##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146612_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3643146
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321404906638385	31.0	31.0	34.0	28.0	34.0
2	31.365263977891637	31.0	31.0	34.0	28.0	34.0
3	31.53918948074	33.0	31.0	34.0	28.0	34.0
4	34.966032654195025	37.0	35.0	37.0	32.0	37.0
5	35.058510693779496	37.0	35.0	37.0	32.0	37.0
6	35.089745236671824	37.0	35.0	37.0	32.0	37.0
7	35.05835121622905	37.0	35.0	37.0	32.0	37.0
8	35.06289536570865	37.0	35.0	37.0	32.0	37.0
9	36.65325600456309	39.0	37.0	39.0	32.0	39.0
10-11	36.64802439979073	39.0	37.0	39.0	32.0	39.0
12-13	36.6101718130429	39.0	37.0	39.0	32.0	39.0
14-15	37.8842936846341	40.0	38.0	41.0	32.0	41.0
16-17	37.935159749293604	40.0	38.0	41.0	32.0	41.0
18-19	37.96772432397714	40.0	38.0	41.0	32.0	41.0
20-21	38.046843716941346	40.0	38.0	41.0	32.0	41.0
22-23	38.03678633796175	40.0	38.0	41.0	32.0	41.0
24-25	38.00826359964712	40.0	38.0	41.0	32.0	41.0
26-27	38.01540907226885	40.0	38.0	41.0	32.0	41.0
28-29	38.01364274174024	40.0	38.0	41.0	32.0	41.0
30-31	38.0072809324688	40.0	38.0	41.0	32.0	41.0
32-33	37.933027526209486	40.0	38.0	41.0	32.0	41.0
34-35	37.82851016676246	40.0	38.0	41.0	31.5	41.0
36-37	37.73195241145977	40.0	38.0	41.0	31.0	41.0
38-39	37.66253768034551	40.0	38.0	41.0	31.0	41.0
40-41	37.66275809424053	40.0	38.0	41.0	31.0	41.0
42-43	37.48735694369647	40.0	37.0	41.0	30.5	41.0
44-45	37.33018591623833	40.0	37.0	41.0	30.0	41.0
46-47	37.16613196396741	40.0	36.5	41.0	30.0	41.0
48-49	37.00310843979352	40.0	36.0	41.0	30.0	41.0
50-51	36.83671955502195	39.5	36.0	41.0	30.0	41.0
52-53	36.780985993973346	39.0	35.5	41.0	30.0	41.0
54-55	36.65951295940377	39.0	35.0	41.0	29.5	41.0
56-57	36.36860683047015	39.0	35.0	41.0	29.0	41.0
58-59	36.145031519461476	39.0	35.0	40.0	28.5	41.0
60-61	35.8322908277626	38.0	35.0	40.0	28.0	41.0
62-63	35.63724745041785	38.0	34.0	40.0	28.0	41.0
64-65	35.36791347368456	37.0	34.0	40.0	28.0	41.0
66-67	35.04839512333571	36.5	34.0	39.5	27.5	41.0
68-69	34.70622602003873	36.0	34.0	39.0	27.5	41.0
70-71	34.29414124495697	35.5	33.5	39.0	26.5	41.0
72-73	33.927961712212465	35.0	33.0	37.5	26.0	40.0
74-75	33.64978551504661	35.0	33.0	37.0	26.0	39.5
76-77	33.39339337484691	35.0	33.0	37.0	26.0	39.0
78-79	33.04409307230618	35.0	33.0	36.0	26.0	39.0
80-81	32.736563536020796	35.0	32.0	36.0	25.5	38.0
82-83	32.46529798695963	35.0	32.0	35.5	25.0	37.0
84-85	32.28358965026381	35.0	32.0	35.0	25.0	37.0
86-87	32.18869090066662	35.0	32.0	35.0	25.0	36.5
88-89	32.00575656314625	35.0	32.0	35.0	25.0	36.0
90-91	31.78026450216379	35.0	32.0	35.0	24.0	36.0
92-93	31.56260934917239	35.0	31.0	35.0	23.5	36.0
94-95	31.42922600411842	35.0	31.0	35.0	23.0	35.0
96-97	31.331030790421245	35.0	31.0	35.0	23.0	35.0
98-99	31.296532035773478	35.0	31.0	35.0	23.0	35.0
100	30.017592487372177	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31150299898594724
1101	2	0.2827450801224707
1101	3	0.25594881503122835
1101	4	0.34295459210388657
1101	5	0.2031018351972449
1101	6	0.2862718611538497
1101	7	0.3165454568099335
1101	8	0.31743749366787455
1101	9	0.3941091719497152
1101	10-11	0.38300743137634186
1101	12-13	0.39021408355714726
1101	14-15	0.47753327584842253
1101	16-17	0.443240855133908
