FastQCFastQC Report
Mon 16 Jan 2017
ERR1146607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057881
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1447652.8621669825763No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC357750.7073120146559398No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC277700.5490441550522838No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA254750.5036694220366197No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG80680.1595134405099685No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA66460.1313989000532041No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG62180.12293685834047896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17500.028.212951
GAGTACG26200.026.2028681
GTACGCA26150.025.3463653
AGTACGC28900.022.9345152
CGCAGTG29550.022.7412096
TACGCAG30150.022.1391374
GCATCGT17950.021.9905958
TACGGGC12400.020.8497434
GGCGTCG12000.020.7547158
ATACGGG12950.019.9646263
TAACGCC7550.019.9233674
CATGGGG79350.019.9045354
CTCGTCG11500.019.6131959
CGGGCGT13100.019.3712446
ACGGGCG12900.019.3126355
GCTATTG26950.019.1803288
CTAACGC7700.018.9251463
TGATCGC12350.018.6437859
TAGGCAT29550.018.61165
GGGCTAT28050.018.4287076
Produced by FastQC (version 0.11.5)