##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3875933
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.347550641355255	33.0	31.0	34.0	30.0	34.0
2	32.40848409918335	34.0	31.0	34.0	30.0	34.0
3	32.51677080073365	34.0	31.0	34.0	30.0	34.0
4	36.030954869446916	37.0	35.0	37.0	35.0	37.0
5	36.07525517081952	37.0	35.0	37.0	35.0	37.0
6	36.14180430879481	37.0	36.0	37.0	35.0	37.0
7	36.10501548917383	37.0	36.0	37.0	35.0	37.0
8	36.10667444457889	37.0	36.0	37.0	35.0	37.0
9	37.88936367063105	39.0	38.0	39.0	35.0	39.0
10-11	37.839941118693226	39.0	38.0	39.0	35.0	39.0
12-13	37.81347652294299	39.0	38.0	39.0	35.0	39.0
14-15	39.160709305346614	40.0	39.0	41.0	36.0	41.0
16-17	39.105794269405585	40.0	39.0	41.0	36.0	41.0
18-19	39.21624509505195	40.0	39.0	41.0	36.0	41.0
20-21	39.27730742507675	41.0	39.0	41.0	36.0	41.0
22-23	39.244121221909666	40.5	39.0	41.0	36.0	41.0
24-25	39.2350788829425	41.0	39.0	41.0	36.0	41.0
26-27	39.15444010513082	41.0	39.0	41.0	35.5	41.0
28-29	39.09312170772818	40.0	39.0	41.0	35.0	41.0
30-31	38.948262005560984	40.0	38.5	41.0	35.0	41.0
32-33	38.8340682101574	40.0	38.0	41.0	35.0	41.0
34-35	38.76103934201133	40.0	38.0	41.0	35.0	41.0
36-37	38.68702671073004	40.0	38.0	41.0	35.0	41.0
38-39	38.594878833044845	40.0	38.0	41.0	35.0	41.0
40-41	38.49444146738347	40.0	38.0	41.0	34.5	41.0
42-43	38.38679177374841	40.0	38.0	41.0	34.0	41.0
44-45	38.227108543929944	40.0	38.0	41.0	34.0	41.0
46-47	37.97118513142513	40.0	38.0	41.0	33.0	41.0
48-49	37.67442110067434	40.0	37.0	41.0	33.0	41.0
50-51	37.55725098963269	40.0	37.0	41.0	33.0	41.0
52-53	37.45812917818755	40.0	37.0	41.0	32.5	41.0
54-55	37.296769190798706	40.0	36.0	41.0	32.0	41.0
56-57	37.14181308087627	39.5	36.0	41.0	32.0	41.0
58-59	36.952628567108874	39.0	35.5	41.0	32.0	41.0
60-61	36.749449745390336	39.0	35.0	41.0	31.0	41.0
62-63	36.508302259094776	39.0	35.0	41.0	31.0	41.0
64-65	36.23363600454394	38.0	35.0	40.5	31.0	41.0
66-67	35.96549463058314	37.0	35.0	40.0	31.0	41.0
68-69	35.67297422323864	37.0	35.0	40.0	30.5	41.0
70-71	35.37066288297553	36.0	35.0	39.0	30.0	41.0
72-73	35.048560565933414	36.0	35.0	39.0	30.0	41.0
74-75	34.735883592415036	35.5	35.0	38.5	30.0	40.5
76-77	34.44158993460414	35.0	34.0	37.0	30.0	39.5
78-79	34.162064591931795	35.0	34.0	37.0	29.0	39.0
80-81	33.90401240165916	35.0	34.0	36.5	29.0	39.0
82-83	33.649044501027234	35.0	34.0	36.0	29.0	37.5
84-85	33.441062190703505	35.0	34.0	36.0	29.0	37.0
86-87	33.257027920761274	35.0	34.0	35.0	29.0	37.0
88-89	33.06670548742716	35.0	34.0	35.0	29.0	36.0
90-91	32.9829486216609	35.0	34.0	35.0	29.0	36.0
92-93	32.887163168197176	35.0	34.0	35.0	29.0	36.0
94-95	32.81361997227506	35.0	34.0	35.0	29.0	36.0
96-97	32.75905040154203	35.0	34.0	35.0	29.0	35.5
98-99	32.691537753619585	35.0	34.0	35.0	29.0	35.0
100	31.953035823890662	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3019156667980809
1101	2	0.2960541792685021
1101	3	0.31506073173273563
1101	4	0.20806513399832482
1101	5	0.20091202320065804
1101	6	0.1843560477178201
1101	7	0.19500010021824465
1101	8	0.19140759118829465
1101	9	0.24337358769239614
1101	10-11	0.2806074598866104
1101	12-13	0.2784144418231378
1101	14-15	0.4281462973523631
1101	16-17	0.42734012859702375
1101	18-19	0.39103778131674005
1101	20-21	0.36387776412691153