1101	18-19	0.39137432648820436
1101	20-21	0.464686443323842
1101	22-23	0.49412936485808956
1101	24-25	0.5179714020208763
1101	26-27	0.5079162619352076
1101	28-29	0.4995164280960225
1101	30-31	0.5598830301509921
1101	32-33	0.5372445084224253
1101	34-35	0.4898834116617081
1101	36-37	0.5334975566743694
1101	38-39	0.5408787557109065
1101	40-41	0.49481761333761654
1101	42-43	0.5244762752802004
1101	44-45	0.44047762801580603
1101	46-47	0.463194248874494
1101	48-49	0.5165912870861433
1101	50-51	0.4471925601674229
1101	52-53	0.4570172220851205
1101	54-55	0.45250910582500836
1101	56-57	0.5207348663885867
1101	58-59	0.5203142543774675
1101	60-61	0.5270160432783726
1101	62-63	0.5317242094027819
1101	64-65	0.5310777235896182
1101	66-67	0.5630824842434095
1101	68-69	0.4585810493459874
1101	70-71	0.45171059171944705
1101	72-73	0.39257134511653646
1101	74-75	0.40205826971553904
1101	76-77	0.40154276713708725
1101	78-79	0.44059779057710813
1101	80-81	0.35275187143828646
1101	82-83	0.4025692271426209
1101	84-85	0.4038867810387998
1101	86-87	0.3913737514721234
1101	88-89	0.37621112567789794
1101	90-91	0.3079649800832769
1101	92-93	0.4093713437660149
1101	94-95	0.3849112584973149
1101	96-97	0.3347751049922145
1101	98-99	0.3965446689189136
1101	100	0.3134492551555397
1102	1	0.29416020254572217
1102	2	0.3770428265372203
1102	3	0.28728953023832204
1102	4	0.3199680767602118
1102	5	0.29678686441038593
1102	6	0.3675025528386726
1102	7	0.3229864397589992
1102	8	0.39802078093224935
1102	9	0.43203720681037083
1102	10-11	0.48349427191269
1102	12-13	0.4539193289150205
1102	14-15	0.5592289930608629
1102	16-17	0.524519919229391
1102	18-19	0.5437871206520768
1102	20-21	0.5343643941763929
1102	22-23	0.5219948543272679
1102	24-25	0.643512034845827
1102	26-27	0.545025353238465
1102	28-29	0.53130916112368
1102	30-31	0.5725015899810231
1102	32-33	0.5608852030339833
1102	34-35	0.5265548478209681
1102	36-37	0.5681075422574864
1102	38-39	0.5258062106446317
1102	40-41	0.5033761425995351
1102	42-43	0.5403421549805856
1102	44-45	0.48530069610031745
1102	46-47	0.4994461864808599
1102	48-49	0.4812547668645806
1102	50-51	0.49682238250382227
1102	52-53	0.48334373721199597
1102	54-55	0.4870497169704393
1102	56-57	0.5112365302911073
1102	58-59	0.5580818608509759
1102	60-61	0.5829666627704242
1102	62-63	0.5158510128671736
1102	64-65	0.5668630097049814
1102	66-67	0.5500556721072201
1102	68-69	0.5865229590941752
1102	70-71	0.5324384542279788
1102	72-73	0.5868709884885703
1102	74-75	0.5234059494657828
1102	76-77	0.5058935741569144
1102	78-79	0.40947251551830277
1102	80-81	0.29938897267076214
1102	82-83	0.363394891348662
1102	84-85	0.3677580101587168
1102	86-87	0.3376432494830368
1102	88-89	0.41506474019450934
1102	90-91	0.38431669764259624
1102	92-93	0.4469088764207818
1102	94-95	0.4304253610197293
1102	96-97	0.43364871885843215
1102	98-99	0.4287059536467801
1102	100	0.44732864119176696
1103	1	0.25968037392163623
1103	2	0.26580106921604596
1103	3	0.3000715166192016
1103	4	0.274432586866844
1103	5	0.20668825145427405
1103	6	0.27278564622179147
1103	7	0.263566149903923
1103	8	0.3167111673657814
1103	9	0.38046227173455804
1103	10-11	0.3970020927339064
1103	12-13	0.4251253031368236
1103	14-15	0.4628327341300533
1103	16-17	0.4349137726742569
