##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5062895 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34928158691816 33.0 31.0 34.0 30.0 34.0 2 32.407827339891504 34.0 31.0 34.0 30.0 34.0 3 32.51986284526936 34.0 31.0 34.0 30.0 34.0 4 36.02999193149374 37.0 35.0 37.0 35.0 37.0 5 36.0689852347323 37.0 35.0 37.0 35.0 37.0 6 36.133121860121534 37.0 36.0 37.0 35.0 37.0 7 36.09852584341567 37.0 36.0 37.0 35.0 37.0 8 36.10058948486982 37.0 36.0 37.0 35.0 37.0 9 37.88053258066778 39.0 38.0 39.0 35.0 39.0 10-11 37.83232547781457 39.0 38.0 39.0 35.0 39.0 12-13 37.80496395836769 39.0 38.0 39.0 35.0 39.0 14-15 39.1630774487719 40.0 39.0 41.0 36.0 41.0 16-17 39.107328119583755 40.0 39.0 41.0 36.0 41.0 18-19 39.20451757344365 40.0 39.0 41.0 36.0 41.0 20-21 39.26530315165533 40.5 39.0 41.0 36.0 41.0 22-23 39.23471185951911 40.0 39.0 41.0 36.0 41.0 24-25 39.22901502006263 41.0 39.0 41.0 36.0 41.0 26-27 39.157488847783725 40.5 39.0 41.0 36.0 41.0 28-29 39.101467144785744 40.0 39.0 41.0 35.0 41.0 30-31 38.97278928360157 40.0 38.5 41.0 35.0 41.0 32-33 38.88049515148941 40.0 38.0 41.0 35.0 41.0 34-35 38.819369352909746 40.0 38.0 41.0 35.0 41.0 36-37 38.756824603314904 40.0 38.0 41.0 35.0 41.0 38-39 38.677816051883354 40.0 38.0 41.0 35.0 41.0 40-41 38.58749006250376 40.0 38.0 41.0 35.0 41.0 42-43 38.49054602159437 40.0 38.0 41.0 34.5 41.0 44-45 38.353006333333 40.0 38.0 41.0 34.0 41.0 46-47 38.13461566949344 40.0 38.0 41.0 33.5 41.0 48-49 37.87018365974408 40.0 38.0 41.0 33.0 41.0 50-51 37.76787035875719 40.0 37.0 41.0 33.0 41.0 52-53 37.66839891010973 40.0 37.0 41.0 33.0 41.0 54-55 37.51306110436815 40.0 37.0 41.0 33.0 41.0 56-57 37.36171241947542 40.0 36.0 41.0 32.5 41.0 58-59 37.18639316043489 39.0 36.0 41.0 32.0 41.0 60-61 36.98752423267716 39.0 35.0 41.0 32.0 41.0 62-63 36.74943880921884 39.0 35.0 41.0 32.0 41.0 64-65 36.47622960776394 38.0 35.0 40.5 31.0 41.0 66-67 36.2064544099769 37.5 35.0 40.0 31.0 41.0 68-69 35.91080528037813 37.0 35.0 40.0 31.0 41.0 70-71 35.602322189182274 36.5 35.0 39.0 31.0 41.0 72-73 35.27826036289514 36.0 35.0 39.0 31.0 41.0 74-75 34.95815457361846 35.5 35.0 38.5 30.0 40.5 76-77 34.656973332451095 35.0 34.5 37.0 30.0 39.5 78-79 34.3720251950712 35.0 34.0 37.0 30.0 39.0 80-81 34.10662595214793 35.0 34.0 36.5 30.0 39.0 82-83 33.854999659285845 35.0 34.0 36.0 30.0 37.5 84-85 33.64840126054362 35.0 34.0 36.0 30.0 37.0 86-87 33.4590906981085 35.0 34.0 35.0 29.0 37.0 88-89 33.265623324204824 35.0 34.0 35.0 29.0 36.0 90-91 33.18373114591553 35.0 34.0 35.0 29.0 36.0 92-93 33.08617391828193 35.0 34.0 35.0 29.0 36.0 94-95 33.01823344153888 35.0 34.0 35.0 29.0 36.0 96-97 32.96294550844922 35.0 34.0 35.0 29.0 35.0 98-99 32.89386368866034 35.0 34.0 35.0 29.0 35.0 100 32.1720995596393 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3466386665542842 1101 2 0.3317241155253612 1101 3 0.3271450325207539 1101 4 0.19393551491178584 1101 5 0.2288250743094693 1101 6 0.25812395306916613 1101 7 0.2635563340262337 1101 8 0.26666468830109835 1101 9 0.364694769386098 1101 10-11 0.31759418757018665 1101 12-13 0.3530388908800717 1101 14-15 0.44701540694565267 1101 16-17 0.4976052694603368 1101 18-19 0.4560000447159993 1101 20-21 0.42131569067348806 1101 22-23 0.36221952348400066 1101 24-25 0.3827830643013783 1101 26-27 0.3578998124092152 1101 28-29 0.4253886325018712 1101 30-31 0.4915659113488715 1101 32-33 0.4398267182386846 1101 34-35 0.43098320198191686 1101 36-37 0.4662010368118743 1101 38-39 0.48733396963785935 1101 40-41 0.44084109245835634 1101 42-43 0.4809471927810165 1101 44-45 0.49023517276665984 1101 46-47 0.4855356091876075 1101 48-49 0.5389184610110362 1101 50-51 0.5763042151121667 1101 52-53 0.5704696935146885 1101 54-55 0.5460412824316307 1101 56-57 0.5057890326333023 1101 58-59 0.4845372757104016 1101 60-61 0.48775182648429904 1101 62-63 0.4764069920849394 