##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146610_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3112102
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155164258755015	31.0	31.0	34.0	28.0	34.0
2	31.24590421522174	31.0	31.0	34.0	28.0	34.0
3	31.43164427129959	33.0	31.0	34.0	28.0	34.0
4	34.922824187639094	37.0	35.0	37.0	32.0	37.0
5	34.89794357639949	37.0	35.0	37.0	32.0	37.0
6	34.99060731299938	37.0	35.0	37.0	32.0	37.0
7	34.837635463105	37.0	35.0	37.0	32.0	37.0
8	34.826917626735884	37.0	35.0	37.0	32.0	37.0
9	36.414061942699824	39.0	37.0	39.0	32.0	39.0
10-11	36.42908619319033	39.0	37.0	39.0	32.0	39.0
12-13	36.39353321324301	39.0	37.0	39.0	32.0	39.0
14-15	37.71208752155296	40.0	38.0	41.0	32.0	41.0
16-17	37.72531330913961	40.0	38.0	41.0	32.0	41.0
18-19	37.736046408504606	40.0	38.0	41.0	32.0	41.0
20-21	37.849600205905844	40.0	38.0	41.0	32.0	41.0
22-23	37.851535553783265	40.0	38.0	41.0	32.0	41.0
24-25	37.868060397763315	40.0	38.0	41.0	32.0	41.0
26-27	37.93649356608492	40.0	38.0	41.0	32.0	41.0
28-29	37.920264824224915	40.0	38.0	41.0	32.0	41.0
30-31	37.891264328739865	40.0	38.0	41.0	31.5	41.0
32-33	37.86298039074555	40.0	38.0	41.0	31.0	41.0
34-35	37.813444578616	40.0	38.0	41.0	31.0	41.0
36-37	37.740960932514426	40.0	38.0	41.0	31.0	41.0
38-39	37.68539173844559	40.0	38.0	41.0	31.0	41.0
40-41	37.632356844345075	40.0	37.0	41.0	31.0	41.0
42-43	37.46541903189548	40.0	37.0	41.0	30.0	41.0
44-45	37.33548289869677	40.0	37.0	41.0	30.0	41.0
46-47	37.22163396315416	40.0	36.5	41.0	30.0	41.0
48-49	37.10834847315416	40.0	36.0	41.0	30.0	41.0
50-51	36.977551667651	40.0	36.0	41.0	30.0	41.0
52-53	36.84848841715342	39.0	35.0	41.0	30.0	41.0
54-55	36.73142605865746	39.0	35.0	41.0	30.0	41.0
56-57	36.542960031515676	39.0	35.0	41.0	29.5	41.0
58-59	36.348251921048856	39.0	35.0	41.0	29.0	41.0
60-61	36.12483170538755	38.0	35.0	40.0	29.0	41.0
62-63	35.886723507134406	38.0	35.0	40.0	29.0	41.0
64-65	35.59090511814844	37.0	34.0	40.0	28.0	41.0
66-67	35.35486224423235	37.0	34.0	40.0	28.0	41.0
68-69	35.10343732306974	36.0	34.0	39.5	28.0	41.0
70-71	34.8290456096876	36.0	34.0	39.0	28.0	41.0
72-73	34.49502956522633	35.0	34.0	39.0	28.0	40.5
74-75	34.172834148752194	35.0	34.0	37.5	27.5	40.0
76-77	33.90831100651585	35.0	33.5	37.0	27.0	39.0
78-79	33.61024172729557	35.0	33.0	37.0	27.0	39.0
80-81	33.3757722272599	35.0	33.0	36.0	27.0	39.0
82-83	33.1128868848129	35.0	33.0	36.0	26.0	37.0
84-85	32.9142974748257	35.0	33.0	35.5	26.0	37.0
86-87	32.711559100569325	35.0	33.0	35.0	26.0	37.0
88-89	32.53649735773442	35.0	33.0	35.0	26.0	36.0
90-91	32.3868962199825	35.0	33.0	35.0	25.5	36.0
92-93	32.23524630619434	35.0	33.0	35.0	25.0	36.0
94-95	32.11652590435661	35.0	33.0	35.0	25.0	35.5
96-97	32.02533609116925	35.0	33.0	35.0	25.0	35.0
98-99	31.91523896067674	35.0	32.0	35.0	25.0	35.0
100	30.747775619179578	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20018747022889727
1101	2	0.23180143712126977
1101	3	0.25196343063570126
1101	4	0.24029664205789203
1101	5	0.15990134931271882
1101	6	0.26683296552101865
1101	7	0.2578444814294798
1101	8	0.31994130765861684
1101	9	0.4387436130544202
1101	10-11	0.4024973777386194
1101	12-13	0.44782674004686385
1101	14-15	0.4719219606264815
