##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2744335
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38883736861571	33.0	31.0	34.0	30.0	34.0
2	32.42226295259143	34.0	31.0	34.0	30.0	34.0
3	32.55326809591395	34.0	31.0	34.0	31.0	34.0
4	36.019403243408696	37.0	35.0	37.0	35.0	37.0
5	36.06676225752322	37.0	35.0	37.0	35.0	37.0
6	36.15546498514212	37.0	36.0	37.0	35.0	37.0
7	36.13032519717891	37.0	36.0	37.0	35.0	37.0
8	36.13308433554941	37.0	36.0	37.0	35.0	37.0
9	37.91601644842922	39.0	38.0	39.0	35.0	39.0
10-11	37.87060034580327	39.0	38.0	39.0	35.0	39.0
12-13	37.85653191028064	39.0	38.0	39.0	35.0	39.0
14-15	39.180389602581315	40.0	39.0	41.0	36.0	41.0
16-17	39.14681042948474	40.0	39.0	41.0	36.0	41.0
18-19	39.26341372317884	40.5	39.0	41.0	36.0	41.0
20-21	39.3213570865073	41.0	39.0	41.0	36.0	41.0
22-23	39.28373012041168	41.0	39.0	41.0	36.0	41.0
24-25	39.270631318698335	41.0	39.0	41.0	36.0	41.0
26-27	39.18298841067144	40.5	39.0	41.0	36.0	41.0
28-29	39.14298363720172	40.0	39.0	41.0	36.0	41.0
30-31	39.05379736803269	40.0	39.0	41.0	35.0	41.0
32-33	38.958002940603095	40.0	38.0	41.0	35.0	41.0
34-35	38.88361679605441	40.0	38.0	41.0	35.0	41.0
36-37	38.82175044227472	40.0	38.0	41.0	35.0	41.0
38-39	38.75717250262814	40.0	38.0	41.0	35.0	41.0
40-41	38.71985435451576	40.0	38.0	41.0	35.0	41.0
42-43	38.63724891458222	40.0	38.0	41.0	35.0	41.0
44-45	38.52262752178579	40.0	38.0	41.0	34.5	41.0
46-47	38.32448589549016	40.0	38.0	41.0	34.0	41.0
48-49	38.07274658523832	40.0	38.0	41.0	33.0	41.0
50-51	37.96321312813487	40.0	38.0	41.0	33.0	41.0
52-53	37.93411117811783	40.0	37.5	41.0	33.0	41.0
54-55	37.798854731656306	40.0	37.0	41.0	33.0	41.0
56-57	37.62679902417162	40.0	37.0	41.0	33.0	41.0
58-59	37.46599467630592	40.0	36.0	41.0	33.0	41.0
60-61	37.21591551322998	39.0	36.0	41.0	32.0	41.0
62-63	37.02152452233419	39.0	35.0	41.0	32.0	41.0
64-65	36.76531564112982	39.0	35.0	41.0	32.0	41.0
66-67	36.45653974460115	38.0	35.0	40.0	31.5	41.0
68-69	36.110418188741534	37.0	35.0	40.0	31.0	41.0
70-71	35.72346233240475	37.0	35.0	39.0	31.0	41.0
72-73	35.36002966110187	36.0	35.0	39.0	30.5	41.0
74-75	35.049421991119885	36.0	35.0	38.5	31.0	40.5
76-77	34.73879045378935	35.0	34.0	37.0	30.0	39.5
78-79	34.42264920281234	35.0	34.0	37.0	30.0	39.0
80-81	34.117652363869574	35.0	34.0	36.5	30.0	39.0
82-83	33.83928911740003	35.0	34.0	36.0	29.5	37.5
84-85	33.631967671585286	35.0	34.0	36.0	29.0	37.0
86-87	33.49424614706295	35.0	34.0	35.0	29.0	37.0
88-89	33.29359189020291	35.0	34.0	35.0	29.0	36.0
90-91	33.18532686424945	35.0	34.0	35.0	29.0	36.0
92-93	33.06958625677987	35.0	34.0	35.0	29.0	36.0
94-95	32.97400772864829	35.0	34.0	35.0	29.0	36.0
96-97	32.91347685322674	35.0	34.0	35.0	29.0	35.0
98-99	32.87985541123806	35.0	34.0	35.0	29.0	35.0
100	32.10226266108183	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2923628590251539
1101	2	0.24020484229382078
1101	3	0.29444872327941596
1101	4	0.1731456925606878
1101	5	0.12220532628053604
1101	6	0.18737760210604648
1101	7	0.21729782365891026
1101	8	0.20807513796034982
1101	9	0.23402232455834593
1101	10-11	0.23916834694555433
1101	12-13	0.254047874376802
1101	14-15	0.42506469979952755
1101	16-17	0.42668990907414894
1101	18-19	0.3344834951091542
1101	20-21	0.3952781907795284
1101	22-23	0.3610390627938713
1101	24-25	0.3661237053960136
1101	26-27	0.27739570597336893
1101	28-29	0.35060207353335926
1101	30-31	0.404494290914883
1101	32-33	0.3485755598631002
1101	34-35	0.35445599707572484
1101	36-37	0.37301348691314473
