##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146612_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3643146 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34199974417715 33.0 31.0 34.0 30.0 34.0 2 32.36474876384312 34.0 31.0 34.0 30.0 34.0 3 32.50010787379918 34.0 31.0 34.0 30.0 34.0 4 35.96454657595386 37.0 35.0 37.0 35.0 37.0 5 36.028276110811916 37.0 35.0 37.0 35.0 37.0 6 36.126954835189146 37.0 36.0 37.0 35.0 37.0 7 36.10555849257757 37.0 36.0 37.0 35.0 37.0 8 36.1117767446048 37.0 36.0 37.0 35.0 37.0 9 37.885948573019036 39.0 38.0 39.0 35.0 39.0 10-11 37.83813563881327 39.0 38.0 39.0 35.0 39.0 12-13 37.82716311671287 39.0 38.0 39.0 35.0 39.0 14-15 39.104468500576154 40.0 38.5 41.0 36.0 41.0 16-17 39.08324906550547 40.0 38.5 41.0 36.0 41.0 18-19 39.21155987160547 40.0 39.0 41.0 36.0 41.0 20-21 39.26856842959354 40.0 39.0 41.0 36.0 41.0 22-23 39.229119420413014 40.0 39.0 41.0 36.0 41.0 24-25 39.20178617601381 40.5 39.0 41.0 36.0 41.0 26-27 39.0885991118665 40.0 39.0 41.0 35.5 41.0 28-29 39.05590086150816 40.0 39.0 41.0 35.0 41.0 30-31 38.971758337436924 40.0 38.5 41.0 35.0 41.0 32-33 38.85460341144714 40.0 38.0 41.0 35.0 41.0 34-35 38.76117619222507 40.0 38.0 41.0 35.0 41.0 36-37 38.68265696735733 40.0 38.0 41.0 35.0 41.0 38-39 38.603480206393044 40.0 38.0 41.0 35.0 41.0 40-41 38.576906607640765 40.0 38.0 41.0 35.0 41.0 42-43 38.48563576645021 40.0 38.0 41.0 34.5 41.0 44-45 38.34855932208042 40.0 38.0 41.0 34.0 41.0 46-47 38.1201580996205 40.0 38.0 41.0 33.5 41.0 48-49 37.824407119560945 40.0 37.0 41.0 33.0 41.0 50-51 37.678324310911506 40.0 37.0 41.0 33.0 41.0 52-53 37.66033409037135 40.0 37.0 41.0 33.0 41.0 54-55 37.511516008416905 40.0 36.5 41.0 33.0 41.0 56-57 37.30830455326249 39.5 36.0 41.0 32.0 41.0 58-59 37.1264379742124 39.0 36.0 41.0 32.0 41.0 60-61 36.816723924871525 39.0 35.0 41.0 31.0 41.0 62-63 36.62244225183399 39.0 35.0 41.0 31.0 41.0 64-65 36.35722490946012 38.0 35.0 40.0 31.0 41.0 66-67 36.02667337515433 37.0 35.0 40.0 31.0 41.0 68-69 35.662452314565485 37.0 35.0 39.0 31.0 41.0 70-71 35.248744079979225 36.0 35.0 39.0 30.0 41.0 72-73 34.880343115538054 36.0 34.5 38.5 29.5 40.5 74-75 34.60208910101325 35.0 34.0 37.0 30.0 40.0 76-77 34.31045640224136 35.0 34.0 37.0 30.0 39.0 78-79 34.01640038582039 35.0 34.0 36.5 29.0 39.0 80-81 33.75234275540975 35.0 34.0 36.0 29.0 38.5 82-83 33.487716111295015 35.0 34.0 36.0 29.0 37.0 84-85 33.308056553319574 35.0 34.0 35.0 29.0 37.0 86-87 33.21845706979627 35.0 34.0 35.0 29.0 36.5 88-89 33.034740853097844 35.0 34.0 35.0 29.0 36.0 90-91 32.93217675053374 35.0 34.0 35.0 29.0 36.0 92-93 32.82138363381539 35.0 34.0 35.0 29.0 36.0 94-95 32.73462427802784 35.0 33.5 35.0 28.5 35.5 96-97 32.66582838019667 35.0 33.0 35.0 28.5 35.0 98-99 32.6611550840949 35.0 34.0 35.0 29.0 35.0 100 31.830782241502263 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31510552624068566 1101 2 0.31581068976245774 1101 3 0.28448286764454167 1101 4 0.20457117592864904 1101 5 0.16584234098871775 1101 6 0.22837412147427472 1101 7 0.1992227067047665 1101 8 0.23388813650110052 1101 9 0.23415342347409052 1101 10-11 0.2534579197823703 1101 12-13 0.2909891377845213 1101 14-15 0.4541439072531901 1101 16-17 0.4179378153350939 1101 18-19 0.37893964293855475 1101 20-21 0.33819344431167053 1101 22-23 0.32565714920770006 1101 24-25 0.3957816466835382 1101 26-27 0.33146274943864285 1101 28-29 0.3516538370979845 1101 30-31 0.43092683828068346 1101 32-33 0.4147147530106281 1101 34-35 0.41104939655685513 1101 36-37 0.38976206969852 1101 38-39 0.40412494297735435 1101 40-41 0.45024462051267733 1101 42-43 0.4503015388891214 1101 44-45 0.4222981543672688 1101 46-47 0.41669140082930767 1101 48-49 0.45823984638756343 1101 50-51 0.44993733804219005 1101 52-53 0.4342398823956799 1101 54-55 0.4362124939631755 