1101 64-65 0.47094696487138776 1101 66-67 0.42533884895861007 1101 68-69 0.42859440979740526 1101 70-71 0.398715700778709 1101 72-73 0.40737323230122513 1101 74-75 0.3753844154197239 1101 76-77 0.3672713195429864 1101 78-79 0.42756433972537167 1101 80-81 0.38118560539335533 1101 82-83 0.42927221887243405 1101 84-85 0.46138464946092483 1101 86-87 0.39967913272570144 1101 88-89 0.4806705640965774 1101 90-91 0.47893364465742394 1101 92-93 0.49243675651592156 1101 94-95 0.4466953874534596 1101 96-97 0.4321515015232791 1101 98-99 0.47289477634234345 1101 100 0.4982189130654504 1102 1 0.2995205285669016 1102 2 0.3033240375177044 1102 3 0.28327818592207166 1102 4 0.1964970801771173 1102 5 0.20720287293701745 1102 6 0.24411386218706355 1102 7 0.223237417394067 1102 8 0.2094345223518701 1102 9 0.29970758748706317 1102 10-11 0.27681198445303323 1102 12-13 0.30433093583550175 1102 14-15 0.4254745408882741 1102 16-17 0.5096236725862653 1102 18-19 0.40388425928797744 1102 20-21 0.38876721330107245 1102 22-23 0.4028437955594484 1102 24-25 0.4010920944834737 1102 26-27 0.3774489907104126 1102 28-29 0.41116668533044987 1102 30-31 0.48391827051714387 1102 32-33 0.39873866251862466 1102 34-35 0.4350391959438724 1102 36-37 0.4218783611226229 1102 38-39 0.4154228124610384 1102 40-41 0.3931987493596196 1102 42-43 0.4191338116567209 1102 44-45 0.42904400348831473 1102 46-47 0.37503392739601793 1102 48-49 0.4275388616050293 1102 50-51 0.41425569737040036 1102 52-53 0.42932962163998667 1102 54-55 0.40336690965816047 1102 56-57 0.38613633244004575 1102 58-59 0.3372685513862095 1102 60-61 0.31936061250136305 1102 62-63 0.3753836254498637 1102 64-65 0.3618069370118917 1102 66-67 0.3368316748126077 1102 68-69 0.32467657981035103 1102 70-71 0.31694991175125153 1102 72-73 0.29214236926790704 1102 74-75 0.31796310816565665 1102 76-77 0.24875916982342972 1102 78-79 0.2516376441226882 1102 80-81 0.291518524692151 1102 82-83 0.2828728941610379 1102 84-85 0.24187001381065443 1102 86-87 0.27208318058612946 1102 88-89 0.2886119334651056 1102 90-91 0.25469648970443615 1102 92-93 0.2665023648822 1102 94-95 0.2976731148347227 1102 96-97 0.31432115651703896 1102 98-99 0.30790168155812836 1102 100 0.2917361238700167 1103 1 0.22812885368885105 1103 2 0.21663480446856198 1103 3 0.1988093987730295 1103 4 0.1611589502551496 1103 5 0.11860543405797586 1103 6 0.1503451984811619 1103 7 0.13752926936815157 1103 8 0.12264112279821404 1103 9 0.22675263671124668 1103 10-11 0.22734826682702192 1103 12-13 0.19599608475857622 1103 14-15 0.3487555211482629 1103 16-17 0.42261623226990963 1103 18-19 0.33045634328743034 1103 20-21 0.311931249471634 1103 22-23 0.3293886380072806 1103 24-25 0.3273790502426692 1103 26-27 0.3289718712787746 1103 28-29 0.31155879503555894 1103 30-31 0.3234798008800581 1103 32-33 0.31894309868855686 1103 34-35 0.3358709972642373 1103 36-37 0.3460607772977724 1103 38-39 0.35094971336354064 1103 40-41 0.36975934001872446 1103 42-43 0.3792125501186234 1103 44-45 0.29386188982411454 1103 46-47 0.32001280351856565 1103 48-49 0.3706110885577729 1103 50-51 0.3445563065952726 1103 52-53 0.40045552275160645 1103 54-55 0.3887320346605634 1103 56-57 0.3341388910703529 1103 58-59 0.29858418601317993 1103 60-61 0.2914896513208305 1103 62-63 0.2699833380904124 1103 64-65 0.25089049489344006 1103 66-67 0.18261991040584746 1103 68-69 0.20528937721675078 1103 70-71 0.17594848377505912 1103 72-73 0.19705981958320962 1103 74-75 0.19484874242422734 1103 76-77 0.15179111560184566 1103 78-79 0.1821368572310078 1103 80-81 0.22368265063009574 1103 82-83 0.18467812692582442 1103 84-85 0.1789512398153903 1103 86-87 0.16526762975935583 1103 88-89 0.26297231480531735 1103 90-91 0.18465372565726312 1103 92-93 0.19798979770583003 1103 94-95 0.25185314931992764 1103 96-97 0.18166037817661618 1103 98-99 0.1700431503084303 1103 100 