##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146608_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5062895
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077777832643182	31.0	31.0	34.0	28.0	34.0
2	31.363544572818515	31.0	31.0	34.0	28.0	34.0
3	31.46237794779469	33.0	31.0	34.0	28.0	34.0
4	34.99537557069621	37.0	35.0	37.0	32.0	37.0
5	34.96006790581278	37.0	35.0	37.0	32.0	37.0
6	35.09015434845084	37.0	35.0	37.0	32.0	37.0
7	35.011694494948046	37.0	35.0	37.0	32.0	37.0
8	34.99060162219442	37.0	35.0	37.0	32.0	37.0
9	36.610107458282265	39.0	37.0	39.0	32.0	39.0
10-11	36.58829138269705	39.0	37.0	39.0	32.0	39.0
12-13	36.53093605535963	39.0	37.0	39.0	32.0	39.0
14-15	37.864209409833705	40.0	38.0	41.0	32.0	41.0
16-17	37.87692249197347	40.0	38.0	41.0	32.0	41.0
18-19	37.88720968931807	40.0	38.0	41.0	32.0	41.0
20-21	37.976416161109405	40.0	38.0	41.0	32.0	41.0
22-23	37.97811341139803	40.0	38.0	41.0	32.0	41.0
24-25	37.98855190557971	40.0	38.0	41.0	32.0	41.0
26-27	38.04144535883126	40.0	38.0	41.0	32.0	41.0
28-29	38.00966897397635	40.0	38.0	41.0	32.0	41.0
30-31	37.97006752065765	40.0	38.0	41.0	32.0	41.0
32-33	37.939230321782304	40.0	38.0	41.0	32.0	41.0
34-35	37.89127969274496	40.0	38.0	41.0	31.5	41.0
36-37	37.82067186856531	40.0	38.0	41.0	31.0	41.0
38-39	37.77087792656178	40.0	38.0	41.0	31.0	41.0
40-41	37.7215709589079	40.0	38.0	41.0	31.0	41.0
42-43	37.57769764136922	40.0	37.0	41.0	31.0	41.0
44-45	37.46717747454767	40.0	37.0	41.0	30.5	41.0
46-47	37.35998139404431	40.0	37.0	41.0	30.0	41.0
48-49	37.26050115595919	40.0	36.5	41.0	30.0	41.0
50-51	37.13743194358169	40.0	36.0	41.0	30.0	41.0
52-53	37.01790586215989	40.0	36.0	41.0	30.0	41.0
54-55	36.89696497754743	39.0	35.5	41.0	30.0	41.0
56-57	36.71386420220052	39.0	35.0	41.0	30.0	41.0
58-59	36.527286364816966	39.0	35.0	41.0	30.0	41.0
60-61	36.31280769204181	39.0	35.0	40.5	29.0	41.0
62-63	36.07921811532729	38.0	35.0	40.0	29.0	41.0
64-65	35.78582372338356	37.5	35.0	40.0	29.0	41.0
66-67	35.5380143178952	37.0	34.5	40.0	29.0	41.0
68-69	35.28021951867459	36.5	34.0	39.5	28.5	41.0
70-71	34.98946679715854	36.0	34.0	39.0	28.0	41.0
72-73	34.646538294789835	35.5	34.0	39.0	28.0	41.0
74-75	34.314442329931786	35.0	34.0	38.5	28.0	40.0
76-77	34.03564452748872	35.0	34.0	37.0	28.0	39.0
78-79	33.72607322490393	35.0	33.5	37.0	27.0	39.0
80-81	33.47523442615342	35.0	33.0	36.0	27.0	39.0
82-83	33.21034635322281	35.0	33.0	36.0	27.0	37.5
84-85	33.00560973514165	35.0	33.0	36.0	26.5	37.0
86-87	32.79721937745104	35.0	33.0	35.0	26.0	37.0
88-89	32.61655219000197	35.0	33.0	35.0	26.0	36.0
90-91	32.46795262394342	35.0	33.0	35.0	26.0	36.0
92-93	32.31956805345558	35.0	33.0	35.0	25.5	36.0
94-95	32.200544352588786	35.0	33.0	35.0	25.0	36.0
96-97	32.11131990689122	35.0	33.0	35.0	25.0	35.0
98-99	32.00075915064404	35.0	33.0	35.0	25.0	35.0
100	30.85961174387381	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2943278170094885
1101	2	0.3365464601131549
1101	3	0.31677995409446424
1101	4	0.37171332048512085
1101	5	0.31266712379250805
1101	6	0.40688376144200333
1101	7	0.38244186406492275
1101	8	0.42189417577365873
1101	9	0.5769219701426849
1101	10-11	0.5992139760841582
1101	12-13	0.5971467711485232
1101	14-15	0.7302487794619452
1101	16-17	0.6943947489994358
1101	18-19	0.6638091877863346
1101	20-21	0.6696959132087486
1101	22-23	0.6551068322336562
1101	24-25	0.6771058098694738
1101	26-27	0.6776164889351435
1101	28-29	0.6478421304808819