1101 56-57 0.3947705344257386 1101 58-59 0.36141881701921363 1101 60-61 0.3799842443879342 1101 62-63 0.3961324928926686 1101 64-65 0.32289888875840234 1101 66-67 0.309907685127385 1101 68-69 0.24968512588913683 1101 70-71 0.2987109518651323 1101 72-73 0.3465868487541073 1101 74-75 0.3049326602270739 1101 76-77 0.20343787738252672 1101 78-79 0.2207218523722716 1101 80-81 0.20268634633821137 1101 82-83 0.2619025007386071 1101 84-85 0.2711596212581142 1101 86-87 0.2981805338206627 1101 88-89 0.37635932963068797 1101 90-91 0.3234604220280275 1101 92-93 0.34077477633233144 1101 94-95 0.38106071126896524 1101 96-97 0.29363741553134304 1101 98-99 0.3431163897786291 1101 100 0.3414677224349987 1102 1 0.27656477417841785 1102 2 0.23521656274219538 1102 3 0.28272059433093943 1102 4 0.17646314692146348 1102 5 0.14427962758102808 1102 6 0.16391795202721227 1102 7 0.20510164831100752 1102 8 0.21118833270067938 1102 9 0.30145042194568106 1102 10-11 0.22869124552786957 1102 12-13 0.2967293305153902 1102 14-15 0.42503563977137304 1102 16-17 0.5165091355089686 1102 18-19 0.3980562299330259 1102 20-21 0.3778164105024757 1102 22-23 0.3677028008396235 1102 24-25 0.3740407547884175 1102 26-27 0.3628466881723469 1102 28-29 0.3818390343273421 1102 30-31 0.4404969877609233 1102 32-33 0.39699635902886143 1102 34-35 0.3994584455850756 1102 36-37 0.4883474815550528 1102 38-39 0.4706085532664517 1102 40-41 0.5091872836239091 1102 42-43 0.5320029469734067 1102 44-45 0.4499471197125686 1102 46-47 0.5092344617595757 1102 48-49 0.6491428522518632 1102 50-51 0.6588253958831274 1102 52-53 0.5186888976038517 1102 54-55 0.530009985643666 1102 56-57 0.5611695946189101 1102 58-59 0.5331496821401274 1102 60-61 0.5537327945705854 1102 62-63 0.5197371453158937 1102 64-65 0.5254653364620054 1102 66-67 0.5002058326131049 1102 68-69 0.5240686147729647 1102 70-71 0.47278862413595846 1102 72-73 0.501236562042557 1102 74-75 0.4839154575344722 1102 76-77 0.4621286516662835 1102 78-79 0.42008894612531833 1102 80-81 0.40674136351921675 1102 82-83 0.42758125915296574 1102 84-85 0.38991971578077766 1102 86-87 0.4385816589873315 1102 88-89 0.4536115599104633 1102 90-91 0.4334334039364691 1102 92-93 0.460566002633918 1102 94-95 0.4559378155365792 1102 96-97 0.42580073271344787 1102 98-99 0.44411039539362207 1102 100 0.5081996986308965 1103 1 0.2620959557387579 1103 2 0.2265777566291476 1103 3 0.2454696698344705 1103 4 0.23048365937373916 1103 5 0.19221940665928372 1103 6 0.1892258183252764 1103 7 0.16801049116455857 1103 8 0.1841774780949521 1103 9 0.202154714720848 1103 10-11 0.21716376195339393 1103 12-13 0.21032152285852135 1103 14-15 0.2993382283614707 1103 16-17 0.38879847597498696 1103 18-19 0.31807531824187407 1103 20-21 0.28750507360280153 1103 22-23 0.35350983539007075 1103 24-25 0.34205646823640024 1103 26-27 0.37849012574340435 1103 28-29 0.36046162435354745 1103 30-31 0.4021528237570422 1103 32-33 0.41152770813548045 1103 34-35 0.3829793709192657 1103 36-37 0.4031065140949295 1103 38-39 0.3800797053865068 1103 40-41 0.3759688391714846 1103 42-43 0.357602523320665 1103 44-45 0.3407880225476916 1103 46-47 0.4409159682400201 1103 48-49 0.5463187997859436 1103 50-51 0.5247970446582428 1103 52-53 0.4678906592333618 1103 54-55 0.46795144018476975 1103 56-57 0.4079365497045373 1103 58-59 0.4107646009400199 1103 60-61 0.4547162314235891 1103 62-63 0.45118737808555665 1103 64-65 0.4542548743932855 1103 66-67 0.3990485664897676 1103 68-69 0.4033802388948402 1103 70-71 0.39246514013063916 1103 72-73 0.4534730057529899 1103 74-75 0.40262588968421653 1103 76-77 0.32765480683807624 1103 78-79 0.28606897221467165 1103 80-81 0.34807473275532885 1103 82-83 0.34392450165227473 1103 84-85 0.34547746539482915 1103 86-87 0.3538941926461803 1103 88-89 0.3610452295331257 1103 