FastQCFastQC Report
Mon 16 Jan 2017
ERR1146610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112102
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97890.31454624559220745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91600.2943348257865584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.1632979895903155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.057.904591
GTATCAA107550.044.2941741
ATCAACG136050.034.3563883
CAACGCA137300.034.0940135
TATCAAC142400.033.38682
AACGCAG145450.032.603696
TCAACGC144750.032.5188454
ACGCAGA158550.029.552717
CGCAGAG161100.029.0557568
GCAGAGT176650.026.205419
GTGGTAT17150.025.775751
GTACATG103450.025.638711
TACATGG103300.025.2198832
TAACGCC5850.024.9187054
ACATGGG101300.024.6957283
CTAACGC6000.024.2957363
TCTAACG6550.023.6922452
GAGTACT122000.023.0941112-13
AGAGTAC164600.022.98446810-11
CAGAGTA174200.022.29785510-11
Produced by FastQC (version 0.11.5)