1101	30-31	0.6548200889809834
1101	32-33	0.6678937229260455
1101	34-35	0.6360413892201535
1101	36-37	0.6539751737278152
1101	38-39	0.5902508312129626
1101	40-41	0.5713034452304342
1101	42-43	0.601059389469988
1101	44-45	0.5802083262429392
1101	46-47	0.5200236017494078
1101	48-49	0.518702906555923
1101	50-51	0.5279589444368398
1101	52-53	0.5266440340876599
1101	54-55	0.5638223328102754
1101	56-57	0.6500740495546751
1101	58-59	0.5769474256306992
1101	60-61	0.5584254848058166
1101	62-63	0.48989185938555124
1101	64-65	0.5628386630458309
1101	66-67	0.5058046123766502
1101	68-69	0.5434454055924647
1101	70-71	0.5172681313206482
1101	72-73	0.5800332243170416
1101	74-75	0.5360402707477121
1101	76-77	0.498103363861631
1101	78-79	0.5561120590765967
1101	80-81	0.5094782901351991
1101	82-83	0.5685663883088736
1101	84-85	0.46209219208507335
1101	86-87	0.44906144609987564
1101	88-89	0.42582958009471383
1101	90-91	0.4459422285050607
1101	92-93	0.44615734632321136
1101	94-95	0.4366293491509694
1101	96-97	0.4269744867246601
1101	98-99	0.4617784160140914
1101	100	0.4280131034792767
1102	1	0.24005594970842026
1102	2	0.27117202400817675
1102	3	0.29336519396868255
1102	4	0.3268955069507413
1102	5	0.3530596863375095
1102	6	0.3773247049104427
1102	7	0.40061331360607255
1102	8	0.4142904838530015
1102	9	0.4649347090958287
1102	10-11	0.4598405443742024
1102	12-13	0.4406263701700581
1102	14-15	0.535533659220242
1102	16-17	0.5240730641824669
1102	18-19	0.5096979560753496
1102	20-21	0.5619304322876886
1102	22-23	0.5090231707418198
1102	24-25	0.6227926451753376
1102	26-27	0.5613040054159839
1102	28-29	0.5536564653562053
1102	30-31	0.5991147447536207
1102	32-33	0.5696016187091857
1102	34-35	0.5612853520782153
1102	36-37	0.5315352309422678
1102	38-39	0.5283267160676033
1102	40-41	0.46854207482517296
1102	42-43	0.5335599936846549
1102	44-45	0.4718656098502265
1102	46-47	0.4837140123621637
1102	48-49	0.5053467463040135
1102	50-51	0.47502267990336833
1102	52-53	0.440904116716915
1102	54-55	0.37426173044371325
1102	56-57	0.4654496456987829
1102	58-59	0.40022762784607124
1102	60-61	0.471670864635108
1102	62-63	0.48087427862793675
1102	64-65	0.5055221901852462
1102	66-67	0.4375899033191075
1102	68-69	0.46871038173079427
1102	70-71	0.3686461384900852
1102	72-73	0.3376300885788055
1102	74-75	0.42446435957675277
1102	76-77	0.35339666881841936
1102	78-79	0.30760744894851655
1102	80-81	0.2706605039114507
1102	82-83	0.2975504233863333
1102	84-85	0.349470190769658
1102	86-87	0.230913071394248
1102	88-89	0.2929673485957096
1102	90-91	0.29371877818807945
1102	92-93	0.26906093354867977
1102	94-95	0.30113893299654393
1102	96-97	0.2470452583919709
1102	98-99	0.3060545310470246
1102	100	0.2971308599132172
1103	1	0.1292851850302803
1103	2	0.18932309579459528
1103	3	0.2284767260698679
1103	4	0.2238156809023195
1103	5	0.17056412152155787
1103	6	0.3123847375069033
1103	7	0.2876121144589163
1103	8	0.32361827651418196
1103	9	0.3986026984046873
1103	10-11	0.3671422142034828
1103	12-13	0.40567857276625574
1103	14-15	0.40676336361568133
1103	16-17	0.4236396251519139
1103	18-19	0.44597737241083024
1103	20-21	0.3910087001193503
1103	22-23	0.3666765290024756
1103	24-25	0.4071289994670195
1103	26-27	0.358448558947714
1103	28-29	0.3795697837767378
1103	30-31	0.42619529880008145
1103	32-33	0.46239404898249603
1103	34-35	0.43050501912937733
1103	36-37	0.40400351788454714
1103	38-39	0.41677055151202325