1103	18-19	0.45410886935658823
1103	20-21	0.36759001918387213
1103	22-23	0.41608585589502667
1103	24-25	0.47881553297289514
1103	26-27	0.4592428522511369
1103	28-29	0.45370022611967187
1103	30-31	0.4973804181144388
1103	32-33	0.49405637549894266
1103	34-35	0.48107022019258494
1103	36-37	0.4503389595793834
1103	38-39	0.4763683882676233
1103	40-41	0.4127303115101313
1103	42-43	0.45597930956596855
1103	44-45	0.4217886866219871
1103	46-47	0.46968218739039
1103	48-49	0.37616225738026543
1103	50-51	0.42458151078443507
1103	52-53	0.4467890273662647
1103	54-55	0.4184942651340151
1103	56-57	0.4305495306860436
1103	58-59	0.415058368468344
1103	60-61	0.4469008290481469
1103	62-63	0.4312272739521461
1103	64-65	0.4231184699187125
1103	66-67	0.4384441162006141
1103	68-69	0.4474092789400359
1103	70-71	0.4477261551339211
1103	72-73	0.4508527259435482
1103	74-75	0.42749617314007793
1103	76-77	0.34740047347356295
1103	78-79	0.39615497940609146
1103	80-81	0.3552189849973857
1103	82-83	0.37119596603839966
1103	84-85	0.40527521357187624
1103	86-87	0.3847090951729015
1103	88-89	0.30047026204134397
1103	90-91	0.4258020552940778
1103	92-93	0.33811030295772326
1103	94-95	0.2329032508939477
1103	96-97	0.33622982921159306
1103	98-99	0.33013877357042887
1103	100	0.39813346173772857
1104	1	0.15590417115427257
1104	2	0.1515714023603998
1104	3	0.16367833869633586
1104	4	0.16445619345795137
1104	5	0.13393155332362028
1104	6	0.1991596109251006
1104	7	0.1381233522730696
1104	8	0.20035230426287853
1104	9	0.33373023905979693
1104	10-11	0.32882296996341864
1104	12-13	0.2557881043354513
1104	14-15	0.3300784005899331
1104	16-17	0.3275775793357667
1104	18-19	0.33180812276189897
1104	20-21	0.2878937351538724
1104	22-23	0.2736400387631406
1104	24-25	0.3710369105749862
1104	26-27	0.378577061528631
1104	28-29	0.30691560649112404
1104	30-31	0.32940713839705893
1104	32-33	0.3199937222858438
1104	34-35	0.33862997258464844
1104	36-37	0.3130152411555187
1104	38-39	0.30940063286276853
1104	40-41	0.3048943557773569
1104	42-43	0.3363376420898092
1104	44-45	0.31179333226020134
1104	46-47	0.2807635661620864
1104	48-49	0.22712615769168565
1104	50-51	0.2253663345196557
1104	52-53	0.2290505972741883
1104	54-55	0.24157078089224626
1104	56-57	0.3385186553747488
1104	58-59	0.25043282312594783
1104	60-61	0.20764130790598756
1104	62-63	0.1751509028377285
1104	64-65	0.2544321682775603
1104	66-67	0.2474671053936106
1104	68-69	0.22670395114979414
1104	70-71	0.27731495963725195
1104	72-73	0.17799392533397906
1104	74-75	0.16980054483962448
1104	76-77	0.15644813647674027
1104	78-79	0.1557226844231394
1104	80-81	0.12899282563135728
1104	82-83	0.1891147545162397
1104	84-85	0.17480073599732293
1104	86-87	0.19674501254148424
1104	88-89	0.2147263349929318
1104	90-91	0.22911110195374818
1104	92-93	0.2288217457576458
1104	94-95	0.2565767991442911
1104	96-97	0.21826077987527626
1104	98-99	0.28891739580452125
1104	100	0.3768975221238158
1105	1	0.19240287943600265
1105	2	0.1996781973385353
1105	3	0.18328769980967863
1105	4	0.21312908193340974
1105	5	0.14114964643870564
1105	6	0.1768929866167781
1105	7	0.14978101320773618
1105	8	0.26373930788363253
1105	9	0.23439499980451473
1105	10-11	0.28149754212971345
1105	12-13	0.2435930326869311