1101	38-39	0.3438157008169256
1101	40-41	0.3742125238282199
1101	42-43	0.3623916278812729
1101	44-45	0.3731819496581039
1101	46-47	0.38689332274498867
1101	48-49	0.47823059762287556
1101	50-51	0.3625467657336543
1101	52-53	0.3023051267253294
1101	54-55	0.346247435294849
1101	56-57	0.332402259616444
1101	58-59	0.3534602716089026
1101	60-61	0.37762270155663913
1101	62-63	0.351854352376364
1101	64-65	0.2980276738430021
1101	66-67	0.27212762584034067
1101	68-69	0.28397194174653606
1101	70-71	0.3043610231445939
1101	72-73	0.3689602240392773
1101	74-75	0.30716204625093724
1101	76-77	0.2807386952846329
1101	78-79	0.25106640762210475
1101	80-81	0.24660839122217482
1101	82-83	0.2026051764522876
1101	84-85	0.18526171965238802
1101	86-87	0.24595439782479644
1101	88-89	0.33495392171904825
1101	90-91	0.3442422353456749
1101	92-93	0.2869711332714431
1101	94-95	0.27380727248537085
1101	96-97	0.2563089678107815
1101	98-99	0.22414434758451307
1101	100	0.24899360573296292
1102	1	0.2902373039089028
1102	2	0.3196601481003398
1102	3	0.2647540609848349
1102	4	0.18962362134188737
1102	5	0.24172096133090548
1102	6	0.2549988425109646
1102	7	0.24885494891569238
1102	8	0.21416690974913166
1102	9	0.2988931454910997
1102	10-11	0.2866015674361222
1102	12-13	0.3123516364798604
1102	14-15	0.44573849765689744
1102	16-17	0.5412633457166365
1102	18-19	0.409110035386675
1102	20-21	0.3907010429804174
1102	22-23	0.4263353389826037
1102	24-25	0.36759956222405066
1102	26-27	0.3844357733511856
1102	28-29	0.45247381438709766
1102	30-31	0.47576202135901724
1102	32-33	0.4621452302717515
1102	34-35	0.4351484916876629
1102	36-37	0.4634611271486335
1102	38-39	0.4685215457574614
1102	40-41	0.39560044066945466
1102	42-43	0.39070427774544925
1102	44-45	0.3599109585104543
1102	46-47	0.43840922531335735
1102	48-49	0.5299886105411176
1102	50-51	0.5389256308934449
1102	52-53	0.5109367131267604
1102	54-55	0.4766512333948114
1102	56-57	0.5191500701323903
1102	58-59	0.5155303659155521
1102	60-61	0.44680462756824113
1102	62-63	0.4729666535491788
1102	64-65	0.49072882541621254
1102	66-67	0.4381127936718272
1102	68-69	0.4238193548540039
1102	70-71	0.42338694616367434
1102	72-73	0.437783574295743
1102	74-75	0.43501786650507057
1102	76-77	0.37315370750325627
1102	78-79	0.3084768858039695
1102	80-81	0.3922020320307311
1102	82-83	0.3432284245535939
1102	84-85	0.35850388704653113
1102	86-87	0.3509813594431819
1102	88-89	0.4141435126459925
1102	90-91	0.3764067674416012
1102	92-93	0.33969480094327054
1102	94-95	0.36594214483404386
1102	96-97	0.33060611015461916
1102	98-99	0.28445129826612003
1102	100	0.40904952879793655
1103	1	0.2505801537151342
1103	2	0.21217548814923504
1103	3	0.2095959910377232
1103	4	0.14139568453165907
1103	5	0.104003737908414
1103	6	0.17522264024012912
1103	7	0.18217508650803893
1103	8	0.19699591358023127
1103	9	0.2264973619124646
1103	10-11	0.2447909258097738
1103	12-13	0.2180633925858828
1103	14-15	0.3574766060143091
1103	16-17	0.4131192375258266
1103	18-19	0.30157539465600536
1103	20-21	0.25621059855652106
1103	22-23	0.28002875810354055
1103	24-25	0.291641845657864
1103	26-27	0.31337605854732686
1103	28-29	0.3418951607653611
1103	30-31	0.3492727813649168
1103	32-33	0.3073264793785526
1103	34-35	0.306687341808761
1103	36-37	0.2709555310590517
1103	38-39	0.27635538335660925
1103	40-41	0.29591259127209213
1103	42-43	0.28627318358670095
1103	44-45	0.3262103479654286
1103	46-47	0.3976584207680034
1103	48-49	0.5036180175810685
