FastQCFastQC Report
Mon 16 Jan 2017
ERR1146612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643146
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT799892.195602372235425No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT183630.5040423853449738No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG136770.37541729044073446No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA131730.36158309329354354No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA126330.3467607392072676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101350.2781936271563094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95070.26095577833004774No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG76230.20924223185126262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55460.15223106622682703No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA38760.10639156377482539No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG1005.3277152E-528.199187
GTACTAG11250.026.3318841
CTAACGC5300.025.7268283
CTAGTAC19000.023.756463
TAGCGCG1000.001755495223.5089954
GTGATCG7900.023.2018598
TAACGCC6650.022.62524
TAGTACT22500.022.3596694
GAGTACT109750.021.94727112-13
TGATCGC8150.021.9134739
CTGTGCG21300.021.4031359
AGTACTT116700.020.62007712-13
TAGGCAT19450.020.2984495
TCTAACG6750.020.2014332
GTACTTT126050.019.05325714-15
CCTAGTA23600.018.9277742
GAGTACG11950.018.8871861
GTATAAG19900.018.6667181
CATGGGG64800.018.6475684
CTAGGCA22150.018.2552914
Produced by FastQC (version 0.11.5)