##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146611_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5122835
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34194737874634	33.0	31.0	34.0	30.0	34.0
2	32.389057816619115	34.0	31.0	34.0	30.0	34.0
3	32.50887272379454	34.0	31.0	34.0	30.0	34.0
4	36.00345472770449	37.0	35.0	37.0	35.0	37.0
5	36.04886786320465	37.0	35.0	37.0	35.0	37.0
6	36.12467979156073	37.0	36.0	37.0	35.0	37.0
7	36.09363077280451	37.0	36.0	37.0	35.0	37.0
8	36.09559667644966	37.0	36.0	37.0	35.0	37.0
9	37.872271896323035	39.0	38.0	39.0	35.0	39.0
10-11	37.82053892034391	39.0	38.0	39.0	35.0	39.0
12-13	37.799428734284824	39.0	38.0	39.0	35.0	39.0
14-15	39.13050322721696	40.0	39.0	41.0	36.0	41.0
16-17	39.08080018193051	40.0	38.5	41.0	36.0	41.0
18-19	39.19098048248675	40.0	39.0	41.0	36.0	41.0
20-21	39.25309735722505	40.0	39.0	41.0	36.0	41.0
22-23	39.21878032378556	40.0	39.0	41.0	36.0	41.0
24-25	39.21123899169112	41.0	39.0	41.0	36.0	41.0
26-27	39.12747697319941	40.0	39.0	41.0	35.5	41.0
28-29	39.0794820641305	40.0	39.0	41.0	35.0	41.0
30-31	38.96971335207947	40.0	38.5	41.0	35.0	41.0
32-33	38.87146004116861	40.0	38.0	41.0	35.0	41.0
34-35	38.80707362232045	40.0	38.0	41.0	35.0	41.0
36-37	38.74882921273084	40.0	38.0	41.0	35.0	41.0
38-39	38.68041436040786	40.0	38.0	41.0	35.0	41.0
40-41	38.61724874995974	40.0	38.0	41.0	35.0	41.0
42-43	38.537257983128484	40.0	38.0	41.0	35.0	41.0
44-45	38.416385653646856	40.0	38.0	41.0	34.0	41.0
46-47	38.21461866329874	40.0	38.0	41.0	34.0	41.0
48-49	37.9573127965277	40.0	38.0	41.0	33.0	41.0
50-51	37.85242839170108	40.0	37.0	41.0	33.0	41.0
52-53	37.79391079353522	40.0	37.0	41.0	33.0	41.0
54-55	37.65583822239053	40.0	37.0	41.0	33.0	41.0
56-57	37.50127107353643	40.0	36.5	41.0	33.0	41.0
58-59	37.33951317971397	39.5	36.0	41.0	32.5	41.0
60-61	37.11465389769532	39.0	36.0	41.0	32.0	41.0
62-63	36.89621049672691	39.0	35.0	41.0	32.0	41.0
64-65	36.64511017825091	39.0	35.0	41.0	31.0	41.0
66-67	36.36150012639486	38.0	35.0	40.0	31.0	41.0
68-69	36.053725622628875	37.0	35.0	40.0	31.0	41.0
70-71	35.706465365368985	37.0	35.0	39.5	31.0	41.0
72-73	35.362905012556524	36.0	35.0	39.0	30.5	41.0
74-75	35.04769644152115	36.0	35.0	39.0	30.0	40.5
76-77	34.72887287995807	35.0	34.0	37.5	30.0	39.5
78-79	34.4214643844668	35.0	34.0	37.0	30.0	39.0
80-81	34.13696400528223	35.0	34.0	37.0	30.0	39.0
82-83	33.86645958731835	35.0	34.0	36.0	29.5	38.0
84-85	33.64895668121265	35.0	34.0	36.0	29.0	37.0
86-87	33.47972753368008	35.0	34.0	35.5	29.0	37.0
88-89	33.271506011807915	35.0	34.0	35.0	29.0	36.5
90-91	33.16565378350074	35.0	34.0	35.0	29.0	36.0
92-93	33.04669152920209	35.0	34.0	35.0	29.0	36.0
94-95	32.96452608760579	35.0	34.0	35.0	29.0	36.0
96-97	32.909873048809885	35.0	34.0	35.0	29.0	35.5
98-99	32.86000895988256	35.0	34.0	35.0	29.0	35.0
100	32.10414467770288	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31275434814109815
1101	2	0.30892386452573106
1101	3	0.2762074315490679
1101	4	0.16800492142296974
1101	5	0.15546342188728346
1101	6	0.20203801070618255
1101	7	0.20512022288890108
1101	8	0.19067518969068686
1101	9	0.2758404108923216
1101	10-11	0.26620311914921047
1101	12-13	0.2933477536976383
1101	14-15	0.4548770757647205
1101	16-17	0.46973247689041386
1101	18-19	0.4310947797288023
1101	20-21	0.35865082797022296