1105	14-15	0.31904215540537706
1105	16-17	0.30814400052720714
1105	18-19	0.2731984257811888
1105	20-21	0.34650688858186385
1105	22-23	0.32299545144881137
1105	24-25	0.3241943159617193
1105	26-27	0.36014062001785874
1105	28-29	0.2874932754585018
1105	30-31	0.33452298590609075
1105	32-33	0.37130302069427046
1105	34-35	0.3202560526671405
1105	36-37	0.32932310313319846
1105	38-39	0.35370434282612706
1105	40-41	0.27045381862483
1105	42-43	0.3428076530748214
1105	44-45	0.2845967065471555
1105	46-47	0.3025657279807987
1105	48-49	0.2712487956491856
1105	50-51	0.33590510336903634
1105	52-53	0.32703908245427726
1105	54-55	0.2406793230376465
1105	56-57	0.24688468619006443
1105	58-59	0.2712218830616564
1105	60-61	0.35189708974022693
1105	62-63	0.2662645334846019
1105	64-65	0.3196041882108318
1105	66-67	0.28026248822168753
1105	68-69	0.32669751948436954
1105	70-71	0.3328801567498374
1105	72-73	0.23903039203670318
1105	74-75	0.24909696418175997
1105	76-77	0.21704970190744177
1105	78-79	0.2787902344024076
1105	80-81	0.15373149721134638
1105	82-83	0.15671382679815338
1105	84-85	0.18005424940913883
1105	86-87	0.14273775652292642
1105	88-89	0.21454742087957612
1105	90-91	0.12802711043774195
1105	92-93	0.18198251210968053
1105	94-95	0.19270402145847143
1105	96-97	0.31883843051011596
1105	98-99	0.24157755019352223
1105	100	0.43099485583184816
1106	1	0.14255243784418425
1106	2	0.13173929775922488
1106	3	0.18556866543976014
1106	4	0.19959415735596053
1106	5	0.1691434416688864
1106	6	0.20185745823390988
1106	7	0.22142424098788638
1106	8	0.2534751268540276
1106	9	0.26270773077734333
1106	10-11	0.2613060981661306
1106	12-13	0.3083140951741683
1106	14-15	0.40111947463051223
1106	16-17	0.36926656598132723
1106	18-19	0.32123365381312396
1106	20-21	0.34107787929669087
1106	22-23	0.37147370258703205
1106	24-25	0.44167646012417805
1106	26-27	0.39825362382588736
1106	28-29	0.38755942861002524
1106	30-31	0.3532319719927486
1106	32-33	0.3494787608241836
1106	34-35	0.38130548380042484
1106	36-37	0.4106455469134218
1106	38-39	0.4098349888376873
1106	40-41	0.3424191952380937
1106	42-43	0.3649194480305695
1106	44-45	0.36762015312562113
1106	46-47	0.44738409745276186
1106	48-49	0.43982165258538686
1106	50-51	0.41594393575824995
1106	52-53	0.448801738616865
1106	54-55	0.36272911264958907
1106	56-57	0.41681905370524674
1106	58-59	0.287092614881729
1106	60-61	0.3611888179820042
1106	62-63	0.36113555747682824
1106	64-65	0.4184416993772331
1106	66-67	0.38877793745445643
1106	68-69	0.369948857426202
1106	70-71	0.31583033026038265
1106	72-73	0.3437252941254414
1106	74-75	0.31428777684610054
1106	76-77	0.29909103817042393
1106	78-79	0.2868059093182751
1106	80-81	0.3246074280308804
1106	82-83	0.37422179617535534
1106	84-85	0.3018476672618462
1106	86-87	0.28178214763168796
1106	88-89	0.22907853129954958
1106	90-91	0.2985305783482062
1106	92-93	0.31855976090241356
1106	94-95	0.28526381104778054
1106	96-97	0.3622212860873226
1106	98-99	0.3568706851847274
1106	100	0.22031579909882026
1107	1	0.16777760807532616
1107	2	0.15750575041508696
1107	3	0.1455764382086393
1107	4	0.14131825465323544
1107	5	0.11954611585262853
1107	6	0.2084636390298158
1107	7	0.17416041001274607
1107	8	0.11294207042086413
1107	9	0.20524153871035367
