FastQCFastQC Report
Mon 16 Jan 2017
ERR1146611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122835
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT361350.7053711470308921No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA121300.2367829531890057No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC90120.17591821715905354No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT88340.17244357860442508No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG65750.1283469016667529No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57100.11146171992656409No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC3252.5465852E-1120.2419999
GTACTAG16950.019.4219841
CATGGGG91200.018.97014
GTATAAG26650.016.9410081
GTATATA38750.016.7483711
CTGTGCG20700.016.5714789
GTACATG291050.016.4007471
ATGGGGG60300.016.3721565
GGCGTCG11000.016.2336658
TACATGG283350.016.2270952
TACCTGG40800.016.1321772
ACATGGG267050.015.9674513
CGACGAG7800.015.96560724-25
CGGGCGT11900.015.7961566
CTAGTAC24350.015.6389273
CGATAAT11000.015.59659666-67
TAGTACT29400.015.3511374
TACGGGC12300.015.28873354
GCATCGT16350.015.2329438
TGATCGC10550.015.143819
Produced by FastQC (version 0.11.5)