##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146610_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3112102 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.330028385959075 33.0 31.0 34.0 30.0 34.0 2 32.38776556809513 34.0 31.0 34.0 30.0 34.0 3 32.49648661901185 34.0 31.0 34.0 30.0 34.0 4 36.01748657338352 37.0 35.0 37.0 35.0 37.0 5 36.057139836676306 37.0 35.0 37.0 35.0 37.0 6 36.12504538732985 37.0 36.0 37.0 35.0 37.0 7 36.086025136708244 37.0 36.0 37.0 35.0 37.0 8 36.08874676986808 37.0 36.0 37.0 35.0 37.0 9 37.85970222055704 39.0 38.0 39.0 35.0 39.0 10-11 37.808340311467944 39.0 38.0 39.0 35.0 39.0 12-13 37.78364623010428 39.0 38.0 39.0 35.0 39.0 14-15 39.123098793034416 40.0 39.0 41.0 36.0 41.0 16-17 39.064502705888174 40.0 38.5 41.0 36.0 41.0 18-19 39.17382206624333 40.0 39.0 41.0 36.0 41.0 20-21 39.23766444673086 40.0 39.0 41.0 36.0 41.0 22-23 39.205715461768285 40.0 39.0 41.0 36.0 41.0 24-25 39.19700944891909 41.0 39.0 41.0 36.0 41.0 26-27 39.11681670459387 40.0 39.0 41.0 35.0 41.0 28-29 39.05779775213023 40.0 39.0 41.0 35.0 41.0 30-31 38.914912814554285 40.0 38.5 41.0 35.0 41.0 32-33 38.80284402632047 40.0 38.0 41.0 35.0 41.0 34-35 38.72980496783203 40.0 38.0 41.0 35.0 41.0 36-37 38.66218009563954 40.0 38.0 41.0 35.0 41.0 38-39 38.576779295794296 40.0 38.0 41.0 35.0 41.0 40-41 38.47304506728892 40.0 38.0 41.0 34.5 41.0 42-43 38.358303969471436 40.0 38.0 41.0 34.0 41.0 44-45 38.202228429530905 40.0 38.0 41.0 34.0 41.0 46-47 37.949459882741635 40.0 38.0 41.0 33.0 41.0 48-49 37.65080337983781 40.0 37.0 41.0 33.0 41.0 50-51 37.53645237206236 40.0 37.0 41.0 33.0 41.0 52-53 37.42923673452863 40.0 37.0 41.0 32.0 41.0 54-55 37.26866519799158 40.0 36.0 41.0 32.0 41.0 56-57 37.11230978290557 39.5 36.0 41.0 32.0 41.0 58-59 36.92279526827848 39.0 35.0 41.0 32.0 41.0 60-61 36.72130524642187 39.0 35.0 41.0 31.0 41.0 62-63 36.47544794482957 38.5 35.0 41.0 31.0 41.0 64-65 36.20152070851148 38.0 35.0 40.0 31.0 41.0 66-67 35.93410113164671 37.0 35.0 40.0 31.0 41.0 68-69 35.64639317734445 37.0 35.0 40.0 31.0 41.0 70-71 35.34984296787188 36.0 35.0 39.0 30.0 41.0 72-73 35.03775599257351 36.0 35.0 39.0 30.0 41.0 74-75 34.728898988529295 35.0 34.5 38.5 30.0 40.0 76-77 34.44020343806212 35.0 34.0 37.0 30.0 39.5 78-79 34.16675770909822 35.0 34.0 37.0 29.5 39.0 80-81 33.9193503297771 35.0 34.0 36.5 29.0 39.0 82-83 33.67422629463944 35.0 34.0 36.0 29.0 37.5 84-85 33.47390766755074 35.0 34.0 36.0 29.0 37.0 86-87 33.29438125742665 35.0 34.0 35.0 29.0 37.0 88-89 33.10263545346521 35.0 34.0 35.0 29.0 36.0 90-91 33.02393269886398 35.0 34.0 35.0 29.0 36.0 92-93 32.931079058462736 35.0 34.0 35.0 29.0 36.0 94-95 32.86260379640513 35.0 34.0 35.0 29.0 36.0 96-97 32.80646151700684 35.0 34.0 35.0 29.0 35.0 98-99 32.741382030537565 35.0 34.0 35.0 29.0 35.0 100 32.0099659329932 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3655969061027662 1101 2 0.328091125245237 1101 3 0.3582479528058613 1101 4 0.3069061192184108 1101 5 0.24251263289649216 1101 6 0.2792002485213416 1101 7 0.2911047018588775 1101 8 0.2592458079855433 1101 9 0.28532212996788076 1101 10-11 0.3011312587698711 1101 12-13 0.27330947506541037 1101 14-15 0.39457922620492525 1101 16-17 0.4089363328917699 1101 18-19 0.39188688310421327 1101 20-21 0.3407121688290573 1101 22-23 0.35137525913036427 1101 24-25 0.3377629522289851 1101 26-27 0.3244416827906704 1101 28-29 0.37129627234931206 1101 30-31 0.4757444476299284 1101 32-33 0.43599013922065666 1101 34-35 0.4738561037284015 1101 36-37 0.4747050371240249 1101 38-39 0.5267080964420501 1101 40-41 0.5596000142707993 1101 42-43 0.5522217649454788 1101 44-45 0.4893720300543549 1101 46-47 0.41080365383560746 1101 48-49 0.4207014789757437 1101 50-51 0.39986055006378507 1101 52-53 