FastQCFastQC Report
Mon 16 Jan 2017
ERR1146613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744335
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT396171.443591981299659No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC108080.3938294705274684No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC77930.2839667897687418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.22992819754148092No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG62850.22901723003933555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.21834069091419234No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49380.17993430102374527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33700.12279841928919029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9050.024.941891
GAGTACT71900.021.53783812-13
CATGGGG47300.020.4791154
GGCGTCG5100.020.2738388
TACCTGG21700.020.1532042
AGTACTT78050.019.26871112-13
GTACTTT80900.019.17083414-15
GTCCTAC9350.018.6091631
ACATGGG136700.018.3342763
TACGGGC5600.017.6334114
CGACGAG4550.017.55953624-25
GCATCGT9650.017.5330968
TACATGG156050.017.296932
GTACATG156800.017.2748051
GTACCTG27000.017.2428151
CTAACGC4650.017.1909963
TAGGCCG1650.001572207417.0906545
CTAGTAC13800.017.0371133
TCTAACG5000.016.9286922
TAGTACT15400.016.7937264
Produced by FastQC (version 0.11.5)