##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146611_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5122835
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25978896450891	31.0	31.0	34.0	28.0	34.0
2	31.407366428940225	33.0	31.0	34.0	28.0	34.0
3	31.44794806000974	33.0	31.0	34.0	28.0	34.0
4	34.92716044924344	37.0	35.0	37.0	32.0	37.0
5	34.95457495703063	37.0	35.0	37.0	32.0	37.0
6	35.07020155831683	37.0	35.0	37.0	32.0	37.0
7	34.947162655053305	37.0	35.0	37.0	32.0	37.0
8	34.981861996336015	37.0	35.0	37.0	32.0	37.0
9	36.57700960503315	39.0	37.0	39.0	32.0	39.0
10-11	36.58009529879452	39.0	37.0	39.0	32.0	39.0
12-13	36.564173938844405	39.0	37.0	39.0	32.0	39.0
14-15	37.88952464797324	40.0	38.0	41.0	32.0	41.0
16-17	37.90777977428513	40.0	38.0	41.0	32.0	41.0
18-19	37.92054555729396	40.0	38.0	41.0	32.0	41.0
20-21	37.99881989172012	40.0	38.0	41.0	32.0	41.0
22-23	37.99409721374981	40.0	38.0	41.0	32.0	41.0
24-25	37.99579022162533	40.0	38.0	41.0	32.0	41.0
26-27	38.02492857958533	40.0	38.0	41.0	32.0	41.0
28-29	38.00697094479912	40.0	38.0	41.0	32.0	41.0
30-31	37.98491352932507	40.0	38.0	41.0	32.0	41.0
32-33	37.933352138025136	40.0	38.0	41.0	32.0	41.0
34-35	37.876931035256845	40.0	38.0	41.0	32.0	41.0
36-37	37.81103910237203	40.0	38.0	41.0	31.0	41.0
38-39	37.75865326523302	40.0	38.0	41.0	31.0	41.0
40-41	37.72915368150643	40.0	38.0	41.0	31.0	41.0
42-43	37.58950600595178	40.0	37.5	41.0	31.0	41.0
44-45	37.47867323464449	40.0	37.0	41.0	30.5	41.0
46-47	37.35830843663713	40.0	37.0	41.0	30.0	41.0
48-49	37.245498342226526	40.0	37.0	41.0	30.0	41.0
50-51	37.12376467327174	40.0	36.0	41.0	30.0	41.0
52-53	37.038068179045396	40.0	36.0	41.0	30.0	41.0
54-55	36.93076499633504	39.5	36.0	41.0	30.0	41.0
56-57	36.72565337357147	39.0	35.0	41.0	30.0	41.0
58-59	36.53789161665367	39.0	35.0	41.0	29.0	41.0
60-61	36.30375739214712	39.0	35.0	41.0	29.0	41.0
62-63	36.095870060230325	38.5	35.0	40.0	29.0	41.0
64-65	35.82911873991647	38.0	35.0	40.0	28.0	41.0
66-67	35.56322426937428	37.0	34.5	40.0	28.0	41.0
68-69	35.28372971216133	37.0	34.0	40.0	28.0	41.0
70-71	34.943230750160794	36.0	34.0	39.0	28.0	41.0
72-73	34.58659980264834	36.0	34.0	39.0	27.5	41.0
74-75	34.26857892163226	35.0	34.0	38.5	27.0	40.0
76-77	33.97625533518062	35.0	34.0	37.0	27.0	39.5
78-79	33.636653337458654	35.0	33.0	37.0	26.0	39.0
80-81	33.350856508163936	35.0	33.0	36.5	26.0	39.0
82-83	33.06668456040454	35.0	33.0	36.0	26.0	37.5
84-85	32.85661250069542	35.0	33.0	36.0	26.0	37.0
86-87	32.67609312031327	35.0	33.0	35.0	26.0	37.0
88-89	32.48141575904748	35.0	33.0	35.0	26.0	36.0
90-91	32.296271595708234	35.0	33.0	35.0	25.0	36.0
92-93	32.11226059398751	35.0	32.0	35.0	25.0	36.0
94-95	31.983186263075034	35.0	32.0	35.0	25.0	36.0
96-97	31.89168292556758	35.0	32.0	35.0	25.0	35.0
98-99	31.81412830981283	35.0	32.0	35.0	24.0	35.0
100	30.649706461363678	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24992670761721314
1101	2	0.2814774601252239
1101	3	0.2930234079575271
1101	4	0.26337580506960023
1101	5	0.22373936506243552
1101	6	0.3191758034564529
1101	7	0.27832354159194495
1101	8	0.31451501862990483
1101	9	0.4363409054958609
1101	10-11	0.4110003622029339
1101	12-13	0.4520680002374249
1101	14-15	0.5684895211717489
1101	16-17	0.557147040523013
1101	18-19	0.5175379325855474
1101	20-21	0.5022759317465173
