##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146607_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5057881
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.848161314985465	31.0	31.0	34.0	27.0	34.0
2	30.885656859068057	31.0	31.0	34.0	27.0	34.0
3	31.029971444563444	31.0	31.0	34.0	27.0	34.0
4	34.552804623121816	37.0	35.0	37.0	31.0	37.0
5	34.55266029390569	37.0	35.0	37.0	31.0	37.0
6	34.77750109186041	37.0	35.0	37.0	32.0	37.0
7	34.66181252583839	37.0	35.0	37.0	31.0	37.0
8	34.66122591654489	37.0	35.0	37.0	31.0	37.0
9	36.2507006392598	39.0	37.0	39.0	31.0	39.0
10-11	36.25954248429332	39.0	37.0	39.0	31.0	39.0
12-13	36.24335161305693	39.0	37.0	39.0	31.0	39.0
14-15	37.38897564019399	40.0	37.5	41.0	31.0	41.0
16-17	37.436110003378886	40.0	38.0	41.0	31.0	41.0
18-19	37.45155639288468	40.0	38.0	41.0	31.0	41.0
20-21	37.55387008907485	40.0	38.0	41.0	31.0	41.0
22-23	37.505406809689674	40.0	38.0	41.0	31.0	41.0
24-25	37.46982204603074	40.0	37.5	41.0	31.0	41.0
26-27	37.45373428121381	40.0	37.0	41.0	30.5	41.0
28-29	37.45455903371392	40.0	37.0	41.0	30.5	41.0
30-31	37.45974707589997	40.0	37.0	41.0	30.0	41.0
32-33	37.38363071412712	40.0	37.0	41.0	30.0	41.0
34-35	37.2395832365372	40.0	37.0	41.0	30.0	41.0
36-37	37.11472363624213	40.0	37.0	41.0	30.0	41.0
38-39	37.0313858708815	40.0	37.0	41.0	30.0	41.0
40-41	37.06892599885209	40.0	37.0	41.0	30.0	41.0
42-43	36.86837274344731	40.0	36.0	41.0	30.0	41.0
44-45	36.65714733106611	39.5	36.0	41.0	29.0	41.0
46-47	36.46919016085985	39.0	35.0	41.0	28.5	41.0
48-49	36.31116449754354	39.0	35.0	41.0	28.0	41.0
50-51	36.10569179069259	39.0	35.0	40.0	28.0	41.0
52-53	36.081104419024484	39.0	35.0	40.0	28.0	41.0
54-55	35.94584767810868	39.0	35.0	40.0	28.0	41.0
56-57	35.576664516227254	38.0	34.0	40.0	26.5	41.0
58-59	35.36587396975136	38.0	34.0	40.0	26.0	41.0
60-61	35.00755514018618	38.0	33.0	40.0	26.0	41.0
62-63	34.908693383652164	37.0	33.5	40.0	26.0	41.0
64-65	34.65867751732395	37.0	33.0	39.5	26.0	41.0
66-67	34.33392264468065	36.0	33.0	39.0	26.0	41.0
68-69	33.94049168021154	36.0	32.5	39.0	25.5	41.0
70-71	33.503042281935855	35.0	32.0	38.5	24.5	40.0
72-73	33.18647522549463	35.0	31.5	37.5	24.5	40.0
74-75	32.924318306421206	35.0	31.0	37.0	24.0	39.5
76-77	32.683846061226035	35.0	31.0	36.5	24.0	39.0
78-79	32.293698289856955	35.0	31.0	36.0	23.5	39.0
80-81	31.91097062188691	35.0	31.0	36.0	22.5	37.5
82-83	31.611580126143735	34.5	30.5	35.0	21.5	37.0
84-85	31.484573678186578	34.0	30.5	35.0	22.0	37.0
86-87	31.47121422983261	34.0	31.0	35.0	23.0	36.0
88-89	31.25951717725269	34.0	30.5	35.0	21.0	36.0
90-91	31.02861445336496	34.0	30.0	35.0	20.0	36.0
92-93	30.73847733863252	34.0	30.0	35.0	20.0	35.5
94-95	30.669579118211757	34.0	29.5	35.0	20.0	35.0
96-97	30.60724975538175	34.0	29.0	35.0	19.0	35.0
98-99	30.612297719143648	34.0	30.0	35.0	19.0	35.0
100	29.259402504724804	33.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3807433842011605
1101	2	0.3774974117807268
1101	3	0.36602039419276977
1101	4	0.35415215687759627
1101	5	0.2570616040643898
1101	6	0.3813573566909696
1101	7	0.352406517433856
1101	8	0.408863232380817
1101	9	0.48472818753246116