1107	10-11	0.2105796636561692
1107	12-13	0.2729931951079294
1107	14-15	0.28283310106767345
1107	16-17	0.2723802163826363
1107	18-19	0.24825524293362378
1107	20-21	0.284607537834475
1107	22-23	0.2984645398408574
1107	24-25	0.2837624380236221
1107	26-27	0.2393867010506412
1107	28-29	0.2737446101547434
1107	30-31	0.3244814906206983
1107	32-33	0.2805439282963391
1107	34-35	0.26470853316755694
1107	36-37	0.34914411508385257
1107	38-39	0.32871345887690495
1107	40-41	0.347562883113838
1107	42-43	0.34236917678388323
1107	44-45	0.3134234492441763
1107	46-47	0.3057648291439463
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2216	70-71	-0.9310411250866366
2216	72-73	-0.8324807422240923
2216	74-75	-0.749365262648837
2216	76-77	-0.7515486134766505
2216	78-79	-0.6235824230725839
2216	80-81	-0.7191810360367654
2216	82-83	-0.7339843006908282
2216	84-85	-0.7217434727297452
2216	86-87	-0.7228905468997908
2216	88-89	-0.6496851826547285
2216	90-91	-0.6115546134278489
2216	92-93	-0.603575688354713
2216	94-95	-0.7384468758599354
2216	96-97	-0.7450205789029631
2216	98-99	-0.7322063523715592
2216	100	-0.6781222742582358
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1043.0
1	268.0
2	218.0
3	199.0
4	148.0
5	246.0
6	323.0
7	947.0
8	2696.0
9	3872.0
10	4213.0
11	4797.0
12	5683.0
13	7232.0
14	9240.0
15	10220.0
16	10691.0
17	11632.0
18	12797.0
19	14151.0
20	15531.0
21	17101.0
22	19717.0
23	22753.0
24	26881.0
25	32499.0
26	35857.0
27	36929.0
28	41662.0
29	50342.0
30	60979.0
31	72649.0
32	92602.0
33	125442.0
34	183483.0
35	281933.0
36	489871.0
37	849592.0
38	850048.0
39	236219.0
40	440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10105877905213	19.202184597259528	12.9697136878569	22.72704293583144
2	14.172092083030293	22.67562199111865	37.59558107802272	25.556704847828332
3	21.79773645029256	25.543389281103206	30.246215971211164	22.412658297393072
4	11.860629811810249	16.857742656984577	40.68918665744389	30.592440873761284
5	12.066594015120568	40.12186669604228	35.2117063672478	12.59983292158935
6	28.918794250544032	37.750125208555005	17.890633520946984	15.44044701995397
7	25.40896799710556	33.2210829606077	23.41966941908097	17.950279623205773
8	24.872281623278866	31.89036384215558	21.02946670915704	22.207887825408513
9	24.377555292098524	18.290892090172626	22.63935234985238	34.69220026787647
10-11	24.893943335537383	25.216680296604295	28.102770370667457	21.78660599719087
12-13	27.579445575046346	23.63222425086599	26.89824610363516	21.890084070452506
14-15	23.62643022669361	25.342688524013745	27.33713573377373	23.693745515518913
16-17	21.559869173964234	28.210215550892876	27.86246509172162	22.367450183421266
18-19	21.170051070289432	30.537579969875917	26.944762719979824	21.347606239854823
20-21	23.331636721420626	27.308108660439284	25.440114223275412	23.920140394864678
22-23	22.253433302796775	27.362767463867744	25.827282104248244	24.55651712908724
24-25	24.30597046290062	27.47667751446212	25.255846176117764	22.96150584651949
26-27	23.626460633873315	25.572397626700432	28.34671785623494	22.454423883191314
28-29	21.798617821355162	29.693394784861276	26.39069793634671	22.11728945743685
30-31	22.87902607138123	26.907470325354847	26.118921101809633	24.094582501454287