1101	22-23	0.5672632503508765
1101	24-25	0.5856376881282301
1101	26-27	0.5544186855317861
1101	28-29	0.5179798606402173
1101	30-31	0.5537010967518725
1101	32-33	0.5578032678562508
1101	34-35	0.5561829112219883
1101	36-37	0.5315489644826883
1101	38-39	0.5949187128588349
1101	40-41	0.5295057859245844
1101	42-43	0.567445569234934
1101	44-45	0.4910185407345722
1101	46-47	0.49738708358481176
1101	48-49	0.5701462400940613
1101	50-51	0.5009591741898234
1101	52-53	0.451233938531189
1101	54-55	0.4664367781943355
1101	56-57	0.4962943827509605
1101	58-59	0.5355533236332164
1101	60-61	0.4918698574754998
1101	62-63	0.4639343935773539
1101	64-65	0.5053789671141828
1101	66-67	0.504163301558954
1101	68-69	0.49083339978712104
1101	70-71	0.4412058220063031
1101	72-73	0.45788542596393
1101	74-75	0.45988817848456875
1101	76-77	0.42957013238331854
1101	78-79	0.43351639064167813
1101	80-81	0.3874350573131622
1101	82-83	0.413370517857075
1101	84-85	0.3475451670878513
1101	86-87	0.42766895291681806
1101	88-89	0.41656381604871484
1101	90-91	0.3602548147290108
1101	92-93	0.320602814569817
1101	94-95	0.3133734713317793
1101	96-97	0.3071499554710151
1101	98-99	0.36397329545786405
1101	100	0.3680511967404918
1102	1	0.23198609610723864
1102	2	0.24078239220035513
1102	3	0.20567246399936678
1102	4	0.18030146360662513
1102	5	0.14528970458954404
1102	6	0.20465159982131098
1102	7	0.16536545781284673
1102	8	0.2765097855616716
1102	9	0.33710510681952144
1102	10-11	0.3671128892837032
1102	12-13	0.3618288226814954
1102	14-15	0.45822609525122715
1102	16-17	0.37896967962745265
1102	18-19	0.3433792658233088
1102	20-21	0.37807948665456337
1102	22-23	0.4019644492669272
1102	24-25	0.38595196115792874
1102	26-27	0.4168936300922681
1102	28-29	0.4190448772623441
1102	30-31	0.4460008185402202
1102	32-33	0.4683594855558866
1102	34-35	0.4282117558852079
1102	36-37	0.42211385025032655
1102	38-39	0.4338241803538594
1102	40-41	0.4011031052689873
1102	42-43	0.4148505997148604
1102	44-45	0.3941621822899535
1102	46-47	0.32544264145555246
1102	48-49	0.2894989798981058
1102	50-51	0.3127096960797502
1102	52-53	0.2784423261093991
1102	54-55	0.3365144296368783
1102	56-57	0.30591923876934857
1102	58-59	0.3435108767709707
1102	60-61	0.37565951458116587
1102	62-63	0.25908350935107194
1102	64-65	0.30046782870235944
1102	66-67	0.2895632856674908
1102	68-69	0.29204202851543215
1102	70-71	0.23037307071150082
1102	72-73	0.2671025827376141
1102	74-75	0.3188043254838888
1102	76-77	0.24327869304507033
1102	78-79	0.1746167018586533
1102	80-81	0.22127361676916024
1102	82-83	0.2136853465321522
1102	84-85	0.1720273139238131
1102	86-87	0.1821531167455248
1102	88-89	0.22816513603989108
1102	90-91	0.24214883203958948
1102	92-93	0.20151092804816528
1102	94-95	0.22770190998491557
1102	96-97	0.2754910261663319
1102	98-99	0.22228264435815603
1102	100	0.19684602968293063
1103	1	0.12434774237135926
1103	2	0.14238954690103256
1103	3	0.1500201682230724
1103	4	0.1841646137077504
1103	5	0.20963323871225015
1103	6	0.21832295019172676
1103	7	0.20610284747334617
1103	8	0.2304231273954045
1103	9	0.3278838752399409
1103	10-11	0.3275890027199253
1103	12-13	0.3447000072280062
1103	14-15	0.4061474187142764
1103	16-17	0.4233403820034525
1103	18-19	0.3889599708162379
1103	20-21	0.3911282663965707
