##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146609_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3875933 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22503510767601 31.0 31.0 34.0 28.0 34.0 2 31.404191971326647 33.0 31.0 34.0 28.0 34.0 3 31.41205098230542 33.0 31.0 34.0 28.0 34.0 4 34.89992964274666 37.0 35.0 37.0 32.0 37.0 5 34.968340526010124 37.0 35.0 37.0 32.0 37.0 6 34.96631856123416 37.0 35.0 37.0 32.0 37.0 7 34.915245438969144 37.0 35.0 37.0 32.0 37.0 8 34.907739891272634 37.0 35.0 37.0 32.0 37.0 9 36.471024137930144 39.0 37.0 39.0 32.0 39.0 10-11 36.472969088990965 39.0 37.0 39.0 32.0 39.0 12-13 36.43277566975487 39.0 37.0 39.0 32.0 39.0 14-15 37.76403655584346 40.0 38.0 41.0 32.0 41.0 16-17 37.781471712746324 40.0 38.0 41.0 32.0 41.0 18-19 37.79902245472252 40.0 38.0 41.0 32.0 41.0 20-21 37.90571341145474 40.0 38.0 41.0 32.0 41.0 22-23 37.90836593408606 40.0 38.0 41.0 32.0 41.0 24-25 37.92792328969567 40.0 38.0 41.0 32.0 41.0 26-27 37.99113103864283 40.0 38.0 41.0 32.0 41.0 28-29 37.96950772884877 40.0 38.0 41.0 32.0 41.0 30-31 37.93215362597857 40.0 38.0 41.0 32.0 41.0 32-33 37.900449646575424 40.0 38.0 41.0 31.5 41.0 34-35 37.85126019980221 40.0 38.0 41.0 31.0 41.0 36-37 37.7758388754398 40.0 38.0 41.0 31.0 41.0 38-39 37.721732032003644 40.0 38.0 41.0 31.0 41.0 40-41 37.66863359093153 40.0 38.0 41.0 31.0 41.0 42-43 37.509331817655266 40.0 37.0 41.0 30.0 41.0 44-45 37.378100576041945 40.0 37.0 41.0 30.0 41.0 46-47 37.2662939478056 40.0 37.0 41.0 30.0 41.0 48-49 37.15324271601186 40.0 36.0 41.0 30.0 41.0 50-51 37.02228805296686 40.0 36.0 41.0 30.0 41.0 52-53 36.89732691457773 40.0 35.5 41.0 30.0 41.0 54-55 36.78150834908653 39.0 35.0 41.0 30.0 41.0 56-57 36.594057740420176 39.0 35.0 41.0 30.0 41.0 58-59 36.39848740935409 39.0 35.0 41.0 29.0 41.0 60-61 36.18168683514395 38.5 35.0 40.5 29.0 41.0 62-63 35.94297489146484 38.0 35.0 40.0 29.0 41.0 64-65 35.65271871314597 37.5 34.5 40.0 28.0 41.0 66-67 35.41604718141413 37.0 34.0 40.0 28.0 41.0 68-69 35.15995658335683 36.5 34.0 39.5 28.0 41.0 70-71 34.87724375008546 36.0 34.0 39.0 28.0 41.0 72-73 34.5396882505451 35.5 34.0 39.0 28.0 40.5 74-75 34.21187195444297 35.0 34.0 38.5 27.5 40.0 76-77 33.93602198490015 35.0 34.0 37.0 27.0 39.5 78-79 33.62073905818289 35.0 33.0 37.0 27.0 39.0 80-81 33.37454556103008 35.0 33.0 36.0 27.0 39.0 82-83 33.100132793833126 35.0 33.0 36.0 26.0 37.5 84-85 32.89011587661603 35.0 33.0 36.0 26.0 37.0 86-87 32.67397888456792 35.0 33.0 35.0 26.0 37.0 88-89 32.486756608021864 35.0 33.0 35.0 26.0 36.0 90-91 32.327912917999356 35.0 33.0 35.0 25.0 36.0 92-93 32.17064355859608 35.0 33.0 35.0 25.0 36.0 94-95 32.03908142891015 35.0 33.0 35.0 25.0 36.0 96-97 31.9383959939452 35.0 33.0 35.0 25.0 35.0 98-99 31.818204287845017 35.0 32.0 35.0 24.0 35.0 100 30.650320580876915 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26421646372075 1101 2 0.3020397162030868 1101 3 0.30965415634303284 1101 4 0.34928640938889544 1101 5 0.31486513366532165 1101 6 0.30795618794041246 1101 7 0.36773053928050814 1101 8 0.46147672683114394 1101 9 0.46530263227833046 1101 10-11 0.5128853131597708 1101 12-13 0.4865921162745437 1101 14-15 0.5799801934504885 1101 16-17 0.5374337303652439 1101 18-19 0.5403026904074366 1101 20-21 0.600396517534648 1101 22-23 0.5762735851575513 1101 24-25 0.6060385542577578 1101 26-27 0.591111334364129 1101 28-29 0.5510438985981168 1101 30-31 0.5971393083360752 1101 32-33 0.618075162605976 1101 34-35 0.5997416004041511 1101 36-37 0.5782539657692212 1101 38-39 0.5488471478861996 1101 40-41 0.592557074470534 1101 42-43 0.5857936521149298 1101 44-45 0.5419811246851509 1101 46-47 0.515283298520977 1101 48-49 