1101	10-11	0.4976866739157728
1101	12-13	0.5757507311487089
1101	14-15	0.697482382821569
1101	16-17	0.6807932606844389
1101	18-19	0.6251889314131702
1101	20-21	0.6031264820433435
1101	22-23	0.6446891155016914
1101	24-25	0.700219510342265
1101	26-27	0.6718830366443385
1101	28-29	0.6971075326230505
1101	30-31	0.6947299262554196
1101	32-33	0.7270496215210116
1101	34-35	0.6449464434160461
1101	36-37	0.6425291180821802
1101	38-39	0.675752900608714
1101	40-41	0.6084600913841314
1101	42-43	0.6703659473482233
1101	44-45	0.5874119007188554
1101	46-47	0.6085695511936038
1101	48-49	0.6439504504092852
1101	50-51	0.6226541354285118
1101	52-53	0.6502626623259218
1101	54-55	0.6533906520431856
1101	56-57	0.6773136758367144
1101	58-59	0.7070915664319983
1101	60-61	0.7542635182514559
1101	62-63	0.6355457482285942
1101	64-65	0.685015773992383
1101	66-67	0.6574910725916965
1101	68-69	0.6654983523645868
1101	70-71	0.6421838194895457
1101	72-73	0.6409180301550279
1101	74-75	0.6454463911370283
1101	76-77	0.6219270273958344
1101	78-79	0.701394204926018
1101	80-81	0.6252355440791071
1101	82-83	0.6671015851282363
1101	84-85	0.5838343591973434
1101	86-87	0.48582853229710565
1101	88-89	0.5374910282008862
1101	90-91	0.5907567156978821
1101	92-93	0.5726647015293125
1101	94-95	0.5334849873282472
1101	96-97	0.5466310765773201
1101	98-99	0.6543023265565822
1101	100	0.5934627582306504
1102	1	0.27626372581891445
1102	2	0.34427850789943193
1102	3	0.36488900269667823
1102	4	0.38901327619458215
1102	5	0.3164364321655455
1102	6	0.36020576856637376
1102	7	0.35539017201853795
1102	8	0.37094742393504276
1102	9	0.4038275156923632
1102	10-11	0.4733883437708428
1102	12-13	0.5135754714074281
1102	14-15	0.615370524466897
1102	16-17	0.5947930080568042
1102	18-19	0.5478028396509345
1102	20-21	0.5625847383829736
1102	22-23	0.5692065254165115
1102	24-25	0.6655178791962655
1102	26-27	0.6235389360768124
1102	28-29	0.6232105204880938
1102	30-31	0.6432175899455643
1102	32-33	0.6379723032040729
1102	34-35	0.6612914173145867
1102	36-37	0.6948938349660523
1102	38-39	0.6311311867203813
1102	40-41	0.6264743770693073
1102	42-43	0.6608590669803149
1102	44-45	0.6294007864612112
1102	46-47	0.5523783066425878
1102	48-49	0.5243664099562722
1102	50-51	0.5728604819507765
1102	52-53	0.5088981131813597
1102	54-55	0.5449971328927248
1102	56-57	0.6173669721179991
1102	58-59	0.6234471683478944
1102	60-61	0.6709110341136935
1102	62-63	0.6370109205892263
1102	64-65	0.5885685877684637
1102	66-67	0.595720831991045
1102	68-69	0.6207965718151414
1102	70-71	0.5930164380632519
1102	72-73	0.5957587694847319
1102	74-75	0.597105380504253
1102	76-77	0.5914994551270141
1102	78-79	0.5472429285113165
1102	80-81	0.5513346610368721
1102	82-83	0.5810382435030164
1102	84-85	0.5763729477287249
1102	86-87	0.5453131379705027
1102	88-89	0.541775103899532
1102	90-91	0.6209875508019849
1102	92-93	0.5589783957806098
1102	94-95	0.5666743500750435
1102	96-97	0.6003224959982703
1102	98-99	0.550029014696598
1102	100	0.543882751670484
1103	1	0.24513973806043055
1103	2	0.31381651467271965
1103	3	0.2676480419073748
1103	4	0.372507458864618