1103	40-41	0.3740338029928836
1103	42-43	0.4087945953327434
1103	44-45	0.32076067954449883
1103	46-47	0.325340906363472
1103	48-49	0.32665565875380764
1103	50-51	0.35354702321334486
1103	52-53	0.3727435493039337
1103	54-55	0.3087502742037671
1103	56-57	0.34702569139817285
1103	58-59	0.33771241797975193
1103	60-61	0.2894825456048906
1103	62-63	0.33670777151893105
1103	64-65	0.2564067622827366
1103	66-67	0.26949947641544725
1103	68-69	0.23807568730575923
1103	70-71	0.25402095370156275
1103	72-73	0.29822207084534114
1103	74-75	0.2758878344260509
1103	76-77	0.2501730462939591
1103	78-79	0.30103977029712325
1103	80-81	0.19903772987051838
1103	82-83	0.21490052869439324
1103	84-85	0.23031804384999788
1103	86-87	0.2250000980333695
1103	88-89	0.19207469483791328
1103	90-91	0.26161856611908263
1103	92-93	0.29466111681946217
1103	94-95	0.25565351967993877
1103	96-97	0.2778792571974975
1103	98-99	0.27321516487750586
1103	100	0.22497240021800025
1104	1	0.09405459124263871
1104	2	0.17372843599983057
1104	3	0.12433630625193359
1104	4	0.1992767697433777
1104	5	0.1311714686645189
1104	6	0.2007488265520152
1104	7	0.15369805931256053
1104	8	0.24391490704412178
1104	9	0.32281397648635135
1104	10-11	0.31855180985391485
1104	12-13	0.30926517584807556
1104	14-15	0.4056692238211852
1104	16-17	0.39257911258962963
1104	18-19	0.36361463767020297
1104	20-21	0.3212741141777897
1104	22-23	0.34101122486814006
1104	24-25	0.4125274167573565
1104	26-27	0.38979038177900804
1104	28-29	0.3902729442593511
1104	30-31	0.39859686077556233
1104	32-33	0.36604721538915186
1104	34-35	0.34979719345453475
1104	36-37	0.40518758810432587
1104	38-39	0.36156355520468964
1104	40-41	0.3584956274039328
1104	42-43	0.40753326558017733
1104	44-45	0.4208185213459359
1104	46-47	0.4041453418340737
1104	48-49	0.3855887613781661
1104	50-51	0.39814798883408287
1104	52-53	0.327019070456096
1104	54-55	0.3601854996140048
1104	56-57	0.4248150978738252
1104	58-59	0.34068374226902876
1104	60-61	0.3673150660264213
1104	62-63	0.3514640543069234
1104	64-65	0.40207023160601096
1104	66-67	0.33069067195529556
1104	68-69	0.32701589056662783
1104	70-71	0.29398523659487097
1104	72-73	0.34652009846459464
1104	74-75	0.33783759680567726
1104	76-77	0.3028125824405592
1104	78-79	0.31098847786608985
1104	80-81	0.3253422969867543
1104	82-83	0.2932319037988833
1104	84-85	0.26045251163960614
1104	86-87	0.23808814263490774
1104	88-89	0.22462391385017355
1104	90-91	0.30790842183547795
1104	92-93	0.2698701454063084
1104	94-95	0.3141181695354547
1104	96-97	0.2844983175109448
1104	98-99	0.33101393464902884
1104	100	0.38876762872191506
1105	1	0.12688739244032732
1105	2	0.15657307657912156
1105	3	0.18357350635275793
1105	4	0.22443641043724227
1105	5	0.1887463813968182
1105	6	0.19484298018899437
1105	7	0.23560729326637642
1105	8	0.29278882908752024
1105	9	0.3623323046128988
1105	10-11	0.33804363304228957
1105	12-13	0.34017369561984623
1105	14-15	0.4263612165800623
1105	16-17	0.3836060548210867
1105	18-19	0.33583691824063777
1105	20-21	0.3611671969236099
1105	22-23	0.3743495867560256
1105	24-25	0.4067318962446791
1105	26-27	0.36140761266223365
1105	28-29	0.3588313181393801
1105	30-31	0.37972078090938055
1105	32-33	0.378177998545155
1105	34-35	0.344132813188871
1105	36-37	0.3711807785503254
1105	38-39	0.42774789437623895
1105	40-41	0.3440355891359914
1105	42-43	0.37504772108319884
1105	44-45	0.3262515562759347