0.4657776768883437 1101 50-51 0.5014895495742238 1101 52-53 0.41391088427239 1101 54-55 0.3835705287281783 1101 56-57 0.45920956339438845 1101 58-59 0.4399044758479178 1101 60-61 0.43539563363498246 1101 62-63 0.4811211637156987 1101 64-65 0.49590891351503785 1101 66-67 0.34373546728675564 1101 68-69 0.3556025872314592 1101 70-71 0.3909535353132867 1101 72-73 0.43385646153455326 1101 74-75 0.3731838525222244 1101 76-77 0.3534031555580768 1101 78-79 0.36922897654356746 1101 80-81 0.3571683160038859 1101 82-83 0.3834603887754824 1101 84-85 0.28123401745260423 1101 86-87 0.30754917061752707 1101 88-89 0.20665084653887078 1101 90-91 0.20969703980082244 1101 92-93 0.13321124756889446 1101 94-95 0.22201084339395294 1101 96-97 0.2567315373491539 1101 98-99 0.22091261944374807 1101 100 0.3491185475458778 1102 1 0.3074706160588683 1102 2 0.2777578664839453 1102 3 0.2792320397851107 1102 4 0.32307020543775167 1102 5 0.3416101493468844 1102 6 0.3151900046627176 1102 7 0.3284963645545602 1102 8 0.39927170167004533 1102 9 0.4384347104138939 1102 10-11 0.4909872131002757 1102 12-13 0.4332596569407272 1102 14-15 0.5691814671367226 1102 16-17 0.5777012624595059 1102 18-19 0.5285982400691012 1102 20-21 0.5160513616770004 1102 22-23 0.5518681989674121 1102 24-25 0.5013250076013165 1102 26-27 0.4178974448309489 1102 28-29 0.5120473424923873 1102 30-31 0.5215712296559403 1102 32-33 0.5862683030543394 1102 34-35 0.5535676383626225 1102 36-37 0.5170277780542918 1102 38-39 0.5536660156870497 1102 40-41 0.5460377145407307 1102 42-43 0.5467384792142909 1102 44-45 0.4985284315900742 1102 46-47 0.48013149993080617 1102 48-49 0.43832390221887607 1102 50-51 0.4996885012790244 1102 52-53 0.455190028794938 1102 54-55 0.36250756042411325 1102 56-57 0.4453693817705613 1102 58-59 0.4486591777972748 1102 60-61 0.4899067317481496 1102 62-63 0.3581390311232511 1102 64-65 0.4266216559238458 1102 66-67 0.4445134949387608 1102 68-69 0.34186136084204577 1102 70-71 0.3313733450167007 1102 72-73 0.31897258581728494 1102 74-75 0.4097036914710088 1102 76-77 0.3825703684744468 1102 78-79 0.3445881777416133 1102 80-81 0.3326475870882817 1102 82-83 0.35920910803002215 1102 84-85 0.3269554326259083 1102 86-87 0.22769702360408672 1102 88-89 0.24258955984348773 1102 90-91 0.3148952780194847 1102 92-93 0.2852729830294152 1102 94-95 0.38457034259222667 1102 96-97 0.35846193034640095 1102 98-99 0.41927437456924466 1102 100 0.4714328898780451 1103 1 0.30700834949691824 1103 2 0.32858063969821316 1103 3 0.2732807771803607 1103 4 0.30252829890982724 1103 5 0.29614516082405373 1103 6 0.32122200710401927 1103 7 0.40026423292951563 1103 8 0.36783319931451075 1103 9 0.44886174610804375 1103 10-11 0.42935168366283705 1103 12-13 0.4673191771520919 1103 14-15 0.5322406944382507 1103 16-17 0.5214254869778117 1103 18-19 0.510197835538456 1103 20-21 0.5240745091390124 1103 22-23 0.5345047944332677 1103 24-25 0.580960159815227 1103 26-27 0.5582868077695693 1103 28-29 0.534439409303836 1103 30-31 0.5701032014642493 1103 32-33 0.5997255989014363 1103 34-35 0.591672866219632 1103 36-37 0.6030929793743667 1103 38-39 0.6137435653154313 1103 40-41 0.5607009295003706 1103 42-43 0.582074520310357 1103 44-45 0.48466980697094897 1103 46-47 0.5421766363439389 1103 48-49 0.5230358542820213 1103 50-51 0.5872663528612634 1103 52-53 0.5687630444475289 1103 54-55 0.5122130387131918 1103 56-57 0.5968881148713621 1103 58-59 0.5872069503988868 1103 60-61 0.5774039893652656 1103 62-63 0.5330021161989649 1103 64-65 0.44688042600596134 1103 66-67 0.47232472184585106 1103 68-69 0.46824130457068236 1103 70-71 0.5211498145546614 1103 72-73 0.47331236297241475 1103 74-75 0.4616894975973693 1103 76-77 