32-33	22.93906765725868	27.74289497205461	26.55747022942433	22.760567141262385
34-35	24.2285803769013	27.009295470123863	26.492735467156788	22.269388685818058
36-37	23.558209936051092	25.92360801849043	28.234884285085155	22.283297760373326
38-39	23.10828605393519	27.596557255760963	27.237205440718355	22.057951249585493
40-41	22.62901057710772	28.018236657874617	26.816158983261406	22.536593781756252
42-43	22.427215874553116	27.82577279580195	25.086917848912883	24.660093480732048
44-45	23.14588430416411	26.418828063411322	26.030841911405638	24.404445721018934
46-47	24.2667779106668	27.834410906563967	26.46558223912799	21.433228943641243
48-49	23.647196115643272	27.024509389782615	26.869978590430186	22.458315904143923
50-51	20.794513073608485	27.593196096984162	28.78292853665479	22.82936229275256
52-53	22.409003682068125	28.762181919689624	25.318597184659637	23.510217213582617
54-55	21.645046382556224	26.862115536878843	26.55503680549107	24.937801275073863
56-57	23.82275033787209	25.60626712542356	26.412599311315166	24.158383225389187
58-59	25.084973395541144	25.961352337113546	26.27349501850724	22.680179248838066
60-61	23.15441690627666	26.90297958976205	28.345131807247785	21.59747169671351
62-63	21.84814737678532	27.162414941220625	27.56658667491679	23.422851007077263
64-65	20.844272985014733	29.387182133024975	26.379385265647553	23.389159616312742
66-67	22.97276243849924	26.441548107290625	25.43451010692463	25.151179347285503
68-69	22.084547784533274	26.600094408468845	26.40002593760029	24.91533186939759
70-71	23.770168386674694	26.597747279031474	25.71510688101212	23.916977453281717
72-73	23.22578695238239	25.011629296760695	28.555260797111703	23.20732295374521
74-75	21.81211433030602	28.879729788211826	26.312209470464303	22.995946411017847
76-77	22.230933750680727	26.133014644343415	26.499191920635123	25.136859684340735
78-79	22.718104378389008	26.639187557764316	26.974146729978255	23.668561333868425
80-81	23.55442228273264	26.064293388214022	26.85852926710648	23.522755061946853
82-83	22.681954404697773	25.135958402492374	28.65979630729519	23.522290885514664
84-85	22.174057657435363	27.00635782330178	27.28461992575922	23.534964593503638
86-87	21.80653666496635	27.38792364315425	26.996058405010665	23.809481286868735
88-89	21.821236892433223	26.89950415552918	25.713120734618595	25.566138217419
90-91	22.721982975053315	25.25320840550932	26.387815608092392	25.636993011344973
92-93	23.769942990590014	26.49201181399821	26.721381963046912	23.01666323236486
94-95	22.424557630836304	26.070427668285323	27.394475195195493	24.11053950568288
96-97	20.80884843766012	26.27615198406299	28.874955389387797	24.04004418888909
98-99	22.301466486779088	27.305546930599483	26.055158807576163	24.33782777504527
100	22.163791464290814	24.312096377638497	25.96220654182951	27.561905616241177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2541.0
1	1662.0
2	805.5
3	806.5
4	758.5
5	640.5
6	517.5
7	506.0
8	572.0
9	600.0
10	509.5
11	469.5
12	470.5
13	455.5
14	504.0
15	607.5
16	709.0
17	865.0
18	1090.5
19	1323.5
20	1726.0
21	2282.5
22	3161.5
23	4549.0
24	6172.0
25	8124.5
26	11330.0
27	15762.0
28	19926.5
29	25123.5
30	31436.0
31	38204.0
32	45738.0
33	52279.5
34	58892.5
35	66494.0
36	74554.5