0.22181432528419265 1104 1 0.21389845407852448 1104 2 0.19647771831307637 1104 3 0.20241443224392697 1104 4 0.16178550120530133 1104 5 0.12951252185903428 1104 6 0.14198282082932678 1104 7 0.15656432330995074 1104 8 0.17067993193178665 1104 9 0.20569698680964876 1104 10-11 0.2489498347265453 1104 12-13 0.23162029899868486 1104 14-15 0.37574044936848594 1104 16-17 0.39404670198153724 1104 18-19 0.31719946315737246 1104 20-21 0.308156927940324 1104 22-23 0.31906589227424575 1104 24-25 0.24386367567792888 1104 26-27 0.32904435865724935 1104 28-29 0.29743009167925294 1104 30-31 0.3522990464847382 1104 32-33 0.2857573318856339 1104 34-35 0.2750551152055607 1104 36-37 0.2318707353028202 1104 38-39 0.2480384935350699 1104 40-41 0.2517393150718661 1104 42-43 0.2814504436568015 1104 44-45 0.22088721679896395 1104 46-47 0.2601469504219267 1104 48-49 0.3387431357132371 1104 50-51 0.2579927596010734 1104 52-53 0.24089924259652662 1104 54-55 0.2255846672441777 1104 56-57 0.21284167142619026 1104 58-59 0.2564259874520687 1104 60-61 0.2433389433964237 1104 62-63 0.2470728834522049 1104 64-65 0.2867919466156934 1104 66-67 0.2681715549889603 1104 68-69 0.23689091220486347 1104 70-71 0.23079411281081263 1104 72-73 0.24654212311855872 1104 74-75 0.27203299036561646 1104 76-77 0.2121022189239241 1104 78-79 0.2153190449181963 1104 80-81 0.20684978134762844 1104 82-83 0.16527520570915755 1104 84-85 0.2368610444532635 1104 86-87 0.27131732269591424 1104 88-89 0.22346344353500314 1104 90-91 0.17462681235249988 1104 92-93 0.20490197525629839 1104 94-95 0.23880628511898294 1104 96-97 0.22481455825467833 1104 98-99 0.220528799288509 1104 100 0.2593874708908501 1105 1 0.13822781039807808 1105 2 0.11142111843070523 1105 3 0.16723361433361816 1105 4 0.1048618499400149 1105 5 0.08979454738626913 1105 6 0.13579836708311888 1105 7 0.1356803225155616 1105 8 0.1099599447641495 1105 9 0.11346428949298826 1105 10-11 0.15255931845312887 1105 12-13 0.186645257178931 1105 14-15 0.240201060509726 1105 16-17 0.2732880172222636 1105 18-19 0.23654902280875234 1105 20-21 0.16727769154224603 1105 22-23 0.1540483050638315 1105 24-25 0.2176362468860802 1105 26-27 0.1996711393384416 1105 28-29 0.23295893012133462 1105 30-31 0.2712956684418302 1105 32-33 0.2428949990691649 1105 34-35 0.2389733108074239 1105 36-37 0.22837806999068277 1105 38-39 0.22212480835569437 1105 40-41 0.2909913048518078 1105 42-43 0.29858880783618247 1105 44-45 0.265663996716313 1105 46-47 0.2907374300314558 1105 48-49 0.411279207635566 1105 50-51 0.3943732219480225 1105 52-53 0.35712373063874736 1105 54-55 0.34484399935028875 1105 56-57 0.28052063704615193 1105 58-59 0.2962420187385888 1105 60-61 0.2968010077262804 1105 62-63 0.2988619667785386 1105 64-65 0.2986612608481636 1105 66-67 0.28957343324956497 1105 68-69 0.25446571036250276 1105 70-71 0.25984652307975864 1105 72-73 0.25922840242102296 1105 74-75 0.2944591660925937 1105 76-77 0.22945039795065014 1105 78-79 0.20049871708189215 1105 80-81 0.21788752224085783 1105 82-83 0.22023896192483505 1105 84-85 0.1974946709704284 1105 86-87 0.23775391707869886 1105 88-89 0.3031079661023952 1105 90-91 0.21583678760437408 1105 92-93 0.20179074808530117 1105 94-95 0.22996050070267415 1105 96-97 0.236268109687245 1105 98-99 0.2551568422005346 1105 100 0.24519300713845382 1106 1 0.07725624762804273 1106 2 0.07807274544522613 1106 3 0.07330797376219067 1106 4 0.08460052489112257 1106 5 0.07284912587123671 1106 6 0.09551805800290225 1106 7 0.07638792589284549 1106 8 0.10185366844456212 1106 9 0.11168957951051084 1106 10-11 0.07478430016864479 1106 12-13 0.05886539374964883 1106 14-15 0.17405954672265977 1106 16-17 0.22988597343685768 1106 18-19 0.1794343535441172 1106 20-21 0.15608271686499364 1106 22-23 0.1399142776538156 1106 24-25 0.14245434451188288 1106 26-27 0.17428555936437107 1106 28-29 0.17589453679723732 