0.41461190332196196 1103 78-79 0.4327284366225683 1103 80-81 0.3552182005301532 1103 82-83 0.40788640425055434 1103 84-85 0.431649363732582 1103 86-87 0.37982487932907105 1103 88-89 0.3831440857088282 1103 90-91 0.4142280329239725 1103 92-93 0.34573442979392155 1103 94-95 0.40694030988201746 1103 96-97 0.41802050589437556 1103 98-99 0.3388853588046352 1103 100 0.4639719309075936 1104 1 0.23318916423007252 1104 2 0.2416227144048868 1104 3 0.14110775061750047 1104 4 0.24233342926476809 1104 5 0.17041474738449125 1104 6 0.2691157390545271 1104 7 0.358374912748495 1104 8 0.364264076829258 1104 9 0.30693952830154814 1104 10-11 0.3799805756806336 1104 12-13 0.3932653350741191 1104 14-15 0.503143042258877 1104 16-17 0.5193255645578034 1104 18-19 0.5241130155782159 1104 20-21 0.43347533777996006 1104 22-23 0.43128482688939584 1104 24-25 0.47785418178245465 1104 26-27 0.4331182454989104 1104 28-29 0.41171706297931365 1104 30-31 0.43950367649377853 1104 32-33 0.425476204867536 1104 34-35 0.40283779715710466 1104 36-37 0.4528243557995353 1104 38-39 0.45334452832872074 1104 40-41 0.4746768566594568 1104 42-43 0.4545152058016555 1104 44-45 0.37766154360504345 1104 46-47 0.4418602094883113 1104 48-49 0.39335615148721814 1104 50-51 0.3251308727721778 1104 52-53 0.3373330239733576 1104 54-55 0.2913849077695687 1104 56-57 0.4067567070571272 1104 58-59 0.35183686145419557 1104 60-61 0.3394358772260233 1104 62-63 0.33861128244345906 1104 64-65 0.4185731576167768 1104 66-67 0.36638781110449514 1104 68-69 0.3845481779669342 1104 70-71 0.3467336833970691 1104 72-73 0.41263482412723107 1104 74-75 0.36274051237563043 1104 76-77 0.24597005279523643 1104 78-79 0.29930204443678576 1104 80-81 0.2795475550315487 1104 82-83 0.3293011460820452 1104 84-85 0.2944148153576762 1104 86-87 0.3065565770493066 1104 88-89 0.2433688289443836 1104 90-91 0.23385562543712268 1104 92-93 0.27731361408089583 1104 94-95 0.2545950842485283 1104 96-97 0.25116176341268925 1104 98-99 0.20495375555608675 1104 100 0.2265966022859871 1105 1 0.20512545917542369 1105 2 0.22484906912395175 1105 3 0.19878713749905685 1105 4 0.22448357579267508 1105 5 0.22782141639027742 1105 6 0.2031494110161205 1105 7 0.22155381751613845 1105 8 0.2565164167782257 1105 9 0.2570307433075172 1105 10-11 0.38798477136049314 1105 12-13 0.39490011353054655 1105 14-15 0.4534364038422112 1105 16-17 0.39035400980485946 1105 18-19 0.38762851897300266 1105 20-21 0.3585937982716061 1105 22-23 0.3622319538797214 1105 24-25 0.4606152064888889 1105 26-27 0.3213325690691562 1105 28-29 0.36517314642578924 1105 30-31 0.3686348213420487 1105 32-33 0.3965508329717551 1105 34-35 0.37567131583119817 1105 36-37 0.36870982849473677 1105 38-39 0.43469246675910966 1105 40-41 0.4464442276210008 1105 42-43 0.4297545268337615 1105 44-45 0.3346737760949452 1105 46-47 0.33678324485438793 1105 48-49 0.33806897216661724 1105 50-51 0.3704686826020449 1105 52-53 0.38150676081122725 1105 54-55 0.29098631884046 1105 56-57 0.3954437317488271 1105 58-59 0.3622748710207233 1105 60-61 0.4165467004335639 1105 62-63 0.4094990092119062 1105 64-65 0.4241613425117947 1105 66-67 0.35289641340548883 1105 68-69 0.42202433200246503 1105 70-71 0.38713416540578294 1105 72-73 0.35389323481884816 1105 74-75 0.39191874420903616 1105 76-77 0.313410750547952 1105 78-79 0.3564084936375451 1105 80-81 0.26939105804022745 1105 82-83 0.25621193344009185 1105 84-85 0.3000053674241343 1105 86-87 0.2735750075830836 1105 88-89 0.27335609093187685 1105 90-91 0.2286340562181408 1105 92-93 0.2630566994416341 1105 94-95 0.252527886037889 1105 96-97 0.26030415758565795 1105 98-99 0.3606825997504366 1105 100 0.4961352681902511 1106 1 0.21760764848467673 1106 2 