FastQCFastQC Report
Mon 16 Jan 2017
ERR1146609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875933
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110720.28566025264110606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103910.26809028948642816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56940.14690656417435494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72850.059.453961
GTATCAA132400.043.4399381
ATCAACG161450.034.77633
CAACGCA162950.034.410445
TATCAAC170550.033.6122132
AACGCAG175100.032.3440066
TCAACGC176050.031.9189784
ACGCAGA191600.029.2880547
CGCAGAG192150.029.0330078
GCAGAGT217200.025.446249
GTGGTAT19250.024.91841
GTACATG114500.024.8485241
TACATGG117050.024.3872242
GAGTACT153200.023.31490912-13
ACATGGG119850.023.2666443
CAGAGTA211100.022.73074210-11
CATGGGG93800.022.5092264
AGAGTAC203650.021.84310-11
GTACTTT167900.021.06383914-15
TGATCGC9450.020.3905129
Produced by FastQC (version 0.11.5)