1103	22-23	0.409451182808823
1103	24-25	0.4136128246262203
1103	26-27	0.37438475055899545
1103	28-29	0.3240502668687384
1103	30-31	0.3965302362533194
1103	32-33	0.3580235076512466
1103	34-35	0.3984544756542405
1103	36-37	0.3429390714791083
1103	38-39	0.3738545884041713
1103	40-41	0.29605667150735826
1103	42-43	0.3727410322672995
1103	44-45	0.28161096692439
1103	46-47	0.29033856364573296
1103	48-49	0.303799242942695
1103	50-51	0.31224069355938155
1103	52-53	0.30566234532868464
1103	54-55	0.2252524615736462
1103	56-57	0.25089966501289496
1103	58-59	0.27341793841448236
1103	60-61	0.2993380695567538
1103	62-63	0.28387629587528096
1103	64-65	0.2357627259026316
1103	66-67	0.27240880346062823
1103	68-69	0.3099135016456174
1103	70-71	0.2837058662064109
1103	72-73	0.2563723354972822
1103	74-75	0.31858924159107715
1103	76-77	0.28625406298966993
1103	78-79	0.25428015790583913
1103	80-81	0.18329671577647133
1103	82-83	0.2187441266919521
1103	84-85	0.19305438723174717
1103	86-87	0.17794032018976935
1103	88-89	0.10185192846809343
1103	90-91	0.19400420061530355
1103	92-93	0.15813312647261313
1103	94-95	0.22762630991671529
1103	96-97	0.2180781846644173
1103	98-99	0.16567081559034946
1103	100	0.2949833605477252
1104	1	0.2617391284030184
1104	2	0.2534175821816511
1104	3	0.18077920989807694
1104	4	0.24506457183105823
1104	5	0.19727633878728312
1104	6	0.27701457394563533
1104	7	0.2507360198912494
1104	8	0.26043539438477126
1104	9	0.342641402881128
1104	10-11	0.3634508633159257
1104	12-13	0.38748870083704645
1104	14-15	0.5169620052224033
1104	16-17	0.4744652605867046
1104	18-19	0.4147316998262838
1104	20-21	0.4521836438808009
1104	22-23	0.4444417339116029
1104	24-25	0.5095752859810574
1104	26-27	0.46783509392023603
1104	28-29	0.44935432677647924
1104	30-31	0.4928958545028763
1104	32-33	0.5016086379130229
1104	34-35	0.47307260245970184
1104	36-37	0.5058589089928134
1104	38-39	0.49893592430615286
1104	40-41	0.47065866532729217
1104	42-43	0.5066201440078117
1104	44-45	0.4373923678319471
1104	46-47	0.4659826891274079
1104	48-49	0.45010145411882974
1104	50-51	0.471109987366809
1104	52-53	0.4766191724726738
1104	54-55	0.4197413547778268
1104	56-57	0.43557541075151107
1104	58-59	0.45764990371011294
1104	60-61	0.4740276255256788
1104	62-63	0.4747733451275522
1104	64-65	0.5295159450992912
1104	66-67	0.42445550208935146
1104	68-69	0.4607497921996
1104	70-71	0.4126032402892008
1104	72-73	0.4183965505658662
1104	74-75	0.430367297888985
1104	76-77	0.36150961540085547
1104	78-79	0.388780473461118
1104	80-81	0.31075587613498357
1104	82-83	0.2973822974738596
1104	84-85	0.36164886179768274
1104	86-87	0.28973198345222784
1104	88-89	0.30575500583262283
1104	90-91	0.3018354774123324
1104	92-93	0.35197073055750394
1104	94-95	0.30220738512542766
1104	96-97	0.33645079367702024
1104	98-99	0.3566159875156778
1104	100	0.3825811303869564
1105	1	0.11287523537324873
1105	2	0.14440888044916278
1105	3	0.12034045247343528
1105	4	0.17373857022835892
1105	5	0.095064330525517
1105	6	0.2060077079928604
1105	7	0.1337353179978109
1105	8	0.18371010879689464
1105	9	0.27097815837204564
1105	10-11	0.2603070526001545
1105	12-13	0.28463066780137325
1105	14-15	0.3195265355744539
1105	16-17	0.3055136156923979
1105	18-19	0.3114391021258669
1105	20-21	0.29159307210527174