1105	46-47	0.31543624498792866
1105	48-49	0.34338160466294454
1105	50-51	0.3140596403132392
1105	52-53	0.2901321403695505
1105	54-55	0.2893688591722068
1105	56-57	0.35439674759616224
1105	58-59	0.3235058498317187
1105	60-61	0.34195071860549575
1105	62-63	0.3111576912121805
1105	64-65	0.3888345190661937
1105	66-67	0.3238221120060132
1105	68-69	0.31835910660097255
1105	70-71	0.3000296705578549
1105	72-73	0.266220399795948
1105	74-75	0.27404972988032483
1105	76-77	0.2792391801842058
1105	78-79	0.24078485959830687
1105	80-81	0.24633108585620533
1105	82-83	0.2196133877698898
1105	84-85	0.19544881660679891
1105	86-87	0.1602917570835345
1105	88-89	0.14448428553036763
1105	90-91	0.14250085112381328
1105	92-93	0.24815972713241052
1105	94-95	0.2618541742406606
1105	96-97	0.21657989085018414
1105	98-99	0.14383684423435028
1105	100	0.2326710155488847
1106	1	0.10056651800199745
1106	2	0.1511748871273504
1106	3	0.13753647641441802
1106	4	0.14485319900824578
1106	5	0.20091477870948893
1106	6	0.21236220461582178
1106	7	0.23014636361108387
1106	8	0.2473192721888111
1106	9	0.2755680393849289
1106	10-11	0.2866190348053337
1106	12-13	0.2774454257684553
1106	14-15	0.25542971166132844
1106	16-17	0.22744766168519703
1106	18-19	0.31449909603501425
1106	20-21	0.27250004180181264
1106	22-23	0.3228483412140406
1106	24-25	0.360931268414447
1106	26-27	0.3595482406395405
1106	28-29	0.2990371488266632
1106	30-31	0.30195220420068836
1106	32-33	0.3195647785885214
1106	34-35	0.3139640280784448
1106	36-37	0.3134841054659745
1106	38-39	0.3026325440918143
1106	40-41	0.2712372839034103
1106	42-43	0.31265479482704706
1106	44-45	0.25480407368935687
1106	46-47	0.3454741227567908
1106	48-49	0.31052992185246353
1106	50-51	0.316493131741332
1106	52-53	0.2796657432871825
1106	54-55	0.2791367790635775
1106	56-57	0.24025111231762963
1106	58-59	0.25434595511983815
1106	60-61	0.2917780387619544
1106	62-63	0.2794371379403984
1106	64-65	0.2994503197489422
1106	66-67	0.2924348451210079
1106	68-69	0.24967359857334515
1106	70-71	0.20538486342207563
1106	72-73	0.2704437649738125
1106	74-75	0.26756234629296216
1106	76-77	0.22660462688281058
1106	78-79	0.207133686007424
1106	80-81	0.22869951550708123
1106	82-83	0.21731472261891582
1106	84-85	0.17077400680782517
1106	86-87	0.20508088995900664
1106	88-89	0.1890945715912551
1106	90-91	0.13342734241143717
1106	92-93	0.15674323857873418
1106	94-95	0.17311985731345203
1106	96-97	0.1921522253247261
1106	98-99	0.16644629121483234
1106	100	0.2202035131591238
1107	1	0.1370619981934773
1107	2	0.17913162398033577
1107	3	0.1838920545689433
1107	4	0.15669477012221478
1107	5	0.17653216477057043
1107	6	0.23197373958385725
1107	7	0.2607196292193166
1107	8	0.18328006205339875
1107	9	0.35298989720627105
1107	10-11	0.3125820510195467
1107	12-13	0.3334412333718362
1107	14-15	0.35607324615478575
1107	16-17	0.32933212227414543
1107	18-19	0.3372708218051699
1107	20-21	0.3599573922383641
1107	22-23	0.3454288614389611
1107	24-25	0.3922106745228575
1107	26-27	0.3253554530523104
1107	28-29	0.30239603840144014
1107	30-31	0.36359262887651767
1107	32-33	0.3359166955044941
1107	34-35	0.3150869770639275
1107	36-37	0.3127209321614757
1107	38-39	0.3288380092671659
1107	40-41	0.3836533840313976
1107	42-43	0.35047666787667
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2216	64-65	-0.5681765033874626
2216	66-67	-0.5125969943053832
2216	68-69	-0.659946480910115
2216	70-71	-0.5989556415518749