0.4045585844183961 1101 54-55 0.4155014910851449 1101 56-57 0.39662614888868575 1101 58-59 0.3252065547959049 1101 60-61 0.3613727765827335 1101 62-63 0.35644007449609205 1101 64-65 0.2781965635661692 1101 66-67 0.27960812455500417 1101 68-69 0.2776634955309518 1101 70-71 0.21168887246021484 1101 72-73 0.3146480280410131 1101 74-75 0.3251494630653937 1101 76-77 0.24014032661875007 1101 78-79 0.22858151503515955 1101 80-81 0.24289127005022237 1101 82-83 0.2975235695185816 1101 84-85 0.28230099005298825 1101 86-87 0.23351371739178006 1101 88-89 0.26191189179458974 1101 90-91 0.27197759586489667 1101 92-93 0.30574728388180006 1101 94-95 0.2927271462551033 1101 96-97 0.2682607542617248 1101 98-99 0.20434783593479722 1101 100 0.2728980229241955 1102 1 0.2814743636225856 1102 2 0.25860133264703933 1102 3 0.27249555971913964 1102 4 0.2206302696291047 1102 5 0.20397013673393616 1102 6 0.16611726097184487 1102 7 0.21464865650710863 1102 8 0.22202274187761617 1102 9 0.33386001948488087 1102 10-11 0.29564055968428704 1102 12-13 0.27286753392657914 1102 14-15 0.39426689335280685 1102 16-17 0.4626795035539999 1102 18-19 0.36393424878225034 1102 20-21 0.384823283174903 1102 22-23 0.38064301369605147 1102 24-25 0.33110677752580386 1102 26-27 0.35323193314216184 1102 28-29 0.3840728922816439 1102 30-31 0.4350260677991571 1102 32-33 0.38150122856389146 1102 34-35 0.3824773809508528 1102 36-37 0.401110563500346 1102 38-39 0.47921155428039697 1102 40-41 0.46596994332160335 1102 42-43 0.4291265729248579 1102 44-45 0.43642634565924254 1102 46-47 0.4816855153542434 1102 48-49 0.5583635178759181 1102 50-51 0.5631735559314137 1102 52-53 0.4333111310599733 1102 54-55 0.38444251060944623 1102 56-57 0.3807789171287439 1102 58-59 0.3797198401447659 1102 60-61 0.3528915254018159 1102 62-63 0.3009349332627309 1102 64-65 0.2691272087191692 1102 66-67 0.31328150482246286 1102 68-69 0.3134595004789631 1102 70-71 0.34517770148744376 1102 72-73 0.30742358621860433 1102 74-75 0.29375896965198933 1102 76-77 0.2622214572391215 1102 78-79 0.24560341287038057 1102 80-81 0.25927052085096847 1102 82-83 0.2491021876048265 1102 84-85 0.18493151595837531 1102 86-87 0.25459742647783656 1102 88-89 0.2594765346768071 1102 90-91 0.23916218192611183 1102 92-93 0.2606347806922571 1102 94-95 0.20859084663020155 1102 96-97 0.1885913595703812 1102 98-99 0.2722339222097929 1102 100 0.25932565777418404 1103 1 0.22932581166106303 1103 2 0.22256730682316572 1103 3 0.21682787921723445 1103 4 0.139502565252684 1103 5 0.11532655244730705 1103 6 0.13475899262068225 1103 7 0.15106608340299488 1103 8 0.09850816433423404 1103 9 0.1801908361161395 1103 10-11 0.21981705225010728 1103 12-13 0.2234962273333494 1103 14-15 0.3285981706041383 1103 16-17 0.43276749060011355 1103 18-19 0.3510898546787118 1103 20-21 0.2821294617108876 1103 22-23 0.3393940277889058 1103 24-25 0.34924487985941255 1103 26-27 0.3311303949007609 1103 28-29 0.3679058121757066 1103 30-31 0.41641845758708484 1103 32-33 0.36919661671028337 1103 34-35 0.39620898209361144 1103 36-37 0.41575705636205385 1103 38-39 0.41041087447935354 1103 40-41 0.44377718088630047 1103 42-43 0.45878445542389557 1103 44-45 0.47459463119422196 1103 46-47 0.5874038949785003 1103 48-49 0.6151415113654082 1103 50-51 0.5852588918727193 1103 52-53 0.5847878147632528 1103 54-55 0.6058593346804031 1103 56-57 0.5355592139394219 1103 58-59 0.5794068680147078 1103 60-61 0.5772038964734634 1103 62-63 0.577753079750515 1103 64-65 0.510464719356932 1103 66-67 0.5493137144430236 1103 68-69 0.5746989284091413 1103 70-71 0.5773128853093112 1103 72-73 0.600761178337109 1103 74-75 0.465603352273142 1103 76-77 0.4840943470758461 1103 78-79 0.49203068149049045 1103 80-81 0.5278941953537597 1103 82-83 0.472935114123338 1103 84-85 