1106 30-31 0.16598784722385318 1106 32-33 0.15999980304186323 1106 34-35 0.14399131696657008 1106 36-37 0.15288686958348308 1106 38-39 0.16706569991631426 1106 40-41 0.13587571161200884 1106 42-43 0.1296064846558096 1106 44-45 0.14241289617960717 1106 46-47 0.1235075133428154 1106 48-49 0.18085611991089223 1106 50-51 0.10989438499128568 1106 52-53 0.08629684547482697 1106 54-55 0.11788214157748911 1106 56-57 0.05793944177453625 1106 58-59 0.06570488161131038 1106 60-61 0.0657250029963734 1106 62-63 0.0928333220590929 1106 64-65 0.10577055437578053 1106 66-67 0.11263983183993531 1106 68-69 0.08884628070818934 1106 70-71 0.05169780869076135 1106 72-73 0.09789746115879439 1106 74-75 0.08314695845938047 1106 76-77 0.15663625872901576 1106 78-79 0.13779762844627896 1106 80-81 0.1506568515527391 1106 82-83 0.07302590689860011 1106 84-85 0.05987766491246305 1106 86-87 0.07043329704537626 1106 88-89 0.12683643208672635 1106 90-91 0.036433055977177276 1106 92-93 0.07495065657396793 1106 94-95 0.0514938042545765 1106 96-97 0.05739142369553463 1106 98-99 0.06956439455719021 1106 100 0.10142018178232348 1107 1 0.12014400043698714 1107 2 0.09133517171708405 1107 3 0.0867127713550957 1107 4 0.0744390672755415 1107 5 0.13499621363848036 1107 6 0.11319406851042402 1107 7 0.15752649779948769 1107 8 0.12628272573682864 1107 9 0.146595359595608 1107 10-11 0.12852066153288888 1107 12-13 0.1781645362496178 1107 14-15 0.2537923964930542 1107 16-17 0.261247172977626 1107 18-19 0.2070709427134645 1107 20-21 0.18454028161884395 1107 22-23 0.17413917252969924 1107 24-25 0.18497779639002943 1107 26-27 0.1931601473238871 1107 28-29 0.16330775597164404 1107 30-31 0.19904005200800157 1107 32-33 0.19672507248751003 1107 34-35 0.18263252588467793 1107 36-37 0.16793946269635995 1107 38-39 0.1662760804656429 1107 40-41 0.1632633662395122 1107 42-43 0.19854524871884394 1107 44-45 0.19935780622807897 1107 46-47 0.2694928326263053 1107 48-49 0.35649260728960286 1107 50-51 0.2976013991394879 1107 52-53 0.2365634816213955 1107 54-55 0.3108242468504798 1107 56-57 0.2704761901923689 1107 58-59 0.23562781972154312 1107 60-61 0.2528955185498418 1107 62-63 0.23711363897020732 1107 64-65 0.23768968424096215 1107 66-67 0.22567212625774147 1107 68-69 0.21914512380883622 1107 70-71 0.18864481171307546 1107 72-73 0.1923035342125985 1107 74-75 0.23039181782139906 1107 76-77 0.24729852116119133 1107 78-79 0.22783918965966166 1107 80-81 0.2048121557812479 1107 82-83 0.1624759886480689 1107 84-85 0.204125407353267 1107 86-87 0.17248703312277058 1107 88-89 0.25138381092096296 1107 90-91 0.22942766880468213 1107 92-93 0.2266043825529067 1107 94-95 0.21619285443944847 1107 96-97 0.2634225942025239 1107 98-99 0.22368470229909576 1107 100 0.23479743141317044 1108 1 0.030083168160601303 1108 2 0.003338092494743705 1108 3 0.017045521304304145 1108 4 -0.021802304405817097 1108 5 -0.02896042197286164 1108 6 0.032474661958964646 1108 7 0.010187831274038217 1108 8 0.039902227591625206 1108 9 0.06257280842336144 1108 10-11 0.06998730395935127 1108 12-13 0.034979176220232944 1108 14-15 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86-87 -0.21181579851970156 2214 88-89 -0.17312391668888694 2214 90-91 -0.22497468646856333 2214 92-93 -0.16691400935271616 2214 94-95 -0.11083671499608982 2214 96-97 -0.17638919351126958 2214 98-99 -0.2039997968169729 2214 100 -0.3526796730611039 2215 1 -0.35534190818416533 2215 2 -0.3537135835079255 2215 3 -0.35787106756234977 2215 4 -0.2602590067373569 2215 5 -0.24957383033003566 2215 6 -0.2819928668886149 2215 7 -0.299211054450609 2215 8 -0.2959475864719323 2215 9 -0.3276428039691197 2215 10-11 -0.2840618371060728 2215 12-13 -0.3259615491221979 2215 14-15 -0.3730820758155531 2215 16-17 -0.37301427244697294 2215 18-19 -0.41387739658691913 2215 20-21 -0.4661232686888823 2215 22-23 -0.43385569819456293 