1101	22-23	0.38952994166611177
1101	24-25	0.41542212767816267
1101	26-27	0.36783674721731074
1101	28-29	0.37835075822995634
1101	30-31	0.40772389876919846
1101	32-33	0.38720901011104303
1101	34-35	0.3798005693016435
1101	36-37	0.3733652246828072
1101	38-39	0.3879345094434541
1101	40-41	0.3821371735646224
1101	42-43	0.4252648324652384
1101	44-45	0.4095070587119167
1101	46-47	0.3479518801777033
1101	48-49	0.4441777929452968
1101	50-51	0.43979494747166115
1101	52-53	0.41769503851616463
1101	54-55	0.4524658530338641
1101	56-57	0.4614790314634689
1101	58-59	0.4019544963390729
1101	60-61	0.40819545849536354
1101	62-63	0.41996022788778475
1101	64-65	0.37465176982505
1101	66-67	0.28529087131015984
1101	68-69	0.27955563259636307
1101	70-71	0.2969039002885836
1101	72-73	0.3046850061260358
1101	74-75	0.29664243636614174
1101	76-77	0.2919679167470406
1101	78-79	0.3081171221307102
1101	80-81	0.31298354059465794
1101	82-83	0.32586647742441244
1101	84-85	0.2817941402320798
1101	86-87	0.2660781570852322
1101	88-89	0.3102290013825595
1101	90-91	0.3244389130875831
1101	92-93	0.32902225189405954
1101	94-95	0.3479720280434364
1101	96-97	0.29520077402737854
1101	98-99	0.32232915279801944
1101	100	0.366836548991742
1102	1	0.28708017526092533
1102	2	0.293630436532645
1102	3	0.28967438648456323
1102	4	0.20905217961860245
1102	5	0.2169294477836914
1102	6	0.23173685646402475
1102	7	0.19831038863236472
1102	8	0.22939624959536786
1102	9	0.24194317528367293
1102	10-11	0.2699697058585073
1102	12-13	0.3186412205858957
1102	14-15	0.4263731009554661
1102	16-17	0.46431559004926015
1102	18-19	0.3996462844442874
1102	20-21	0.3922197771725706
1102	22-23	0.3875551890811124
1102	24-25	0.3770786954899634
1102	26-27	0.3444726700248282
1102	28-29	0.3552769501865498
1102	30-31	0.37731989456629833
1102	32-33	0.36305102679263257
1102	34-35	0.37264720172944266
1102	36-37	0.37533115361910063
1102	38-39	0.3568991809542439
1102	40-41	0.37029709275155653
1102	42-43	0.380068492390258
1102	44-45	0.333150014323067
1102	46-47	0.4271275580234004
1102	48-49	0.49362054174524417
1102	50-51	0.4472425848396995
1102	52-53	0.40846402394659265
1102	54-55	0.4320274932768129
1102	56-57	0.4517336670740022
1102	58-59	0.36722477058896885
1102	60-61	0.4063539052220264
1102	62-63	0.3435249486287475
1102	64-65	0.33979825739609737
1102	66-67	0.32576609258205025
1102	68-69	0.31822588428705245
1102	70-71	0.35622429041831793
1102	72-73	0.36308080233531115
1102	74-75	0.3272593972192581
1102	76-77	0.29689859165059573
1102	78-79	0.3097169585487123
1102	80-81	0.3031192347540994
1102	82-83	0.24955960781382203
1102	84-85	0.2887517983868122
1102	86-87	0.3146666677833423
1102	88-89	0.3503418307344148
1102	90-91	0.2730562728940811
1102	92-93	0.28869974419975364
1102	94-95	0.31771452530271205
1102	96-97	0.3365405128407062
1102	98-99	0.36251211571035924
1102	100	0.4145350380252424
1103	1	0.20738118934374938
1103	2	0.20420501743615915
1103	3	0.1579372472319136
1103	4	0.14848158617341056
1103	5	0.11704245356724385
1103	6	0.1372648162272867
1103	7	0.14723206685874857
1103	8	0.15025944073448017
1103	9	0.16219112621089948
1103	10-11	0.1891390354122322
1103	12-13	0.1697436174241318
1103	14-15	0.2710509745523524
1103	16-17	0.37416846467725406
1103	18-19	0.3104265597183087
1103	20-21	0.2693587530775545