1101	16-17	0.4525649224355277
1101	18-19	0.4041705617670246
1101	20-21	0.45274453601861353
1101	22-23	0.3728422706519581
1101	24-25	0.46699015742108685
1101	26-27	0.4771426778697503
1101	28-29	0.44585108152217146
1101	30-31	0.5262000752260434
1101	32-33	0.5224214856500069
1101	34-35	0.498913644168276
1101	36-37	0.41228261753323636
1101	38-39	0.46465399059447776
1101	40-41	0.41060635875345497
1101	42-43	0.42069654582415694
1101	44-45	0.31878197035066336
1101	46-47	0.3216944236501931
1101	48-49	0.36208782593987365
1101	50-51	0.3196022626155042
1101	52-53	0.2422316321445166
1101	54-55	0.22279222782084673
1101	56-57	0.2459360803398809
1101	58-59	0.26460519637976176
1101	60-61	0.30223308321996
1101	62-63	0.26792714980464183
1101	64-65	0.33892062583178983
1101	66-67	0.20741109876482966
1101	68-69	0.20946468968786291
1101	70-71	0.258490647213911
1101	72-73	0.20776334192727575
1101	74-75	0.20666213483332285
1101	76-77	0.18416277721647845
1101	78-79	0.2369974966465449
1101	80-81	0.24961602964247476
1101	82-83	0.2391066698486668
1101	84-85	0.14743965819728544
1101	86-87	0.1806992016283644
1101	88-89	0.18394231411755868
1101	90-91	0.11899211580259816
1101	92-93	0.13434756723503938
1101	94-95	0.2026973090711479
1101	96-97	0.11748505669392983
1101	98-99	0.1289085182922669
1101	100	0.2297878009623311
1102	1	0.23622365437370263
1102	2	0.3021082100319106
1102	3	0.34000470435321617
1102	4	0.3779839689875857
1102	5	0.32434535231074335
1102	6	0.4424207124146733
1102	7	0.368896206581141
1102	8	0.39137690852331986
1102	9	0.5225242248420656
1102	10-11	0.526600294881213
1102	12-13	0.5007972843445856
1102	14-15	0.6098270043610725
1102	16-17	0.5565583190060579
1102	18-19	0.5983190373212608
1102	20-21	0.5668152979760492
1102	22-23	0.5490069613702246
1102	24-25	0.6209191351624881
1102	26-27	0.536349632705786
1102	28-29	0.518024459220868
1102	30-31	0.5474159899020634
1102	32-33	0.6162276309266019
1102	34-35	0.5981278029531296
1102	36-37	0.5950702888482198
1102	38-39	0.5782994227372242
1102	40-41	0.5790147313174856
1102	42-43	0.611620654906325
1102	44-45	0.5031602473053951
1102	46-47	0.4825204973782107
1102	48-49	0.4974016743612495
1102	50-51	0.5470054674023004
1102	52-53	0.5193426831275829
1102	54-55	0.46126879243918495
1102	56-57	0.4798986175227
1102	58-59	0.5015603121253065
1102	60-61	0.5397247276097517
1102	62-63	0.45940011758609245
1102	64-65	0.5971269339107792
1102	66-67	0.4003047058025544
1102	68-69	0.4287821183918865
1102	70-71	0.47669688679258115
1102	72-73	0.4293477882478669
1102	74-75	0.46583957745433224
1102	76-77	0.5252911599185452
1102	78-79	0.4408539496893056
1102	80-81	0.4212512518146525
1102	82-83	0.42599160132267144
1102	84-85	0.4132907430994237
1102	86-87	0.4001113890885861
1102	88-89	0.3988329391086367
1102	90-91	0.40548836512544284
1102	92-93	0.4333615053347373
1102	94-95	0.46924415728646096
1102	96-97	0.378584732059565
1102	98-99	0.4782708660717425
1102	100	0.29610585815666113
1103	1	0.15352921241955997
1103	2	0.18132671485826535
1103	3	0.22821363640800385
1103	4	0.32922531359190543
1103	5	0.30184397332111956
1103	6	0.38179694980669154
1103	7	0.3441379327537675
1103	8	0.3154327808993216
1103	9	0.45083779670564184
1103	10-11	0.4300843248784929