1103	50-51	0.46878735073664757
1103	52-53	0.39897628965047005
1103	54-55	0.3572768414369847
1103	56-57	0.38343070227461595
1103	58-59	0.45934092653330083
1103	60-61	0.4144154011010528
1103	62-63	0.38604583640164236
1103	64-65	0.3552839315183718
1103	66-67	0.37738805708352885
1103	68-69	0.3154934282034745
1103	70-71	0.3405123558876042
1103	72-73	0.3495480486607292
1103	74-75	0.39417722647596776
1103	76-77	0.37048600249990926
1103	78-79	0.4121918180586164
1103	80-81	0.29996724158699806
1103	82-83	0.295117873794446
1103	84-85	0.3014109342600264
1103	86-87	0.36670788051343806
1103	88-89	0.43951640059504626
1103	90-91	0.32226191641719737
1103	92-93	0.41137342911608243
1103	94-95	0.3375013617406921
1103	96-97	0.32297605504584226
1103	98-99	0.31456609357037735
1103	100	0.42743886378608664
1104	1	0.19068386179463914
1104	2	0.16423102269626355
1104	3	0.20294989012334952
1104	4	0.09750188182957942
1104	5	0.0820733588498399
1104	6	0.1509016152546323
1104	7	0.11848371872180508
1104	8	0.14414716867810995
1104	9	0.22467071899674096
1104	10-11	0.2437328676667434
1104	12-13	0.24721527630261875
1104	14-15	0.32805398120785156
1104	16-17	0.40046165309200177
1104	18-19	0.2815370110203048
1104	20-21	0.2471355101745374
1104	22-23	0.23775863019784538
1104	24-25	0.2588176281164465
1104	26-27	0.2438132783962459
1104	28-29	0.27008668184759443
1104	30-31	0.2700969385701484
1104	32-33	0.22956643217600714
1104	34-35	0.259740668472368
1104	36-37	0.20808354269411922
1104	38-39	0.16669769999951
1104	40-41	0.32087907311827024
1104	42-43	0.3047538412791724
1104	44-45	0.2686342980631906
1104	46-47	0.2456585568752132
1104	48-49	0.3129053349851816
1104	50-51	0.27545730549152836
1104	52-53	0.2847081149533537
1104	54-55	0.30671687253116175
1104	56-57	0.31795282215139054
1104	58-59	0.30691449984562524
1104	60-61	0.23608275648889787
1104	62-63	0.2223540125861092
1104	64-65	0.26078603030555314
1104	66-67	0.20262991047080448
1104	68-69	0.10607316071772743
1104	70-71	0.15200218959125777
1104	72-73	0.14210772009511885
1104	74-75	0.1531507606399245
1104	76-77	0.24106299182250268
1104	78-79	0.29139144453553456
1104	80-81	0.25976036564731686
1104	82-83	0.23216579966793915
1104	84-85	0.18194507787558933
1104	86-87	0.20230752747659864
1104	88-89	0.26837053225561647
1104	90-91	0.23549588681176914
1104	92-93	0.1830535533244273
1104	94-95	0.22475874538130824
1104	96-97	0.2921186918914671
1104	98-99	0.2702190328195542
1104	100	0.34041689650373286
1105	1	0.1962429513208761
1105	2	0.21973967429317298
1105	3	0.1970225236528549
1105	4	0.10992358658791801
1105	5	0.13737458173308426
1105	6	0.13722223745343598
1105	7	0.16187416652037
1105	8	0.1279124146885664
1105	9	0.1597957512027719
1105	10-11	0.17085697408567313
1105	12-13	0.21409473329064355
1105	14-15	0.27456175505378866
1105	16-17	0.32666251978012184
1105	18-19	0.2551480756318014
1105	20-21	0.24696119727305188
1105	22-23	0.23827974935217355
1105	24-25	0.2574020181231944
1105	26-27	0.2818076683593205
1105	28-29	0.2245877393701292
1105	30-31	0.23797787594413933
1105	32-33	0.26807357136285503
1105	34-35	0.23390160113009273
1105	36-37	0.2541545517292789
1105	38-39	0.2027862931670228
1105	40-41	0.17254716388616487
1105	42-43	0.17878383911027385
1105	44-45	0.22722679598526696
1105	46-47	0.33235466143675296
1105	48-49	0.3444552423336873
1105	50-51	0.35754230613928684
1105	52-53	0.24737531341387609
1105	54-55	0.23231983504988563
1105	56-57	0.18099091770833553
1105	58-59	0.17563650606702907