37	82878.5
38	87552.0
39	88878.5
40	89649.5
41	89760.0
42	90804.0
43	96859.0
44	109579.0
45	123320.5
46	138087.5
47	193428.5
48	303784.5
49	309344.5
50	225294.5
51	174245.0
52	135987.5
53	118660.5
54	108805.5
55	97734.0
56	88502.0
57	77125.0
58	65687.5
59	58762.0
60	49230.0
61	38287.5
62	30300.0
63	23630.5
64	17395.0
65	12205.0
66	8931.5
67	6644.0
68	4706.0
69	3281.0
70	2547.0
71	2050.5
72	1685.5
73	1612.5
74	1379.5
75	1012.0
76	691.0
77	493.5
78	369.0
79	256.0
80	181.0
81	119.0
82	86.0
83	65.0
84	47.0
85	37.5
86	32.5
87	31.5
88	28.0
89	22.0
90	19.0
91	14.5
92	15.0
93	15.5
94	16.0
95	14.0
96	11.0
97	12.0
98	11.5
99	10.5
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09557673505261662
2	0.06197939912372439
3	0.07811929579544712
4	0.07916235034225913
5	0.08067203455475021
6	0.08835769963652294
7	0.08424037905700184
8	0.08525598479995038
9	0.0730687158845679
10-11	0.07891531110748787
12-13	0.06886904889345637
14-15	0.07724093407181595
16-17	0.07938194077316693
18-19	0.07875061828430702
20-21	0.07018659147890313
22-23	0.07413921923524339
24-25	0.07617043072114046
26-27	0.07028266229242527
28-29	0.07901138192101004
30-31	0.08354043455848324
32-33	0.08510501637870126
34-35	0.0878636211669804
36-37	0.0880832115978882
38-39	0.08945565179106191
40-41	0.08550302403472164
42-43	0.09218680777547757
44-45	0.08774010154959477
46-47	0.0850775675748378
48-49	0.093833736007286
50-51	0.0904163599262835
52-53	0.09550811304295792
54-55	0.08783617236311693
56-57	0.09483561734830281
58-59	0.09948818960316168
60-61	0.09747070251919632
62-63	0.09892548912396044
64-65	0.10077828338474494
66-67	0.1032075025266624
68-69	0.09996854367077246
70-71	0.1022467943914408
72-73	0.09966660682827425
74-75	0.10371530539813667
76-77	0.10024303170940721
78-79	0.1069542642540266
80-81	0.10254873123393901
82-83	0.10676212262698229
84-85	0.10352316377109236
86-87	0.09962543362247904
88-89	0.09874707189884786
90-91	0.09893921352589219
92-93	0.09321613792035784
94-95	0.09325731112615306
96-97	0.0910476824151434
98-99	0.10529361162028641
100	0.24772545486785322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3643146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.9642563811949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34112166124126	40.48517079552917
2	14.44331496240009	16.16618763100214
3	5.6577660161889725	9.498980036244339
4	2.7301136953308807	6.111551311812353
5	1.5422415201099375	4.315519991657815
6	0.9317564251513221	3.1287033277196534
7	0.5962446094156932	2.3357870331071693
8	0.4182356128137295	1.8724996050602978
9	0.2839647077709859	1.430268633801589
>10	1.0360968391268408	8.824071871136912
>50	0.015607460042225694	0.5789599382820877
>100	0.0029355956776945472	0.2885043376074543
>500	0.0	0.0
>1k	3.755592064841223E-4	0.44671427057427704
>5k	0.0	0.0
>10k+	2.2533552389047336E-4	4.517081216464728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	76195	2.0914616103774044	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	19145	0.5255073499662105	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	12118	0.3326246052175784	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.234641159042212E-5	0.0
12-13	0.0	0.0	0.0	9.607081352215914E-5	1.3724401931737021E-5