0.49736113117941727 1103 86-87 0.49869504172007595 1103 88-89 0.492376814846871 1103 90-91 0.4672109082873135 1103 92-93 0.40019860594104273 1103 94-95 0.4748944180035721 1103 96-97 0.39619973888107296 1103 98-99 0.4057505356674085 1103 100 0.38507195724612586 1104 1 0.2381496999880568 1104 2 0.24471007089139363 1104 3 0.16675352293169965 1104 4 0.12716629903442822 1104 5 0.0771236063251095 1104 6 0.14303012107814794 1104 7 0.11320394554085311 1104 8 0.12890566538391113 1104 9 0.18504462621837092 1104 10-11 0.18166188443655784 1104 12-13 0.1695310930887146 1104 14-15 0.3202575464250046 1104 16-17 0.3282886102671938 1104 18-19 0.26362320642014225 1104 20-21 0.27373190688476967 1104 22-23 0.2612142107115787 1104 24-25 0.2659809129774402 1104 26-27 0.19994475859090244 1104 28-29 0.17797079894877044 1104 30-31 0.2887466211419607 1104 32-33 0.24793322689099284 1104 34-35 0.23661846908695594 1104 36-37 0.22196528912309077 1104 38-39 0.2504583270268128 1104 40-41 0.23569900130191002 1104 42-43 0.23194835156915872 1104 44-45 0.26309218436568926 1104 46-47 0.3250233148960575 1104 48-49 0.4063579036687628 1104 50-51 0.24723027969814382 1104 52-53 0.1692378109368491 1104 54-55 0.30182641108225994 1104 56-57 0.22250898776808015 1104 58-59 0.16305132914364862 1104 60-61 0.1838249152062872 1104 62-63 0.19622296390362948 1104 64-65 0.20762359050648627 1104 66-67 0.1572899256024627 1104 68-69 0.1418803494725509 1104 70-71 0.18268732453467607 1104 72-73 0.1530181346313313 1104 74-75 0.1502114201886613 1104 76-77 0.130134150957268 1104 78-79 0.14942758065758 1104 80-81 0.17102381418214208 1104 82-83 0.16727405675420925 1104 84-85 0.19659965145831393 1104 86-87 0.20698002795537462 1104 88-89 0.23288147064656073 1104 90-91 0.162241705647304 1104 92-93 0.19739289715707287 1104 94-95 0.2513790875783002 1104 96-97 0.2165672595591559 1104 98-99 0.20079963770408682 1104 100 0.26963615081653103 1105 1 0.17406008854539579 1105 2 0.1808666348576935 1105 3 0.1820598233899844 1105 4 0.1477235928419347 1105 5 0.09386443258501487 1105 6 0.11395761388492787 1105 7 0.1510093905994907 1105 8 0.14664871306242588 1105 9 0.13650015329804432 1105 10-11 0.16402380571283715 1105 12-13 0.2218033864507376 1105 14-15 0.31240531456190723 1105 16-17 0.3508739312348581 1105 18-19 0.29818599425183123 1105 20-21 0.24578163910911144 1105 22-23 0.25446594977462667 1105 24-25 0.27848314974457367 1105 26-27 0.2886415787503296 1105 28-29 0.27386023863766695 1105 30-31 0.3759899731705403 1105 32-33 0.3269565064894948 1105 34-35 0.2156907891030002 1105 36-37 0.202478573835279 1105 38-39 0.242146088560105 1105 40-41 0.2809728152912214 1105 42-43 0.3174968517933934 1105 44-45 0.27718392962805183 1105 46-47 0.36846511365364165 1105 48-49 0.4158921095471726 1105 50-51 0.39456109587294463 1105 52-53 0.42125786434971957 1105 54-55 0.42175381629012776 1105 56-57 0.3586159123499044 1105 58-59 0.28214931489272743 1105 60-61 0.28112095386360636 1105 62-63 0.31836445037483685 1105 64-65 0.33081319423745015 1105 66-67 0.2034451194319047 1105 68-69 0.2492802115604107 1105 70-71 0.24717210441058057 1105 72-73 0.2299089422109546 1105 74-75 0.3243166571811642 1105 76-77 0.18769884125767078 1105 78-79 0.2280303105991166 1105 80-81 0.267511546965018 1105 82-83 0.27072335757262067 1105 84-85 0.287358864505606 1105 86-87 0.23369844536494355 1105 88-89 0.29887407490237194 1105 90-91 0.2416570839735357 1105 92-93 0.22788428214082046 1105 94-95 0.2822553088157207 1105 96-97 0.2158133099337718 1105 98-99 0.19055685394537392 1105 100 0.2005018901326423 1106 1 0.10059585077718936 1106 2 0.05888987412760116 1106 3 0.12375620078510252 1106 4 0.11482307623219157 1106 5 0.0636965703109098 1106 6 0.054894032282476246 1106 7 0.12161411122656318 1106 8 0.07478073077707847 