1105	60-61	0.2013031476776206
1105	62-63	0.164080008717022
1105	64-65	0.12730965607212852
1105	66-67	0.13860867559308332
1105	68-69	0.12164086763332449
1105	70-71	0.15362813957837318
1105	72-73	0.1738643283372454
1105	74-75	0.11556358599368366
1105	76-77	0.09352731772285239
1105	78-79	0.17056640228558706
1105	80-81	0.06300258561491745
1105	82-83	0.09674068725613694
1105	84-85	0.1014787490144613
1105	86-87	0.10774659947692555
1105	88-89	0.16443746549850857
1105	90-91	0.13408071351053508
1105	92-93	0.1908619707088448
1105	94-95	0.18652672217258015
1105	96-97	0.2761565065098779
1105	98-99	0.23639323218110775
1105	100	0.2618057809448331
1106	1	0.11679471810166575
1106	2	0.11896242258127643
1106	3	0.1319413619027685
1106	4	0.08193921938640614
1106	5	0.07297727644142782
1106	6	0.08752025473094704
1106	7	0.11902785280136641
1106	8	0.1444732079902451
1106	9	0.16154033789434408
1106	10-11	0.14608823768393364
1106	12-13	0.10677725137632166
1106	14-15	0.24614529306893473
1106	16-17	0.2828078140693364
1106	18-19	0.1739788944126559
1106	20-21	0.17087837062877753
1106	22-23	0.1366989422785636
1106	24-25	0.16290144762355396
1106	26-27	0.22500699935230983
1106	28-29	0.15988719899220172
1106	30-31	0.2003666675912399
1106	32-33	0.24175236869285044
1106	34-35	0.24417539931882715
1106	36-37	0.23209007524398118
1106	38-39	0.2756467305238175
1106	40-41	0.26688594708034685
1106	42-43	0.3071653491434958
1106	44-45	0.38152307053387347
1106	46-47	0.33410667926172977
1106	48-49	0.4081283644558411
1106	50-51	0.32204109124506886
1106	52-53	0.30655301622119424
1106	54-55	0.27679337480174837
1106	56-57	0.289997925459339
1106	58-59	0.23005748272167637
1106	60-61	0.2301798274420861
1106	62-63	0.2545560759511005
1106	64-65	0.2905543285100052
1106	66-67	0.27397263232825964
1106	68-69	0.21016020853536332
1106	70-71	0.21759130011885475
1106	72-73	0.17725289314258674
1106	74-75	0.25478295567854303
1106	76-77	0.20559816256136543
1106	78-79	0.1704228725061796
1106	80-81	0.2586454622553447
1106	82-83	0.23818379158392844
1106	84-85	0.24838437701538396
1106	86-87	0.2209970726831756
1106	88-89	0.2809245496441122
1106	90-91	0.1927009211581847
1106	92-93	0.2521251954837851
1106	94-95	0.2576597413184487
1106	96-97	0.2290716921014706
1106	98-99	0.19041008330110287
1106	100	0.16839560115047902
1107	1	0.06163108633799652
1107	2	0.05185309770580915
1107	3	0.022195602243129997
1107	4	0.06639626487159234
1107	5	0.01636749896223222
1107	6	0.05417341215856908
1107	7	0.07412321804556399
1107	8	0.05084754685579185
1107	9	0.09563660834602672
1107	10-11	0.040921086441237264
1107	12-13	0.04278683573279807
1107	14-15	0.12477773346557797
1107	16-17	0.1318140762145319
1107	18-19	0.06770509764797339
1107	20-21	0.04840486055314841
1107	22-23	0.06254563604710484
1107	24-25	0.07174314979778273
1107	26-27	0.05930449301567364
1107	28-29	0.14157159637714045
1107	30-31	0.11413740961241814
1107	32-33	0.10985682784117756
1107	34-35	0.03723464548307476
1107	36-37	-0.010512921330182223
1107	38-39	0.023853786592717086
1107	40-41	0.04466578894516715
1107	42-43	0.07334901312460573
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2216	38-39	-0.6505404601113085
2216	40-41	-0.6330225963686829
2216	42-43	-0.5598207128280919
2216	44-45	-0.6345464335573681
2216	46-47	-0.6796186852434403
2216	48-49	-0.5431996083416522
2216	50-51	-0.6180290729272357
2216	52-53	-0.7090436607740358
2216	54-55	-0.6395847831177903
2216	56-57	-0.7053059510106081
2216	58-59	-0.6603876610095796