2215 24-25 -0.42498258061807803 2215 26-27 -0.4432096126716871 2215 28-29 -0.4512483517535273 2215 30-31 -0.4260045422405625 2215 32-33 -0.4831785214657742 2215 34-35 -0.48725660990098874 2215 36-37 -0.483148879187695 2215 38-39 -0.4699402453258372 2215 40-41 -0.4545881742733471 2215 42-43 -0.46584469388678684 2215 44-45 -0.5145459970716288 2215 46-47 -0.4877208818136438 2215 48-49 -0.37432955897874365 2215 50-51 -0.37533371269648796 2215 52-53 -0.3940775105999066 2215 54-55 -0.3796488904853845 2215 56-57 -0.4376674577872919 2215 58-59 -0.48159706562813653 2215 60-61 -0.4139250773319745 2215 62-63 -0.468474021817066 2215 64-65 -0.4192389038298643 2215 66-67 -0.41038569026482463 2215 68-69 -0.3623995759780101 2215 70-71 -0.3756944090799266 2215 72-73 -0.3301226870325422 2215 74-75 -0.38139228084709487 2215 76-77 -0.3060266555433415 2215 78-79 -0.3789812505099377 2215 80-81 -0.3382417529413999 2215 82-83 -0.33234805078057406 2215 84-85 -0.3522075094018291 2215 86-87 -0.2776922666105648 2215 88-89 -0.26080077091676657 2215 90-91 -0.28686340461766235 2215 92-93 -0.26100271284908416 2215 94-95 -0.24672136889108742 2215 96-97 -0.3215192270998344 2215 98-99 -0.27728333748018485 2215 100 -0.4303936217089195 2216 1 -0.4507775111638779 2216 2 -0.509971893884579 2216 3 -0.4743066387150847 2216 4 -0.32874175961151053 2216 5 -0.33212118842593696 2216 6 -0.33445642942331233 2216 7 -0.37549083035334974 2216 8 -0.3428860636042472 2216 9 -0.40165081906217637 2216 10-11 -0.4401445134186659 2216 12-13 -0.41549314888738564 2216 14-15 -0.4684216950500115 2216 16-17 -0.46670622137175855 2216 18-19 -0.49259552761856895 2216 20-21 -0.5092731699810642 2216 22-23 -0.5183974361772314 2216 24-25 -0.5667921242457936 2216 26-27 -0.5689786761452851 2216 28-29 -0.5863809608316402 2216 30-31 -0.5124405322274441 2216 32-33 -0.5896840158182641 2216 34-35 -0.5724626199388609 2216 36-37 -0.5649998896057156 2216 38-39 -0.5903369928742777 2216 40-41 -0.5360465746496104 2216 42-43 -0.5004285252533265 2216 44-45 -0.5354209449107543 2216 46-47 -0.6024982233948748 2216 48-49 -0.4511798223469583 2216 50-51 -0.4882556300771981 2216 52-53 -0.47824308247759717 2216 54-55 -0.473525982887935 2216 56-57 -0.5139322319130173 2216 58-59 -0.548838842775659 2216 60-61 -0.5266073254391586 2216 62-63 -0.5486393684747028 2216 64-65 -0.41043510961247875 2216 66-67 -0.4693624662983993 2216 68-69 -0.473095185446887 2216 70-71 -0.44121035748840143 2216 72-73 -0.4084055399848552 2216 74-75 -0.4213481408119435 2216 76-77 -0.43105595183124734 2216 78-79 -0.3918726836167039 2216 80-81 -0.48307121782386986 2216 82-83 -0.43794151997382613 2216 84-85 -0.38620735446635024 2216 86-87 -0.31157395955805356 2216 88-89 -0.27360371840402564 2216 90-91 -0.3558632684689442 2216 92-93 -0.3546484353037229 2216 94-95 -0.3402727044746996 2216 96-97 -0.38134881133346 2216 98-99 -0.3628933345980698 2216 100 -0.4446865854721409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 36.0 8 148.0 9 232.0 10 238.0 11 197.0 12 205.0 13 352.0 14 535.0 15 982.0 16 1951.0 17 3462.0 18 5840.0 19 8884.0 20 13048.0 21 19482.0 22 28244.0 23 33054.0 24 32383.0 25 28862.0 26 27707.0 27 31806.0 28 39512.0 29 48491.0 30 60951.0 31 77836.0 32 99537.0 33 131008.0 34 188120.0 35 288995.0 36 522692.0 37 1188691.0 38 1634035.0 39 544075.0 40 1301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02223919139981 19.822216042035453 13.694567444645958 24.460977321918776 2 15.164739572695932 20.719897888841526 40.059825571955614 24.055536966506924 3 17.885329062252424 26.182492321080836 30.575005920535066 25.357172696131673 4 12.450445768240039 17.92046621142941 38.89994101216019 30.72914700817036 5 12.96876675833569 38.07616330872827 35.35840691446338 13.59666301847266 6 29.828644975642188 38.58499318637069 18.068255598306575 13.518106239680547 7 25.994559633284837 33.120602564462146 