FastQCFastQC Report
Mon 16 Jan 2017
ERR1146608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062895
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.037.3860551
GTATCAA68250.036.72731
CAACGCA69000.034.068435
ATCAACG73050.032.3739853
AACGCAG80200.030.4702196
TCAACGC79350.029.8629044
TATCAAC85900.028.2973542
ACGCAGA88950.026.3108167
GTACATG147100.025.2568281
CGCAGAG95350.024.544568
TACATGG151750.023.6088432
ACATGGG149400.023.1305083
CATGGGG122350.019.7902684
GCAGAGT115000.019.4514269
CTAACGC6650.018.3823133
GTACTAG20250.018.3470611
GAGTACA110550.017.3141061
GTATAAG22850.017.2885071
ATGGGGG80300.017.2132575
AGAGTAC94600.017.08931510-11
Produced by FastQC (version 0.11.5)