1101	22-23	0.31631390262793246
1101	24-25	0.3486156836198262
1101	26-27	0.38434634252885047
1101	28-29	0.4028974960556724
1101	30-31	0.46354071339222713
1101	32-33	0.4216427155977769
1101	34-35	0.4124700547890825
1101	36-37	0.40673198356495277
1101	38-39	0.4306482721102114
1101	40-41	0.42791552597098814
1101	42-43	0.4761980695093939
1101	44-45	0.4571006339213284
1101	46-47	0.48627599185063985
1101	48-49	0.5483418579641253
1101	50-51	0.5331803884642099
1101	52-53	0.4940015105702926
1101	54-55	0.4788194498274265
1101	56-57	0.4274705921295734
1101	58-59	0.39987325983568667
1101	60-61	0.42453884526162966
1101	62-63	0.4209598029312218
1101	64-65	0.39891153036487026
1101	66-67	0.34942424759113777
1101	68-69	0.37748967272688105
1101	70-71	0.4220926604820363
1101	72-73	0.4239723816757106
1101	74-75	0.3490544220311307
1101	76-77	0.2908577086255377
1101	78-79	0.3298335400901635
1101	80-81	0.300825250513185
1101	82-83	0.294862577883805
1101	84-85	0.28847221704754133
1101	86-87	0.31792946510618236
1101	88-89	0.3484387460431577
1101	90-91	0.3617261072700302
1101	92-93	0.3304238623383995
1101	94-95	0.33021792831561925
1101	96-97	0.2840321638201786
1101	98-99	0.37501517381446803
1101	100	0.3094863891444355
1102	1	0.25098964749614083
1102	2	0.25865918526884
1102	3	0.26813578139944383
1102	4	0.18870079217038693
1102	5	0.15754252813707836
1102	6	0.2024509892775086
1102	7	0.20241922164703396
1102	8	0.21727587609868948
1102	9	0.23943806868794582
1102	10-11	0.24587858462315637
1102	12-13	0.2964863778665432
1102	14-15	0.4107286003747177
1102	16-17	0.47230362230926914
1102	18-19	0.41118147440172237
1102	20-21	0.3466708985245077
1102	22-23	0.37379901522260184
1102	24-25	0.3338102157443501
1102	26-27	0.35648729259994383
1102	28-29	0.3733788975139021
1102	30-31	0.38450731064315846
1102	32-33	0.38180377942327937
1102	34-35	0.4466137075356187
1102	36-37	0.41891324686403664
1102	38-39	0.39097604714272904
1102	40-41	0.4163100308670593
1102	42-43	0.4730195265338324
1102	44-45	0.3955971194347896
1102	46-47	0.4104829003126369
1102	48-49	0.49479699153477696
1102	50-51	0.4019950560250436
1102	52-53	0.349859345303706
1102	54-55	0.46547278379945567
1102	56-57	0.36097078373715163
1102	58-59	0.3842878104150458
1102	60-61	0.35949909929295387
1102	62-63	0.3568995246968285
1102	64-65	0.3886331068919304
1102	66-67	0.3243063206843857
1102	68-69	0.35263709711751545
1102	70-71	0.318714379202099
1102	72-73	0.30133407125124734
1102	74-75	0.26790797836880387
1102	76-77	0.31989571247785165
1102	78-79	0.3497909830364847
1102	80-81	0.271872020412637
1102	82-83	0.2780326245755518
1102	84-85	0.27201224286415027
1102	86-87	0.27333847167126635
1102	88-89	0.3478254757050223
1102	90-91	0.28698118487110236
1102	92-93	0.3252435243022731
1102	94-95	0.25450299250416464
1102	96-97	0.2540997811294048
1102	98-99	0.30660401670633064
1102	100	0.3716422518323874
1103	1	0.29634771226071877
1103	2	0.2692541919147473
1103	3	0.22546860690570014
1103	4	0.22140239523905336
1103	5	0.18720803619559945
1103	6	0.21022797870124776
1103	7	0.2053250534701121
1103	8	0.22069400838424258
1103	9	0.2341229513898142
1103	10-11	0.26510418383381307
1103	12-13	0.3132032802225453
1103	14-15	0.43474939463882123
1103	16-17	0.5073485404987395
1103	18-19	0.44127529744684324