1105	22-23	0.3052468406134494
1105	24-25	0.3600856771547427
1105	26-27	0.3044184330425779
1105	28-29	0.20445007696163486
1105	30-31	0.3248111372171323
1105	32-33	0.32610637033028667
1105	34-35	0.3581046014362812
1105	36-37	0.3432994103322571
1105	38-39	0.32521961300862756
1105	40-41	0.30949241945504014
1105	42-43	0.2967961412260465
1105	44-45	0.3099826032756283
1105	46-47	0.2872166739803248
1105	48-49	0.28760014921959254
1105	50-51	0.2918422835377399
1105	52-53	0.28383469589575583
1105	54-55	0.326624351912173
1105	56-57	0.3215808266053486
1105	58-59	0.2904063671845094
1105	60-61	0.3362007791074788
1105	62-63	0.27320613740695165
1105	64-65	0.28026434989554616
1105	66-67	0.29695043718223957
1105	68-69	0.28586144412422954
1105	70-71	0.20205613352130314
1105	72-73	0.2135323986440838
1105	74-75	0.28827814755206305
1105	76-77	0.27170633536815103
1105	78-79	0.27198343075190934
1105	80-81	0.24234382368695861
1105	82-83	0.2593067040436452
1105	84-85	0.30476077792881284
1105	86-87	0.2180613366056008
1105	88-89	0.2515500605396852
1105	90-91	0.2386803847749306
1105	92-93	0.29379195423602766
1105	94-95	0.273225553878234
1105	96-97	0.2827664130268097
1105	98-99	0.2264523066482944
1105	100	0.4292151926037917
1106	1	0.13218241218499216
1106	2	0.14563752359333293
1106	3	0.1393070967458634
1106	4	0.16052419079314717
1106	5	0.1518862582874263
1106	6	0.1547954653299115
1106	7	0.1996774124386249
1106	8	0.2097224535234119
1106	9	0.2906516395189698
1106	10-11	0.2662224420157102
1106	12-13	0.2974803672505075
1106	14-15	0.37971008557145325
1106	16-17	0.31344351560908734
1106	18-19	0.31132770263078413
1106	20-21	0.3759361380484165
1106	22-23	0.3061798522203958
1106	24-25	0.36553346274313014
1106	26-27	0.35648464775542976
1106	28-29	0.3411386821417679
1106	30-31	0.28042616996373937
1106	32-33	0.3133920817043574
1106	34-35	0.2763425082524762
1106	36-37	0.3526208238833064
1106	38-39	0.2754833773572827
1106	40-41	0.2496993575382902
1106	42-43	0.2888940821085626
1106	44-45	0.25674747427790123
1106	46-47	0.3034385312472949
1106	48-49	0.294964031532281
1106	50-51	0.30510511213426383
1106	52-53	0.24626221249302205
1106	54-55	0.2394428311900043
1106	56-57	0.2713536473173406
1106	58-59	0.25754968684554314
1106	60-61	0.29054081577566393
1106	62-63	0.30637007381501746
1106	64-65	0.3536154000680938
1106	66-67	0.3742038907979932
1106	68-69	0.32588085064949723
1106	70-71	0.2692642855831693
1106	72-73	0.2631169618320186
1106	74-75	0.26184583144785023
1106	76-77	0.264657077680269
1106	78-79	0.3179570295797518
1106	80-81	0.35916233795635577
1106	82-83	0.28706277392318214
1106	84-85	0.26311733990808506
1106	86-87	0.19651263062471713
1106	88-89	0.2288966610483385
1106	90-91	0.23123052992912818
1106	92-93	0.234595709034231
1106	94-95	0.2473169315554955
1106	96-97	0.2490042079716268
1106	98-99	0.22696673528767164
1106	100	0.2961163717233468
1107	1	0.005740870183988278
1107	2	0.09643088155757695
1107	3	0.05317728735244032
1107	4	0.05194442370649455
1107	5	0.04179530901416939
1107	6	0.020289963788052034
1107	7	0.03562881437024856
1107	8	0.15334468089493214
1107	9	0.15514911007896615
1107	10-11	0.2025239321254233
1107	12-13	0.18298563899833908
1107	14-15	0.20342267701561667
1107	16-17	0.17125298286045876
1107	18-19	0.18026877030438015
1107	20-21	0.1851949215466675
