FastQCFastQC Report
Mon 16 Jan 2017
ERR1146609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875933
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39700.038.8544731
GTATCAA59450.034.885461
CAACGCA59800.032.388515
ATCAACG62800.030.8428863
AACGCAG71300.028.0760236
TATCAAC72450.027.967672
TCAACGC73050.026.7078974
GTACATG117150.025.6115631
ACGCAGA75900.025.508017
TACATGG116750.025.4091362
CGCAGAG77600.024.9488778
ACATGGG121550.023.7482073
GTACTAG17300.022.2907891
CATGGGG94750.021.037714
CTGTGCG23100.020.5453599
TGATCGC10250.020.1712659
GCAGAGT94650.019.3619169
CTAGTAC26850.019.2604243
GTATTAG8100.019.1596091
TAGTACT26800.018.945274
Produced by FastQC (version 0.11.5)