1103	20-21	0.4078576909751348
1103	22-23	0.3811312426560036
1103	24-25	0.3559726444569833
1103	26-27	0.3402575643525907
1103	28-29	0.3707842823869214
1103	30-31	0.440471176539468
1103	32-33	0.4193761754140297
1103	34-35	0.43062317196574895
1103	36-37	0.38749108210867433
1103	38-39	0.4043261639565756
1103	40-41	0.42819158705848537
1103	42-43	0.37476946585131543
1103	44-45	0.36521691430454695
1103	46-47	0.4881898874957855
1103	48-49	0.5579259162056047
1103	50-51	0.4443028762507595
1103	52-53	0.41529482549494645
1103	54-55	0.42285732072767956
1103	56-57	0.43412257083722494
1103	58-59	0.48344759827130446
1103	60-61	0.46766925598220865
1103	62-63	0.4540178783568649
1103	64-65	0.4476771939059887
1103	66-67	0.3935194333006322
1103	68-69	0.39013925601467747
1103	70-71	0.3963692538168502
1103	72-73	0.36839765894156784
1103	74-75	0.3271336753133198
1103	76-77	0.3070340826155231
1103	78-79	0.3890804316308163
1103	80-81	0.3750430173213317
1103	82-83	0.3063876966418704
1103	84-85	0.3336556858266704
1103	86-87	0.33822354248892594
1103	88-89	0.4038869295880687
1103	90-91	0.35560979066697485
1103	92-93	0.3435736552714772
1103	94-95	0.363398859558103
1103	96-97	0.36510188576239955
1103	98-99	0.3524690211583277
1103	100	0.36270390701799826
1104	1	0.1941625944018952
1104	2	0.1691332028352477
1104	3	0.169874837635696
1104	4	0.18122905921430998
1104	5	0.1423114965981398
1104	6	0.11091922327571524
1104	7	0.12638576868829432
1104	8	0.15251627456838435
1104	9	0.20806943676569745
1104	10-11	0.22608119210271127
1104	12-13	0.178434116162002
1104	14-15	0.28939636761786147
1104	16-17	0.40053972850229513
1104	18-19	0.2819162136367126
1104	20-21	0.27862232064398995
1104	22-23	0.329618917222227
1104	24-25	0.2688890694718822
1104	26-27	0.2795894020918652
1104	28-29	0.2947962170143441
1104	30-31	0.3144281180180286
1104	32-33	0.29899752540553237
1104	34-35	0.2998490037847006
1104	36-37	0.29437313872372783
1104	38-39	0.3695151789453348
1104	40-41	0.29840128554697287
1104	42-43	0.28533233356652943
1104	44-45	0.27325671960097964
1104	46-47	0.3942978222937157
1104	48-49	0.5114694035933383
1104	50-51	0.4035460986928925
1104	52-53	0.3602945317414381
1104	54-55	0.32389728082425506
1104	56-57	0.41028725853710313
1104	58-59	0.3307968205441796
1104	60-61	0.35434639928084266
1104	62-63	0.3216301245498485
1104	64-65	0.26665043230353547
1104	66-67	0.3154013954578474
1104	68-69	0.32000541444048736
1104	70-71	0.354216247472813
1104	72-73	0.3161111078908405
1104	74-75	0.3292935594339639
1104	76-77	0.4146440216843317
1104	78-79	0.3903226387722256
1104	80-81	0.4229985032495023
1104	82-83	0.4160884237458262
1104	84-85	0.34579817240413746
1104	86-87	0.3405955069581026
1104	88-89	0.3642684036438837
1104	90-91	0.34901107990236824
1104	92-93	0.3535644579147217
1104	94-95	0.33267540797301365
1104	96-97	0.3234593669723793
1104	98-99	0.26421512041269324
1104	100	0.3641283520574099
1105	1	0.14600370801402818
1105	2	0.1406905530866709
1105	3	0.144245209486769
1105	4	0.12513682790272895
1105	5	0.06418645550141377
1105	6	0.08498301354853766
1105	7	0.11907295145444152
1105	8	0.14237090657054097
1105	9	0.17390918031160396
1105	10-11	0.13964543950264385
1105	12-13	0.13805383730394993
1105	14-15	0.23256947478812862