0.20010996929187996 1106 3 0.15297577992135203 1106 4 0.1601769684042864 1106 5 0.19379002322141758 1106 6 0.1670948228864546 1106 7 0.23592999870250253 1106 8 0.2273747132095849 1106 9 0.20901128204186392 1106 10-11 0.23508666715359539 1106 12-13 0.2591001622265381 1106 14-15 0.331819600601726 1106 16-17 0.32451255291555725 1106 18-19 0.31887342705523025 1106 20-21 0.3869057585661153 1106 22-23 0.3359561308973369 1106 24-25 0.3623147261544588 1106 26-27 0.3452880268492464 1106 28-29 0.28725559405303613 1106 30-31 0.30792928937173514 1106 32-33 0.4065944597171125 1106 34-35 0.3836405607737916 1106 36-37 0.3849875748805758 1106 38-39 0.3741374407937954 1106 40-41 0.3543034090446753 1106 42-43 0.4026485826983688 1106 44-45 0.3821403375937251 1106 46-47 0.3345538225001832 1106 48-49 0.3087045562388724 1106 50-51 0.3682236727444419 1106 52-53 0.3675721468296089 1106 54-55 0.30443211501360423 1106 56-57 0.32289250019120885 1106 58-59 0.3279978880761689 1106 60-61 0.36251687105342256 1106 62-63 0.24884694080373748 1106 64-65 0.3137432944879279 1106 66-67 0.3381801412359664 1106 68-69 0.35112907586860587 1106 70-71 0.307275795665916 1106 72-73 0.3029954387091891 1106 74-75 0.29279612541273536 1106 76-77 0.33166533875319004 1106 78-79 0.3412273202430356 1106 80-81 0.24698663557266087 1106 82-83 0.23169379827735526 1106 84-85 0.2466656708205619 1106 86-87 0.2123853655452166 1106 88-89 0.28385010205561656 1106 90-91 0.23808894768377087 1106 92-93 0.23778236567986255 1106 94-95 0.1998154211239509 1106 96-97 0.23222084549751543 1106 98-99 0.14466587634316141 1106 100 0.19159673512610098 1107 1 0.10307915282991686 1107 2 0.1591515628753548 1107 3 0.0982976643092961 1107 4 0.10123342931228763 1107 5 0.16807756547640906 1107 6 0.22486556168448857 1107 7 0.25151570171133386 1107 8 0.2660914753085777 1107 9 0.30550190830253854 1107 10-11 0.27794573464521477 1107 12-13 0.2584817043446819 1107 14-15 0.3427058035321906 1107 16-17 0.2923268905364509 1107 18-19 0.31726462336099104 1107 20-21 0.32907158702339245 1107 22-23 0.3303064444122654 1107 24-25 0.43994983630759776 1107 26-27 0.3514640336752848 1107 28-29 0.3616863568556852 1107 30-31 0.3810123680612634 1107 32-33 0.373935790239031 1107 34-35 0.3811418021955433 1107 36-37 0.35113457651138447 1107 38-39 0.33593235837598456 1107 40-41 0.3707135253309559 1107 42-43 0.3673279833507195 1107 44-45 0.3122487406800616 1107 46-47 0.3164004056812004 1107 48-49 0.36901624602437977 1107 50-51 0.39804281280518694 1107 52-53 0.35991392870865724 1107 54-55 0.2973407711026894 1107 56-57 0.34352860879182856 1107 58-59 0.343660890734391 1107 60-61 0.3070164358541234 1107 62-63 0.3659779389037894 1107 64-65 0.3711110737789909 1107 66-67 0.31607207738812804 1107 68-69 0.3803542299482743 1107 70-71 0.3217637677949483 1107 72-73 0.3177335613032497 1107 74-75 0.35488082185724323 1107 76-77 0.3212903603538493 1107 78-79 0.259210950923368 1107 80-81 0.2393185255240482 1107 82-83 0.31586545477924943 1107 84-85 0.26768114698413115 1107 86-87 0.28178523352136153 1107 88-89 0.2992125994821251 1107 90-91 0.31259740459919527 1107 92-93 0.3340123881961361 1107 94-95 0.28226612496039394 1107 96-97 0.28703499028651436 1107 98-99 0.3661974319716741 1107 100 0.36607647207364735 1108 1 -0.07151381400612067 1108 2 -0.035855364725168926 1108 3 -0.04697716207236624 1108 4 -0.08861050843233897 1108 5 -0.09527143755477852 1108 6 -0.0840128733916714 1108 7 -0.01756946576917784 1108 8 -0.01610520001427318 1108 9 -0.044763044801840124 1108 10-11 -0.05189571430312867 1108 12-13 -0.05648805963597425 1108 14-15 -0.10855789931274273 1108 16-17 -0.07572398370584921 1108 18-19 -0.11182880403762141 1108 20-21 