1103	5	0.3941538724098521
1103	6	0.36722069199164764
1103	7	0.37875972092260923
1103	8	0.32820482855105837
1103	9	0.4986582813091047
1103	10-11	0.4672653348115716
1103	12-13	0.490063800858195
1103	14-15	0.5772449902056564
1103	16-17	0.5630294728950744
1103	18-19	0.5695154446271502
1103	20-21	0.5454233539693689
1103	22-23	0.5427175108062983
1103	24-25	0.6424721170705752
1103	26-27	0.6217577825293148
1103	28-29	0.6446329441254477
1103	30-31	0.6406073608412939
1103	32-33	0.6385582450373093
1103	34-35	0.5936696383796516
1103	36-37	0.6706457158918653
1103	38-39	0.6110519366110765
1103	40-41	0.5408464773766539
1103	42-43	0.6223687016272024
1103	44-45	0.6341507032632805
1103	46-47	0.5836525074530314
1103	48-49	0.5786215763847693
1103	50-51	0.689422775995105
1103	52-53	0.6162168232545113
1103	54-55	0.5299605910555769
1103	56-57	0.6429363396179468
1103	58-59	0.6522689551132217
1103	60-61	0.6465364541816854
1103	62-63	0.6001257206857957
1103	64-65	0.5031582751399952
1103	66-67	0.5238973943177143
1103	68-69	0.5355468801368986
1103	70-71	0.5451653264736152
1103	72-73	0.48179874364443265
1103	74-75	0.4691531915829543
1103	76-77	0.4395422808550862
1103	78-79	0.4213824008732914
1103	80-81	0.44622839606940445
1103	82-83	0.45834527323893326
1103	84-85	0.46958686755854373
1103	86-87	0.4471082883466835
1103	88-89	0.43189197252510425
1103	90-91	0.447930956631712
1103	92-93	0.4298153343576949
1103	94-95	0.43083235436870737
1103	96-97	0.4308818471196503
1103	98-99	0.39293108909838637
1103	100	0.3259048855832667
1104	1	0.26280307682284487
1104	2	0.24853028589691561
1104	3	0.22771136740790965
1104	4	0.28754436420376805
1104	5	0.3275405019432469
1104	6	0.2697698334918144
1104	7	0.32590481008806904
1104	8	0.3339357034624726
1104	9	0.4671114067623918
1104	10-11	0.4541232834466342
1104	12-13	0.4111306366138976
1104	14-15	0.5138465493060025
1104	16-17	0.5016584840972271
1104	18-19	0.45018626806587037
1104	20-21	0.5331219403353202
1104	22-23	0.5264412615989045
1104	24-25	0.5729546839083994
1104	26-27	0.5653003635407856
1104	28-29	0.5480066225993312
1104	30-31	0.5477207250913949
1104	32-33	0.5717885293804059
1104	34-35	0.565170220659283
1104	36-37	0.5832892654353685
1104	38-39	0.5522618971155282
1104	40-41	0.5379868005522113
1104	42-43	0.5621505021767419
1104	44-45	0.4819946523210703
1104	46-47	0.4911770523009906
1104	48-49	0.5575668835424565
1104	50-51	0.5536961490312038
1104	52-53	0.5842343579529867
1104	54-55	0.47328922056410505
1104	56-57	0.5504228394335229
1104	58-59	0.47808603227667845
1104	60-61	0.4878990714146809
1104	62-63	0.44678451310829814
1104	64-65	0.5146860235620352
1104	66-67	0.49122587048569244
1104	68-69	0.5395368839911328
1104	70-71	0.5291140734327087
1104	72-73	0.5794033985660363
1104	74-75	0.5744331416851054
1104	76-77	0.4926063772026765
1104	78-79	0.47344980440343676
1104	80-81	0.4026175577939419
1104	82-83	0.4324494743554155
1104	84-85	0.40025319438941054
1104	86-87	0.4130255134630332
1104	88-89	0.49604990499724266
1104	90-91	0.503046521525846
1104	92-93	0.42947558144318876
1104	94-95	0.4685450107657054
1104	96-97	0.46835193535262576