1105	16-17	0.2952170547611246
1105	18-19	0.259269665340895
1105	20-21	0.17227168626835265
1105	22-23	0.19846030976605533
1105	24-25	0.20882324500973937
1105	26-27	0.1758043705848138
1105	28-29	0.15099262359549925
1105	30-31	0.23355689503731725
1105	32-33	0.22726726643171702
1105	34-35	0.18658006430906937
1105	36-37	0.20706570811999114
1105	38-39	0.21958659519244605
1105	40-41	0.15005643811643665
1105	42-43	0.22825840009561915
1105	44-45	0.23085481058242863
1105	46-47	0.2954367046363515
1105	48-49	0.3654058518787977
1105	50-51	0.370644980178092
1105	52-53	0.3451843339931955
1105	54-55	0.3325088532415563
1105	56-57	0.29235035562322054
1105	58-59	0.2696136418485082
1105	60-61	0.3308530118427413
1105	62-63	0.28904643714903955
1105	64-65	0.3298079141696917
1105	66-67	0.2756084710121698
1105	68-69	0.27720984809997873
1105	70-71	0.2820286805673433
1105	72-73	0.2473809921308998
1105	74-75	0.2733098563572156
1105	76-77	0.26028904105042017
1105	78-79	0.2586019267323252
1105	80-81	0.2501730263828179
1105	82-83	0.21489030951349974
1105	84-85	0.1979150877903777
1105	86-87	0.1961894190929101
1105	88-89	0.21127101226680622
1105	90-91	0.18200688856232006
1105	92-93	0.2052176542800055
1105	94-95	0.19445748432954701
1105	96-97	0.16972114956819695
1105	98-99	0.16602461913520727
1105	100	0.10319724752995185
1106	1	0.15855492319689546
1106	2	0.10563879249863106
1106	3	0.11573701984397644
1106	4	0.0809227328193387
1106	5	0.04115999717868135
1106	6	0.05302091656265162
1106	7	0.10229144529980516
1106	8	0.09607891855988271
1106	9	0.13732131062641173
1106	10-11	0.09940190664758575
1106	12-13	0.1427206570955306
1106	14-15	0.21188493650823403
1106	16-17	0.3071961770795042
1106	18-19	0.2109546555639099
1106	20-21	0.21590806753906122
1106	22-23	0.19869152439974158
1106	24-25	0.1892384444567341
1106	26-27	0.21018915922204684
1106	28-29	0.21776007265617636
1106	30-31	0.19890612691033738
1106	32-33	0.13234708578546162
1106	34-35	0.15396563817859032
1106	36-37	0.181739604953016
1106	38-39	0.22403382012559803
1106	40-41	0.1943775988797114
1106	42-43	0.16617376769870873
1106	44-45	0.10460681837882646
1106	46-47	0.20499783674633676
1106	48-49	0.24247643914790018
1106	50-51	0.15040086287037724
1106	52-53	0.15695834995883473
1106	54-55	0.1557700562164186
1106	56-57	0.14202102474089173
1106	58-59	0.1676898384775285
1106	60-61	0.09309439784197338
1106	62-63	0.08765321664866121
1106	64-65	0.1372390470051954
1106	66-67	0.14511176624187527
1106	68-69	0.17334051962096453
1106	70-71	0.16385073249495719
1106	72-73	0.16284780416336275
1106	74-75	0.13980887286447796
1106	76-77	0.14126489622809402
1106	78-79	0.11375791701932059
1106	80-81	0.06552642122139218
1106	82-83	0.013369885538445203
1106	84-85	0.03551695133782573
1106	86-87	0.02495532527414923
1106	88-89	0.0908012131018694
1106	90-91	0.05831628608233075
1106	92-93	0.09776790776115263
1106	94-95	0.12226835519926738
1106	96-97	0.09647503025824022
1106	98-99	0.04718218681457387
1106	100	0.12478310563996686
1107	1	0.056988099508522794
1107	2	0.023078943681269948
1107	3	0.0650899254384214
1107	4	0.030436822216572068
1107	5	0.026615131293951322
1107	6	0.08743550940134526
1107	7	0.045715293311246796
1107	8	0.05559808591445403
1107	9	0.06250435301013368
1107	10-11	0.08981002907323443