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-0.44485906558508503 2215 3 -0.4314717218942121 2215 4 -0.38255270339063685 2215 5 -0.28586237907916257 2215 6 -0.3747842591754633 2215 7 -0.4555593629996224 2215 8 -0.4554244103126308 2215 9 -0.6144393923780385 2215 10-11 -0.5584041344819468 2215 12-13 -0.5737312338810199 2215 14-15 -0.6691815099280731 2215 16-17 -0.7112808662664207 2215 18-19 -0.7498976608874628 2215 20-21 -0.7093840966106342 2215 22-23 -0.6394167348786723 2215 24-25 -0.6478605634881163 2215 26-27 -0.702851880093732 2215 28-29 -0.6594929398938731 2215 30-31 -0.7040972077095589 2215 32-33 -0.715309446879715 2215 34-35 -0.7047940111119004 2215 36-37 -0.6716930843761162 2215 38-39 -0.8149758681375161 2215 40-41 -0.7839036111753046 2215 42-43 -0.7675149378121802 2215 44-45 -0.6692132505796025 2215 46-47 -0.6443331254284175 2215 48-49 -0.6313353235790728 2215 50-51 -0.6343033197236281 2215 52-53 -0.5982417920688832 2215 54-55 -0.6467040071389576 2215 56-57 -0.638916331275567 2215 58-59 -0.5716657520920734 2215 60-61 -0.5557044543713303 2215 62-63 -0.6443339311558347 2215 64-65 -0.6458996983223599 2215 66-67 -0.6591539515064255 2215 68-69 -0.6512774703925928 2215 70-71 -0.5769122934067212 2215 72-73 -0.5486885732297679 2215 74-75 -0.49361302818357444 2215 76-77 -0.525202455665827 2215 78-79 -0.49528078996583247 2215 80-81 -0.5258063429260247 2215 82-83 -0.4785461277083627 2215 84-85 -0.48828348156143875 2215 86-87 -0.49803562401006474 2215 88-89 -0.4842023399468687 2215 90-91 -0.4885200564291452 2215 92-93 -0.48481397847220364 2215 94-95 -0.5732005725947147 2215 96-97 -0.5058522694897789 2215 98-99 -0.43560395568662 2215 100 -0.4276142820943569 2216 1 -0.32783702889846467 2216 2 -0.39328566415231236 2216 3 -0.3401872207881169 2216 4 -0.27809173673651344 2216 5 -0.3951300874100028 2216 6 -0.2674021878867592 2216 7 -0.43217614546888683 2216 8 -0.43373261879565916 2216 9 -0.4774392779335912 2216 10-11 -0.5951136514065496 2216 12-13 -0.4924987825289051 2216 14-15 -0.6767663381295819 2216 16-17 -0.6754987013849316 2216 18-19 -0.6598162495417554 2216 20-21 -0.6786257658338215 2216 22-23 -0.6853234135210684 2216 24-25 -0.689670685170718 2216 26-27 -0.7092479989889284 2216 28-29 -0.7305307353223256 2216 30-31 -0.6977481997811381 2216 32-33 -0.7869533934275665 2216 34-35 -0.7523985570131089 2216 36-37 -0.6902607222427264 2216 38-39 -0.5936407568208182 2216 40-41 -0.696314287583931 2216 42-43 -0.6561418036761069 2216 44-45 -0.5296160785558186 2216 46-47 -0.6614421957799976 2216 48-49 -0.5522275094200779 2216 50-51 -0.6112664689112535 2216 52-53 -0.5448029386584352 2216 54-55 -0.5587204194313671 2216 56-57 -0.48118151194201175 2216 58-59 -0.4357091567963991 2216 60-61 -0.5432594820776728 2216 62-63 -0.5061518482429292 2216 64-65 -0.4839015986394486 2216 66-67 -0.434166129953482 2216 68-69 -0.46846568567038815 2216 70-71 -0.4948401209035893 2216 72-73 -0.44298470842282 2216 74-75 -0.36106838286549703 2216 76-77 -0.4757838005019295 2216 78-79 -0.3930377462109078 2216 80-81 -0.4214322207329033 2216 82-83 -0.408761116994917 2216 84-85 -0.4098551813071367 2216 86-87 -0.4526791100837997 2216 88-89 -0.4571700821916167 2216 90-91 -0.4023100955080281 2216 92-93 -0.3302455928219814 2216 94-95 -0.4130019395767093 2216 96-97 -0.41240942700128613 2216 98-99 -0.38051026403231347 2216 100 -0.4252156672915959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1143.0 1 289.0 2 196.0 3 207.0 4 166.0 5 232.0 6 384.0 7 1040.0 8 2913.0 9 4155.0 10 4239.0 11 4908.0 12 5962.0 13 7502.0 14 9374.0 15 10451.0 16 11197.0 17 12547.0 18 14013.0 19 15593.0 20 17469.0 21 19108.0 22 21311.0 23 24459.0 24 29986.0 25 36324.0 26 39302.0 27 38185.0 28 41501.0 29 