90-91 0.28208585310412815 1103 92-93 0.33298190038281206 1103 94-95 0.31323954246007446 1103 96-97 0.3496590244593918 1103 98-99 0.34056379957510075 1103 100 0.3768110989588038 1104 1 0.2184506269558284 1104 2 0.16869651984755762 1104 3 0.19355781606920175 1104 4 0.14808869527178103 1104 5 0.10760154725994653 1104 6 0.14795974103437715 1104 7 0.16078960335295278 1104 8 0.12351635266779937 1104 9 0.1439341465016355 1104 10-11 0.17162268597706287 1104 12-13 0.15914980149393898 1104 14-15 0.29029319859646563 1104 16-17 0.3308172130002447 1104 18-19 0.24896860354188988 1104 20-21 0.2506198159525326 1104 22-23 0.2667534016479891 1104 24-25 0.2933860159861439 1104 26-27 0.2681575285154878 1104 28-29 0.27089862148901034 1104 30-31 0.30599339547850946 1104 32-33 0.31130983736776585 1104 34-35 0.25373907937762397 1104 36-37 0.2534106920078045 1104 38-39 0.2510222048021191 1104 40-41 0.26800865438777777 1104 42-43 0.2711436700627132 1104 44-45 0.2619208399683757 1104 46-47 0.3548611921357008 1104 48-49 0.37584363666851317 1104 50-51 0.3252856598141278 1104 52-53 0.29825266518587057 1104 54-55 0.29825967565965783 1104 56-57 0.24698103398382187 1104 58-59 0.18069929909066218 1104 60-61 0.2039121109259554 1104 62-63 0.17853329843382681 1104 64-65 0.1507228829657663 1104 66-67 0.18654608272780138 1104 68-69 0.1861515635782709 1104 70-71 0.18096942972885444 1104 72-73 0.15370253156863356 1104 74-75 0.1555825492868692 1104 76-77 0.08756344043564468 1104 78-79 0.1484425747351068 1104 80-81 0.11921728046198865 1104 82-83 0.15294423513089583 1104 84-85 0.15367530504200744 1104 86-87 0.19317677407466505 1104 88-89 0.2611105649634382 1104 90-91 0.22011957526121506 1104 92-93 0.11339608786052224 1104 94-95 0.16052276553048017 1104 96-97 0.22393854920208867 1104 98-99 0.16602188063493628 1104 100 0.27866494777014594 1105 1 0.1380855925139599 1105 2 0.14722930877579898 1105 3 0.08469779210093975 1105 4 0.12015960827876881 1105 5 0.0660600542718015 1105 6 0.08158716901671426 1105 7 0.13070644314115754 1105 8 0.11398866530296203 1105 9 0.14151616112076226 1105 10-11 0.15161820137994653 1105 12-13 0.1722501376897725 1105 14-15 0.24582216603077 1105 16-17 0.3009929044061863 1105 18-19 0.2238906654299413 1105 20-21 0.19333989635293136 1105 22-23 0.2501597048481585 1105 24-25 0.2289254322032761 1105 26-27 0.25160238733469953 1105 28-29 0.26519357384619013 1105 30-31 0.29798305027452443 1105 32-33 0.23751161353098382 1105 34-35 0.18316260813606533 1105 36-37 0.1803789783211016 1105 38-39 0.2480314994752888 1105 40-41 0.2608115100166728 1105 42-43 0.24557881210206745 1105 44-45 0.30450365930954604 1105 46-47 0.3166460235951405 1105 48-49 0.3772767637327874 1105 50-51 0.3620278089238127 1105 52-53 0.30272175603024465 1105 54-55 0.23969395767382906 1105 56-57 0.26981753287542887 1105 58-59 0.2931291456236309 1105 60-61 0.33202658952909303 1105 62-63 0.3447406331753555 1105 64-65 0.32609482533712963 1105 66-67 0.2440158586743877 1105 68-69 0.28764643310292115 1105 70-71 0.2750102510968304 1105 72-73 0.2932016499645229 1105 74-75 0.27320438065088837 1105 76-77 0.23269586132290954 1105 78-79 0.2403601062710834 1105 80-81 0.27770591012539114 1105 82-83 0.2527513271801709 1105 84-85 0.1543142512816118 1105 86-87 0.21661818943216105 1105 88-89 0.2700971369140248 1105 90-91 0.25460861725395745 1105 92-93 0.2791158343795743 1105 94-95 0.30971307461478403 1105 96-97 0.21948742806426935 1105 98-99 0.25378069501470435 1105 100 0.2797426718497107 1106 1 0.06633582157444806 1106 2 0.06739470845030127 1106 3 0.12188153728066453 1106 4 0.06712156185530915 1106 5 0.08587682632661853 1106 6 0.07980952670802566 1106 7 0.10766975278792756 1106 8 0.055457293016111464 1106 9 0.09368222590499187 1106 10-11 0.08837758858808797 1106 12-13 0.11416419832706737 1106 14-15 0.1904840214839041 