1106 9 0.08390184204961315 1106 10-11 0.08713886135652871 1106 12-13 0.13769276719331458 1106 14-15 0.1898249579698046 1106 16-17 0.2894368329782111 1106 18-19 0.23537239544621968 1106 20-21 0.1685067398948945 1106 22-23 0.1504882023008136 1106 24-25 0.09964156963580706 1106 26-27 0.14062093752929883 1106 28-29 0.09284363419446606 1106 30-31 0.06391992228779486 1106 32-33 0.06392678150346853 1106 34-35 0.06301541758647033 1106 36-37 0.05426963842838006 1106 38-39 0.041680726917945776 1106 40-41 0.13956638173409885 1106 42-43 0.10459110344195466 1106 44-45 0.0982552198315858 1106 46-47 0.17842499241268683 1106 48-49 0.19267659238346369 1106 50-51 0.07129792471073415 1106 52-53 0.10418397035467564 1106 54-55 0.11971080828419645 1106 56-57 0.044746979954922494 1106 58-59 0.024477001331199233 1106 60-61 -0.024969030113439317 1106 62-63 0.05979829017918803 1106 64-65 0.020646902277945856 1106 66-67 -0.025919129890887405 1106 68-69 0.011665820429108464 1106 70-71 0.018121744738245127 1106 72-73 -0.02569993242298807 1106 74-75 -0.005882730198123909 1106 76-77 -0.07118697597969259 1106 78-79 -0.16715284838078048 1106 80-81 -0.07259253043702785 1106 82-83 -0.07721580748933121 1106 84-85 -0.00497772302254873 1106 86-87 -0.0351079531620897 1106 88-89 -0.047177643300024386 1106 90-91 -0.04711372108596379 1106 92-93 -0.0425585208331114 1106 94-95 -0.015866120661655714 1106 96-97 -0.0038660290528937935 1106 98-99 -0.06179413770433939 1106 100 -0.0462953265094832 1107 1 0.004724850988907292 1107 2 0.06548579095329643 1107 3 0.06425264937307418 1107 4 -0.03846041688430546 1107 5 0.05001500218860144 1107 6 0.07503231745412364 1107 7 0.07865428581273903 1107 8 0.0744721473553227 1107 9 0.12018830581370565 1107 10-11 0.11393197212520789 1107 12-13 0.11055007842956854 1107 14-15 0.159304785147782 1107 16-17 0.23502749111379728 1107 18-19 0.16037024048061 1107 20-21 0.14114401024755097 1107 22-23 0.18542093628105505 1107 24-25 0.17257170483753725 1107 26-27 0.2099815312493476 1107 28-29 0.18703584162835085 1107 30-31 0.24325168778199924 1107 32-33 0.18510023466150471 1107 34-35 0.14703960754183498 1107 36-37 0.12351439975721235 1107 38-39 0.13206534211857957 1107 40-41 0.12430114110456714 1107 42-43 0.1198033907544982 1107 44-45 0.15632168901635168 1107 46-47 0.20214107059914 1107 48-49 0.2293765895038007 1107 50-51 0.13818021230933653 1107 52-53 0.16773861793863176 1107 54-55 0.19753183047359357 1107 56-57 0.17075604678736056 1107 58-59 0.136030912830563 1107 60-61 0.13333156257146328 1107 62-63 0.19619134940089822 1107 64-65 0.21212116950114535 1107 66-67 0.1543314737147412 1107 68-69 0.1094072633979053 1107 70-71 0.10155452698396772 1107 72-73 0.09808710416493227 1107 74-75 0.1338983367762907 1107 76-77 0.13844377341354885 1107 78-79 0.03696647481555715 1107 80-81 0.06321170416367039 1107 82-83 0.07217097683606966 1107 84-85 0.06416816341358356 1107 86-87 0.08904961313981374 1107 88-89 0.1566938468924235 1107 90-91 0.10129892789726114 1107 92-93 0.1021650231491833 1107 94-95 0.12709082987018405 1107 96-97 0.1431633759670845 1107 98-99 0.1396455419620537 1107 100 0.010779930247139191 1108 1 0.042759954425172 1108 2 0.006348317216058774 1108 3 0.0320270079435474 1108 4 0.04203634626307462 1108 5 0.01826805889702854 1108 6 0.06624333213283506 1108 7 0.011177947939636113 1108 8 0.04678518230151951 1108 9 0.0502570846589947 1108 10-11 0.06513108293076186 1108 12-13 -0.02089344325732867 1108 14-15 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2214 78-79 -0.30191231562022125 2214 80-81 -0.29938335596529697 2214 82-83 -0.3353241426532989 2214 84-85 -0.2523766157429037 2214 86-87 -0.21782367093400268 2214 88-89 -0.1262531833711762 2214 90-91 -0.2397121830578044 2214 92-93 -0.26249053151781254 2214 94-95 -0.24172523460536866 2214 96-97 -0.21418564061665535 2214 98-99 -0.1923570859441952 2214 100 -0.4331207285207235 2215 1 -0.35640915464349376 2215 2 -0.34502491568407834 2215 3 -0.3789911947828202 2215 4 -0.26796645860357415 2215 5 -0.27799880361346396 2215 6 -0.23217483147897156 2215 7 -0.26080875130142545 2215 8 -0.2405652756234531 2215 9 -0.38537732333121255 2215 10-11 -0.3133414074701335 2215 12-13 -0.3657679446708286 2215 14-15 -0.44053004971839016 2215 16-17 -0.3445165960833805 2215 18-19 -0.40686691128792773 2215 20-21 -0.44919661154214197 2215 22-23 -0.4280616415098919 2215 24-25 -0.4692048497352346 2215 26-27 -0.3839073935752495 2215 28-29 -0.4300423352212235 2215 30-31 -0.4072506354033223 2215 32-33 -0.43562587986118473 2215 34-35 -0.46202258881595526 2215 36-37 -0.5013884289391441 2215 38-39 -0.4439558868407829 2215 40-41 -0.44723374701599994 2215 42-43 -0.4013388592239977 2215 44-45 -0.44837153714976097 2215 46-47 -0.46328575630830926 2215 48-49 -0.41291175905120525 2215 50-51 -0.40280921123328284 2215 52-53 -0.363913840214245 2215 54-55 -0.2967960663672784 2215 56-57 -0.38222048441560474 2215 58-59 -0.4818805086722975 2215 60-61 -0.374188099475262 2215 62-63 -0.4015748640353962 2215 64-65 -0.329363818995958 2215 66-67 -0.4016602369615683 2215 68-69 -0.433568419258755 2215 70-71 -0.3605677916618646 2215 72-73 -0.2556197448789135 2215 74-75 -0.32566159227040004 2215 76-77 -0.2692937981071992 2215 78-79 -0.3031127437057535 2215 80-81 -0.351096305162919 2215 82-83 -0.2919250002429763 2215 84-85 -0.31832654650298053 2215 86-87 -0.24607714368141131 2215 88-89 -0.1978737202302625 2215 90-91 -0.2664438855994504 2215 92-93 -0.21217484144044363 2215 94-95 -0.2656607415054282 2215 96-97 -0.2551342917593402 2215 98-99 -0.16734002278144544 2215 100 -0.24378419400322926 2216 1 -0.4992626568906182 2216 2 -0.48255018145189155 2216 3 -0.46399656917311916 2216 4 -0.3438440789063506 2216 5 -0.2878635468464097 2216 6 -0.3660053380823385 2216 7 -0.33033517628152964 2216 8 -0.36053620331528435 2216 9 -0.4492468843485611 2216 10-11 -0.47519401577444853 2216 12-13 -0.47343932332078253 2216 14-15 -0.5252576424975359 2216 16-17 -0.504937546545456 2216 18-19 -0.5527262847306176 2216 20-21 -0.5807063015448577 2216 22-23 -0.580201093521417 2216 24-25 -0.5679498337653968 2216 26-27 -0.5831802025336899 2216 28-29 -0.5359642539935621 2216 30-31 -0.5563798615064499 2216 32-33 -0.6107693630487745 2216 34-35 -0.6404965686401383 2216 36-37 -0.5754291731043679 2216 38-39 -0.5863921186648327 2216 40-41 -0.6011258942727551 2216 42-43 -0.6075700239410082 2216 44-45 -0.5800178486006615 2216 46-47 -0.5748913266606834 2216 48-49 -0.5450265394678695 2216 50-51 -0.5833930187549754 2216 52-53 -0.5606772612398387 2216 54-55 -0.49804067089342396 2216 56-57 -0.5309016657694627 2216 58-59 -0.6075384766467593 2216 60-61 -0.6273976148294622 2216 62-63 -0.6398196387633419 2216 64-65 -0.5652983572353918 2216 66-67 -0.581497257233984 2216 68-69 -0.5442898602393669 2216 70-71 -0.5078582709409432 2216 72-73 -0.48958041752871395 2216 74-75 -0.446199123301561 2216 76-77 -0.4499857527752553 2216 78-79 -0.4036162803765819 2216 80-81 -0.4672913134966805 2216 82-83 -0.4065338197631334 2216 84-85 -0.4606886868750024 2216 86-87 -0.3592247270457989 2216 88-89 -0.3218272628044758 2216 90-91 -0.39356166371456425 2216 92-93 -0.4552892431197719 2216 94-95 -0.3850946135044637 2216 96-97 -0.447820183563131 2216 98-99 -0.3471541041440389 2216 100 -0.5041695247543991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 22.0 8 111.0 9 211.0 10 217.0 11 159.0 12 168.0 13 244.0 14 392.0 15 758.0 16 1346.0 17 2511.0 18 4288.0 19 6588.0 20 9441.0 21 14105.0 22 20105.0 23 23888.0 24 22942.0 25 20103.0 26 18480.0 27 20234.0 28 24880.0 29 30892.0 30 38188.0 