1103	22-23	0.2601516953016443
1103	24-25	0.27399832680048775
1103	26-27	0.2702631674205662
1103	28-29	0.32698697842903357
1103	30-31	0.328547863910579
1103	32-33	0.3258290557470005
1103	34-35	0.3293077407032641
1103	36-37	0.2843131912364498
1103	38-39	0.3156537996056912
1103	40-41	0.32384456237722503
1103	42-43	0.34238852474457104
1103	44-45	0.30995696765967296
1103	46-47	0.35145880834475207
1103	48-49	0.4240293662818573
1103	50-51	0.34530384616017074
1103	52-53	0.3332918070323956
1103	54-55	0.327924181433751
1103	56-57	0.3418479229406657
1103	58-59	0.3740531874263553
1103	60-61	0.28893174132466726
1103	62-63	0.3362264379449442
1103	64-65	0.3224711397617881
1103	66-67	0.28421639599230986
1103	68-69	0.27993939674241375
1103	70-71	0.25284027569703227
1103	72-73	0.2519785179242504
1103	74-75	0.28989909544824144
1103	76-77	0.2559802477753621
1103	78-79	0.2360931479885835
1103	80-81	0.2311959058043982
1103	82-83	0.13749087181587072
1103	84-85	0.1820910257044943
1103	86-87	0.17054977284318795
1103	88-89	0.2455169155711232
1103	90-91	0.24015106265434127
1103	92-93	0.2171656274219842
1103	94-95	0.20072976694773104
1103	96-97	0.19704517783748088
1103	98-99	0.22211305977740636
1103	100	0.23051407352816256
1104	1	0.24172560204189608
1104	2	0.21897136641621984
1104	3	0.24621360892496114
1104	4	0.15389313222059542
1104	5	0.11776002113534645
1104	6	0.16750296474863546
1104	7	0.13113624146363634
1104	8	0.16450825143249403
1104	9	0.23777563365062093
1104	10-11	0.25526293318889515
1104	12-13	0.25515740531493947
1104	14-15	0.39924751595601293
1104	16-17	0.41769655225440516
1104	18-19	0.3639250296995158
1104	20-21	0.32105308164736357
1104	22-23	0.33864545859532313
1104	24-25	0.3310266052106172
1104	26-27	0.33116406293447653
1104	28-29	0.3457471540506418
1104	30-31	0.44310066544545634
1104	32-33	0.39436054753209504
1104	34-35	0.3297689368768104
1104	36-37	0.3327590962998954
1104	38-39	0.3580483648715642
1104	40-41	0.3582227408713976
1104	42-43	0.34559862377643213
1104	44-45	0.3538471445561413
1104	46-47	0.46481819324285567
1104	48-49	0.49033892660028044
1104	50-51	0.45375469867251894
1104	52-53	0.41507247618324783
1104	54-55	0.4032997987794573
1104	56-57	0.40005456053441435
1104	58-59	0.36866696079253813
1104	60-61	0.3310473292817022
1104	62-63	0.3553093028790002
1104	64-65	0.33922989765412126
1104	66-67	0.3450120373431176
1104	68-69	0.3420794634214772
1104	70-71	0.3612576189623695
1104	72-73	0.3581913059843842
1104	74-75	0.3593063764668969
1104	76-77	0.3517310463334766
1104	78-79	0.2671154286776698
1104	80-81	0.27557692015180635
1104	82-83	0.24527632501363428
1104	84-85	0.2369638583094229
1104	86-87	0.2494287444684744
1104	88-89	0.3022298853863532
1104	90-91	0.27839003726349176
1104	92-93	0.24544214304443557
1104	94-95	0.3112512560844607
1104	96-97	0.3168849610101532
1104	98-99	0.33179662039341906
1104	100	0.4043555870252078
1105	1	0.11529329717795633
1105	2	0.12543575187225997
1105	3	0.18330366652273966
1105	4	0.0947780835720593
1105	5	0.08266711420702677
1105	6	0.12474943563675822
1105	7	0.10310110275711537
1105	8	0.1151777833731984
1105	9	0.1589036511945423
1105	10-11	0.16318431694771363
1105	12-13	0.15040827433038118
1105	14-15	0.24154761393258894
1105	16-17	0.3199556412809912
1105	18-19	0.22780081581827005
1105	20-21	0.1945550098613893