1103	12-13	0.4420189002333714
1103	14-15	0.5334917029773081
1103	16-17	0.4975925181696965
1103	18-19	0.5518620746049976
1103	20-21	0.418971572941949
1103	22-23	0.43526474454668573
1103	24-25	0.4848555311703251
1103	26-27	0.4532366635346534
1103	28-29	0.4780077865848327
1103	30-31	0.47006778254441883
1103	32-33	0.5049855642572538
1103	34-35	0.4667895846033119
1103	36-37	0.502335306365481
1103	38-39	0.48308173448471337
1103	40-41	0.3831186828459394
1103	42-43	0.4848853802853412
1103	44-45	0.4486849125608998
1103	46-47	0.3968186656295174
1103	48-49	0.38390536263407427
1103	50-51	0.4204054419644194
1103	52-53	0.390799623762355
1103	54-55	0.3651502894235179
1103	56-57	0.4308662067396867
1103	58-59	0.42762496609275047
1103	60-61	0.41443115407680864
1103	62-63	0.46958244894195644
1103	64-65	0.4245885849709694
1103	66-67	0.44867806663765464
1103	68-69	0.44323509451427867
1103	70-71	0.3977096801508253
1103	72-73	0.3792983588117238
1103	74-75	0.35117429875710116
1103	76-77	0.37429165741473014
1103	78-79	0.3907913817610904
1103	80-81	0.3025075754040074
1103	82-83	0.2999362436664157
1103	84-85	0.32371694805371476
1103	86-87	0.3059541924409146
1103	88-89	0.28663609601226625
1103	90-91	0.28219791479976664
1103	92-93	0.25279700063822474
1103	94-95	0.3014007767237388
1103	96-97	0.3655917846696184
1103	98-99	0.3570214639283371
1103	100	0.275650109260404
1104	1	0.21691148232356738
1104	2	0.2507679361628412
1104	3	0.20358955448578442
1104	4	0.1489578067716053
1104	5	0.1598885167693851
1104	6	0.16874902464696362
1104	7	0.25064783072329533
1104	8	0.2914506553644003
1104	9	0.3248668215110655
1104	10-11	0.3333259590207476
1104	12-13	0.33989863437086854
1104	14-15	0.4122378015991828
1104	16-17	0.45082450874640756
1104	18-19	0.3877400941010265
1104	20-21	0.3900808208369142
1104	22-23	0.41975403889622953
1104	24-25	0.48197937006435154
1104	26-27	0.4274354202644659
1104	28-29	0.40928916049043096
1104	30-31	0.4022409147952004
1104	32-33	0.4263707008597777
1104	34-35	0.41402195885432747
1104	36-37	0.505483374653295
1104	38-39	0.4125570728109764
1104	40-41	0.4837233835282859
1104	42-43	0.4200581267934922
1104	44-45	0.3185269235519783
1104	46-47	0.36372381017441313
1104	48-49	0.29577966845248227
1104	50-51	0.33802979483721174
1104	52-53	0.3639471025775336
1104	54-55	0.311540493422406
1104	56-57	0.3674999663548064
1104	58-59	0.2928841117090428
1104	60-61	0.36617925070068225
1104	62-63	0.3482957646117981
1104	64-65	0.32978465701487636
1104	66-67	0.3165599001250783
1104	68-69	0.27008642641219893
1104	70-71	0.28364323417444126
1104	72-73	0.2501700854287918
1104	74-75	0.2647618757763581
1104	76-77	0.21030587611527807
1104	78-79	0.2005915712196682
1104	80-81	0.22457171726628644
1104	82-83	0.24037709163832943
1104	84-85	0.2171287898351224
1104	86-87	0.1252810612243067
1104	88-89	0.1829137055662713
1104	90-91	0.21309636417647226
1104	92-93	0.1465360772965525
1104	94-95	0.22643911829935348
1104	96-97	0.1666605657849871
1104	98-99	0.21829921309428713
1104	100	0.23115206072509054
1105	1	0.2355019184688274
1105	2	0.17743536651234493
1105	3	0.15344044915520172
1105	4	0.3037805762000332
1105	5	0.21367410008456744
1105	6	0.3142798703208882
1105	7	0.2615917052756558
