FastQCFastQC Report
Mon 16 Jan 2017
ERR1146610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112102
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.041.1750761
GTATCAA48450.037.171111
CAACGCA50800.033.6815035
ATCAACG52300.032.716553
AACGCAG58000.029.9784266
TCAACGC60550.028.6461354
TATCAAC62100.028.1591222
ACGCAGA63850.026.7897457
CGCAGAG65800.025.9949878
GTACATG106450.024.7809351
GTACTAG11950.024.3963181
TACATGG106550.023.6030482
ACATGGG105300.023.1243293
CTAGTAC18300.022.0909883
CATGGGG83700.021.6778244
TAGTACT19650.021.2902764
CGGGCGT9300.021.2227386
GTATAAG16400.021.2172161
GCAGAGT78050.021.071839
CTGTGCG18650.020.912529
Produced by FastQC (version 0.11.5)