1105	22-23	0.23401568555857466
1105	24-25	0.1965371406349803
1105	26-27	0.20329668207509144
1105	28-29	0.22898333644879187
1105	30-31	0.2484660315403957
1105	32-33	0.23983955089655495
1105	34-35	0.21794239300543694
1105	36-37	0.21024807535810908
1105	38-39	0.1835539350356612
1105	40-41	0.18338869558503035
1105	42-43	0.1904991914028642
1105	44-45	0.20446114947508676
1105	46-47	0.2813300466629016
1105	48-49	0.3332562846262874
1105	50-51	0.3039896279877894
1105	52-53	0.2530381069402239
1105	54-55	0.35108638597159114
1105	56-57	0.27174294452061076
1105	58-59	0.17293070721805748
1105	60-61	0.12539219963260706
1105	62-63	0.16814563947352923
1105	64-65	0.2398085991170973
1105	66-67	0.24509786649792176
1105	68-69	0.15214834122330245
1105	70-71	0.18398728407682086
1105	72-73	0.1517926494896713
1105	74-75	0.1540805159641323
1105	76-77	0.0981370216521853
1105	78-79	0.11897808456804881
1105	80-81	0.13165757032757597
1105	82-83	0.149690338891304
1105	84-85	0.13378407982532536
1105	86-87	0.10785529980397968
1105	88-89	0.19835425870255818
1105	90-91	0.15275789661755823
1105	92-93	0.13336149021140642
1105	94-95	0.14257071705763025
1105	96-97	0.1775918159163723
1105	98-99	0.1998757100046049
1105	100	0.224432738362772
1106	1	0.12980907970777622
1106	2	0.10260990821301164
1106	3	0.11473161476128269
1106	4	0.10785701588575591
1106	5	0.08935059388513622
1106	6	0.07805589624666709
1106	7	0.12299222853503977
1106	8	0.10634375622500869
1106	9	0.13004706210951156
1106	10-11	0.14885048874145212
1106	12-13	0.13740799136696324
1106	14-15	0.27206823524412727
1106	16-17	0.3157252735862457
1106	18-19	0.20097367398648203
1106	20-21	0.17897837309780584
1106	22-23	0.18395386319460272
1106	24-25	0.18551070499758282
1106	26-27	0.21296761199572245
1106	28-29	0.22103593167499014
1106	30-31	0.2124684045057208
1106	32-33	0.21764138658527798
1106	34-35	0.2189536183830043
1106	36-37	0.2781742169483934
1106	38-39	0.252789483496592
1106	40-41	0.20111313929673003
1106	42-43	0.2561733317632857
1106	44-45	0.22428409796346216
1106	46-47	0.22062989920286213
1106	48-49	0.33132720218371503
1106	50-51	0.25984398452748536
1106	52-53	0.25713398941851295
1106	54-55	0.310147688598299
1106	56-57	0.23988547482057498
1106	58-59	0.2935008236265446
1106	60-61	0.31936941986964484
1106	62-63	0.3027072094100305
1106	64-65	0.3102648323190351
1106	66-67	0.233548263404451
1106	68-69	0.21621600031827626
1106	70-71	0.26370961221446976
1106	72-73	0.297065095757425
1106	74-75	0.27265159402358563
1106	76-77	0.23021092361986462
1106	78-79	0.1560017103900293
1106	80-81	0.19758059908420478
1106	82-83	0.14621642785267852
1106	84-85	0.14666086688384894
1106	86-87	0.1430223490456939
1106	88-89	0.24716392569592927
1106	90-91	0.18749837726481644
1106	92-93	0.18225667600908224
1106	94-95	0.20270176155784014
1106	96-97	0.17568682647309686
1106	98-99	0.2154824289876558
1106	100	0.2572846091764518
1107	1	0.07392915622646967
1107	2	0.04891179457964512
1107	3	0.09281677011105671
1107	4	0.0704242253894165
1107	5	0.053918717141506534
1107	6	0.04281495760587006
1107	7	0.021593861480944554
1107	8	0.02757316387689457
1107	9	0.0933221631703347
1107	10-11	0.06433853599696704
1107	12-13	0.08647771920143299
1107	14-15	0.16754028701609514
1107	16-17	0.19921707032445823
1107	18-19	0.15846728161007206