1107	22-23	0.19916471389392854
1107	24-25	0.24479159980900533
1107	26-27	0.19038548572947178
1107	28-29	0.17285226938918896
1107	30-31	0.22162156425117985
1107	32-33	0.2063439219678571
1107	34-35	0.14030452462112208
1107	36-37	0.1299932328725859
1107	38-39	0.10418907716755399
1107	40-41	0.18346510076887768
1107	42-43	0.224687462378661
1107	44-45	0.1494638522062317
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2216	58-59	-0.6640035561859321
2216	60-61	-0.7452090648107159
2216	62-63	-0.7459967111900738
2216	64-65	-0.6748287872139684
2216	66-67	-0.6699917064282701
2216	68-69	-0.7318668328757241
2216	70-71	-0.739037794301943
2216	72-73	-0.649412966297298
2216	74-75	-0.6162521248336077
2216	76-77	-0.6635604126103445
2216	78-79	-0.6199165453340001
2216	80-81	-0.6323743021607626
2216	82-83	-0.6456102994409108
2216	84-85	-0.6134347984488144
2216	86-87	-0.6645716350813586
2216	88-89	-0.5880663788899056
2216	90-91	-0.5967164666138771
2216	92-93	-0.5492197456329677
2216	94-95	-0.6988254086492383
2216	96-97	-0.6462336192077842
2216	98-99	-0.6104287783137785
2216	100	-0.6644175202822389
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1527.0
1	365.0
2	265.0
3	256.0
4	190.0
5	270.0
6	453.0
7	1252.0
8	3215.0
9	4162.0
10	4497.0
11	5751.0
12	7912.0
13	10345.0
14	12868.0
15	13993.0
16	15106.0
17	16549.0
18	18034.0
19	19853.0
20	21581.0
21	24389.0
22	27329.0
23	31557.0
24	37325.0
25	44433.0
26	49490.0
27	48850.0
28	54306.0
29	66080.0
30	77841.0
31	90945.0
32	115180.0
33	154154.0
34	223164.0
35	335614.0
36	581724.0
37	1131145.0
38	1414793.0
39	454950.0
40	1122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21656159848225	19.72931387691343	13.356701357240619	23.697423167363702
2	14.266304692827148	21.456911499036956	40.12714008829021	24.149643719845688
3	18.957207650272156	26.458757290831734	30.411224691727508	24.1728103671686
4	11.868660574553822	17.817505815260784	40.61762592015135	29.69620769003405
5	12.435363331871404	39.410624159349695	35.65597829962267	12.49803420915624
6	29.085426633016016	38.68053251809035	18.840791220195708	13.393249628697928
7	25.672350000029304	33.39352497366982	23.474602976448285	17.459522049852588
8	24.684938941443292	35.06484474010625	21.286126255736175	18.964090062714277
9	25.35838584892816	17.069298707062508	21.401027059896983	36.17128838411235
10-11	23.9924238450598	26.559744463494557	29.171390530744016	20.27644116070163
12-13	25.382163776159793	24.44691782360518	28.365275866204687	21.80564253403034
14-15	22.530803090247474	26.0255137348402	26.692488729504692	24.75119444540763
16-17	21.592710444671116	28.794044392584755	27.55328959185988	22.05995557088425
18-19	21.439889336507427	29.52124677325901	27.83300728939691	21.205856600836658
20-21	22.7675781749792	27.996810902704404	26.942519871303144	22.29309105101325
22-23	22.201573244394375	27.96510916376719	26.77403635923934	23.059281232599098
24-25	22.739359065434236	28.03945387812423	26.84127035134807	22.379916705093464
26-27	22.602576095897543	27.459571109080017	27.686946078729168	22.250906716293272
28-29	21.944210533204647	28.879833721097985	27.138394522462427	22.037561223234935
30-31	22.5476914228456	27.832774251628084	27.008867454900077	22.610666870626236
32-33	22.381580390765933	28.225515686599927	27.06070237918129	22.332201543452847