31 48728.0 32 62104.0 33 81767.0 34 118467.0 35 183681.0 36 333532.0 37 747447.0 38 955519.0 39 319911.0 40 673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29885810942818 19.845972020853257 13.645650173262998 24.209519696455565 2 15.23717376697501 20.79183136509275 39.995777636318245 23.975217231614 3 17.50200990072922 26.45421378660156 31.041286027992726 25.002490284676494 4 12.250808148516077 17.974894751816826 39.082078312042576 30.69221878762452 5 12.707336063163774 38.33894005758929 35.371237552766885 13.582486326480051 6 29.383445213160797 39.027143577862255 17.936066221718075 13.653344987258876 7 25.824997324958282 33.56770876009487 23.187839983342442 17.419453931604405 8 25.69778692061827 35.006871590111984 20.56624987267395 18.7290916165958 9 26.479331152383008 14.773469610060605 20.802326661201974 37.94487257635441 10-11 24.4707668173582 26.155306213141817 28.80053224562088 20.573394723879108 12-13 25.4227367869048 24.17004648305229 28.682543181425284 21.724673548617623 14-15 22.735123313799903 25.07622663917399 26.75547805164421 25.433171995381898 16-17 21.57220930486631 28.02428398646313 27.456659378964375 22.94684732970619 18-19 21.45039753722448 26.954561367514557 30.042108243039223 21.55293285222174 20-21 22.570674692132485 26.069348494831534 29.798317434871414 21.56165937816457 22-23 22.54090643558598 25.944329588169023 29.516513276235806 21.99825070000919 24-25 22.107501617877563 26.11286519529244 29.906619384583156 21.87301380224684 26-27 22.00882525182012 26.340926563076128 29.535706281223966 22.114541903879786 28-29 21.85627270571466 26.458033830510697 29.73223564009149 21.953457823683156 30-31 22.220519190198633 26.215848383878843 29.546898690320173 22.01673373560235 32-33 22.31809021070071 26.43125588832207 29.151853479909978 22.098800421067246 34-35 22.411967359002293 26.33305147635912 29.502231372175398 21.752749792463188 36-37 22.397034542115378 26.257065366965293 29.323676829741462 22.022223261177864 38-39 22.305992723823405 26.239253950902196 29.48495119331995 21.969802131954445 40-41 22.26734385533543 26.043658232655826 29.490466510843866 22.198531401164878 42-43 22.39974782317546 26.087207295904825 29.095142125804358 22.417902755115353 44-45 22.3720110472561 26.496978713627676 28.89872866583014 22.232281573286077 46-47 22.57511161266565 26.400837761744313 28.412580950110243 22.61146967547979 48-49 22.899173772539864 26.51628467878733 28.119962199121684 22.464579349551123 50-51 22.65314568738428 26.73190017550839 27.78487016171064 22.830083975396693 52-53 22.403738586231 26.875916487512956 27.82124719902458 22.899097727231464 54-55 22.48354399060437 26.859886539088745 27.715406230870943 22.94116323943594 56-57 22.726116679977643 26.71910403720197 27.614642683454083 22.940136599366312 58-59 22.380994765861555 26.69643571527495 28.052832376145904 22.869737142717593 60-61 22.448824829563375 26.888250480667192 27.81902653225069 22.84389815751874 62-63 22.489402226958227 26.608608195289822 27.781854170465692 23.120135407286256 64-65 22.229187901097784 26.58731255326013 27.965424455123962 23.218075090518123 66-67 22.521245594152035 26.58563130431094 27.71816197453967 23.174961126997353 68-69 22.21772887473297 26.451795753738423 27.999044346929697 23.33143102459891 70-71 22.395877098962767 26.136385363225738 27.99993958836864 23.467797949442858 72-73 22.44557898217441 26.29496845584509 27.936283152853786 23.32316940912671 74-75 22.40532699057616 26.58456729287683 27.735498799646912 23.27460691690009 76-77 22.39529876765372 26.526631211941066 27.563039251916 23.51503076848921 78-79 22.270459404129685 26.49668884173888 27.657433854757613 23.57541789937382 80-81 22.150974298531246 26.556497393446364 27.764877246080932 23.527651061941455 82-83 22.34609271333786 