1105	8	0.27831695770942844
1105	9	0.3254599485152667
1105	10-11	0.41396742265634856
1105	12-13	0.32875418076721985
1105	14-15	0.4749853527603207
1105	16-17	0.38966818811043424
1105	18-19	0.40426783787495424
1105	20-21	0.4253049430321525
1105	22-23	0.3817979536542424
1105	24-25	0.43560749994733783
1105	26-27	0.3610825373991915
1105	28-29	0.3254484647304494
1105	30-31	0.4269383429060625
1105	32-33	0.44803473581549014
1105	34-35	0.4164140830381413
1105	36-37	0.4358470299388415
1105	38-39	0.4487781754641773
1105	40-41	0.46979395830444304
1105	42-43	0.47389626945693664
1105	44-45	0.4575791931499964
1105	46-47	0.4192067160325337
1105	48-49	0.4573071239735711
1105	50-51	0.3788741734951202
1105	52-53	0.3943119557969439
1105	54-55	0.33775712628487753
1105	56-57	0.3648753681305976
1105	58-59	0.38127935201831065
1105	60-61	0.3750582616265916
1105	62-63	0.3981996678546764
1105	64-65	0.47145336391265147
1105	66-67	0.3631248622067247
1105	68-69	0.3418310598556644
1105	70-71	0.34540234015639726
1105	72-73	0.3244054479872176
1105	74-75	0.3779535234819775
1105	76-77	0.3544644136776256
1105	78-79	0.3341911453541755
1105	80-81	0.3076563670166266
1105	82-83	0.34058755364397797
1105	84-85	0.3171978207740125
1105	86-87	0.29774700059537196
1105	88-89	0.3038422840006163
1105	90-91	0.29519474000276347
1105	92-93	0.26677239969949085
1105	94-95	0.2809211504144571
1105	96-97	0.306590696292389
1105	98-99	0.3323961244464364
1105	100	0.44263066300580434
1106	1	0.08920731141035176
1106	2	0.1093768822936596
1106	3	0.1293671600184041
1106	4	0.09272712580003883
1106	5	0.04388002219042875
1106	6	0.10852256828374607
1106	7	0.0041026363385512354
1106	8	0.0637210914388433
1106	9	0.132903685262022
1106	10-11	0.12119833028926763
1106	12-13	0.20119948634341256
1106	14-15	0.19955717222720892
1106	16-17	0.14424747201726973
1106	18-19	0.22634777797725292
1106	20-21	0.23916053369371326
1106	22-23	0.21603817832210837
1106	24-25	0.22222823144660708
1106	26-27	0.23822757377434556
1106	28-29	0.14583771760030828
1106	30-31	0.19979280652530917
1106	32-33	0.245396082531542
1106	34-35	0.18455898901579815
1106	36-37	0.1625724873622758
1106	38-39	0.16482883523889313
1106	40-41	0.1524078578460859
1106	42-43	0.215412758700829
1106	44-45	0.16893210234591294
1106	46-47	0.2529793117061345
1106	48-49	0.21439856469959295
1106	50-51	0.13994612905264603
1106	52-53	0.11438726456029968
1106	54-55	0.10161544965028213
1106	56-57	0.1298874420280569
1106	58-59	0.1217469928939039
1106	60-61	0.19881213134810594
1106	62-63	0.11508323298763656
1106	64-65	0.186351783073178
1106	66-67	0.13429405944626893
1106	68-69	0.12261749340684958
1106	70-71	0.0729205539740363
1106	72-73	0.1566329768694743
1106	74-75	0.09211577103980062
1106	76-77	0.07550958477705905
1106	78-79	0.1065181030716289
1106	80-81	0.044253915088212636
1106	82-83	0.05989297755615297
1106	84-85	0.2044892330442707
1106	86-87	0.119605131285347
1106	88-89	0.027427508270953638
1106	90-91	0.08768925008985917
1106	92-93	0.061156708157596995
1106	94-95	0.10169421945968793
1106	96-97	0.08354822561921083
1106	98-99	0.05509925963992757
1106	100	0.21427196948775062
1107	1	0.07436335340295486
1107	2	0.16570495674554664
1107	3	0.14521254322103871