1106 16-17 0.2644530735126054 1106 18-19 0.18574962010499263 1106 20-21 0.2220989365473045 1106 22-23 0.16281034311032272 1106 24-25 0.17788886730662767 1106 26-27 0.21677407480819966 1106 28-29 0.18909685660960918 1106 30-31 0.18866237500850502 1106 32-33 0.21227101695063766 1106 34-35 0.1752256356807962 1106 36-37 0.1998981013577179 1106 38-39 0.20308490943693158 1106 40-41 0.18939245006617256 1106 42-43 0.17628955647947464 1106 44-45 0.11155702823126035 1106 46-47 0.16966619355155643 1106 48-49 0.22935861998773532 1106 50-51 0.13434263103434319 1106 52-53 0.11568527628049452 1106 54-55 0.19553738625752004 1106 56-57 0.14057593395689594 1106 58-59 0.1453903872596527 1106 60-61 0.15114480285215848 1106 62-63 0.11060576034326886 1106 64-65 0.15071308280367646 1106 66-67 0.10795734143239599 1106 68-69 0.08417643778161477 1106 70-71 0.09065292286626914 1106 72-73 0.08133553886067091 1106 74-75 0.11384104430626252 1106 76-77 0.12352569013602022 1106 78-79 0.06843375045095712 1106 80-81 0.13700126404767587 1106 82-83 0.12078427902959987 1106 84-85 0.10237305536495 1106 86-87 0.08199884462657536 1106 88-89 0.14612398403759386 1106 90-91 0.1580548101470285 1106 92-93 0.1510755479208754 1106 94-95 0.20423666129129714 1106 96-97 0.14125300156695886 1106 98-99 0.0832516866861468 1106 100 0.13168937748630327 1107 1 0.10210436755254193 1107 2 0.09343706040971966 1107 3 0.1329917246358363 1107 4 0.02199340110515635 1107 5 0.03908215163639994 1107 6 0.09583710927351063 1107 7 0.08291303068126865 1107 8 0.05979410289783971 1107 9 0.1677165393937372 1107 10-11 0.12995841440796596 1107 12-13 0.14094602911156784 1107 14-15 0.203788904235914 1107 16-17 0.2056880444008371 1107 18-19 0.15143182928793664 1107 20-21 0.12954705990091497 1107 22-23 0.13856216871250382 1107 24-25 0.16506055882003778 1107 26-27 0.1890976603604244 1107 28-29 0.20256821017471793 1107 30-31 0.1935005270666963 1107 32-33 0.1308604336063155 1107 34-35 0.1627844962053402 1107 36-37 0.11031734720368291 1107 38-39 0.13917204971090769 1107 40-41 0.16051108294264083 1107 42-43 0.13377781836626923 1107 44-45 0.12207361780994574 1107 46-47 0.18511604113418656 1107 48-49 0.25463298966475634 1107 50-51 0.18456777647987366 1107 52-53 0.17454792530754304 1107 54-55 0.16775005618239192 1107 56-57 0.1284877239357698 1107 58-59 0.11839008898247982 1107 60-61 0.1072687759664177 1107 62-63 0.10244854886942534 1107 64-65 0.09859734375399398 1107 66-67 0.10349387705775825 1107 68-69 0.06682142619398945 1107 70-71 0.06441164864205007 1107 72-73 0.08330918197925286 1107 74-75 0.11229929052996113 1107 76-77 0.09422581790232698 1107 78-79 0.07300972381393223 1107 80-81 0.09517769853638924 1107 82-83 0.03742158064377321 1107 84-85 0.10572088379807099 1107 86-87 0.06929058703691027 1107 88-89 0.10536625308558456 1107 90-91 0.10708266167184632 1107 92-93 0.12697286508340966 1107 94-95 0.14257484882832472 1107 96-97 0.18480520439204184 1107 98-99 0.1273371166110593 1107 100 0.1133848820980532 1108 1 0.042152554906088824 1108 2 0.05255828799048601 1108 3 0.015023204279110303 1108 4 0.04552742901982754 1108 5 0.05645990762465658 1108 6 0.0864976159480193 1108 7 0.029285438405771913 1108 8 0.03233937300439038 1108 9 0.10041634358386631 1108 10-11 0.08586586311645306 1108 12-13 0.05240065761741164 1108 14-15 0.11653390524843843 1108 16-17 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2214 84-85 -0.2629908283629945 2214 86-87 -0.2248651744207919 2214 88-89 -0.22540644715012803 2214 90-91 -0.22213031968623653 2214 92-93 -0.2148232661249736 2214 94-95 -0.18893746701426295 2214 96-97 -0.19207167235746425 2214 98-99 -0.22125086990739362 2214 100 -0.4472364244187652 2215 1 -0.3399678032529039 2215 2 -0.37259396152587243 2215 3 -0.3735968845135318 2215 4 -0.2813828102533549 2215 5 -0.21169741745350734 