2216	60-61	-0.7214466159558697
2216	62-63	-0.748080735185539
2216	64-65	-0.6722432746392144
2216	66-67	-0.7594459205696111
2216	68-69	-0.6600699285638072
2216	70-71	-0.6097918711635231
2216	72-73	-0.6442821408667925
2216	74-75	-0.6799307151508316
2216	76-77	-0.6822667408476448
2216	78-79	-0.685707512465072
2216	80-81	-0.7292428491027181
2216	82-83	-0.6691592781857167
2216	84-85	-0.7060747939678507
2216	86-87	-0.6168169049616594
2216	88-89	-0.5300626623199634
2216	90-91	-0.6507560613935581
2216	92-93	-0.6177402030341455
2216	94-95	-0.6241189750231158
2216	96-97	-0.5478886923509876
2216	98-99	-0.5919548472022171
2216	100	-0.7214661966627816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	13.0
8	98.0
9	155.0
10	205.0
11	138.0
12	123.0
13	157.0
14	284.0
15	489.0
16	950.0
17	1646.0
18	2823.0
19	4283.0
20	5977.0
21	8893.0
22	12700.0
23	15262.0
24	14988.0
25	13972.0
26	14403.0
27	16863.0
28	20835.0
29	26620.0
30	33048.0
31	41838.0
32	53995.0
33	70570.0
34	100546.0
35	154948.0
36	283133.0
37	639156.0
38	906401.0
39	298000.0
40	823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11189391162266	19.26524936613565	13.396638387218788	23.22621833502291
2	14.357860598165237	22.3467827893121	38.8265539451029	24.468802667419766
3	20.378029925668642	25.76447537525512	30.77192162904429	23.085573070031952
4	11.686293781858668	17.534143597423817	40.29440308220202	30.485159538515493
5	11.913975030909826	39.697726866095316	35.451720728950804	12.936577374044056
6	28.981621337978257	38.421843769529715	18.191358913204684	14.405175979287337
7	25.528853266402706	33.42814686550544	23.127651371881118	17.91534849621074
8	24.921000581563984	33.49236460417939	20.88430520594507	20.702329608311555
9	25.147367212822047	16.739100729320583	21.815266722174954	36.298265335682416
10-11	24.47327801446764	25.58684590639908	28.890290970626005	21.04958510850728
12-13	26.85109507403433	23.73279865614074	28.039397522532784	21.37670874729215
14-15	23.05187286753856	25.252315269876906	27.795135760600616	23.900676101983922
16-17	21.131920503642608	28.24359123384349	28.32597937490685	22.298508887607053
18-19	20.816803803914325	29.152223255868186	28.91803138907078	21.11294155114671
20-21	22.576216676526386	26.692903788373883	28.171570443137224	22.5593090919625
22-23	21.73293347933106	26.802248996569293	28.263823476361306	23.20099404773834
24-25	22.887001769098887	26.96318051549829	28.004434589800443	22.14538312560238
26-27	22.540984502592618	25.76860290125459	29.882047712920823	21.808364883231974
28-29	21.32775335372686	28.4095964960546	28.714934583423673	21.54771556679487
30-31	22.23539078755106	26.43566917972693	28.495406893485843	22.83353313923617
32-33	22.189042670745383	27.002753862285022	28.83205448329327	21.976148983676328
34-35	23.03910244030419	26.700050649885398	28.75304719184646	21.507799717963945
36-37	22.609537545188577	25.856920424744374	29.761421081188033	21.772120948879017
38-39	22.199383088311656	27.09747241114814	29.159936086054262	21.543208414485946
40-41	22.1133368569704	27.127650949314102	28.85162773266344	21.90738446105206
42-43	21.94981662224182	27.091335423700098	27.593369614132385	23.3654783399257
44-45	22.5388926248903	26.20898412483828	28.103990116307564	23.14813313396386
46-47	23.28341838733245	27.17911625220682	28.11203807115385	21.42542728930688
48-49	22.913819132007667	26.93315643546173	28.110692106242208	22.042332326288392
50-51	21.01472670063968	27.315506306628016	29.182953247325855	22.486813745406447