23.0874476139536 17.79739018829942 8 25.34641946802848 35.346429343859654 20.647617505108272 18.659533683003595 9 26.235731231136032 15.203125959802033 20.786514745620735 37.7746280634412 10-11 24.494540323449556 26.29598125893079 28.723991023706514 20.485487393913143 12-13 25.424495273948995 24.057530326028882 28.488552498126076 22.029421901896047 14-15 22.660848254150345 25.036189783193123 26.570885460150944 25.732076502505585 16-17 21.978947372579285 27.93778486113694 27.19664594296906 22.88662182331472 18-19 21.75722631035883 27.050114480114907 29.499832901695633 21.69282630783063 20-21 22.834997387359955 26.24116697065992 29.23749130066416 21.686344341315966 22-23 22.68886674521198 26.082419248275933 28.944309530416884 22.284404476095197 24-25 22.290853355639413 26.29958748897617 29.257341896286608 22.15221725909781 26-27 22.33616906717452 26.478884478361852 28.956907392955838 22.228039061507793 28-29 22.097181760354676 26.634675085566172 29.02658745901184 22.241555695067316 30-31 22.519978482621912 26.30928080622815 28.93081997031534 22.2399207408346 32-33 22.524238151915725 26.4917479872152 28.641519876212897 22.34249398465618 34-35 22.701832245301173 26.50362594539696 28.800764149067927 21.993777660233942 36-37 22.638496203207197 26.405898684575803 28.717127356965875 22.238477755251125 38-39 22.580791387624448 26.400415654885567 28.8349614663982 22.183831491091784 40-41 22.58654039302237 26.18818546882626 28.857196920243016 22.36807721790835 42-43 22.56614051841881 26.181976912418687 28.605333509780472 22.646549059382036 44-45 22.647156158300067 26.51982464125892 28.427474607577487 22.405544592863528 46-47 22.73462119992613 26.484007667549893 27.95532990512345 22.82604122740053 48-49 23.010590491735897 26.536066699447673 27.79652255959318 22.656820249223244 50-51 22.840074700344367 26.74402688580348 27.569611852507308 22.846286561344844 52-53 22.79522686079358 26.855737161910127 27.44987604371312 22.89915993358317 54-55 22.77675996575464 26.771248868355542 27.409216363309746 23.042774802580077 56-57 22.89417311646232 26.73356394617971 27.376803726490806 22.99545921086716 58-59 22.637635862158326 26.639174915640435 27.74318704161212 22.980002180589125 60-61 22.730172631572295 26.73836917965191 27.613220029852677 22.918238158923124 62-63 22.649852801317827 26.603724951287656 27.558299270080198 23.18812297731432 64-65 22.493075082225992 26.58103812931622 27.61578596829154 23.310100820166248 66-67 22.637358209494714 26.535131770929315 27.53601289711168 23.291497122464293 68-69 22.52768562103658 26.441640825305218 27.68203605073614 23.34863750292206 70-71 22.530549747310047 26.318222633419232 27.59674333550739 23.554484283763333 72-73 22.630960134827845 26.428360857063428 27.546132266983225 23.394546741125506 74-75 22.56665083210459 26.569779557218236 27.38364312781652 23.479926482860648 76-77 22.583519762403178 26.515457975536343 27.263063894440393 23.637958367620087 78-79 22.43152000590974 26.53681370632901 27.299045652144283 23.732620635616964 80-81 22.352491533910282 26.61757371596405 27.37725527840572 23.65267947171995 82-83 22.509773620182887 26.38790542031258 27.649513723756698 23.452807235747837 84-85 22.688445900844098 26.436135820712597 27.21144774634049 23.66397053210282 86-87 22.246935794639235 26.57940763140456 27.363731619952613 23.809924954003588 88-89 22.509295571012235 26.29259544193589 27.24379036104837 23.954318626003502 90-91 22.381150931127898 26.348468424765926 27.28833638955759 23.982044254548587 92-93 22.383771400084022 26.434344106694812 27.280412428556104 23.901472064665054 94-95 22.03428931922953 26.330334946147836 27.42652736673936 24.208848367883274 96-97 22.132518012496462 26.243127915093282 27.34718462280515 24.277169449605104 98-99 22.026055089506226 26.36493242591975 27.212906941650694 24.396105542923326 100 22.19154292382828 