26.284170713669795 27.989623648390392 23.38011292460195 84-85 22.386305103437284 26.422433363677467 27.580389186778355 23.610872346106895 86-87 22.010866290832737 26.63602070819348 27.65337184535916 23.699741155614625 88-89 22.204847398960574 26.241845543622926 27.540308768799992 24.012998288616505 90-91 22.015496926352967 26.215955073437524 27.772443760662007 23.996104239547495 92-93 22.244383049634042 26.20047633461414 27.550465955763602 24.004674659988215 94-95 21.679221168359923 26.136568664702825 27.869949447277918 24.31426071965933 96-97 21.78783946423169 26.06576638251157 27.753345298491382 24.393048854765357 98-99 21.693857599255807 26.309892283259117 27.582586985144264 24.413663132340808 100 22.052056597014772 25.94842316950743 27.338106131300393 24.661414102177407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 200.0 1 132.5 2 217.0 3 366.0 4 244.0 5 204.0 6 444.5 7 1187.0 8 2233.0 9 2751.5 10 2086.0 11 1373.0 12 1495.5 13 1702.5 14 2009.0 15 2395.0 16 2772.0 17 3303.0 18 3845.0 19 4348.5 20 5019.5 21 5879.5 22 7176.5 23 8945.5 24 10897.0 25 13334.5 26 16727.0 27 20453.5 28 24109.5 29 29188.0 30 35664.0 31 41970.0 32 49952.5 33 56802.5 34 60881.0 35 67011.0 36 75777.0 37 84426.0 38 88966.0 39 90214.0 40 91165.5 41 91102.5 42 91710.5 43 95724.0 44 101388.5 45 111212.0 46 118975.0 47 121511.5 48 123731.5 49 122426.0 50 119299.0 51 119096.0 52 117840.5 53 115576.5 54 112241.5 55 101875.5 56 91531.0 57 81479.0 58 71586.0 59 64418.5 60 54080.0 61 41995.0 62 32333.0 63 24946.5 64 18444.0 65 12976.5 66 9626.5 67 7270.5 68 5221.5 69 3830.0 70 3030.0 71 2341.5 72 1617.0 73 1220.5 74 951.0 75 664.5 76 443.5 77 265.0 78 150.5 79 97.0 80 49.0 81 22.5 82 11.5 83 7.0 84 4.0 85 1.5 86 2.5 87 3.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0022492836031723896 2 0.0032453949131487337 3 2.5706098321970166E-4 4 0.05131579877523294 5 0.002088620488660076 6 0.004241506223125078 7 3.213262290246271E-5 8 4.1772409773201526E-4 9 9.639786870738812E-5 10-11 1.6066311451231355E-4 12-13 0.0 14-15 3.213262290246271E-5 16-17 3.213262290246271E-4 18-19 1.1246418015861948E-4 20-21 2.73127294670933E-4 22-23 0.0 24-25 0.0 26-27 3.0525991757339574E-4 28-29 0.0 30-31 1.767294259635449E-4 32-33 3.213262290246271E-4 34-35 1.1246418015861948E-4 36-37 8.033155725615677E-5 38-39 8.997134412689558E-4 40-41 6.426524580492542E-4 42-43 0.0 44-45 5.46254589341866E-4 46-47 0.0 48-49 1.6066311451231355E-4 50-51 0.0 52-53 5.301882778906348E-4 54-55 5.46254589341866E-4 56-57 2.73127294670933E-4 58-59 0.0010603765557812696 60-61 6.908513924029482E-4 62-63 3.0525991757339574E-4 64-65 5.783872122443288E-4 66-67 0.0014459680306108218 68-69 0.003020466552831495 70-71 0.003807715813941831 72-73 0.0036952516337832118 74-75 0.0032453949131487337 76-77 0.004884158681174332 78-79 0.0015744985222206729 80-81 0.001365636473354665 82-83 8.19381884012799E-4 84-85 7.390503267566422E-4 86-87 1.6066311451231353E-5 88-89 0.0 90-91 3.213262290246271E-5 92-93 1.2853049160985083E-4 94-95 1.445968030610822E-4 96-97 3.3739254047585846E-4 98-99 1.445968030610822E-4 100 0.07249119726795587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3112102.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.491393165571665 #Duplication Level Percentage of deduplicated Percentage of total 1 68.17865429772918 36.469712025394244 2 16.44652230338221 17.594947814731224 3 6.702422878990911 10.755658121460765 4 3.1991276554442853 6.84503180816895 5 1.7524722444200471 4.687109091901228 6 1.055077590014239 3.38625421125813 7 0.6806854186538649 2.5487567945899943 8 0.4537142511004854 1.9415845912351177 9 0.31772022445534537 1.5295767698695104 >10 1.1624019825943817 9.74314642881932 >50 0.03311525170406133 1.216936942650579 >100 0.01658750692669154 