52-53	22.193010408576104	28.153459313379052	26.876077109713925	22.77745316833092
54-55	21.783688806423733	26.85317039824744	27.713673749892504	23.649467045436324
56-57	22.95815102579589	26.108243079145836	27.736690069443114	23.196915825615154
58-59	23.8569222710606	26.36046368626022	27.52215718873124	22.26045685394794
60-61	22.48451163458462	27.103928917858646	28.920791978189115	21.490767469367615
62-63	21.621073562777358	27.17075336616831	28.400981004649772	22.807192066404564
64-65	21.077140560972904	28.47443348018809	27.587310743293013	22.86111521554599
66-67	22.479185045663723	26.600307327804057	26.96386074268092	23.9566468838513
68-69	21.925726371208537	26.683223661915168	27.629107758818595	23.761942208057697
70-71	22.906185973673878	26.63998039524712	27.319751515223288	23.13408211585571
72-73	22.55055347595288	25.64573712830202	29.039363249852645	22.764346145892453
74-75	21.584180784784227	28.35681418380017	27.359221938903328	22.699783092512273
76-77	21.9629012777226	26.319915852181598	27.435570454757887	24.281612415337918
78-79	22.217161230797487	26.74505211161162	27.691085019068602	23.34670163852229
80-81	22.74057630599503	26.333051051887068	27.645568679294506	23.280803962823388
82-83	22.212084099613858	25.778599921219016	28.837065834595638	23.17225014457149
84-85	21.79955489844014	27.018411681592962	27.91947360318185	23.262559816785046
86-87	21.487154447252248	27.296521743883307	27.648246296461622	23.568077512402823
88-89	21.466129317302734	26.997851938629942	26.77920151876502	24.756817225302306
90-91	22.166055984085027	25.797793890574887	27.146117627467653	24.890032497872433
92-93	22.850428237259237	26.707032295478882	27.277353337233496	23.16518613002839
94-95	21.915646852430182	26.31886854776837	27.881070490208636	23.88441410959281
96-97	20.661092127474085	26.41092655837643	29.017890572263283	23.910090741886204
98-99	21.587336233005207	27.083005764423312	27.037457364420174	24.292200638151304
100	21.55353039287686	25.158274582790312	26.995397438580852	26.29279758575197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	113.0
2	181.5
3	308.0
4	205.0
5	148.5
6	303.0
7	713.5
8	1299.0
9	1607.0
10	1301.0
11	964.0
12	1010.0
13	1151.0
14	1299.5
15	1489.0
16	1728.5
17	1987.5
18	2263.5
19	2598.5
20	2996.0
21	3563.5
22	4410.0
23	5577.0
24	7129.5
25	9005.5
26	11682.5
27	15020.5
28	18538.5
29	22976.0
30	27931.5
31	32831.5
32	39154.5
33	45277.0
34	50633.5
35	56798.5
36	63599.0
37	71429.5
38	75311.0
39	76302.0
40	78312.5
41	80386.0
42	82963.5
43	88381.5
44	96432.0
45	102451.0
46	106461.0
47	134464.5
48	191771.5
49	188430.5
50	140982.0
51	116439.5
52	97333.0
53	86433.5
54	79643.0
55	71162.0
56	63158.0
57	54952.5
58	47044.5
59	41738.0
60	34495.0
61	26453.0
62	20174.5
63	15141.0
64	10660.5
65	7262.0
66	5267.5
67	3765.5
68	2692.5
69	1932.5
70	1472.0
71	1194.5
72	983.5
73	869.5
74	698.0
75	493.5
76	368.5
77	252.0
78	130.0
79	63.0
80	32.5
81	29.5
82	23.0
83	5.5
84	4.0
85	4.0
86	1.0
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002077005904891349
2	0.004008257009439445
3	7.287740017162628E-5
4	0.04959307081679168
5	0.0022591994053204143
6	0.004372644010297576
7	3.643870008581314E-5
8	4.0082570094394454E-4
9	0.0
10-11	1.0931610025743942E-4
12-13	0.0
14-15	1.821935004290657E-5
16-17	2.915096006865051E-4
18-19	1.4575480034325256E-4
20-21	1.6397415038615912E-4
22-23	0.0
24-25	0.0
26-27	1.821935004290657E-4
28-29	0.0
30-31	1.821935004290657E-4
32-33	2.732902506435985E-4
34-35	1.821935004290657E-4