1107	12-13	0.06810649916786815
1107	14-15	0.15795617993112643
1107	16-17	0.22915061261531378
1107	18-19	0.15028814597457796
1107	20-21	0.12505870748761083
1107	22-23	0.12145292579459266
1107	24-25	0.11147129527538624
1107	26-27	0.15609331706323104
1107	28-29	0.16540422360638019
1107	30-31	0.18891273113749918
1107	32-33	0.07478708145850277
1107	34-35	0.08219997612415852
1107	36-37	0.06518538816231967
1107	38-39	0.11688168334026727
1107	40-41	0.04862649282716802
1107	42-43	0.07986651683987134
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2216	46-47	-0.536912565018568
2216	48-49	-0.48750519120222435
2216	50-51	-0.5377469818911393
2216	52-53	-0.5111748961658122
2216	54-55	-0.4663464698239608
2216	56-57	-0.4762103006153424
2216	58-59	-0.4887878055946686
2216	60-61	-0.5191955731806672
2216	62-63	-0.5774813340350278
2216	64-65	-0.4629102475169802
2216	66-67	-0.45079950880035113
2216	68-69	-0.4380568373523914
2216	70-71	-0.3978711994441184
2216	72-73	-0.3474964369288358
2216	74-75	-0.3614834333197905
2216	76-77	-0.2990862891669366
2216	78-79	-0.28487980277199654
2216	80-81	-0.36851053771342634
2216	82-83	-0.40331089795259345
2216	84-85	-0.342920356381633
2216	86-87	-0.24204418987460485
2216	88-89	-0.22466911750765917
2216	90-91	-0.286169968962966
2216	92-93	-0.2761772776397038
2216	94-95	-0.2837649202194541
2216	96-97	-0.30754659181398836
2216	98-99	-0.29945790403935035
2216	100	-0.4739632545688863
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	30.0
8	133.0
9	288.0
10	285.0
11	193.0
12	228.0
13	296.0
14	513.0
15	995.0
16	1775.0
17	3266.0
18	5539.0
19	8698.0
20	12410.0
21	18590.0
22	26343.0
23	30371.0
24	28547.0
25	24563.0
26	22921.0
27	25785.0
28	32084.0
29	38854.0
30	47213.0
31	59685.0
32	76514.0
33	100409.0
34	143444.0
35	220543.0
36	400109.0
37	906208.0
38	1215625.0
39	422594.0
40	880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.950829603377656	20.056875588906422	13.659120064151454	24.333174743564474
2	15.188534369233436	20.870211817352427	40.05227834723845	23.888975466175687
3	17.447860833220457	26.424195514619093	31.336803472719417	24.791140179441037
4	12.228477616101229	18.158616826579316	39.15619296900611	30.45671258831334
5	12.594169690305273	38.285005427198016	35.62708454328329	13.493740339213423
6	29.26625123072382	39.1883879530457	17.991324558126287	13.5540362581042
7	25.301140474084683	33.70342926552891	23.349919451631244	17.64551080875516
8	25.364184315370643	35.06543733336223	20.84306650133826	18.727311849928867
9	26.236631602412015	14.651340632394996	21.040926414601703	38.07110135059129
10-11	24.284203864415474	26.22728641314121	29.020887637518644	20.46762208492467
12-13	25.22114030350886	24.40519482663916	28.648392528973027	21.725272340878956
14-15	22.493890179976045	25.259573018879973	26.906802427544857	25.339734373599125
16-17	21.334679592092368	28.124294926645284	27.67374458726191	22.86728089400044
18-19	21.254200284422208	27.048696467013833	30.197284366479458	21.499818882084497
20-21	22.45869761187391	26.18351072750099	29.913677402509748	21.444114258115352
22-23	22.260446710508152	26.136842406718593	29.638051018941763	21.964659863831496
24-25	22.052058175412217	26.286767598923923	29.92930992357195	21.73186430209191