1107	20-21	0.131907652084287
1107	22-23	0.11531725729520304
1107	24-25	0.11166941752823334
1107	26-27	0.09544195567222857
1107	28-29	0.11297665198959805
1107	30-31	0.15965417123705805
1107	32-33	0.06192550297215149
1107	34-35	0.05268799346090702
1107	36-37	0.06554235983391266
1107	38-39	0.09012567652776937
1107	40-41	0.07903052838874913
1107	42-43	0.08894244072842383
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2216	50-51	-0.5393189536030221
2216	52-53	-0.5976665003745154
2216	54-55	-0.5723230728118338
2216	56-57	-0.5415108629071312
2216	58-59	-0.6238495498414736
2216	60-61	-0.6193260810867827
2216	62-63	-0.6232344270742303
2216	64-65	-0.5726047494099049
2216	66-67	-0.5496666152710574
2216	68-69	-0.5682783870962922
2216	70-71	-0.5669051464537844
2216	72-73	-0.5414792628010971
2216	74-75	-0.5248125904006926
2216	76-77	-0.5618918113650935
2216	78-79	-0.5297396059586248
2216	80-81	-0.5544064867261369
2216	82-83	-0.5322480263264069
2216	84-85	-0.45862198917278363
2216	86-87	-0.4433070975434745
2216	88-89	-0.41724280759660815
2216	90-91	-0.4689666549484244
2216	92-93	-0.4787035846824921
2216	94-95	-0.4688587048248891
2216	96-97	-0.47429819545143914
2216	98-99	-0.4410574313326663
2216	100	-0.5010047468169496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	46.0
8	174.0
9	356.0
10	418.0
11	267.0
12	256.0
13	353.0
14	603.0
15	1130.0
16	1986.0
17	3449.0
18	5823.0
19	8713.0
20	12169.0
21	18185.0
22	26048.0
23	30704.0
24	30541.0
25	28035.0
26	27683.0
27	32387.0
28	40122.0
29	50506.0
30	63146.0
31	80370.0
32	102198.0
33	134311.0
34	191408.0
35	294684.0
36	524713.0
37	1173026.0
38	1659773.0
39	577294.0
40	1957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96279305834814	19.661077166338064	13.650848185527165	23.725281589786636
2	14.593959265135803	21.698491104670047	39.490634604332726	24.216915025861425
3	18.914754195003106	26.3150322839701	30.883452691397963	23.88676082962883
4	11.858255364708796	18.15153717944261	40.004593550826506	29.985613905022085
5	12.082980167949904	39.08723845427369	35.83090070544582	12.998880672330587
6	28.855578432174443	38.89442747297346	18.38966519080265	13.86032890404945
7	25.269216218991286	33.502471483557734	23.503513875327602	17.72479842212338
8	24.90515969518363	34.63360624280914	21.036413133230084	19.424820928777148
9	25.458315740528725	16.227446805312606	21.525277126933474	36.78896032722519
10-11	24.094362704669628	26.17780136717427	29.11660724962711	20.61122867852899
12-13	25.85526373580254	24.175510239935505	28.542730734056438	21.426495290205523
14-15	22.705539815026196	25.493967352247545	27.48816646100249	24.312326371723774
16-17	21.193300804400074	28.3147439771501	28.29792708084447	22.19402813760535
18-19	20.97207264660704	28.41131469011153	29.598237457560057	21.018375205721373
20-21	22.219469833280385	26.852970213197903	29.05304644070725	21.87451351281447
22-23	21.603438330533777	26.883590824221354	28.870156856506213	22.64281398873866
24-25	22.13826914198876	27.013880009799262	28.943475634097137	21.90437521411484
26-27	22.081364529159906	26.426420828207576	29.764854215118152	21.727360427514366
28-29	21.347566469305228	27.81674871596965	29.281582295894975	21.554102518830152
30-31	22.06159041302687	26.683677458137613	29.092308708873897	22.162423419961616