50256.0 30 58843.0 31 67370.0 32 84352.0 33 112414.0 34 163459.0 35 246621.0 36 451229.0 37 898973.0 38 1052298.0 39 315557.0 40 405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.968706920106214 20.17439334987188 13.288505709724221 24.568394020297685 2 14.913400780074538 20.68945309071232 40.48304417014588 23.914101959067256 3 17.555593884491962 26.37276692345906 30.66055274228048 25.411086449768494 4 12.37304744235767 17.78434493861684 39.73068615641635 30.111921462609143 5 13.09231356359781 38.47729123620967 35.65968175144636 12.770713448746159 6 29.655316133657124 39.02009980178057 18.405691862260735 12.918892202301576 7 26.013654226682682 33.151666031011864 23.377317891354416 17.45736185095104 8 25.249229574227105 35.55268978916647 21.124850033595575 18.073230603010845 9 26.09791714861694 15.628743720876434 20.957039641098515 37.31629948940811 10-11 24.174614578713864 26.614883397271665 29.20701623923845 20.00348578477603 12-13 24.638183118482505 24.687650875367588 28.404897204658646 22.26926880149126 14-15 22.269347159249353 25.93472108899745 25.859067576918708 25.93686417483449 16-17 21.996706059087728 28.634370105775602 26.681734740025387 22.687189095111286 18-19 21.945923140074306 28.518337726493474 27.796200059568125 21.73953907386409 20-21 23.31077875579544 27.590924552646744 27.1105978974736 21.987698794084213 22-23 23.15607581904384 27.53440936216084 26.77788428333059 22.531630535464735 24-25 22.852258055270852 27.68141198991827 27.104306012052064 22.362023942758814 26-27 22.62665859059044 27.840207292841416 26.90404857212494 22.629085544443207 28-29 22.483752411526993 28.03644541405449 26.963721777404004 22.516080397014516 30-31 22.89909792942603 27.753208013332486 26.83913014512558 22.508563912115903 32-33 22.80392633816521 27.997056769894048 26.496352852955212 22.702664038985535 34-35 22.939530555057384 27.961274207332483 26.88090242257637 22.218292815033767 36-37 22.996637057033684 27.699721704397234 26.747055714123768 22.556585524445314 38-39 22.799116068609912 27.73230797636394 27.01285806419542 22.45571789083073 40-41 22.82539284138202 27.379391052928266 27.251880445765515 22.543335659924203 42-43 22.68981616039254 27.453653389497973 26.960925900556614 22.89560454955287 44-45 22.64979904050216 27.819885442305214 26.96895957220914 22.56135594498349 46-47 22.673660307473337 27.604391399092254 26.797749661021193 22.924198632413216 48-49 22.80714825071817 27.363821077324978 27.075041602837775 22.753989069119072 50-51 22.642274632976168 27.416647761833445 26.92441626028269 23.016661344907696 52-53 22.53274337814381 27.67839238545128 26.84182914343687 22.947035092968044 54-55 22.472783584858743 27.483472865959076 26.88423272291199 23.159510826270196 56-57 22.844213368619656 27.4430303058475 26.809844325962562 22.902911999570282 58-59 22.53049742244113 27.466203395175032 27.12706552382817 22.87623365855567 60-61 22.624963393035614 27.519837488320636 26.919095897075934 22.936103221567816 62-63 22.62381037665719 27.296021635349476 26.80591031179711 23.27425767619622 64-65 22.294751437825592 27.375613026540393 26.94304184611912 23.386593689514896 66-67 22.652101430654145 27.189737616557828 26.799487603967453 23.35867334882057 68-69 22.279275164641106 27.181377609687885 27.043404613140375 23.495942612530634 70-71 22.31468477649469 26.95859949102265 27.066203211815466 23.660512520667197 72-73 22.465398663335893 27.006642516599833 27.0246814647161 23.503277355348168 74-75 22.316308744747907 27.266790753266957 27.02868549212079 23.38821500986434 76-77 22.276313793092317 27.075265276607556 26.989924815428175 23.658496114871955 78-79 22.236325306455832 27.125181193926736 26.896326404111694 23.742167095505746 