26-27	21.890485009259724	26.514928303584746	29.666613862080958	21.927972825074576
28-29	21.663222764686594	26.610534805426205	29.84258241822034	21.88366001166687
30-31	22.188598728949394	26.31660421924817	29.681514940617436	21.813282111185
32-33	22.09499247664043	26.584446357104675	29.253149181305744	22.067411984949153
34-35	22.24421838534947	26.573245610696894	29.635505402124934	21.5470306018287
36-37	22.333452874535915	26.336311543294126	29.358295944457204	21.971939637712758
38-39	22.203276089869167	26.365159997636678	29.57022122082066	21.861342691673496
40-41	22.25731355960409	26.0888197554282	29.659149047825327	21.994717637142386
42-43	22.302784903660616	26.15704399431053	29.181722181472175	22.358448920556675
44-45	22.233035433937314	26.643217592120784	28.972356735193245	22.151390238748665
46-47	22.493087470810256	26.498587565884137	28.386120193512117	22.62220476979349
48-49	22.793816865395293	26.513878409326487	28.245475505817247	22.44682921946098
50-51	22.54461054925356	26.73463395781093	27.95092175225939	22.769833740676116
52-53	22.39645637178013	27.013973089447664	27.931833893652712	22.657736645119495
54-55	22.371291003876763	26.889580109362594	27.80612521330453	22.933003673456117
56-57	22.672664711780747	26.827306843429426	27.74285497194931	22.757173472840513
58-59	22.305253865652055	26.793980802128846	28.17789217132492	22.722873160894178
60-61	22.467416064292465	26.910162844212447	27.886681150995905	22.73573994049918
62-63	22.408176423457167	26.735389195532576	27.791201815205635	23.06523256580462
64-65	22.012551355602135	26.787887043991667	27.921283639789923	23.27827796061628
66-67	22.342603206644977	26.649498992360204	27.826841790360866	23.181056010633945
68-69	22.108724274292985	26.556829859635624	28.036895734370944	23.297550131700447
70-71	22.157375277781863	26.33599111201243	27.97760355106684	23.529030059138865
72-73	22.333133461753405	26.33180780747929	27.924566711267957	23.410492019499348
74-75	22.170141500342766	26.67553532528979	27.781313946129092	23.373009228238352
76-77	22.157916654411025	26.507314941098098	27.63817526651506	23.69659313797582
78-79	22.021245744839497	26.640981330184978	27.576239421117567	23.761533503857958
80-81	21.9374775857932	26.6496082190155	27.716937271181585	23.695976924009713
82-83	22.11962382930416	26.291364586289635	28.08853427591011	23.50047730849609
84-85	22.25357974090723	26.46067025825426	27.594971914975304	23.69077808586321
86-87	21.724772038728744	26.70185819799493	27.736177036106795	23.837192727169526
88-89	22.04057706879866	26.208850875389228	27.661159261524904	24.089412794287206
90-91	21.877596955365327	26.19051980516691	27.745048740522606	24.186834498945156
92-93	22.035571605616564	26.27210486467952	27.628231532134816	24.064091997569097
94-95	21.51661665716357	26.197519047041613	27.853986011099273	24.431878284695543
96-97	21.556339399040045	26.171236189230644	27.757932287223497	24.51449212450581
98-99	21.380004282843085	26.355248933804276	27.54892374217285	24.715823041179792
100	21.680391380256758	26.00528987142473	27.430002411528655	24.88431633678986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	209.0
1	135.0
2	195.0
3	332.5
4	242.0
5	203.0
6	393.0
7	1098.5
8	2200.5
9	2727.0
10	2077.5
11	1414.0
12	1468.5
13	1724.0