1107	4	0.06281993426124188
1107	5	0.10004392700916043
1107	6	0.10501085928647313
1107	7	0.11146082261999624
1107	8	0.21771565145418492
1107	9	0.2643766303265167
1107	10-11	0.22148050402149266
1107	12-13	0.28178285873400455
1107	14-15	0.289500318446386
1107	16-17	0.28259042831246006
1107	18-19	0.31209823649940205
1107	20-21	0.3519770254326602
1107	22-23	0.3597746715190624
1107	24-25	0.3400651951196849
1107	26-27	0.25439926325686457
1107	28-29	0.2684265837420696
1107	30-31	0.27166636280597345
1107	32-33	0.34271040701129607
1107	34-35	0.2624054594117382
1107	36-37	0.19717042435462417
1107	38-39	0.20941392567043238
1107	40-41	0.19878020643715644
1107	42-43	0.21922283835275636
1107	44-45	0.18492902674837097
1107	46-47	0.21444345609197768
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2216	78-79	-0.5495339305721885
2216	80-81	-0.6054276607271234
2216	82-83	-0.6001072122645255
2216	84-85	-0.5592203185423514
2216	86-87	-0.5346061163490106
2216	88-89	-0.4724116756519976
2216	90-91	-0.5536448475880604
2216	92-93	-0.4565080229709082
2216	94-95	-0.533097917408611
2216	96-97	-0.4862338222801448
2216	98-99	-0.46107141192664614
2216	100	-0.605208339464383
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	954.0
1	223.0
2	178.0
3	157.0
4	135.0
5	154.0
6	217.0
7	501.0
8	1388.0
9	1899.0
10	2217.0
11	3151.0
12	4443.0
13	5822.0
14	7441.0
15	8386.0
16	9105.0
17	10260.0
18	11605.0
19	12801.0
20	14340.0
21	15624.0
22	17552.0
23	20231.0
24	24743.0
25	30296.0
26	32913.0
27	31708.0
28	33751.0
29	41024.0
30	48242.0
31	54879.0
32	69758.0
33	91950.0
34	134260.0
35	204959.0
36	375497.0
37	738642.0
38	814374.0
39	236007.0
40	315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30053009295441	20.031415774655496	13.283939038988624	24.384115093401466
2	14.87552340167765	20.606086522883057	40.4165285451049	24.10186153033439
3	17.532409482728127	26.26983366444376	30.5539244785252	25.643832374302917
4	12.151353360755031	17.69702765826833	39.68542976612236	30.46618921485428
5	13.09661323704768	38.71128324987233	35.2880530068471	12.904050506232883
6	29.8689608040201	38.72443819095477	18.39643015075377	13.010170854271358
7	26.656214775115494	32.991990635105175	23.215001841140896	17.13679274863844
8	25.339794928297586	35.86702981782303	20.765318871607157	18.02785638227223
9	26.259965799389427	15.796206034562038	20.714135502073066	37.22969266397547
10-11	24.34703920843824	26.63102121892446	28.93682205086953	20.08511752176777
12-13	24.778262952613996	24.49372827565362	28.403033450268328	22.324975321464056
14-15	22.468756190236082	25.747796597524676	25.687389540462597	26.09605767177664
16-17	22.160952592581634	28.532595675219902	26.515430460104817	22.791021272093644
18-19	22.106086899756615	28.433959500419093	27.65084197394175	21.809111625882544
20-21	23.367355879801828	27.49691112148999	26.97748113111234	22.158251867595837
22-23	23.402200393078928	27.32149380283284	26.631065326219495	22.64524047786873
24-25	22.911741288028853	27.45076443591498	27.112151353937815	22.525342922118348
26-27	22.75372699392668	27.72211436816952	26.727086373008884	22.797072264894915
28-29	22.606099799880568	27.85417811626608	26.83509007304054	22.704632010812816