2215 6 -0.2954300824886076 2215 7 -0.2584358063557204 2215 8 -0.2749233573794925 2215 9 -0.39143520618943484 2215 10-11 -0.3533922093889572 2215 12-13 -0.3461410128364264 2215 14-15 -0.3626753944526442 2215 16-17 -0.3645405122462506 2215 18-19 -0.4381951074855195 2215 20-21 -0.4063707864072228 2215 22-23 -0.39610005324627195 2215 24-25 -0.38879985271224626 2215 26-27 -0.4563072295955237 2215 28-29 -0.46325246594361147 2215 30-31 -0.4027332427613999 2215 32-33 -0.41219674336529266 2215 34-35 -0.45228679876303346 2215 36-37 -0.4583704586119737 2215 38-39 -0.4406111724411659 2215 40-41 -0.48128473827501494 2215 42-43 -0.5215465965673758 2215 44-45 -0.5157843961210773 2215 46-47 -0.4905466879340423 2215 48-49 -0.4389843449357329 2215 50-51 -0.39772413376240934 2215 52-53 -0.41796887609798006 2215 54-55 -0.4878197140798122 2215 56-57 -0.4952729415434973 2215 58-59 -0.49685017890728744 2215 60-61 -0.5773349170194564 2215 62-63 -0.5218284333521055 2215 64-65 -0.36923223325744914 2215 66-67 -0.36642818019832646 2215 68-69 -0.3866064168387524 2215 70-71 -0.3789871897787549 2215 72-73 -0.3614206280584611 2215 74-75 -0.41678625297172545 2215 76-77 -0.3562777012454674 2215 78-79 -0.4096573264718444 2215 80-81 -0.3521029679104686 2215 82-83 -0.3877327041913432 2215 84-85 -0.39341014373817274 2215 86-87 -0.35732062956159183 2215 88-89 -0.2919489053027604 2215 90-91 -0.3606402687190524 2215 92-93 -0.33161166212909876 2215 94-95 -0.33878476564645865 2215 96-97 -0.36098487735784346 2215 98-99 -0.38550248273107 2215 100 -0.581439997340869 2216 1 -0.45311936405837017 2216 2 -0.4480320858516329 2216 3 -0.410527952844042 2216 4 -0.31960339543466887 2216 5 -0.25511084513223636 2216 6 -0.29626529734579776 2216 7 -0.32479981603230357 2216 8 -0.3107744987397396 2216 9 -0.39878110279978785 2216 10-11 -0.4242037555370217 2216 12-13 -0.3948787667655722 2216 14-15 -0.5113851731151939 2216 16-17 -0.49134307404555244 2216 18-19 -0.5291280785425485 2216 20-21 -0.46565743140291715 2216 22-23 -0.5178108204363099 2216 24-25 -0.5196292601491237 2216 26-27 -0.5345259116419143 2216 28-29 -0.5607710087466913 2216 30-31 -0.5574994828631077 2216 32-33 -0.6217078847704585 2216 34-35 -0.5900550626812873 2216 36-37 -0.5461849123499718 2216 38-39 -0.5895916475428749 2216 40-41 -0.5912074530275646 2216 42-43 -0.5850812755859067 2216 44-45 -0.5601581820302357 2216 46-47 -0.5681075530272963 2216 48-49 -0.46683764345448964 2216 50-51 -0.5530527073525775 2216 52-53 -0.5299598411078676 2216 54-55 -0.5021537710264354 2216 56-57 -0.5600481704863469 2216 58-59 -0.655025531962302 2216 60-61 -0.6796459599190143 2216 62-63 -0.6286882546003696 2216 64-65 -0.5639393638670214 2216 66-67 -0.6287396866296717 2216 68-69 -0.5714532359182058 2216 70-71 -0.5583000927272508 2216 72-73 -0.5454527644928149 2216 74-75 -0.5065857254439621 2216 76-77 -0.51570669338237 2216 78-79 -0.4776289848721973 2216 80-81 -0.5119918570943014 2216 82-83 -0.506810090108921 2216 84-85 -0.5221284089122449 2216 86-87 -0.5305965006082687 2216 88-89 -0.3764381208374843 2216 90-91 -0.4936559330305528 2216 92-93 -0.5390117327411232 2216 94-95 -0.6046885617676239 2216 96-97 -0.6354554556474383 2216 98-99 -0.658534101112501 2216 100 -0.7716599666579249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 30.0 8 102.0 9 282.0 10 326.0 11 217.0 12 224.0 13 252.0 14 419.0 15 783.0 16 1407.0 17 2594.0 18 4413.0 19 6586.0 20 9330.0 21 13669.0 22 19643.0 23 22841.0 24 22504.0 25 21096.0 26 21328.0 27 24780.0 28 30923.0 29 37697.0 30 46741.0 31 58705.0 32 75398.0 33 99602.0 34 144059.0 35 223665.0 36 421441.0 37 924440.0 38 1089893.0 39 316925.0 40 831.