14	2025.0
15	2413.0
16	2841.0
17	3312.5
18	4069.5
19	4896.5
20	5653.0
21	6682.0
22	8185.5
23	10110.5
24	12432.5
25	15509.0
26	19788.0
27	25034.0
28	30494.5
29	37392.0
30	45079.5
31	52618.5
32	62772.5
33	72679.0
34	80353.0
35	86832.0
36	94454.5
37	105245.0
38	110939.0
39	111374.5
40	113578.0
41	116430.5
42	119220.0
43	125421.5
44	133297.0
45	143442.0
46	152849.5
47	158960.0
48	162805.0
49	159867.0
50	152692.5
51	149440.0
52	147577.5
53	143253.0
54	137441.0
55	124468.5
56	111453.0
57	97792.5
58	83810.0
59	74552.5
60	60981.5
61	46930.0
62	36895.5
63	28098.5
64	20099.0
65	13598.5
66	9296.0
67	6617.0
68	4869.0
69	3492.5
70	2557.5
71	1898.5
72	1363.5
73	1047.5
74	861.0
75	588.5
76	349.5
77	203.0
78	116.5
79	79.5
80	48.5
81	24.5
82	17.0
83	14.0
84	9.5
85	9.0
86	6.0
87	3.0
88	2.0
89	0.5
90	0.5
91	2.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0027090251560075987
2	0.0036378337809244896
3	2.0640191664819798E-4
4	0.05012986550593109
5	0.002167220124806079
6	0.004257039530869084
7	2.5800239581024748E-5
8	2.8380263539127225E-4
9	2.5800239581024748E-5
10-11	1.0320095832409899E-4
12-13	0.0
14-15	1.2900119790512374E-5
16-17	1.6770155727666088E-4
18-19	1.2900119790512375E-4
20-21	1.8060167706717326E-4
22-23	0.0
24-25	0.0
26-27	1.8060167706717326E-4
28-29	0.0
30-31	1.4190131769563612E-4
32-33	1.2900119790512375E-4
34-35	1.1610107811461137E-4
36-37	7.740071874307425E-5
38-39	8.256076665927919E-4
40-41	4.1280383329639597E-4
42-43	0.0
44-45	4.1280383329639597E-4
46-47	0.0
48-49	6.450059895256187E-5
50-51	0.0
52-53	4.773044322489579E-4
54-55	4.902045520394703E-4
56-57	9.030083853358663E-5
58-59	8.901082655453538E-4
60-61	5.16004791620495E-4
62-63	2.1930203643871036E-4
64-65	4.386040728774207E-4
66-67	0.001135210541565089
68-69	0.002373622041454277
70-71	0.003612033541343465
72-73	0.002863826593493747
74-75	0.002889626833074772
76-77	0.004308640010031133
78-79	0.0013158122186322622
80-81	0.0011094103019840642
82-83	8.514079061738167E-4
84-85	6.19205749944594E-4
86-87	1.2900119790512374E-5
88-89	0.0
90-91	0.0
92-93	1.1610107811461137E-4
94-95	7.740071874307425E-5
96-97	4.5150419266793313E-4
98-99	7.740071874307425E-5
100	0.07407248783712206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3875933.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.58319090553428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.6184384067507	34.36391626163998
2	16.388253979976863	16.90716867315057
3	6.969369391171141	10.785069353879047
4	3.562076288590736	7.34973044857812
5	2.081051599673349	5.367364097510893
6	1.2321677556865849	3.81354867415349
7	0.8252005327325495	2.979653363070418
8	0.5411783005934532	2.233256287475577
9	0.3871820907161551	1.797487893055379
>10	1.363121054044308	10.547380487286548
>50	0.018766593566756758	0.6550955694390077
>100	0.01134724436603312	1.1237497313001485
>500	9.233810656842655E-4	0.34658005280446197
>1k	6.71549865950866E-4	0.554077755582182
>5k	8.394373324385826E-5	0.24655507504571303
>10k+	1.678874664877165E-4	0.9293662760284753
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11072	0.28566025264110606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10391	0.26809028948642816	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5694	0.14690656417435494	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.5800239581024748E-5	0.0