32-33	21.917881621543117	27.112750701566167	29.145184374868236	21.82418330202248
34-35	22.334926886582284	27.01952641982842	29.25850435945689	21.387042334132403
36-37	22.296520304292567	26.448910202616403	29.636531192798987	21.618038300292046
38-39	22.0612587225299	27.00518876699584	29.437814928055907	21.495737582418354
40-41	21.949486467912944	27.055428905118497	29.23150215248266	21.763582474485904
42-43	22.027012386696036	27.007750591225367	28.425510483941018	22.539726538137575
44-45	22.32019686066888	26.783176267873245	28.51744872102445	22.379178150433425
46-47	22.59196323910491	27.321248878794652	28.487429323802154	21.599358558298288
48-49	22.69563450365628	27.05233688169686	28.403699048436305	21.84832956621056
50-51	21.570614708457327	27.392898658652875	28.737456896425513	22.29902973646428
52-53	21.985913620639877	27.9263222353465	27.455439102791217	22.632325041222405
54-55	21.870664620138005	27.281677419531153	27.827148362201214	23.020509598129625
56-57	22.61710939905745	26.712455182221145	27.921475005746338	22.748960412975062
58-59	22.922151751773715	26.873940946477738	28.008165857163675	22.195741444584876
60-61	22.333947092436222	27.351337475922605	28.572387867773546	21.74232756386763
62-63	21.757153352188162	27.26554906076735	28.347139679069684	22.63015790797481
64-65	21.252288778446246	28.05688078050761	27.94563345969603	22.745196981350116
66-67	22.295286934047528	26.873836081190287	27.627645536212757	23.20323144854942
68-69	21.984229290487903	26.89874304191061	27.88215528670339	23.234872380898093
70-71	22.361153569405353	26.864582833106304	27.758442172974508	23.015821424513838
72-73	22.36069973351907	26.27047921864535	28.815400415367463	22.553420632468118
74-75	21.825480873874252	27.741466666536528	27.73828475385563	22.694767705733593
76-77	21.85379113786599	26.754430700981676	27.812664862583	23.57911329856934
78-79	21.918858993082555	26.958297102145938	28.06068821898919	23.062155685782322
80-81	22.141231144417205	26.73436615014114	28.05541476303961	23.06898794240205
82-83	21.886363700692694	26.348125251998	28.699237927789024	23.06627311952028
84-85	21.805179012575227	27.0193480707514	28.021200471889408	23.15427244478397
86-87	21.424279720115912	27.21048013453488	27.947347513632586	23.41789263171662
88-89	21.487125780939657	26.831637169653135	27.549189072066543	24.13204797734067
90-91	21.812506514948563	26.270595533644077	27.790164584681058	24.12673336672631
92-93	22.109634713625088	26.75858851455153	27.81634370064984	23.31543307117355
94-95	21.31323129141815	26.641380510516697	28.276668196745568	23.768720001319583
96-97	20.787457677077935	26.59549485975972	28.756992005372044	23.860055457790295
98-99	21.233649909591005	26.989578613856285	27.687073807431865	24.089697669120845
100	21.158555750223716	25.983927733206386	27.6131888773616	25.244327639208297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	633.0
1	394.0
2	538.0
3	913.0
4	583.0
5	355.5
6	755.0
7	1927.0
8	3597.0
9	4368.0
10	3227.5
11	1978.0
12	1964.0
13	2220.5
14	2538.0
15	2900.0
16	3440.0
17	4055.0
18	4751.0
19	5671.5
20	6925.0
21	8426.0
22	10350.0
23	13691.5
24	17877.0
25	22522.0
26	28594.0
27	35258.5
28	42663.5
29	52100.0
30	62665.5
31	73082.5
32	85210.5
33	95945.5
34	104623.0
35	113541.0
36	124920.5
37	139247.5
38	147222.0
39	149708.5
40	152554.0
41	154375.5
42	157184.0