30-31	22.97741616484833	27.62404225725794	26.741624524646046	22.656917053247682
32-33	22.966779538045287	27.872970011632326	26.3742913110693	22.78595913925309
34-35	23.0887161679208	27.723205491765647	26.834974468403296	22.35310387191026
36-37	23.083345380306184	27.543995035628754	26.74344353888015	22.629216045184915
38-39	22.951877316141854	27.599156399727594	26.886084213691703	22.562882070438853
40-41	22.84983558053338	27.30165545076662	27.096906984410303	22.7516019842897
42-43	22.83683472488454	27.302974360293835	26.87330344392842	22.98688747089321
44-45	22.78893837620462	27.724589231783796	26.82815715879307	22.658315233218513
46-47	22.831754669059386	27.475447690213567	26.71960237117223	22.973195269554815
48-49	23.01002379588129	27.30378368503433	26.833607175462504	22.852585343621882
50-51	22.784600869629962	27.38514244657118	26.695652187896545	23.134604495902312
52-53	22.635527942159822	27.52511241930592	26.723470539403603	23.115889099130655
54-55	22.593111220172986	27.43970918643342	26.760804362074854	23.20637523131874
56-57	22.932867265344036	27.302640624881903	26.68516915415125	23.079322955622818
58-59	22.67348340229944	27.290009858427833	26.972352979963105	23.064153759309622
60-61	22.663833438885167	27.48441086798988	26.79695161587581	23.05480407724915
62-63	22.702535360764227	27.289214464341715	26.683413610376245	23.324836564517813
64-65	22.449465676158322	27.307190534761283	26.837389545625314	23.405954243455078
66-67	22.777288233344308	27.25401705651791	26.58143178960915	23.38726292052863
68-69	22.423158281051055	27.139233796541628	26.896728518726814	23.540879403680503
70-71	22.609989964360615	26.786713714087206	26.923963948895434	23.67933237265674
72-73	22.666274686961696	27.008548877944694	26.928523262028087	23.396653173065527
74-75	22.556092172788354	27.229369047391206	26.895550919728773	23.31898786009167
76-77	22.488724976266283	27.117567904647625	26.8380383428807	23.555668776205394
78-79	22.46771627961586	27.000641733936142	26.919291101128586	23.61235088531941
80-81	22.34876002301129	27.09465395974182	27.04864102626372	23.50794499098317
82-83	22.559721379841438	26.77768883523386	27.372252853519676	23.290336931405033
84-85	22.61317620978027	26.93055176267035	26.945557113452995	23.51071491409638
86-87	22.217077649084825	27.21038175216513	27.021606831921652	23.550933766828397
88-89	22.53084821452982	26.716437017149815	26.91498877618074	23.837725992139628
90-91	22.46005438711409	26.630258489161417	27.137783637452262	23.77190348627223
92-93	22.65416332107882	26.64669257584239	27.025279377021928	23.673864726056863
94-95	22.044400231506835	26.61275239849022	27.3587170700588	23.98413029994415
96-97	22.2069613415681	26.563484459470544	27.221020970604876	24.008533228356484
98-99	22.118011449371256	26.817853865312753	27.060808017969535	24.003326667346453
100	22.614932161678112	26.373226733323886	26.704655267938822	24.30718583705918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1610.0
1	1056.0
2	505.0
3	520.5
4	501.5
5	465.5
6	446.0
7	423.0
8	429.5
9	434.5
10	387.0
11	392.0
12	407.0
13	401.5
14	424.0
15	448.0
16	522.5
17	652.0
18	836.0
19	1097.5
20	1507.0
21	2191.5
22	3433.0
23	5394.5
24	7494.5
25	10031.5
26	13804.5
27	18011.5
28	22084.0