6	0.0	0.0	0.0	2.5800239581024748E-5	0.0
7	0.0	0.0	0.0	2.5800239581024748E-5	0.0
8	0.0	0.0	0.0	2.5800239581024748E-5	0.0
9	0.0	0.0	0.0	2.5800239581024748E-5	0.0
10-11	0.0	0.0	0.0	1.2900119790512375E-4	0.0
12-13	0.0	0.0	0.0	1.2900119790512375E-4	1.2900119790512374E-5
14-15	0.0	0.0	0.0	2.0640191664819798E-4	3.870035937153712E-5
16-17	0.0	0.0	0.0	2.0640191664819798E-4	5.1600479162049496E-5
18-19	0.0	0.0	0.0	2.1930203643871036E-4	5.1600479162049496E-5
20-21	0.0	0.0	0.0	2.7090251560075984E-4	5.1600479162049496E-5
22-23	0.0	0.0	0.0	4.1280383329639597E-4	5.1600479162049496E-5
24-25	0.0	0.0	0.0	6.19205749944594E-4	5.1600479162049496E-5
26-27	0.0	0.0	0.0	0.001173910900936626	5.1600479162049496E-5
28-29	0.0	0.0	0.0	0.0036120335413434646	5.1600479162049496E-5
30-31	0.0	0.0	0.0	0.012087412243710096	6.450059895256187E-5
32-33	0.0	0.0	0.0	0.03542372894474698	7.740071874307425E-5
34-35	0.0	0.0	0.0	0.07180206675399187	7.740071874307425E-5
36-37	0.0	0.0	0.0	0.13171022306113134	7.740071874307425E-5
38-39	0.0	0.0	0.0	0.2220497619540895	7.740071874307425E-5
40-41	0.0	0.0	0.0	0.3217934881743312	7.740071874307425E-5
42-43	0.0	0.0	0.0	0.4281549758471057	7.740071874307425E-5
44-45	0.0	0.0	0.0	0.5618002168768139	1.0320095832409899E-4
46-47	0.0	0.0	0.0	0.7261348428881511	1.548014374861485E-4
48-49	0.0	0.0	0.0	0.9025310809036173	1.8060167706717326E-4
50-51	0.0	0.0	0.0	1.0915565361939952	1.8060167706717326E-4
52-53	0.0	0.0	0.0	1.2813817989113847	1.8060167706717326E-4
54-55	0.0	0.0	0.0	1.493962872939238	1.8060167706717326E-4
56-57	0.0	0.0	0.0	1.749127242395573	1.8060167706717326E-4
58-59	0.0	0.0	0.0	2.034890695995003	1.8060167706717326E-4
60-61	0.0	0.0	0.0	2.332251357286104	1.8060167706717326E-4
62-63	0.0	0.0	0.0	2.6380228966806185	2.0640191664819798E-4
64-65	0.0	0.0	0.0	2.961403099589183	2.0640191664819798E-4
66-67	0.0	0.0	0.0	3.337131988607646	2.0640191664819798E-4
68-69	0.0	0.0	0.0	3.7492650156749354	2.0640191664819798E-4
70-71	0.0	0.0	0.0	4.194615851202794	2.0640191664819798E-4
72-73	0.0	0.0	0.0	4.652028298734782	2.1930203643871036E-4
74-75	0.0	0.0	0.0	5.111349963995766	2.580023958102475E-4
76-77	0.0	0.0	0.0	5.621356199913672	2.580023958102475E-4
78-79	0.0	0.0	0.0	6.175416344916179	2.580023958102475E-4
80-81	0.0	0.0	0.0	6.775762119727044	2.580023958102475E-4
82-83	0.0	0.0	0.0	7.392349145354164	2.580023958102475E-4
84-85	0.0	0.0	0.0	8.009387675173953	2.7090251560075984E-4
86-87	0.0	0.0	0.0	8.655554675480715	2.9670275518178465E-4
88	0.0	0.0	0.0	9.166180117148567	3.09602874972297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7285	0.0	59.45396	1
GTATCAA	13240	0.0	43.439938	1
ATCAACG	16145	0.0	34.7763	3
CAACGCA	16295	0.0	34.41044	5
TATCAAC	17055	0.0	33.612213	2
AACGCAG	17510	0.0	32.344006	6
TCAACGC	17605	0.0	31.918978	4
ACGCAGA	19160	0.0	29.288054	7
CGCAGAG	19215	0.0	29.033007	8
GCAGAGT	21720	0.0	25.44624	9
GTGGTAT	1925	0.0	24.9184	1
GTACATG	11450	0.0	24.848524	1
TACATGG	11705	0.0	24.387224	2
GAGTACT	15320	0.0	23.314909	12-13
ACATGGG	11985	0.0	23.266644	3
CAGAGTA	21110	0.0	22.730742	10-11
CATGGGG	9380	0.0	22.509226	4
AGAGTAC	20365	0.0	21.843	10-11
GTACTTT	16790	0.0	21.063839	14-15
TGATCGC	945	0.0	20.390512	9
>>END_MODULE