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01770348022505 19.112319859986183 13.281933446772488 22.588043213016277 2 14.500572054741575 22.8635786690559 37.17927602684375 25.456573249358772 3 21.789896512292426 25.595132547820036 30.626042713726463 21.98892822616107 4 11.955237853827118 17.174458179804912 40.17352636743397 30.696777598934005 5 11.804060506560038 40.00905287145938 35.21363870808003 12.973247913900549 6 28.88342265600724 38.001554774508286 17.508753841643582 15.606268727840893 7 25.0435475273294 33.61339347915236 23.228907103915134 18.114151889603107 8 25.128824629144546 31.432111554594112 20.822034672922822 22.61702914333852 9 24.535874655482917 17.495168320321216 22.630212429212907 35.338744594982956 10-11 25.025767599991326 24.86268030800894 28.082827428309802 22.028724663689932 12-13 28.145619198352197 23.320339069584364 27.10334694245029 21.430694789613153 14-15 23.76657753581349 24.84498629000681 28.09610541220492 23.29233076197478 16-17 21.127267981111324 27.746593559311748 28.610725568675665 22.515412890901256 18-19 20.75639136069553 29.378265812147514 28.721936472117942 21.14340635503901 20-21 22.82716477212293 26.14836799324758 27.55884179501872 23.465625439610776 22-23 21.71414486270932 26.253216313592702 27.955000979922296 24.077637843775683 24-25 23.747099347651726 26.443985500443844 27.3981882691498 22.41072688275463 26-27 23.15335238011323 24.53727016192508 30.393201131278264 21.916176326683427 28-29 21.239204039367085 28.641750465600463 28.54020358783414 21.578841907198314 30-31 22.491986646125763 25.74753217521985 28.206113549104312 23.55436762955008 32-33 22.564177168717897 26.60290752133292 28.62936136777709 22.203553942172096 34-35 23.731286747146694 25.962987971914337 28.591915215994995 21.713810064943978 36-37 23.137755977321792 24.833625891914814 30.192320097058996 21.836298033704395 38-39 22.65999088968073 26.48670926841172 29.290431296348473 21.562868545559077 40-41 22.247385281929507 26.881206721588352 28.68947472871519 22.181933267766947 42-43 22.176121956133517 26.793106836783377 26.875947326843335 24.15482388023977 44-45 22.953423946869826 25.367366363104228 27.646891304243397 24.032318385782546 46-47 24.132631522316153 26.925300276189862 27.814147442896882 21.127920758597103 48-49 23.69529623443481 26.327843113573575 27.89097057624662 22.085890075744995 50-51 20.70114675612781 27.019957476313056 29.645490463462075 22.633405304097064 52-53 22.33498831775701 28.1700985085377 26.17191617331881 23.32299700038648 54-55 21.633567444759834 26.34507019637197 27.295679520149807 24.72568283871839 56-57 23.78871433420067 25.06920191312804 27.22794202314567 23.914141729525614 58-59 24.971494260698094 25.43153873898405 27.054252309692277 22.54271469062558 60-61 22.969892198333547 26.496795128249083 29.148611542479113 21.384701130938254 62-63 21.68807561054776 26.713956426354446 28.349591046740553 23.248376916357238 64-65 20.71922376765512 29.000948633265406 27.09472004339119 23.185107555688287 66-67 22.82850918589278 26.054197872232088 26.26364783858642 24.853645103288713 68-69 21.988281572208898 26.245383180730624 27.15236659895355 24.613968648106933 70-71 23.588537701465665 26.249501958744958 26.449500778406055 23.71245956138332 72-73 23.142048243172468 24.620468487341903 29.23872179419189 22.99876147529374 74-75 21.764249735798302 28.610724530269966 26.797628360851483 22.82739737308025 76-77 22.3250435046844 25.81675507140615 26.846154342363853 25.012047081545603 78-79 22.663855330079517 26.487363670625104 27.291361467011537 23.557419532283845 80-81 23.481640686117082 25.92146545122162 27.13146185539278 23.465432007268518 82-83 22.577764346074602 24.99840795888801 28.898414601542306 23.525413093495086 84-85 22.087469315525198 26.86584801142095 27.49717344623262 23.54950922682123 86-87 21.7859646580181 27.27687828047517 27.0499452945339 23.88721176697283 88-89 21.66794852580709 26.881848819673987 25.8216799436531 25.62852271086583 90-91 22.595452911626207 