29	27869.5
30	34873.0
31	41675.5
32	50352.0
33	57767.5
34	62618.5
35	68881.0
36	77704.5
37	86697.5
38	91548.0
39	92430.5
40	92851.0
41	93340.5
42	94107.5
43	98137.5
44	103780.5
45	113286.5
46	120734.5
47	123034.0
48	125477.5
49	124203.5
50	121420.5
51	121137.0
52	119579.5
53	117252.5
54	113137.0
55	102585.0
56	92532.0
57	82184.0
58	71937.5
59	64868.0
60	54478.0
61	42416.0
62	33141.0
63	25966.5
64	19149.5
65	13606.5
66	10190.5
67	7580.0
68	5621.0
69	4181.5
70	3314.0
71	2557.5
72	1811.5
73	1374.0
74	1050.5
75	758.0
76	536.0
77	373.5
78	259.5
79	168.0
80	108.0
81	78.5
82	58.0
83	43.0
84	29.0
85	27.5
86	19.5
87	13.5
88	11.5
89	8.0
90	9.0
91	7.5
92	6.0
93	7.5
94	5.0
95	4.0
96	5.5
97	3.5
98	2.5
99	2.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09112811855138425
2	0.06166250334982594
3	0.07596152054142184
4	0.07708616234300804
5	0.07923904807747303
6	0.08762566265501581
7	0.08409107413574492
8	0.08524784856023357
9	0.07120589235185736
10-11	0.0771664939002642
12-13	0.06786409957000125
14-15	0.07602578578722677
16-17	0.07843573250491148
18-19	0.07761635062089867
20-21	0.0692297360433559
22-23	0.07371223693824945
24-25	0.07576872480400706
26-27	0.0703543778449421
28-29	0.0790141197171558
30-31	0.0828057692196464
32-33	0.0855691747892582
34-35	0.08738466798324733
36-37	0.08822011617871137
38-39	0.09027660404446898
40-41	0.08601903150989267
42-43	0.09196356674684827
44-45	0.08918409486578524
46-47	0.0857780368381242
48-49	0.09480730387371622
50-51	0.09152977633766503
52-53	0.09585161411804625
54-55	0.08855750871918722
56-57	0.09544995633176546
58-59	0.09919340689990239
60-61	0.09784383673799894
62-63	0.09887208067087774
64-65	0.10044657919309843
66-67	0.10290472484513681
68-69	0.09953079944037824
70-71	0.10224600607563634
72-73	0.09953079944037824
74-75	0.10357950992608855
76-77	0.10052691075035458
78-79	0.10722656262551805
80-81	0.10261553123901467
82-83	0.1077567509034087
84-85	0.10317785213980776
86-87	0.09988425829230534
88-89	0.09925767214570731
90-91	0.0994022689487684
92-93	0.09342560108891032
94-95	0.09339346846600786
96-97	0.0914173121575064
98-99	0.10555566623458999
100	0.2462001566786693
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3112102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.413763769065596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88279825231606	39.423516721274275
2	15.617035488596834	17.620315016536313
3	6.259401628935541	10.59349214491422
4	3.113606965644453	7.026011513183334
5	1.6563282520949005	4.671985536885552
6	1.0275843050456954	3.478193894258838
7	0.6433752185040922	2.540665231409257
8	0.41252649786835854	1.8617737919380415
9	0.29485437370298806	1.497046048592054
>10	1.0556521792153446	9.32008332311086
>50	0.027525651008834133	1.0667151899120744
>100	0.009220864856443528	0.8322796948491384
>500	0.0	0.0
>1k	9.032221037447243E-5	0.0679218931360739
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.213262290246271E-5
2	0.0	0.0	0.0	0.0	3.213262290246271E-5
3	0.0	0.0	0.0	0.0	3.213262290246271E-5
4	0.0	0.0	0.0	0.0	3.213262290246271E-5
5	0.0	0.0	0.0	0.0	6.426524580492541E-5
6	0.0	0.0	0.0	0.0	6.426524580492541E-5
7	0.0	0.0	0.0	0.0	6.426524580492541E-5
8	0.0	0.0	0.0	0.0	6.426524580492541E-5