2216	72-73	-0.5183192395347334
2216	74-75	-0.5001387948407015
2216	76-77	-0.5348769616453808
2216	78-79	-0.5191138654256946
2216	80-81	-0.4826572498761976
2216	82-83	-0.47576299329608673
2216	84-85	-0.44722387620422666
2216	86-87	-0.5223871586594484
2216	88-89	-0.5133935064316972
2216	90-91	-0.49639980094548264
2216	92-93	-0.48787795792852506
2216	94-95	-0.5476761840255158
2216	96-97	-0.5259337009855756
2216	98-99	-0.47246647761637917
2216	100	-0.5576301622277917
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1536.0
1	397.0
2	274.0
3	261.0
4	217.0
5	275.0
6	374.0
7	740.0
8	1997.0
9	2871.0
10	3391.0
11	4810.0
12	6916.0
13	9665.0
14	11557.0
15	13189.0
16	14496.0
17	15862.0
18	17895.0
19	19808.0
20	21633.0
21	24246.0
22	27274.0
23	31004.0
24	37387.0
25	44790.0
26	50200.0
27	49825.0
28	54036.0
29	65706.0
30	76746.0
31	87994.0
32	110933.0
33	147542.0
34	212024.0
35	320871.0
36	583309.0
37	1170529.0
38	1403102.0
39	416506.0
40	707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.368295667749415	19.777169733358903	13.398186279064712	24.456348319826972
2	14.948974297908673	20.429649554667712	40.44893921681204	24.172436930611568
3	18.051156576507445	25.861885268828104	30.13778185504905	25.949176299615395
4	12.391000231080596	17.512647659809826	39.36251406201539	30.733838047094185
5	13.368902137686458	38.185477016015845	35.34684526186362	13.098775584434069
6	30.304248776117465	38.127410303307784	18.537955580437806	13.030385340136942
7	26.615988673559478	32.538311938111015	23.26743770912382	17.578261679205696
8	25.214720324392903	35.663806891965436	20.946645456401892	18.174827327239765
9	26.168723984569276	15.746205421072698	20.64222078459347	37.44284980976455
10-11	24.489603089032354	26.385161843990545	29.02794375214287	20.09729131483423
12-13	24.953590968027576	24.195431833275354	28.33563929034502	22.515337908352045
14-15	22.475643852954526	25.470125798129388	25.72411188631765	26.330118462598428
16-17	22.492143249876232	28.202809858690674	26.488977323279826	22.81606956815326
18-19	22.396203041338506	28.12645103442632	27.539276888946606	21.938069035288564
20-21	23.501360411842438	27.350410287877992	26.934009920515894	22.214219379763676
22-23	23.4350129938927	27.090954283932493	26.647308838564904	22.826723883609905
24-25	23.00320044562316	27.3371248727264	26.94206523326922	22.717609448381225
26-27	23.008159593315977	27.51275161908576	26.667716886236022	22.811371901362246
28-29	22.7677043862231	27.679485144451753	26.752697320341888	22.80011314898325
30-31	23.159237101669532	27.331711088942175	26.712146046671336	22.796905762716957
32-33	23.08636329086436	27.594666105768734	26.3822053548686	22.93676524849831
34-35	23.277431739117326	27.557820416066804	26.68745369745727	22.4772941473586
36-37	23.185104952462552	27.362851301974345	26.682265041367554	22.76977870419555
38-39	23.095148827548563	27.39791171219811	26.81115963699262	22.695779823260708
40-41	23.073531238907954	27.110282279008885	26.988705301062655	22.82748118102051
42-43	22.985168311419496	27.13363248114593	26.793713052370304	23.08748615506427
44-45	22.977713379336333	27.38573375953864	26.855925993762696	22.78062686736233
46-47	22.951252308455064	27.290475702855627	26.663253981652552	23.09501800703676
48-49	23.057131698340488	27.131084479265283	26.866204844007584	22.94557897838665
50-51	22.99724728999219	27.204303115994975	26.73184089162183	23.066608702391004