1.5281779754939266 >500 0.0010301462767172945 0.40882128502539217 >1k 1.8729932303950694E-4 0.14046500223149058 >5k 2.809489845592604E-4 1.2038211371700964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9789 0.31454624559220745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9160 0.2943348257865584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5082 0.1632979895903155 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.213262290246271E-5 2 0.0 0.0 0.0 0.0 3.213262290246271E-5 3 0.0 0.0 0.0 0.0 3.213262290246271E-5 4 0.0 0.0 0.0 0.0 3.213262290246271E-5 5 0.0 0.0 0.0 0.0 3.213262290246271E-5 6 0.0 0.0 0.0 0.0 9.639786870738812E-5 7 0.0 0.0 0.0 0.0 9.639786870738812E-5 8 0.0 0.0 0.0 0.0 9.639786870738812E-5 9 0.0 0.0 0.0 0.0 9.639786870738812E-5 10-11 0.0 0.0 0.0 0.0 9.639786870738812E-5 12-13 0.0 0.0 0.0 0.0 4.0165778628078384E-4 14-15 0.0 0.0 0.0 0.0 4.6592303208570923E-4 16-17 0.0 0.0 0.0 0.0 5.301882778906347E-4 18-19 0.0 0.0 0.0 0.0 5.944535236955601E-4 20-21 0.0 0.0 0.0 0.0 6.265861465980228E-4 22-23 0.0 0.0 0.0 4.819893435369406E-5 6.747850809517169E-4 24-25 0.0 0.0 0.0 2.2492836031723896E-4 6.747850809517169E-4 26-27 0.0 0.0 0.0 7.069177038541796E-4 7.390503267566422E-4 28-29 0.0 0.0 0.0 0.0028758697497704125 7.71182949659105E-4 30-31 0.0 0.0 0.0 0.009784383673799895 7.872492611103363E-4 32-33 0.0 0.0 0.0 0.0278750503678864 8.033155725615677E-4 34-35 0.0 0.0 0.0 0.05676227835720038 8.354481954640304E-4 36-37 0.0 0.0 0.0 0.10287259222223435 8.354481954640304E-4 38-39 0.0 0.0 0.0 0.17076882441513808 9.157797527201872E-4 40-41 0.0 0.0 0.0 0.24634475348173035 9.639786870738813E-4 42-43 0.0 0.0 0.0 0.3275278252448024 9.800449985251126E-4 44-45 0.0 0.0 0.0 0.43284249680762393 0.0010282439328788066 46-47 0.0 0.0 0.0 0.5616782483350482 0.001092509178683732 48-49 0.0 0.0 0.0 0.7035598447608722 0.001140708113037426 50-51 0.0 0.0 0.0 0.8563022677277288 0.0012531722931960457 52-53 0.0 0.0 0.0 1.0106673881511596 0.0013013712275497399 54-55 0.0 0.0 0.0 1.1842156844473606 0.0013495701619034338 56-57 0.0 0.0 0.0 1.3891254206963652 0.0013656364733546653 58-59 0.0 0.0 0.0 1.6226331913285619 0.0013817027848058965 60-61 0.0 0.0 0.0 1.866535865469705 0.0013817027848058965 62-63 0.0 0.0 0.0 2.1186484247624273 0.0015102332764157472 64-65 0.0 0.0 0.0 2.3793403943701072 0.00154236589931821 66-67 0.0 0.0 0.0 2.680712264572305 0.0015905648336719041 68-69 0.0 0.0 0.0 3.02001669611086 0.001638763768025598 70-71 0.0 0.0 0.0 3.393060381697001 0.001638763768025598 72-73 0.0 0.0 0.0 3.772659122355244 0.001719095325281755 74-75 0.0 0.0 0.0 4.153125443831854 0.0018315595054403744 76-77 0.0 0.0 0.0 4.571492194021919 0.0018636921283428371 78-79 0.0 0.0 0.0 5.041817395445265 0.0018636921283428371 80-81 0.0 3.213262290246271E-5 0.0 5.55126406525236 0.0019600899970502253 82-83 0.0 3.213262290246271E-5 0.0 6.067747779475095 0.0021528857344650016 84-85 0.0 3.213262290246271E-5 0.0 6.575748481251578 0.0021528857344650016 86-87 0.0 3.213262290246271E-5 0.0 7.113503991835744 0.002281416226074852 88 0.0 3.213262290246271E-5 0.0 7.542104982420242 0.002409946717684703 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5750 0.0 57.90459 1 GTATCAA 10755 0.0 44.294174 1 ATCAACG 13605 0.0 34.356388 3 CAACGCA 13730 0.0 34.094013 5 TATCAAC 14240 0.0 33.3868 2 AACGCAG 14545 0.0 32.60369 6 TCAACGC 14475 0.0 32.518845 4 ACGCAGA 15855 0.0 29.55271 7 CGCAGAG 16110 0.0 29.055756 8 GCAGAGT 17665 0.0 26.20541 9 GTGGTAT 1715 0.0 25.77575 1 GTACATG 10345 0.0 25.63871 1 TACATGG 10330 0.0 25.219883 2 TAACGCC 585 0.0 24.918705 4 ACATGGG 10130 0.0 24.695728 3 CTAACGC 600 0.0 24.295736 3 TCTAACG 655 0.0 23.692245 2 GAGTACT 12200 0.0 23.09411 12-13 AGAGTAC 16460 0.0 22.984468 10-11 CAGAGTA 17420 0.0 22.297855 10-11 >>END_MODULE