36-37	7.287740017162628E-5
38-39	7.287740017162628E-4
40-41	4.190450509868511E-4
42-43	0.0
44-45	6.012385514159168E-4
46-47	0.0
48-49	7.287740017162628E-5
50-51	0.0
52-53	4.190450509868511E-4
54-55	4.0082570094394454E-4
56-57	9.109675021453285E-5
58-59	8.198707519307955E-4
60-61	6.194579014588233E-4
62-63	2.0041285047197227E-4
64-65	4.919224511584773E-4
66-67	0.0012389158029176466
68-69	0.0028057799066076115
70-71	0.003734966758795847
72-73	0.00318838625750865
74-75	0.0028239992566505183
76-77	0.00424510855999723
78-79	0.0012024771028318335
80-81	0.001056722302488581
82-83	6.558966015446365E-4
84-85	6.376772515017299E-4
86-87	0.0
88-89	0.0
90-91	5.465805012871971E-5
92-93	1.27535450300346E-4
94-95	1.27535450300346E-4
96-97	4.5548375107266426E-4
98-99	5.465805012871971E-5
100	0.07101902646724981
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2744335.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.883366245299165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37216789123204	47.39655638206523
2	14.613601276497649	18.37904882465546
3	5.028342671254073	9.485973414100075
4	2.112676257141172	5.314087793422247
5	1.0625158452945698	3.340728652054603
6	0.5918768157217775	2.233152394508039
7	0.3562102248949985	1.5679788622674802
8	0.22957854668550695	1.1549337466630598
9	0.1522625689921815	0.8617304602246934
>10	0.4611229274653732	4.334864210168843
>50	0.011575074977692532	0.48458838591295217
>100	0.006533877912028914	0.757867126987836
>500	6.144087723818839E-4	0.27175661110630095
>1k	4.6080657928635176E-4	0.7074268419201545
>5k	3.072043861909012E-4	1.2740251290178644
>10k+	1.536021930954506E-4	2.4352811649251636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	39617	1.443591981299659	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	10808	0.3938294705274684	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	7793	0.2839667897687418	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6310	0.22992819754148092	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	6285	0.22901723003933555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5992	0.21834069091419234	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4938	0.17993430102374527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3370	0.12279841928919029	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.643870008581314E-5	0.0
3	0.0	3.643870008581314E-5	0.0	3.643870008581314E-5	0.0
4	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	0.0
5	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	0.0
6	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	7.287740017162628E-5
7	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	7.287740017162628E-5
8	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	7.287740017162628E-5
9	0.0	3.643870008581314E-5	0.0	1.0931610025743942E-4	1.4575480034325256E-4
10-11	0.0	3.643870008581314E-5	0.0	1.4575480034325256E-4	1.4575480034325256E-4
12-13	0.0	5.465805012871971E-5	0.0	1.8219350042906568E-4	1.8219350042906568E-4
14-15	0.0	7.287740017162628E-5	0.0	2.1863220051487883E-4	2.1863220051487883E-4
16-17	0.0	7.287740017162628E-5	0.0	3.2794830077231824E-4	2.1863220051487883E-4
18-19	0.0	7.287740017162628E-5	0.0	3.8260635090103795E-4	2.368515505577854E-4
20-21	0.0	7.287740017162628E-5	0.0	4.919224511584773E-4	2.732902506435986E-4
22-23	0.0	7.287740017162628E-5	0.0	8.380901019737022E-4	4.190450509868511E-4
24-25	0.0	7.287740017162628E-5	0.0	0.001348231903175086	4.919224511584774E-4
