FastQCFastQC Report
Mon 16 Jan 2017
ERR1146608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062895
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122250.24146264143340915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106990.21132178328801998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56690.11197151037104267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81250.056.1435171
GTATCAA149550.044.967721
CAACGCA186750.035.3856435
ATCAACG187800.035.081033
TATCAAC196550.033.974552
TCAACGC195450.033.8282474
AACGCAG200800.033.260156
ACGCAGA220850.029.9624737
CGCAGAG228700.028.749088
GCAGAGT255450.025.4073969
GTACATG140800.023.4135151
GTGGTAT31400.022.7648721
GTACTAG20100.022.6948321
GAGTACT175200.022.35900512-13
TACATGG145250.022.3393922
AGAGTAC231700.022.12969410-11
CAGAGTA243700.022.06211710-11
ACATGGG144750.021.8313453
GTACTTT189650.020.5562814-15
TGATCGC10900.020.2648689
Produced by FastQC (version 0.11.5)