26.179368541896782 27.076891207768583 24.552197326506363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 104.0 1 71.0 2 155.5 3 291.5 4 214.0 5 184.0 6 408.0 7 1033.0 8 2024.5 9 2515.5 10 2074.5 11 1654.0 12 1728.5 13 2001.0 14 2360.0 15 2784.5 16 3248.5 17 3784.0 18 4455.0 19 5302.0 20 6300.0 21 7435.0 22 9040.0 23 11201.0 24 14013.0 25 18277.0 26 24280.0 27 31375.0 28 38625.5 29 47585.5 30 57951.0 31 66916.5 32 77783.5 33 88839.0 34 97123.5 35 105457.0 36 117046.0 37 132787.5 38 141736.5 39 145898.5 40 150855.5 41 152643.0 42 156199.0 43 163622.5 44 172440.0 45 184681.0 46 193062.5 47 197537.0 48 203030.5 49 202051.0 50 197479.5 51 196614.0 52 194476.5 53 189123.0 54 183116.0 55 170516.0 56 155376.0 57 137601.5 58 118486.5 59 103464.5 60 86518.0 61 69071.5 62 54578.5 63 42473.5 64 31459.0 65 22117.0 66 15583.5 67 10948.0 68 7792.0 69 5825.5 70 4599.5 71 3487.0 72 2638.5 73 2045.0 74 1597.0 75 1192.0 76 878.0 77 648.0 78 477.0 79 282.5 80 112.5 81 63.0 82 43.5 83 28.5 84 13.0 85 8.5 86 10.0 87 8.5 88 4.0 89 1.0 90 1.0 91 1.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0023899369826946835 2 0.003496023520140157 3 1.580123624922105E-4 4 0.04997140963816157 5 0.002291179256137052 6 0.0048983832372585245 7 1.9751545311526313E-5 8 5.925463593457893E-4 9 3.9503090623052625E-5 10-11 6.913040859034209E-5 12-13 0.0 14-15 3.9503090623052625E-5 16-17 2.2714277108255257E-4 18-19 1.3826081718068417E-4 20-21 1.6788813514797364E-4 22-23 0.0 24-25 0.0 26-27 1.580123624922105E-4 28-29 9.875772655763156E-6 30-31 2.0739122577102627E-4 32-33 1.777639078037368E-4 34-35 1.6788813514797364E-4 36-37 6.913040859034209E-5 38-39 8.098133577725788E-4 40-41 5.530432687227367E-4 42-43 0.0 44-45 3.259004976401841E-4 46-47 0.0 48-49 8.88819539018684E-5 50-51 0.0 52-53 4.2465822419781565E-4 54-55 3.851551335747631E-4 56-57 1.6788813514797364E-4 58-59 8.493164483956313E-4 60-61 6.32049449968842E-4 62-63 1.975154531152631E-4 64-65 3.7527936091899994E-4 66-67 0.0011060865374454734 68-69 0.0022418003928582363 70-71 0.0033380111576479464 72-73 0.0028145952068924993 74-75 0.0025677008904984202 76-77 0.0039700606076167886 78-79 0.0013036019905607365 80-81 9.875772655763154E-4 82-83 6.32049449968842E-4 84-85 5.826705866900262E-4 86-87 0.0 88-89 0.0 90-91 5.9254635934578935E-5 92-93 1.1850927186915787E-4 94-95 1.2838504452492103E-4 96-97 4.1478245154205254E-4 98-99 1.3826081718068417E-4 100 0.07400904028228908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5062895.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.99194626388669 #Duplication Level Percentage of deduplicated Percentage of total 1 66.98114564646185 34.82480125144412 2 16.452765929044734 17.10822644150399 3 6.795968104285191 10.600068254672507 4 3.502657727210152 7.284399693355907 5 1.9614115308292495 5.098880145612103 6 1.2350938610807847 3.85289601937011 7 0.7704712915270793 2.80408113908603 8 0.5317643525083507 2.2117970912531737 9 0.38084909044582804 1.782097690059865 >10 1.3484375164970248 10.865424193950837 >50 0.027826384763668378 0.97827573847763 >100 0.010121918833574209 0.9666587205430285 >500 7.433232564363922E-4 0.250396278279736 >1k 5.405987316882705E-4 0.36742588427250317 >5k 6.757484146103381E-5 0.1991716712507956 >10k+ 1.3514968292206763E-4 0.8053997868677436 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12225 0.24146264143340915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10699 0.21132178328801998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5669 0.11197151037104267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.950309062305262E-5 2 0.0 0.0 0.0 0.0 5.9254635934578935E-5 3 0.0 0.0 0.0 0.0 5.9254635934578935E-5 4 0.0 0.0 0.0 0.0 7.900618124610524E-5 5 0.0 3.950309062305262E-5 0.0 0.0 7.900618124610524E-5 6 0.0 3.950309062305262E-5 0.0 0.0 1.1850927186915787E-4 7 0.0 3.950309062305262E-5 0.0 