26-27	0.0	7.287740017162628E-5	0.0	0.0023867348556207606	5.465805012871972E-4
28-29	0.0	7.287740017162628E-5	0.0	0.0061034822643737	6.012385514159169E-4
30-31	0.0	7.287740017162628E-5	0.0	0.016324537638444287	6.194579014588234E-4
32-33	0.0	7.287740017162628E-5	0.0	0.03846104794057577	7.287740017162627E-4
34-35	0.0	1.0931610025743942E-4	0.0	0.07500906412664635	7.287740017162627E-4
36-37	0.0	1.0931610025743942E-4	0.0	0.12886546285347816	7.287740017162627E-4
38-39	0.0	1.0931610025743942E-4	0.0	0.21569888515797087	7.652127018020759E-4
40-41	0.0	1.0931610025743942E-4	0.0	0.3110954019826297	7.834320518449824E-4
42-43	0.0	1.0931610025743942E-4	0.0	0.40871467951252305	8.016514018878891E-4
44-45	0.0	1.0931610025743942E-4	0.0	0.5257557841881548	8.380901019737022E-4
46-47	1.821935004290657E-5	1.0931610025743942E-4	0.0	0.6757921317914906	8.745288020595153E-4
48-49	3.643870008581314E-5	1.0931610025743942E-4	0.0	0.8296363235537936	9.109675021453285E-4
50-51	3.643870008581314E-5	1.0931610025743942E-4	0.0	0.9952137767437284	9.109675021453285E-4
52-53	3.643870008581314E-5	1.0931610025743942E-4	0.0	1.1674048539992383	9.656255522740481E-4
54-55	3.643870008581314E-5	1.0931610025743942E-4	0.0	1.3579245973979126	9.838449023169547E-4
56-57	3.643870008581314E-5	1.0931610025743942E-4	0.0	1.5966345216600741	9.838449023169547E-4
58-59	3.643870008581314E-5	1.0931610025743942E-4	0.0	1.8478064813515842	9.838449023169547E-4
60-61	3.643870008581314E-5	1.0931610025743942E-4	0.0	2.105810697309184	0.001020283602402768
62-63	3.643870008581314E-5	1.0931610025743942E-4	0.0	2.375165568343515	0.001056722302488581
64-65	3.643870008581314E-5	1.4575480034325256E-4	0.0	2.6624664991701086	0.001056722302488581
66-67	3.643870008581314E-5	1.4575480034325256E-4	0.0	2.984311317678053	0.0011113803526173007
68-69	3.643870008581314E-5	1.4575480034325256E-4	0.0	3.3625632439188364	0.0011660384027460204
70-71	3.643870008581314E-5	1.4575480034325256E-4	0.0	3.7683081693743654	0.0011660384027460204
72-73	3.643870008581314E-5	1.4575480034325256E-4	0.0	4.1540118097826975	0.0014211093033467124
74-75	3.643870008581314E-5	1.4575480034325256E-4	0.0	4.55099322786759	0.0014211093033467124
76-77	3.643870008581314E-5	1.4575480034325256E-4	0.0	4.969892524054097	0.0014211093033467124
78-79	3.643870008581314E-5	1.4575480034325256E-4	0.0	5.483732853314191	0.0014211093033467124
80-81	3.643870008581314E-5	1.4575480034325256E-4	0.0	6.027106748993836	0.001475767353475432
82-83	3.643870008581314E-5	1.4575480034325256E-4	0.0	6.560678634350398	0.0016761802039474043
84-85	3.643870008581314E-5	1.4575480034325256E-4	0.0	7.085323038185936	0.0016761802039474043
86-87	3.643870008581314E-5	1.4575480034325256E-4	0.0	7.610441145122589	0.0017126189040332176
88	3.643870008581314E-5	1.4575480034325256E-4	0.0	8.047705546152347	0.0017490576041190307
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	905	0.0	24.94189	1
GAGTACT	7190	0.0	21.537838	12-13
CATGGGG	4730	0.0	20.479115	4
GGCGTCG	510	0.0	20.273838	8
TACCTGG	2170	0.0	20.153204	2
AGTACTT	7805	0.0	19.268711	12-13
GTACTTT	8090	0.0	19.170834	14-15
GTCCTAC	935	0.0	18.609163	1
ACATGGG	13670	0.0	18.334276	3
TACGGGC	560	0.0	17.633411	4
CGACGAG	455	0.0	17.559536	24-25
GCATCGT	965	0.0	17.533096	8
TACATGG	15605	0.0	17.29693	2
GTACATG	15680	0.0	17.274805	1
GTACCTG	2700	0.0	17.242815	1
CTAACGC	465	0.0	17.190996	3
TAGGCCG	165	0.0015722074	17.090654	5
CTAGTAC	1380	0.0	17.037113	3
TCTAACG	500	0.0	16.928692	2
TAGTACT	1540	0.0	16.793726	4
>>END_MODULE