43	164816.0
44	177197.5
45	190329.0
46	195872.5
47	221702.0
48	281501.0
49	283908.0
50	247118.5
51	218251.0
52	182589.0
53	165796.0
54	153185.0
55	135749.0
56	120590.5
57	104887.5
58	90903.0
59	80963.5
60	66400.5
61	50350.0
62	38018.5
63	29187.5
64	21441.0
65	14746.5
66	10456.5
67	8013.0
68	6217.5
69	4463.0
70	3266.0
71	2464.0
72	1893.0
73	1558.5
74	1194.5
75	833.5
76	631.5
77	483.0
78	294.0
79	138.5
80	77.5
81	42.0
82	26.5
83	23.5
84	24.0
85	18.5
86	11.5
87	6.5
88	5.0
89	4.0
90	1.5
91	0.5
92	2.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002635259577948538
2	0.0028695048737661863
3	2.732861784539225E-4
4	0.051006913164292816
5	0.0022838916342220665
6	0.0036308020851735416
7	1.952044131813732E-5
8	2.5376573713578517E-4
9	3.904088263627464E-5
10-11	1.1712264790882392E-4
12-13	0.0
14-15	2.928066197720598E-5
16-17	2.1472485449951052E-4
18-19	1.5616353054509857E-4
20-21	2.440055164767165E-4
22-23	0.0
24-25	0.0
26-27	2.5376573713578517E-4
28-29	9.76022065906866E-6
30-31	1.659237512041672E-4
32-33	2.1472485449951052E-4
34-35	1.2688286856789259E-4
36-37	3.904088263627464E-5
38-39	7.417767700892182E-4
40-41	5.758530188850509E-4
42-43	0.0
44-45	4.6849059163529567E-4
46-47	0.0
48-49	7.808176527254928E-5
50-51	0.0
52-53	2.732861784539225E-4
54-55	2.732861784539225E-4
56-57	1.464033098860299E-4
58-59	8.100983147026988E-4
60-61	6.344143428394629E-4
62-63	1.8544419252230454E-4
64-65	2.928066197720598E-4
66-67	0.0010736242724975525
68-69	0.002157008765654174
70-71	0.003142791052220109
72-73	0.0027719026671754994
74-75	0.0025376573713578516
76-77	0.0038552871603321206
78-79	0.001200507141065445
80-81	9.4674140392966E-4
82-83	5.17291694930639E-4
84-85	4.7825081229436436E-4
86-87	0.0
88-89	0.0
90-91	1.952044131813732E-5
92-93	9.76022065906866E-5
94-95	1.0736242724975526E-4
96-97	3.904088263627464E-4
98-99	7.808176527254928E-5
100	0.06992222080156787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5122835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.16410760415565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.74819273328161	37.77848616400853
2	15.559899228396592	16.85576112233383
3	6.265937845839968	10.18166795169082
4	3.1248875876740736	6.770269901986755
5	1.6998740683288982	4.603608097524014
6	1.0523397090086435	3.419942474092201
7	0.6621673376670777	2.5105992050549806
8	0.4655418093681219	2.017252532547862
9	0.3208526784351533	1.5640829099858877
>10	1.077327571768822	8.87987893739246
>50	0.015056182270653171	0.534578059470773
>100	0.006026284735124813	0.6310326258420248
>500	8.89201512178759E-4	0.33980465327478715
>1k	5.335209069039613E-4	0.4683351289573325
>5k	2.3712040306842725E-4	0.9227672611480036
>10k+	2.3712040306842725E-4	2.521932974689752
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	38771	0.7568270303455021	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14232	0.27781492083973036	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13575	0.2649899908937141	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	11966	0.2335816008128312	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	7822	0.15268889199047012	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7445	0.14532968561353235	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	7131	0.13920026703963723	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	6341	0.12377911839830874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.904088263627464E-5