52-53	22.924997694257907	27.277655535278644	26.68989642102366	23.107450349439787
54-55	22.879207641285333	27.174277481330122	26.737727778814552	23.20878709856999
56-57	23.077096446995558	27.132295440214254	26.68472615060261	23.105881962187578
58-59	22.83989823824844	27.08788294729475	26.98037769683717	23.09184111761964
60-61	22.88936073335161	27.174227551852503	26.823154973424394	23.113256741371494
62-63	22.881045794535908	27.05683351338185	26.756471465605465	23.30564922647678
64-65	22.692794906557143	27.080881261149862	26.77741910163654	23.44890473065645
66-67	22.856189008274406	26.989487029145337	26.689301440999568	23.46502252158069
68-69	22.69935575456394	26.860427987753706	26.861792202425733	23.578424055256626
70-71	22.686532814644142	26.73080191100805	26.891110364402195	23.69155490994562
72-73	22.783175628826086	26.814214738713492	26.89020511965791	23.512404512802508
74-75	22.670943462033293	26.930326368942602	26.873838029721526	23.52489213930258
76-77	22.679843078824778	26.81496951457009	26.811212959876435	23.693974446728696
78-79	22.59047954619198	26.814736778416236	26.829111557579495	23.76567211781229
80-81	22.530021456325343	26.81006604562217	26.993172412211365	23.666740085841116
82-83	22.680688066206525	26.587573028809153	27.277428654332198	23.454310250652124
84-85	22.800598809676455	26.67293170087869	26.89184541472948	23.634624074715376
86-87	22.359018514802255	26.81183389368397	27.07363511535051	23.75551247616327
88-89	22.7101100745459	26.40377445893698	26.97778846734622	23.9083269991709
90-91	22.648148792539494	26.448746986884647	27.05058909964001	23.852515120935852
92-93	22.687177792027562	26.460957040650413	27.050044131612538	23.801821035709487
94-95	22.32477312795035	26.344440232355726	27.25355944836026	24.077227191333662
96-97	22.43253766595295	26.28905044898446	27.16277185670099	24.115640028361597
98-99	22.33353995327819	26.4356378871776	27.020935840058367	24.209886319485843
100	22.625136620677637	26.165750582122886	26.797037113304086	24.41207568389539
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1701.0
1	1092.0
2	499.0
3	517.0
4	483.5
5	441.5
6	453.0
7	456.5
8	459.5
9	484.5
10	475.0
11	463.0
12	465.0
13	467.5
14	527.0
15	613.0
16	717.5
17	867.0
18	1096.0
19	1484.5
20	2118.0
21	3026.0
22	4320.0
23	6341.5
24	9339.5
25	13719.5
26	19992.0
27	27302.0
28	35046.0
29	44578.5
30	55016.5
31	64523.5
32	76009.0
33	88062.5
34	97653.0
35	106696.5
36	118907.5
37	134949.5
38	144155.5
39	148736.5
40	153318.0
41	155200.5
42	158483.5
43	165855.0
44	175036.0
45	186918.5
46	195694.5
47	200211.5
48	205535.0
49	204794.5
50	200111.0
51	199479.5
52	197820.5
53	192836.0
54	185397.5
55	171807.0
56	157613.5
57	139879.5
58	120291.5
59	105092.0
60	88056.0
61	70361.5
62	55890.0
63	43668.0
64	32523.0
65	23172.0
66	16538.0
67	11793.0
68	8517.0
69	6286.0
70	5069.0
71	4008.5
72	2936.0
73	2273.0
74	1831.0
75	1376.0
76	1031.5
77	758.5
78	549.5
79	376.0
80	256.0
81	201.0
82	145.5
83	104.0
84	80.0
85	59.0
86	44.0
87	37.5
88	32.0
89	24.0
90	20.5
91	12.5
92	8.5
93	10.0
94	9.0
95	9.0
96	8.0
97	7.5
98	11.5
99	9.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0945111443156534
2	0.06300742954376892
3	0.07813711325239808
4	0.08007276469292766
5	0.08206767076939182
6	0.08696605400665035
7	0.08471437784113635
8	0.08568220356140113
9	0.07185612184333272
10-11	0.07830500138754605
12-13	0.0698217126762455
14-15	0.07814698902505385
16-17	0.07956910028748373
