FastQCFastQC Report
Mon 16 Jan 2017
ERR1146611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122835
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT387710.7568270303455021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142320.27781492083973036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135750.2649899908937141No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA119660.2335816008128312No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC78220.15268889199047012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74450.14532968561353235No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA71310.13920026703963723No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG63410.12377911839830874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT156350.021.31234412-13
TATTCGC2501.074477E-820.6792879
AGTACTT171100.019.07678212-13
GTACTTT175050.018.87452914-15
CATGGGG88050.018.4750884
TGATCGC10350.018.1636269
GTGATCG11000.017.5175788
GTACTAG17200.017.2215821
CTGTGCG21900.016.9537549
CTAACGC7250.016.860683
TACCTGG45050.016.6981812
ACTTTTT201350.016.5842316-17
GTATAAG27000.016.1949711
TCTAACG7300.016.1013072
TACTTTT203000.016.06742714-15
GACTCGT11150.016.0173857
CTAGTAC24700.015.989033
CGACGAG7250.015.88228924-25
CTCGTCG10700.015.8125399
GCTATTG30400.015.7691948
Produced by FastQC (version 0.11.5)