80-81 22.155256304435188 27.09264583283833 27.161718021216746 23.590379841509737 82-83 22.407707888485625 26.68784071810123 27.53298625795883 23.37146513545431 84-85 22.524449368645538 26.91096988467943 27.022113063358844 23.54246768331619 86-87 22.01713021231407 27.101405428410906 27.24345776115658 23.63800659811844 88-89 22.410255277922076 26.532016817866577 27.19231473959901 23.865413164612338 90-91 22.320569303443637 26.550961534364255 27.25152469396686 23.87694446822525 92-93 22.49700249484362 26.555390770602216 27.210761734299798 23.736845000254363 94-95 22.032185832666002 26.499546662045915 27.423305057061704 24.04496244822638 96-97 22.054733765055786 26.506463247963673 27.306365635908325 24.132437351072216 98-99 21.950217617458158 26.68124078625348 27.097157181479425 24.27138441480894 100 22.368663351330774 26.385913167442393 26.851246458014998 24.394177023211835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1734.0 1 1136.5 2 566.5 3 589.0 4 555.5 5 513.5 6 479.0 7 465.5 8 513.0 9 558.0 10 509.5 11 472.5 12 483.0 13 495.5 14 558.0 15 628.5 16 686.0 17 822.5 18 1048.0 19 1400.5 20 1864.5 21 2476.0 22 3629.0 23 5612.0 24 8199.0 25 11363.0 26 15775.5 27 21220.0 28 27029.0 29 34453.5 30 43121.0 31 51810.0 32 62439.5 33 72859.0 34 81418.5 35 88902.0 36 97084.0 37 108104.5 38 113607.0 39 113789.5 40 116113.5 41 118720.5 42 121079.5 43 127603.5 44 135668.5 45 145800.5 46 155002.5 47 160684.0 48 164334.5 49 161620.5 50 154316.0 51 151589.0 52 150598.0 53 145779.5 54 139208.0 55 126395.5 56 113568.0 57 99166.5 58 84871.0 59 75380.0 60 61555.0 61 47751.0 62 38096.0 63 29309.5 64 21054.5 65 14392.5 66 10015.0 67 7147.5 68 5372.0 69 3942.0 70 2899.0 71 2168.5 72 1621.5 73 1292.0 74 1063.0 75 758.0 76 502.0 77 326.5 78 222.5 79 151.5 80 115.5 81 91.5 82 63.0 83 56.0 84 45.0 85 40.5 86 40.0 87 34.0 88 28.0 89 28.0 90 23.0 91 16.0 92 15.0 93 13.0 94 13.5 95 12.5 96 10.0 97 8.5 98 9.5 99 11.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09718950250172023 2 0.06339118865057781 3 0.07804572473259987 4 0.08070314940944542 5 0.08026454533656799 6 0.08919142823160256 7 0.08477958726324733 8 0.08769501433590313 9 0.07175046627482982 10-11 0.07896163323772624 12-13 0.0697380475875099 14-15 0.07773612185762757 16-17 0.07906483419605034 18-19 0.07885843227940215 20-21 0.07057655537389321 22-23 0.07470459370685716 24-25 0.07707821574831143 26-27 0.07133766244153343 28-29 0.0806902492896549 30-31 0.08480538750282836 32-33 0.08557939469025909 34-35 0.08832712020563822 36-37 0.08854642224207694 38-39 0.08928172907013615 40-41 0.08550199397151602 42-43 0.09052014057002533 44-45 0.08737251134114031 46-47 0.08441838390911298 48-49 0.09290666273127013 50-51 0.08943653050762229 52-53 0.09446757722592211 54-55 0.08674040547140521 56-57 0.09316466512708037 58-59 0.0970476011840246 60-61 0.09595109100183104 62-63 0.09678959878821435 64-65 0.09942122322547887 66-67 0.10134334107426521 68-69 0.09800221004852251 70-71 0.0994599235848504 72-73 0.09690569986632896 74-75 0.10077573580348267 76-77 0.09792480932977944 78-79 0.10437486922503561 80-81 0.10054353364725346 82-83 0.10463287162084586 84-85 0.10063383448578704 86-87 0.09722820286109177 88-89 0.09653159639240409 90-91 0.09707340142360563 92-93 0.09148764955431375 94-95 0.09148764955431375 96-97 0.08928172907013615 98-99 0.10322675856368002 100 0.24399286571775103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3875933.