4	0.0	4.934273694720215	0.0012935738530463663
78-79	0.0	1.0931610025743942E-4	0.0	5.4450531731731004	0.001311793203089273
80-81	0.0	1.0931610025743942E-4	0.0	5.987060617599528	0.0014028899533038057
82-83	0.0	1.0931610025743942E-4	0.0	6.5183004261505975	0.0015304254036041518
84-85	0.0	1.0931610025743942E-4	0.0	7.042252494684504	0.0015304254036041518
86-87	0.0	1.0931610025743942E-4	0.0	7.566532511519185	0.0015486447536470585
88	0.0	1.0931610025743942E-4	0.0	8.004270615650057	0.0015668641036899649
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1035	0.0	25.44107	1
CATGGGG	4905	0.0	20.797606	4
GTACCCG	310	2.9831426E-10	19.718296	1
CTGTGCG	1170	0.0	19.680515	9
GAGTACG	710	0.0	19.205578	1
GCATCGT	970	0.0	18.894135	8
CTAACGC	325	5.620677E-10	18.804102	3
ACATGGG	14255	0.0	18.599638	3
TAGTACT	1425	0.0	18.474207	4
TGTACGC	255	2.9862895E-7	18.435392	2
GGCGTCG	570	0.0	18.13769	8
GTACATG	15990	0.0	18.114233	1
ATGGGGG	2975	0.0	18.014013	5
CGACGAG	535	0.0	18.007032	24-25
TACATGG	15830	0.0	17.84786	2
CAATGCG	510	0.0	17.507074	18-19
TACCTGG	2355	0.0	17.366846	2
GTATAAG	1475	0.0	17.214304	1
TCTAACG	385	2.910383E-10	17.094637	2
CATGGGC	2405	0.0	17.005789	4
>>END_MODULE