0.0 1.1850927186915787E-4 8 0.0 3.950309062305262E-5 0.0 0.0 1.777639078037368E-4 9 0.0 3.950309062305262E-5 0.0 0.0 1.975154531152631E-4 10-11 0.0 3.950309062305262E-5 0.0 1.5801236249221047E-4 1.975154531152631E-4 12-13 0.0 3.950309062305262E-5 0.0 1.5801236249221047E-4 1.975154531152631E-4 14-15 0.0 5.9254635934578935E-5 0.0 1.5801236249221047E-4 3.1602472498442095E-4 16-17 0.0 5.9254635934578935E-5 0.0 1.5801236249221047E-4 4.5428554216510515E-4 18-19 0.0 8.88819539018684E-5 0.0 1.777639078037368E-4 5.13540178099684E-4 20-21 0.0 1.1850927186915787E-4 0.0 2.172669984267894E-4 5.13540178099684E-4 22-23 0.0 1.1850927186915787E-4 0.0 3.357762702959473E-4 5.826705866900262E-4 24-25 0.0 1.3826081718068417E-4 0.0 4.740370874766314E-4 6.814283132476577E-4 26-27 0.0 1.3826081718068417E-4 0.0 8.098133577725788E-4 6.913040859034209E-4 28-29 0.0 1.3826081718068417E-4 0.0 0.0022714277108255255 6.913040859034209E-4 30-31 0.0 1.3826081718068417E-4 0.0 0.007485835673068471 7.406829491822366E-4 32-33 0.0 1.3826081718068417E-4 0.0 0.023593220874618178 8.295649030841051E-4 34-35 0.0 1.5801236249221047E-4 0.0 0.04878631691946999 9.184468569859735E-4 36-37 0.0 1.5801236249221047E-4 0.0 0.0920422011517126 9.283226296417366E-4 38-39 0.0 1.5801236249221047E-4 0.0 0.1620811808263849 9.381984022974998E-4 40-41 0.0 1.975154531152631E-4 0.0 0.24051456725845588 9.48074174953263E-4 42-43 0.0 1.975154531152631E-4 0.0 0.32338217561296456 9.57949947609026E-4 44-45 0.0 1.975154531152631E-4 0.0 0.42543841023762097 9.678257202647892E-4 46-47 0.0 2.172669984267894E-4 0.0 0.5538037032172305 0.0010468319015108944 48-49 0.0 2.3701854373831574E-4 0.0 0.6936643955681483 0.0011258380827569997 50-51 0.0 2.56770089049842E-4 0.0 0.8418997431311532 0.0011258380827569997 52-53 0.0 2.56770089049842E-4 0.0 0.992268257587803 0.001214720036658868 54-55 0.0 2.666458617056052E-4 0.0 1.163810428618409 0.0013134777632164995 56-57 0.0 2.7652163436136834E-4 0.0 1.3673303515083761 0.001333229308528026 58-59 0.0 2.7652163436136834E-4 0.0 1.6009516294531094 0.0013628566264953154 60-61 0.0 2.962731796728947E-4 0.0 1.851144453914213 0.0013826081718068418 62-63 0.0 2.962731796728947E-4 0.0 2.104951811167326 0.0013826081718068418 64-65 0.0 3.061489523286578E-4 0.0 2.3732172995884766 0.0013826081718068418 66-67 0.0 3.4565204295171044E-4 0.0 2.679297121508544 0.0014221112624298944 68-69 0.0 3.555278156074736E-4 0.0 3.021759289892443 0.0014616143530529469 70-71 0.0 3.555278156074736E-4 0.0 3.393325360292876 0.001530744761643289 72-73 0.0 3.555278156074736E-4 0.0 3.7767917367435038 0.001896148349906526 74-75 0.0 3.555278156074736E-4 0.0 4.161166684278461 0.001896148349906526 76-77 0.0 3.752793609189999E-4 0.0 4.590822049440093 0.001896148349906526 78-79 0.0 3.950309062305262E-4 0.0 5.060039759860712 0.001896148349906526 80-81 0.0 4.049066788862894E-4 0.0 5.568632175859859 0.002004781849119921 82-83 0.0 4.2465822419781565E-4 0.0 6.091109928213008 0.0021825457569236576 84-85 0.0 4.345339968535788E-4 0.0 6.609785508093689 0.0021924215295794206 86-87 0.0 4.345339968535788E-4 0.0 7.15589598441208 0.0021924215295794206 88 0.0 4.5428554216510515E-4 0.0 7.587595634513455 0.0022121730748909467 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8125 0.0 56.143517 1 GTATCAA 14955 0.0 44.96772 1 CAACGCA 18675 0.0 35.385643 5 ATCAACG 18780 0.0 35.08103 3 TATCAAC 19655 0.0 33.97455 2 TCAACGC 19545 0.0 33.828247 4 AACGCAG 20080 0.0 33.26015 6 ACGCAGA 22085 0.0 29.962473 7 CGCAGAG 22870 0.0 28.74908 8 GCAGAGT 25545 0.0 25.407396 9 GTACATG 14080 0.0 23.413515 1 GTGGTAT 3140 0.0 22.764872 1 GTACTAG 2010 0.0 22.694832 1 GAGTACT 17520 0.0 22.359005 12-13 TACATGG 14525 0.0 22.339392 2 AGAGTAC 23170 0.0 22.129694 10-11 CAGAGTA 24370 0.0 22.062117 10-11 ACATGGG 14475 0.0 21.831345 3 GTACTTT 18965 0.0 20.55628 14-15 TGATCGC 1090 0.0 20.264868 9 >>END_MODULE