18-19	0.07908518742735135
20-21	0.07074015953323148
22-23	0.0748089778674059
24-25	0.07726804525869092
26-27	0.07180674298005389
28-29	0.08054680178040428
30-31	0.08425021652631548
32-33	0.08571183087936843
34-35	0.0879437554995709
36-37	0.0881313951800304
38-39	0.08968189148698522
40-41	0.08634388032933726
42-43	0.09159779138220327
44-45	0.088022761680817
46-47	0.08526742110985909
48-49	0.09379021291178269
50-51	0.09099536925020171
52-53	0.0961999014397889
54-55	0.08846717145032634
56-57	0.09471853554142441
58-59	0.09880710542091037
60-61	0.09809604978969542
62-63	0.09873797501232004
64-65	0.10069337799816112
66-67	0.10252039593947732
68-69	0.09954778837009261
70-71	0.10163157640045863
72-73	0.09911325437323903
74-75	0.1029845572542982
76-77	0.10012058318412687
78-79	0.10717188486034175
80-81	0.10229325316839476
82-83	0.10641145036584801
84-85	0.10259940212072342
86-87	0.09974530382320787
88-89	0.09884660851153342
90-91	0.09889598737481224
92-93	0.09341493355086368
94-95	0.09288164182745248
96-97	0.091775555290007
98-99	0.10468319015108944
100	0.24521543504259913
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5062895.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.569856018698445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46078357121432	37.35895102408441
2	15.74467073038108	17.183688296374225
3	6.54801672670327	10.719729899526795
4	3.349010662212971	7.31020118568191
5	1.8344632814714847	5.0053198570744
6	1.1612934173373424	3.8022968747736634
7	0.7559990721496738	2.8878332362234116
8	0.5011489750171302	2.187810192848248
9	0.34306943668762485	1.6849124788013703
>10	1.2759419303959045	10.680108060655089
>50	0.020623595388243956	0.7511615743717384
>100	0.004913631140339044	0.3951923869501504
>500	6.496990062518137E-5	0.03279493263465397
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.950309062305262E-5
2	0.0	1.975154531152631E-5	0.0	9.875772655763155E-5	3.950309062305262E-5
3	0.0	1.975154531152631E-5	0.0	9.875772655763155E-5	3.950309062305262E-5
4	0.0	1.975154531152631E-5	0.0	1.1850927186915787E-4	3.950309062305262E-5
5	0.0	1.975154531152631E-5	0.0	1.3826081718068417E-4	3.950309062305262E-5
6	0.0	1.975154531152631E-5	0.0	1.3826081718068417E-4	2.172669984267894E-4
7	0.0	1.975154531152631E-5	0.0	1.3826081718068417E-4	2.172669984267894E-4
8	0.0	1.975154531152631E-5	0.0	1.3826081718068417E-4	2.56770089049842E-4
9	0.0	1.975154531152631E-5	0.0	1.5801236249221047E-4	2.7652163436136834E-4
10-11	0.0	1.975154531152631E-5	0.0	1.975154531152631E-4	2.7652163436136834E-4
12-13	9.875772655763155E-6	1.975154531152631E-5	0.0	1.975154531152631E-4	2.7652163436136834E-4
14-15	1.975154531152631E-5	1.975154531152631E-5	0.0	1.975154531152631E-4	3.851551335747631E-4
16-17	1.975154531152631E-5	1.975154531152631E-5	0.0	1.975154531152631E-4	4.5428554216510515E-4
18-19	1.975154531152631E-5	2.9627317967289464E-5	0.0	1.975154531152631E-4	4.937886327881578E-4
20-21	1.975154531152631E-5	3.950309062305262E-5	0.0	2.468943163940789E-4	5.135401780996841E-4
22-23	1.975154531152631E-5	3.950309062305262E-5	0.0	3.7527936091899994E-4	6.122979046573156E-4
24-25	1.975154531152631E-5	6.913040859034209E-5	0.0	5.135401780996841E-4	6.913040859034209E-4
26-27	1.975154531152631E-5	7.900618124610524E-5	0.0	8.394406757398682E-4	6.913040859034209E-4
28-29	1.975154531152631E-5	7.900618124610524E-5	0.0	0.0023109308014485786	6.913040859034209E-4