7	0.0	0.0	0.0	0.0	3.904088263627464E-5
8	0.0	0.0	0.0	0.0	3.904088263627464E-5
9	0.0	0.0	0.0	1.952044131813732E-5	5.856132395441196E-5
10-11	0.0	0.0	0.0	1.952044131813732E-5	5.856132395441196E-5
12-13	0.0	0.0	0.0	1.952044131813732E-5	8.784198593161794E-5
14-15	0.0	0.0	0.0	1.952044131813732E-5	1.464033098860299E-4
16-17	0.0	0.0	0.0	1.952044131813732E-5	1.952044131813732E-4
18-19	0.0	0.0	0.0	5.856132395441196E-5	1.952044131813732E-4
20-21	0.0	0.0	0.0	9.76022065906866E-5	1.952044131813732E-4
22-23	0.0	0.0	0.0	3.806486057036778E-4	1.952044131813732E-4
24-25	0.0	0.0	0.0	7.710574320664242E-4	1.952044131813732E-4
26-27	0.0	0.0	0.0	0.0017568397186323588	1.952044131813732E-4
28-29	0.0	0.0	0.0	0.004928911432829673	1.952044131813732E-4
30-31	0.0	0.0	0.0	0.013615507819400782	1.952044131813732E-4
32-33	0.0	0.0	0.0	0.03391676679026359	1.952044131813732E-4
34-35	0.0	0.0	0.0	0.0646224209836936	2.1472485449951052E-4
36-37	0.0	0.0	0.0	0.11286719170146998	2.1472485449951052E-4
38-39	0.0	0.0	0.0	0.1859517239965761	2.244850751585792E-4
40-41	0.0	0.0	0.0	0.26944065151424945	2.3424529581764783E-4
42-43	0.0	0.0	0.0	0.35131914262317643	2.3424529581764783E-4
44-45	0.0	0.0	0.0	0.4511661999654488	2.440055164767165E-4
46-47	0.0	0.0	0.0	0.5769559238195257	2.5376573713578517E-4
48-49	0.0	0.0	0.0	0.7137746970183502	2.928066197720598E-4
50-51	0.0	0.0	0.0	0.8571132976174325	2.928066197720598E-4
52-53	0.0	0.0	0.0	1.0029700351465545	3.220872817492658E-4
54-55	0.0	0.0	0.0	1.1633011799130755	3.416077230674031E-4
56-57	0.0	0.0	0.0	1.366909143081907	3.5136794372647175E-4
58-59	0.0	0.0	0.0	1.5887199177799012	3.5136794372647175E-4
60-61	0.0	0.0	0.0	1.8196467385734656	3.806486057036778E-4
62-63	0.0	0.0	0.0	2.055512231020519	3.904088263627464E-4
64-65	0.0	0.0	0.0	2.3052957981274043	3.904088263627464E-4
66-67	0.0	0.0	0.0	2.5939055230160646	4.0016904702181507E-4
68-69	0.0	0.0	0.0	2.9211657217146367	4.099292676808837E-4
70-71	0.0	0.0	0.0	3.2787997271042304	4.099292676808837E-4
72-73	0.0	0.0	0.0	3.629884624431589	4.58730370976227E-4
74-75	0.0	0.0	0.0	3.980862159331698	4.6849059163529567E-4
76-77	0.0	0.0	0.0	4.364624275425619	4.6849059163529567E-4
78-79	0.0	0.0	0.0	4.811769264479532	4.6849059163529567E-4
80-81	0.0	0.0	0.0	5.286008626083019	4.6849059163529567E-4
82-83	0.0	0.0	0.0	5.7653525830911985	4.88011032953433E-4
84-85	0.0	0.0	0.0	6.243330109207109	4.88011032953433E-4
86-87	0.0	0.0	0.0	6.735791802781077	5.075314742715703E-4
88	0.0	0.0	0.0	7.13546307854928	5.270519155897076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	15635	0.0	21.312344	12-13
TATTCGC	250	1.074477E-8	20.679287	9
AGTACTT	17110	0.0	19.076782	12-13
GTACTTT	17505	0.0	18.874529	14-15
CATGGGG	8805	0.0	18.475088	4
TGATCGC	1035	0.0	18.163626	9
GTGATCG	1100	0.0	17.517578	8
GTACTAG	1720	0.0	17.221582	1
CTGTGCG	2190	0.0	16.953754	9
CTAACGC	725	0.0	16.86068	3
TACCTGG	4505	0.0	16.698181	2
ACTTTTT	20135	0.0	16.58423	16-17
GTATAAG	2700	0.0	16.194971	1
TCTAACG	730	0.0	16.101307	2
TACTTTT	20300	0.0	16.067427	14-15
GACTCGT	1115	0.0	16.017385	7
CTAGTAC	2470	0.0	15.98903	3
CGACGAG	725	0.0	15.882289	24-25
CTCGTCG	1070	0.0	15.812539	9
GCTATTG	3040	0.0	15.769194	8
>>END_MODULE