FastQCFastQC Report
Mon 16 Jan 2017
ERR1146612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643146
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT761952.0914616103774044No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC191450.5255073499662105No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA121180.3326246052175784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12050.023.8054281
TGATCGC8850.022.300629
CTAACGC5100.022.1235543
GTGATCG9100.021.6894558
CATGGGG65850.021.2749394
CTAGTAC16850.021.2044733
TAGTACT19000.020.784214
GGCGTCG7750.020.6166428
TGCGCGA9300.019.73392794
GTATAAG20900.019.3501971
CGGGCGT8050.019.265346
TAGGCAT20050.019.226025
ATGGGGG46500.019.2083745
GCATCGT14200.019.1946588
ATACGGG8350.019.1428343
TACGGGC8250.018.8047624
GTACCCG4850.018.4223521
CGTTAAC3601.10958354E-1018.2876851
CTGTGCG18400.018.1322389
CTAGGCA20800.017.8554824
Produced by FastQC (version 0.11.5)