9	0.0	0.0	0.0	0.0	9.639786870738812E-5
10-11	0.0	0.0	0.0	0.0	9.639786870738812E-5
12-13	0.0	0.0	0.0	0.0	2.891936061221644E-4
14-15	0.0	0.0	0.0	0.0	3.534588519270898E-4
16-17	0.0	0.0	0.0	0.0	4.3379040918324656E-4
18-19	0.0	0.0	0.0	0.0	4.659230320857093E-4
20-21	0.0	0.0	0.0	3.213262290246271E-5	5.301882778906347E-4
22-23	0.0	0.0	0.0	8.033155725615677E-5	5.944535236955601E-4
24-25	0.0	0.0	0.0	2.5706098321970166E-4	6.105198351467915E-4
26-27	0.0	0.0	0.0	7.711829496591051E-4	6.747850809517169E-4
28-29	0.0	0.0	0.0	0.0029562013070265694	7.069177038541796E-4
30-31	0.0	0.0	0.0	0.009864715231056052	7.069177038541796E-4
32-33	0.0	0.0	0.0	0.02797144823659379	7.390503267566422E-4
34-35	0.0	0.0	0.0	0.05653734999688314	8.354481954640304E-4
36-37	0.0	0.0	0.0	0.10235847025579495	8.354481954640304E-4
38-39	0.0	0.0	0.0	0.1698209120395154	0.0010603765557812693
40-41	0.0	0.0	0.0	0.2450433822541806	0.001092509178683732
42-43	0.0	0.0	0.0	0.32633891819741123	0.001092509178683732
44-45	0.0	0.0	0.0	0.4316375234487816	0.0011567744244886575
46-47	0.0	0.0	0.0	0.5606500044021694	0.0012531722931960457
48-49	0.0	0.0	0.0	0.7022745398447737	0.0013495701619034338
50-51	0.0	0.0	0.0	0.8539887188787514	0.0014138354077083593
52-53	0.0	0.0	0.0	1.00689180496012	0.0015102332764157472
54-55	0.0	0.0	0.0	1.1789298679799054	0.0015262995878669787
56-57	0.0	0.0	0.0	1.3820723099692747	0.0016548300794768296
58-59	0.0	0.0	0.0	1.613250465441043	0.0017512279481842175
60-61	0.0	0.0	0.0	1.8553697790110992	0.001767294259635449
62-63	0.0	1.6066311451231353E-5	0.0	2.1054901156838692	0.0019279573741477626
64-65	0.0	3.213262290246271E-5	0.0	2.3637239396395104	0.001992222619952688
66-67	0.0	3.213262290246271E-5	0.0	2.662284205337743	0.001992222619952688
68-69	0.0	3.213262290246271E-5	0.0	2.9984557061433077	0.001992222619952688
70-71	0.0	3.213262290246271E-5	0.0	3.3674828138666406	0.0020082889314039193
72-73	0.0	3.213262290246271E-5	0.0	3.7426151199414415	0.0021046868001113076
74-75	0.0	3.213262290246271E-5	0.0	4.118534675277353	0.0021528857344650016
76-77	0.0	3.213262290246271E-5	0.0	4.533447168505402	0.0021528857344650016
78-79	0.0	3.213262290246271E-5	0.0	5.0016194841942845	0.002185018357367464
80-81	0.0	3.213262290246271E-5	0.0	5.508945400889816	0.002217150980269927
82-83	0.0	3.213262290246271E-5	0.0	6.023501157738403	0.002442079340587166
84-85	0.0	3.213262290246271E-5	0.0	6.529397172714776	0.0024742119634896284
86-87	0.0	6.426524580492541E-5	0.0	7.064726670269804	0.0025545435207457853
88	0.0	6.426524580492541E-5	0.0	7.4919780906924	0.002634875078001942
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2855	0.0	41.175076	1
GTATCAA	4845	0.0	37.17111	1
CAACGCA	5080	0.0	33.681503	5
ATCAACG	5230	0.0	32.71655	3
AACGCAG	5800	0.0	29.978426	6
TCAACGC	6055	0.0	28.646135	4
TATCAAC	6210	0.0	28.159122	2
ACGCAGA	6385	0.0	26.789745	7
CGCAGAG	6580	0.0	25.994987	8
GTACATG	10645	0.0	24.780935	1
GTACTAG	1195	0.0	24.396318	1
TACATGG	10655	0.0	23.603048	2
ACATGGG	10530	0.0	23.124329	3
CTAGTAC	1830	0.0	22.090988	3
CATGGGG	8370	0.0	21.677824	4
TAGTACT	1965	0.0	21.290276	4
CGGGCGT	930	0.0	21.222738	6
GTATAAG	1640	0.0	21.217216	1
GCAGAGT	7805	0.0	21.07183	9
CTGTGCG	1865	0.0	20.91252	9
>>END_MODULE