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.66301672104408 #Duplication Level Percentage of deduplicated Percentage of total 1 68.07600817511549 37.21239973178271 2 15.84235678470721 17.319820276463926 3 6.6648048709397 10.929550203080186 4 3.443021416172936 7.528237489726962 5 1.9166945600878555 5.238615339360833 6 1.1455342977777558 3.7571016284372973 7 0.7423562726722485 2.8405603342238552 8 0.5265742201568008 2.3027308321041557 9 0.3747128395220484 1.8434640791145274 >10 1.2492438786048148 10.17307757430655 >50 0.014800438320209391 0.5279445718925598 >100 0.003892245923010253 0.32649793950646117 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.5800239581024748E-5 0.0 6 0.0 0.0 0.0 2.5800239581024748E-5 0.0 7 0.0 0.0 0.0 7.740071874307425E-5 0.0 8 0.0 0.0 0.0 7.740071874307425E-5 0.0 9 0.0 0.0 0.0 7.740071874307425E-5 0.0 10-11 0.0 0.0 0.0 1.0320095832409899E-4 0.0 12-13 0.0 0.0 0.0 1.0320095832409899E-4 0.0 14-15 0.0 0.0 0.0 1.0320095832409899E-4 1.2900119790512374E-5 16-17 0.0 0.0 0.0 1.0320095832409899E-4 2.5800239581024748E-5 18-19 0.0 0.0 0.0 1.1610107811461137E-4 2.5800239581024748E-5 20-21 0.0 0.0 0.0 1.2900119790512375E-4 7.740071874307425E-5 22-23 0.0 0.0 0.0 2.193020364387104E-4 7.740071874307425E-5 24-25 0.0 0.0 0.0 4.386040728774207E-4 7.740071874307425E-5 26-27 0.0 0.0 0.0 9.804091040789406E-4 7.740071874307425E-5 28-29 0.0 0.0 0.0 0.0034185317444857793 7.740071874307425E-5 30-31 0.0 0.0 0.0 0.011777809368737797 7.740071874307425E-5 32-33 0.0 0.0 0.0 0.03501092511145058 7.740071874307425E-5 34-35 0.0 0.0 0.0 0.07100225932698011 1.2900119790512375E-4 36-37 0.0 0.0 0.0 0.1305234120404042 1.2900119790512375E-4 38-39 0.0 0.0 0.0 0.2206694491365047 1.2900119790512375E-4 40-41 0.0 0.0 0.0 0.32023257367967917 1.2900119790512375E-4 42-43 0.0 0.0 0.0 0.42589745488376607 1.2900119790512375E-4 44-45 0.0 0.0 0.0 0.5591169919603873 1.548014374861485E-4 46-47 0.0 0.0 0.0 0.7230646143780091 1.8060167706717326E-4 48-49 0.0 0.0 0.0 0.8982611412529578 1.8060167706717326E-4 50-51 0.0 0.0 0.0 1.085712781928893 1.8060167706717326E-4 52-53 0.0 0.0 0.0 1.2743512336255556 2.0640191664819798E-4 54-55 0.0 0.0 0.0 1.4848682884869269 2.0640191664819798E-4 56-57 0.0 0.0 0.0 1.7375687350632738 2.0640191664819798E-4 58-59 0.0 0.0 0.0 2.0204038614702577 2.0640191664819798E-4 60-61 0.0 0.0 0.0 2.3145523929335208 2.0640191664819798E-4 62-63 0.0 0.0 0.0 2.6168925004637593 2.0640191664819798E-4 64-65 0.0 0.0 0.0 2.936338166836217 2.1930203643871036E-4 66-67 0.0 0.0 0.0 3.307616514526954 2.3220215622922274E-4 68-69 0.0 0.0 0.0 3.7137380857718645 2.3220215622922274E-4 70-71 0.0 0.0 0.0 4.153116165836716 2.3220215622922274E-4 72-73 0.0 0.0 0.0 4.605239564254593 2.580023958102475E-4 74-75 0.0 0.0 0.0 5.059039978245238 2.9670275518178465E-4 76-77 0.0 0.0 2.5800239581024748E-5 5.562892857023071 3.09602874972297E-4 78-79 0.0 0.0 2.5800239581024748E-5 6.113031365609261 3.09602874972297E-4 80-81 0.0 0.0 2.5800239581024748E-5 6.70844929466015 3.09602874972297E-4 82-83 0.0 0.0 2.5800239581024748E-5 7.321217884829278 3.3540311455332176E-4 84-85 0.0 0.0 2.5800239581024748E-5 7.93507008506081 3.3540311455332176E-4 86-87 0.0 0.0 2.5800239581024748E-5 8.576489841284666 3.7410347392485886E-4 88 0.0 0.0 2.5800239581024748E-5 9.084315956957976 4.1280383329639597E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3970 0.0 38.854473 1 GTATCAA 5945 0.0 34.88546 1 CAACGCA 5980 0.0 32.38851 5 ATCAACG 6280 0.0 30.842886 3 AACGCAG 7130 0.0 28.076023 6 TATCAAC 7245 0.0 27.96767 2 TCAACGC 7305 0.0 26.707897 4 GTACATG 11715 0.0 25.611563 1 ACGCAGA 7590 0.0 25.50801 7 TACATGG 11675 0.0 25.409136 2 CGCAGAG 7760 0.0 24.948877 8 ACATGGG 12155 0.0 23.748207 3 GTACTAG 1730 0.0 22.290789 1 CATGGGG 9475 0.0 21.03771 4 CTGTGCG 2310 0.0 20.545359 9 TGATCGC 1025 0.0 20.171265 9 GCAGAGT 9465 0.0 19.361916 9 CTAGTAC 2685 0.0 19.260424 3 GTATTAG 810 0.0 19.159609 1 TAGTACT 2680 0.0 18.94527 4 >>END_MODULE