34-35	22.844035155790575	28.071012962066426	27.224100386169788	21.860851495973215
36-37	22.777979173205505	27.505079902536988	27.707885261068547	22.009055663188956
38-39	22.502961637091975	28.093903360025756	27.508961107431013	21.894173895451257
40-41	22.37729278082963	28.06116986671658	27.4518585869558	22.109678765497993
42-43	22.364353580409006	27.99877693048628	26.68103675656032	22.955832732544394
44-45	22.584363222103327	27.740433449065367	26.96469798471524	22.71050534411606
46-47	22.76423248672632	28.19942175470623	27.16376524858706	21.872580509980388
48-49	22.72929894771086	27.708991227748818	27.416706598262742	22.14500322627758
50-51	21.666020614786095	27.981330793932635	27.865520132982486	22.487128458298784
52-53	22.10310802380065	28.506718723347802	26.620908005082445	22.769265247769106
54-55	21.94042392569392	27.798752226527174	27.07698328207054	23.183840565708365
56-57	22.732740445518722	27.241717632464592	27.120734208385542	22.904807713631143
58-59	23.114354565567698	27.374873067137596	27.180973777531754	22.32979858976295
60-61	22.543010157057978	27.860063826723326	27.710735554822186	21.886190461396517
62-63	21.91061747350636	27.83229851347614	27.511413662631174	22.745670350386327
64-65	21.393362966604588	28.56102646780584	27.14955605292799	22.896054512661586
66-67	22.47403384020132	27.37812057263163	26.782019280061114	23.365826307105937
68-69	22.173451356483667	27.424780074365803	27.03188737290827	23.36988119624226
70-71	22.54967362329919	27.36473138550749	26.941631660748	23.14396333044532
72-73	22.546829169117657	26.803906459973614	28.05182782040163	22.597436550507094
74-75	22.013141844674944	28.253402825237693	27.082448930142856	22.651006399944507
76-77	21.954712373422293	27.250207634968106	27.235064277689702	23.560015713919903
78-79	22.175231427087706	27.396717006037697	27.40581336441663	23.02223820245797
80-81	22.40132005746381	27.15243072219	27.458918692463897	22.987330527882296
82-83	22.23742948011403	26.711717842487438	28.129770757426638	22.921081919971893
84-85	22.154026885767145	27.441099941926495	27.474210829497594	22.930662342808773
86-87	21.772096025098346	27.662806209011826	27.426864384938256	23.138233380951572
88-89	21.908475708567483	27.18501454763124	27.027262556973458	23.87924718682781
90-91	22.317854856391314	26.633408550487577	27.254392153265183	23.794344439855927
92-93	22.664465463918233	27.072673883000974	27.33742976656817	22.925430886512622
94-95	21.91868905244689	26.948690244288443	27.762034180452627	23.370586522812044
96-97	21.467557563809155	26.92474705275466	28.219789276000025	23.387906107436162
98-99	21.9520485575916	27.31845225998681	27.17015870087886	23.559340481542737
100	21.956728783208124	26.23569218859492	26.998925489307435	24.80865353888952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3417.0
1	2294.0
2	1185.0
3	1189.0
4	1074.5
5	884.0
6	753.0
7	714.0
8	765.0
9	820.0
10	734.5
11	634.5
12	658.5
13	683.0
14	725.5
15	846.0
16	1012.0
17	1252.0
18	1612.5
19	2121.5
20	2976.0
21	4312.0
22	6368.5
23	9791.5
24	14142.0
25	19129.5
26	25663.5
27	32842.5
28	40795.0
29	50639.5
30	61349.5
31	72491.5
32	85390.0
33	96749.0
34	106426.0
35	116185.5
36	127773.5
37	141952.5
38	149961.0
39	152044.5
40	154774.0
41	156456.0
42	158906.0
43	166521.5
44	179300.0