30-31	1.975154531152631E-5	9.875772655763155E-5	0.0	0.007456208355101182	7.209314038707103E-4
32-33	1.975154531152631E-5	9.875772655763155E-5	0.0	0.02347471160274902	7.900618124610524E-4
34-35	1.975154531152631E-5	9.875772655763155E-5	0.0	0.048470292194485565	8.690679937071576E-4
36-37	1.975154531152631E-5	9.875772655763155E-5	0.0	0.09179530683531853	8.690679937071576E-4
38-39	1.975154531152631E-5	9.875772655763155E-5	0.0	0.1617651561014005	8.986953116744471E-4
40-41	1.975154531152631E-5	9.875772655763155E-5	0.0	0.2402380456240945	9.48074174953263E-4
42-43	1.975154531152631E-5	9.875772655763155E-5	0.0	0.3225526107098804	9.777014929205523E-4
44-45	1.975154531152631E-5	9.875772655763155E-5	0.0	0.42437182679079855	9.875772655763156E-4
46-47	1.975154531152631E-5	9.875772655763155E-5	0.0	0.5522927100008987	0.0010369561288551313
48-49	1.975154531152631E-5	9.875772655763155E-5	0.0	0.6916201106284052	0.0012443473546261575
50-51	1.975154531152631E-5	9.875772655763155E-5	0.0	0.8392332845140972	0.0012443473546261575
52-53	1.975154531152631E-5	9.875772655763155E-5	0.0	0.988683352113761	0.0013233535358722628
54-55	1.975154531152631E-5	1.086334992133947E-4	0.0	1.1588824180631832	0.001402359717118368
56-57	1.975154531152631E-5	1.1850927186915787E-4	0.0	1.360367931786063	0.001402359717118368
58-59	1.975154531152631E-5	1.1850927186915787E-4	0.0	1.5920733098355782	0.0014122354897741314
60-61	1.975154531152631E-5	1.1850927186915787E-4	0.0	1.839856445768676	0.0014616143530529469
62-63	1.975154531152631E-5	1.1850927186915787E-4	0.0	2.091362747992996	0.0015603720796105785
64-65	1.975154531152631E-5	1.2838504452492103E-4	0.0	2.3569519020244347	0.0015899993975778681
66-67	1.975154531152631E-5	1.777639078037368E-4	0.0	2.6596344581509195	0.0016393782608566838
68-69	1.975154531152631E-5	2.172669984267894E-4	0.0	2.9982549509717265	0.0016591298061682102
70-71	1.975154531152631E-5	2.172669984267894E-4	0.0	3.365100402042705	0.0017282602147585522
72-73	1.975154531152631E-5	2.172669984267894E-4	0.0	3.7440337988443373	0.0019949060764641573
74-75	1.975154531152631E-5	2.172669984267894E-4	0.0	4.125248499129451	0.0019949060764641573
76-77	1.975154531152631E-5	2.172669984267894E-4	0.0	4.552227529901371	0.002004781849119921
78-79	1.975154531152631E-5	2.172669984267894E-4	0.0	5.017919589483882	0.002014657621775684
80-81	1.975154531152631E-5	2.2714277108255257E-4	0.0	5.524665235996402	0.0020541607123987365
82-83	1.975154531152631E-5	2.3701854373831574E-4	0.0	6.044891312184037	0.0021924215295794206
84-85	1.975154531152631E-5	2.3701854373831574E-4	0.0	6.561986768439796	0.0021924215295794206
86-87	1.975154531152631E-5	2.3701854373831574E-4	0.0	7.107168922128545	0.0022121730748909467
88	1.975154531152631E-5	2.3701854373831574E-4	0.0	7.537169939333128	0.0022516761655139994
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4365	0.0	37.386055	1
GTATCAA	6825	0.0	36.7273	1
CAACGCA	6900	0.0	34.06843	5
ATCAACG	7305	0.0	32.373985	3
AACGCAG	8020	0.0	30.470219	6
TCAACGC	7935	0.0	29.862904	4
TATCAAC	8590	0.0	28.297354	2
ACGCAGA	8895	0.0	26.310816	7
GTACATG	14710	0.0	25.256828	1
CGCAGAG	9535	0.0	24.54456	8
TACATGG	15175	0.0	23.608843	2
ACATGGG	14940	0.0	23.130508	3
CATGGGG	12235	0.0	19.790268	4
GCAGAGT	11500	0.0	19.451426	9
CTAACGC	665	0.0	18.382313	3
GTACTAG	2025	0.0	18.347061	1
GAGTACA	11055	0.0	17.314106	1
GTATAAG	2285	0.0	17.288507	1
ATGGGGG	8030	0.0	17.213257	5
AGAGTAC	9460	0.0	17.089315	10-11
>>END_MODULE