25.165842310125225 26.484483390653324 25.75422138759525 92-93 23.56868133677944 26.654601080824698 26.65250124617638 23.124216336219487 94-95 22.247264691257786 26.16684570156358 27.412061201525223 24.173828405653413 96-97 20.554416196294973 26.38279808609603 28.919339814848744 24.14344590276025 98-99 22.10724973113867 27.30183177048822 26.045512362680345 24.54540613569276 100 21.871148277793452 24.417504917705855 26.15403674908671 27.557310055413982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 511.0 1 319.0 2 355.5 3 610.0 4 416.0 5 269.5 6 522.5 7 1284.0 8 2360.5 9 2834.0 10 2114.0 11 1373.5 12 1347.5 13 1465.0 14 1706.0 15 2048.0 16 2369.0 17 2783.0 18 3307.5 19 3771.5 20 4485.5 21 5253.0 22 6212.5 23 7619.5 24 9099.0 25 10813.0 26 13832.5 27 18094.5 28 21806.0 29 26365.5 30 32106.5 31 38621.5 32 45703.0 33 51789.5 34 58140.0 35 64803.0 36 72384.0 37 80718.0 38 85189.0 39 86696.5 40 87773.0 41 87455.5 42 88059.5 43 94026.0 44 106701.5 45 120489.5 46 133089.0 47 194230.5 48 312485.0 49 309958.0 50 221657.5 51 174075.5 52 135770.5 53 117205.5 54 106705.0 55 96345.0 56 86988.0 57 76149.0 58 65489.0 59 58472.0 60 48642.5 61 37401.0 62 29153.0 63 22748.0 64 16634.5 65 11453.0 66 8366.5 67 6232.5 68 4373.0 69 3034.0 70 2256.5 71 1837.5 72 1569.5 73 1491.0 74 1186.0 75 863.0 76 595.5 77 357.0 78 218.0 79 132.5 80 97.5 81 63.5 82 35.0 83 13.0 84 8.5 85 3.5 86 2.5 87 2.5 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021135578974875013 2 0.0037879349331594175 3 2.1959043090779236E-4 4 0.05091753116674434 5 0.0028272267979378264 6 0.004776091872244483 7 0.0 8 4.117320579521106E-4 9 8.234641159042212E-5 10-11 1.372440193173702E-4 12-13 0.0 14-15 1.3724401931737023E-5 16-17 2.8821244056647744E-4 18-19 1.5096842124910723E-4 20-21 2.3331483283952934E-4 22-23 0.0 24-25 0.0 26-27 2.8821244056647744E-4 28-29 2.7448803863474045E-5 30-31 2.3331483283952934E-4 32-33 2.3331483283952934E-4 34-35 1.6469282318084424E-4 36-37 2.7448803863474045E-5 38-39 6.72495694655114E-4 40-41 5.215272734060067E-4 42-43 0.0 44-45 5.078028714742698E-4 46-47 0.0 48-49 2.7448803863474045E-5 50-51 0.0 52-53 2.744880386347404E-4 54-55 2.470392347712664E-4 56-57 6.86220096586851E-5 58-59 5.489760772694808E-4 60-61 5.627004792012178E-4 62-63 9.607081352215914E-5 64-65 2.744880386347404E-4 66-67 8.920861255629063E-4 68-69 0.0022096287110096604 70-71 0.0034173760810025182 72-73 0.002799777994074352 74-75 0.002635085170893508 76-77 0.004048698569862421 78-79 0.0010705033506754876 80-81 9.881569390850656E-4 82-83 6.58771292723377E-4 84-85 4.666296656790587E-4 86-87 0.0 88-89 0.0 90-91 4.117320579521106E-5 92-93 5.489760772694809E-5 94-95 4.117320579521106E-5 96-97 4.5290526374732165E-4 98-99 1.0979521545389618E-4 100 0.07490778574342065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3643146.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.661706898562734 #Duplication Level Percentage of deduplicated Percentage of total 1 71.09937692201113 37.44214548137385 2 14.866554182612893 15.657962379127241 3 6.0425038673077 9.546257027807698 4 2.920053014174857 6.150999038429639 5 1.61974592185344 4.264929249339407 6 0.9628124688229082 3.042200881886014 7 0.6193704627039779 2.2831974037980847 8 0.430049950385501 1.811773155115416 9 0.30045104012774665 1.4240038151318135 >10 1.1101964584293762 9.025205883437144 >50 0.018771069952551483 0.6662308529000301 >100 0.00786112587171726 0.7751053853759541 >500 9.324892742451484E-4 0.3563902557123417 >1k 6.216595161630636E-4 0.5704126506340729 >5k 2.3312231856114886E-4 0.9279487241393384 >10k+ 4.662446371222977E-4 6.055237815791934 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 79989 2.195602372235425 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18363 0.5040423853449738 