45	192351.5
46	198308.0
47	222078.5
48	278866.0
49	283205.5
50	247546.0
51	220273.5
52	186876.0
53	169289.0
54	155578.5
55	137835.0
56	122602.0
57	106779.0
58	92212.0
59	81454.5
60	66962.5
61	51226.0
62	38890.5
63	30279.5
64	22673.5
65	15765.0
66	11254.5
67	8608.5
68	6781.0
69	4978.5
70	3591.0
71	2786.5
72	2207.0
73	1752.5
74	1382.0
75	1002.0
76	771.0
77	581.5
78	386.5
79	229.0
80	153.5
81	117.0
82	90.5
83	68.0
84	54.5
85	50.0
86	47.0
87	44.0
88	34.5
89	31.0
90	28.5
91	20.5
92	18.0
93	13.0
94	13.0
95	17.5
96	13.5
97	10.0
98	8.0
99	9.5
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09233168743478953
2	0.06105994044313354
3	0.07624684378864438
4	0.07761327468091399
5	0.07866737851209339
6	0.08512864458839686
7	0.08167352647508655
8	0.08245434412781205
9	0.06953181197520514
10-11	0.07572955209371374
12-13	0.06701367504516542
14-15	0.07471448914517059
16-17	0.0760223587134858
18-19	0.07599307805150858
20-21	0.06867291255720709
22-23	0.07161073897558676
24-25	0.07407031458167207
26-27	0.06906332138356984
28-29	0.07740831004707355
30-31	0.08146856184124611
32-33	0.08289355405747013
34-35	0.08512864458839686
36-37	0.08550929319410053
38-39	0.08718805114746035
40-41	0.08333276398712822
42-43	0.08911081461729686
44-45	0.08592898268244048
46-47	0.08288379383681106
48-49	0.09189247750513144
50-51	0.08914985549993315
52-53	0.09416660891869444
54-55	0.08663171856989343
56-57	0.09327842883871919
58-59	0.09705563423377876
60-61	0.09596248951996306
62-63	0.09673354695202949
64-65	0.09860750931857067
66-67	0.10081331918752019
68-69	0.09809997784429911
70-71	0.10012034352072632
72-73	0.09769004857661823
74-75	0.10175030037079077
76-77	0.09915408167547853
78-79	0.10545918422123687
80-81	0.10109636558663318
82-83	0.10532254113200992
84-85	0.10117444735190573
86-87	0.09853918777395719
88-89	0.09743628283948243
90-91	0.09733868063289175
92-93	0.0922145647868807
94-95	0.09184367640183609
96-97	0.0902527604344079
98-99	0.10312649148371945
100	0.24599660149116653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5122835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.9804421239664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47056856140335	40.72424595480016
2	14.835636431119692	16.906822460712462
3	5.901698025329105	10.088440882961756
4	2.888741876194565	6.5840715715033
5	1.617146182123657	4.607285221824513
6	0.9481649435283633	3.241611461321507
7	0.6104595352256859	2.434897795116543
8	0.4200284447977718	1.9146725191375238
9	0.2937495252463537	1.506418004001822
>10	0.9999152864528081	8.561329889878
>50	0.011264219940857596	0.42552585698975687
>100	0.0020025707144583145	0.2018712242726594
>500	1.1352689517265657E-4	0.041109304580693264
>1k	1.7029034273021123E-4	0.22605265906088043
>5k	2.270537903069483E-4	0.9745589741255384
>10k+	1.1352689515347416E-4	1.5610862197128907
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	36135	0.7053711470308921	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	12130	0.2367829531890057	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	9012	0.17591821715905354	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	8834	0.17244357860442508	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	6575	0.1283469016667529	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	5710	0.11146171992656409	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.952044131813732E-5	0.0