14-15	0.0	0.0	0.0	1.2351961738563317E-4	2.7448803863474042E-5
16-17	0.0	0.0	0.0	1.6469282318084426E-4	4.1173205795211066E-5
18-19	0.0	0.0	0.0	2.4703923477126634E-4	5.4897607726948084E-5
20-21	0.0	0.0	0.0	4.2545645988384764E-4	5.4897607726948084E-5
22-23	0.0	0.0	0.0	8.783617236311693E-4	6.86220096586851E-5
24-25	0.0	0.0	0.0	0.0016332038298767055	8.234641159042212E-5
26-27	0.0	0.0	0.0	0.0029919196211186704	8.234641159042212E-5
28-29	0.0	0.0	0.0	0.008948310059492537	9.607081352215914E-5
30-31	0.0	0.0	0.0	0.024031427782471523	1.0979521545389617E-4
32-33	0.0	0.0	0.0	0.058863959885220085	1.0979521545389617E-4
34-35	0.0	0.0	0.0	0.1098089398558279	1.372440193173702E-4
36-37	0.0	0.0	0.0	0.18559508732287971	1.372440193173702E-4
38-39	0.0	0.0	0.0	0.2958157592366597	1.5096842124910723E-4
40-41	0.0	0.0	0.0	0.4212156196869409	1.6469282318084424E-4
42-43	0.0	0.0	0.0	0.5440078437701921	1.7841722511258127E-4
44-45	0.0	0.0	0.0	0.6929450535334022	1.921416270443183E-4
46-47	0.0	0.0	0.0	0.8811203284194484	1.921416270443183E-4
48-49	0.0	1.3724401931737021E-5	0.0	1.0675251554563008	2.1959043090779233E-4
50-51	0.0	2.7448803863474042E-5	0.0	1.2674485183959139	2.1959043090779233E-4
52-53	0.0	2.7448803863474042E-5	0.0	1.4738360746453751	2.1959043090779233E-4
54-55	0.0	2.7448803863474042E-5	0.0	1.7023885400145917	2.3331483283952937E-4
56-57	0.0	2.7448803863474042E-5	0.0	1.9999610226985138	2.470392347712664E-4
58-59	0.0	2.7448803863474042E-5	0.0	2.304025147496148	2.470392347712664E-4
60-61	0.0	2.7448803863474042E-5	0.0	2.613016332587275	2.470392347712664E-4
62-63	0.0	2.7448803863474042E-5	0.0	2.929157931084837	2.744880386347404E-4
64-65	0.0	2.7448803863474042E-5	0.0	3.2675742339176086	3.0193684249821447E-4
66-67	0.0	2.7448803863474042E-5	0.0	3.6597764679208575	3.0193684249821447E-4
68-69	0.0	2.7448803863474042E-5	0.0	4.1007963995952945	3.0193684249821447E-4
70-71	0.0	2.7448803863474042E-5	0.0	4.57837813801588	3.0193684249821447E-4
72-73	0.0	2.7448803863474042E-5	0.0	5.029005151042533	3.156612444299515E-4
74-75	0.0	2.7448803863474042E-5	0.0	5.498997295194867	3.293856463616885E-4
76-77	0.0	2.7448803863474042E-5	0.0	5.980710078596905	3.293856463616885E-4
78-79	0.0	2.7448803863474042E-5	0.0	6.609013199031826	3.431100482934255E-4
80-81	0.0	2.7448803863474042E-5	0.0	7.255061971164483	3.5683445022516254E-4
82-83	0.0	2.7448803863474042E-5	0.0	7.869201508805851	3.5683445022516254E-4
84-85	0.0	2.7448803863474042E-5	0.0	8.49069732588263	3.5683445022516254E-4
86-87	0.0	2.7448803863474042E-5	0.0	9.11282446544827	3.5683445022516254E-4
88	0.0	2.7448803863474042E-5	0.0	9.632855779043716	3.5683445022516254E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1205	0.0	23.805428	1
TGATCGC	885	0.0	22.30062	9
CTAACGC	510	0.0	22.123554	3
GTGATCG	910	0.0	21.689455	8
CATGGGG	6585	0.0	21.274939	4
CTAGTAC	1685	0.0	21.204473	3
TAGTACT	1900	0.0	20.78421	4
GGCGTCG	775	0.0	20.616642	8
TGCGCGA	930	0.0	19.733927	94
GTATAAG	2090	0.0	19.350197	1
CGGGCGT	805	0.0	19.26534	6
TAGGCAT	2005	0.0	19.22602	5
ATGGGGG	4650	0.0	19.208374	5
GCATCGT	1420	0.0	19.194658	8
ATACGGG	835	0.0	19.142834	3
TACGGGC	825	0.0	18.804762	4
GTACCCG	485	0.0	18.422352	1
CGTTAAC	360	1.10958354E-10	18.287685	1
CTGTGCG	1840	0.0	18.132238	9
CTAGGCA	2080	0.0	17.855482	4
>>END_MODULE