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13677 0.37541729044073446 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 13173 0.36158309329354354 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12633 0.3467607392072676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10135 0.2781936271563094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9507 0.26095577833004774 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7623 0.20924223185126262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5546 0.15223106622682703 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3876 0.10639156377482539 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.7448803863474042E-5 7 0.0 0.0 0.0 0.0 2.7448803863474042E-5 8 0.0 0.0 0.0 0.0 2.7448803863474042E-5 9 0.0 0.0 0.0 0.0 2.7448803863474042E-5 10-11 0.0 0.0 0.0 8.234641159042212E-5 2.7448803863474042E-5 12-13 0.0 0.0 0.0 9.607081352215914E-5 8.234641159042213E-5 14-15 0.0 0.0 0.0 1.372440193173702E-4 1.372440193173702E-4 16-17 0.0 0.0 0.0 1.9214162704431827E-4 1.6469282318084424E-4 18-19 0.0 0.0 0.0 2.744880386347404E-4 1.6469282318084424E-4 20-21 0.0 0.0 0.0 4.529052637473217E-4 1.921416270443183E-4 22-23 0.0 0.0 0.0 9.332593313581175E-4 1.921416270443183E-4 24-25 0.0 0.0 0.0 0.0017155502414671277 1.921416270443183E-4 26-27 0.0 0.0 0.0 0.0030605416307773557 1.921416270443183E-4 28-29 0.0 0.0 0.0 0.009016932069151221 1.921416270443183E-4 30-31 0.0 0.0 0.0 0.02426474261531105 1.921416270443183E-4 32-33 0.0 0.0 0.0 0.059563904383738674 2.1959043090779233E-4 34-35 0.0 0.0 0.0 0.1110304116277525 2.470392347712664E-4 36-37 0.0 0.0 0.0 0.18769492081843547 2.470392347712664E-4 38-39 0.0 0.0 0.0 0.2983684979959628 2.470392347712664E-4 40-41 0.0 0.0 0.0 0.4241526417003326 2.470392347712664E-4 42-43 0.0 0.0 0.0 0.5474801174589214 2.470392347712664E-4 44-45 0.0 0.0 0.0 0.6970623741129233 2.470392347712664E-4 46-47 0.0 0.0 0.0 0.8853337198124918 2.470392347712664E-4 48-49 0.0 0.0 0.0 1.0726580817787703 2.470392347712664E-4 50-51 0.0 0.0 0.0 1.2740911289308747 2.470392347712664E-4 52-53 0.0 0.0 0.0 1.4816589837464653 2.607636367030034E-4 54-55 0.0 0.0 0.0 1.7120093457687395 2.8821244056647744E-4 56-57 0.0 0.0 0.0 2.0124914016621895 3.0193684249821447E-4 58-59 0.0 0.0 0.0 2.3195748948848056 3.0193684249821447E-4 60-61 0.0 0.0 0.0 2.6314482043815977 3.293856463616885E-4 62-63 0.0 0.0 0.0 2.9513228402045923 3.842832540886366E-4 64-65 0.0 0.0 0.0 3.293513353568591 4.117320579521106E-4 66-67 0.0 0.0 0.0 3.690834789492378 4.117320579521106E-4 68-69 0.0 0.0 0.0 4.137330757537579 4.117320579521106E-4 70-71 0.0 0.0 0.0 4.621198272042899 4.3918086181558467E-4 72-73 0.0 0.0 0.0 5.078509068810309 4.3918086181558467E-4 74-75 0.0 0.0 0.0 5.554663469429993 4.3918086181558467E-4 76-77 0.0 0.0 0.0 6.042044430829838 4.3918086181558467E-4 78-79 0.0 0.0 0.0 6.675329509165978 4.3918086181558467E-4 80-81 0.0 0.0 0.0 7.325728916710997 4.529052637473217E-4 82-83 0.0 0.0 0.0 7.943409350050754 4.666296656790587E-4 84-85 0.0 0.0 0.0 8.568295094404672 4.666296656790587E-4 86-87 0.0 0.0 0.0 9.194594452157558 5.215272734060067E-4 88 0.0 0.0 0.0 9.717233402120035 6.038736849964289E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGCG 100 5.3277152E-5 28.19918 7 GTACTAG 1125 0.0 26.331884 1 CTAACGC 530 0.0 25.726828 3 CTAGTAC 1900 0.0 23.75646 3 TAGCGCG 100 0.0017554952 23.508995 4 GTGATCG 790 0.0 23.201859 8 TAACGCC 665 0.0 22.6252 4 TAGTACT 2250 0.0 22.359669 4 GAGTACT 10975 0.0 21.947271 12-13 TGATCGC 815 0.0 21.913473 9 CTGTGCG 2130 0.0 21.403135 9 AGTACTT 11670 0.0 20.620077 12-13 TAGGCAT 1945 0.0 20.298449 5 TCTAACG 675 0.0 20.201433 2 GTACTTT 12605 0.0 19.053257 14-15 CCTAGTA 2360 0.0 18.927774 2 GAGTACG 1195 0.0 18.887186 1 GTATAAG 1990 0.0 18.666718 1 CATGGGG 6480 0.0 18.647568 4 CTAGGCA 2215 0.0 18.255291 4 >>END_MODULE