10-11	0.0	0.0	0.0	1.952044131813732E-5	0.0
12-13	0.0	0.0	0.0	1.952044131813732E-5	2.928066197720598E-5
14-15	0.0	0.0	0.0	1.952044131813732E-5	4.88011032953433E-5
16-17	0.0	0.0	0.0	1.952044131813732E-5	7.808176527254928E-5
18-19	0.0	0.0	0.0	5.856132395441196E-5	9.76022065906866E-5
20-21	0.0	0.0	0.0	9.76022065906866E-5	1.0736242724975526E-4
22-23	0.0	0.0	0.0	4.0016904702181507E-4	1.2688286856789259E-4
24-25	0.0	0.0	0.0	7.905778733845615E-4	1.3664308922696125E-4
26-27	0.0	0.0	0.0	0.0017568397186323588	1.6592375120416723E-4
28-29	0.0	0.0	0.0	0.004899630770852467	1.7568397186323588E-4
30-31	0.0	0.0	0.0	0.013576466936764505	1.7568397186323588E-4
32-33	0.0	0.0	0.0	0.03371180215642315	2.1472485449951052E-4
34-35	0.0	0.0	0.0	0.0643100939226034	2.1472485449951052E-4
36-37	0.0	0.0	0.0	0.11241822155115283	2.1472485449951052E-4
38-39	0.0	0.0	0.0	0.18540515163966825	2.3424529581764783E-4
40-41	0.0	0.0	0.0	0.2688355178333872	2.732861784539225E-4
42-43	0.0	0.0	0.0	0.35038216143990586	2.732861784539225E-4
44-45	0.0	0.0	0.0	0.45008281547229223	2.8304639911299115E-4
46-47	0.0	0.0	0.0	0.5757944575610965	3.1232706109019713E-4
48-49	0.0	0.0	0.0	0.7121447401682858	3.3184750240833447E-4
50-51	0.0	0.0	0.0	0.8545073187014611	3.3184750240833447E-4
52-53	0.0	0.0	0.0	0.999749162329062	3.416077230674031E-4
54-55	0.0	0.0	0.0	1.1585967535554045	3.5136794372647175E-4
56-57	0.0	0.0	0.0	1.3604576372262625	3.5136794372647175E-4
58-59	0.0	0.0	0.0	1.5804822915436474	3.611281643855404E-4
60-61	0.0	0.0	0.0	1.8087640925386042	3.708883850446091E-4
62-63	0.0	0.0	0.0	2.042687301074503	3.904088263627464E-4
64-65	0.0	0.0	0.0	2.289796567720803	3.904088263627464E-4
66-67	0.0	0.0	0.0	2.5758979159000828	3.904088263627464E-4
68-69	0.0	1.952044131813732E-5	0.0	2.8992735467763455	3.904088263627464E-4
70-71	0.0	1.952044131813732E-5	0.0	3.252730177723858	3.904088263627464E-4
72-73	0.0	1.952044131813732E-5	0.0	3.5996474608297944	3.904088263627464E-4
74-75	0.0	1.952044131813732E-5	0.0	3.946838030114185	3.904088263627464E-4
76-77	0.0	1.952044131813732E-5	0.0	4.327379273390613	3.904088263627464E-4
78-79	0.0	1.952044131813732E-5	0.0	4.772259891251622	3.904088263627464E-4
80-81	0.0	3.904088263627464E-5	0.0	5.243981115925068	4.2944970899902105E-4
82-83	0.0	3.904088263627464E-5	0.0	5.720806936003209	4.88011032953433E-4
84-85	0.0	3.904088263627464E-5	0.0	6.1968226577666465	4.88011032953433E-4
86-87	0.0	3.904088263627464E-5	0.0	6.686805255293212	5.17291694930639E-4
88	0.0	3.904088263627464E-5	0.0	7.085588350981439	5.46572356907845E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGC	325	2.5465852E-11	20.241999	9
GTACTAG	1695	0.0	19.421984	1
CATGGGG	9120	0.0	18.9701	4
GTATAAG	2665	0.0	16.941008	1
GTATATA	3875	0.0	16.748371	1
CTGTGCG	2070	0.0	16.571478	9
GTACATG	29105	0.0	16.400747	1
ATGGGGG	6030	0.0	16.372156	5
GGCGTCG	1100	0.0	16.233665	8
TACATGG	28335	0.0	16.227095	2
TACCTGG	4080	0.0	16.132177	2
ACATGGG	26705	0.0	15.967451	3
CGACGAG	780	0.0	15.965607	24-25
CGGGCGT	1190	0.0	15.796156	6
CTAGTAC	2435	0.0	15.638927	3
CGATAAT	1100	0.0	15.596596	66-67
TAGTACT	2940	0.0	15.351137	4
TACGGGC	1230	0.0	15.2887335	4
GCATCGT	1635	0.0	15.232943	8
TGATCGC	1055	0.0	15.14381	9
>>END_MODULE