1104	98-99	0.551314050891726
1104	100	0.5305864552931183
1105	1	0.1760947466768208
1105	2	0.15765398207353343
1105	3	0.1423404236546304
1105	4	0.24217975570887518
1105	5	0.0992039795893831
1105	6	0.17988642996922266
1105	7	0.1829913407845467
1105	8	0.2413793240267026
1105	9	0.2611898397254464
1105	10-11	0.3249842522482993
1105	12-13	0.28349340562493097
1105	14-15	0.3865278781084811
1105	16-17	0.382500180159866
1105	18-19	0.28420147165920895
1105	20-21	0.35164309902019397
1105	22-23	0.3157305164564974
1105	24-25	0.3261184626590037
1105	26-27	0.3661604493080688
1105	28-29	0.3689358468792818
1105	30-31	0.3974245491950512
1105	32-33	0.43855545296558773
1105	34-35	0.37876191685027294
1105	36-37	0.40939386702000746
1105	38-39	0.3627869740088272
1105	40-41	0.36718600130340917
1105	42-43	0.3894785171102839
1105	44-45	0.36101239463827994
1105	46-47	0.4225108603508758
1105	48-49	0.3431517577793599
1105	50-51	0.38643364806218017
1105	52-53	0.37502348779960215
1105	54-55	0.3589557754824213
1105	56-57	0.39955358961744736
1105	58-59	0.41765965501254954
1105	60-61	0.4486585888055785
1105	62-63	0.4187681934005525
1105	64-65	0.5046812952208981
1105	66-67	0.49986009864431935
1105	68-69	0.3956791582872867
1105	70-71	0.37640777837292205
1105	72-73	0.37651854573758925
1105	74-75	0.38711420865762136
1105	76-77	0.39245731380953686
1105	78-79	0.35882398252687864
1105	80-81	0.28019125651185206
1105	82-83	0.3522557047791395
1105	84-85	0.3151513964389743
1105	86-87	0.32651419753129574
1105	88-89	0.3785575583898151
1105	90-91	0.3428755054869619
1105	92-93	0.45196639044131004
1105	94-95	0.3466301763526225
1105	96-97	0.3069825338291885
1105	98-99	0.3111245886750744
1105	100	0.4347210071454626
1106	1	0.177984046006614
1106	2	0.1716365661651622
1106	3	0.1887634567882337
1106	4	0.19537231794798515
1106	5	0.1366069447666618
1106	6	0.17863757448345297
1106	7	0.17152732499616974
1106	8	0.2024270558665009
1106	9	0.30985299760408935
1106	10-11	0.29079707447679226
1106	12-13	0.30186030640089
1106	14-15	0.35221977877341715
1106	16-17	0.32210547390352673
1106	18-19	0.35579091390358286
1106	20-21	0.40901139916659446
1106	22-23	0.39818747482270567
1106	24-25	0.413649185362992
1106	26-27	0.3930163295868354
1106	28-29	0.36459883229363754
1106	30-31	0.3882904302185679
1106	32-33	0.44037392654815477
1106	34-35	0.4174639971306888
1106	36-37	0.3868604867352303
1106	38-39	0.38755354479486215
1106	40-41	0.3495822768464478
1106	42-43	0.4324828406567889
1106	44-45	0.35627151950249925
1106	46-47	0.4123671445994077
1106	48-49	0.4008219614320936
1106	50-51	0.39548700402868775
1106	52-53	0.31285915706914835
1106	54-55	0.3461031273256623
1106	56-57	0.3610745517454177
1106	58-59	0.33341313394632266
1106	60-61	0.3242754089004265
1106	62-63	0.28842238284497057
1106	64-65	0.4309194235589331
1106	66-67	0.4407395096332749
1106	68-69	0.4140832418980338
1106	70-71	0.3401081041622547
1106	72-73	0.3274145901755716
1106	74-75	0.32923692409730165
1106	76-77	0.38717886187927775
1106	78-79	0.46711345600993326
1106	80-81	0.40123466072831704
1106	82-83	0.359368628192982
1106	84-85	0.3005128529053529
1106	86-87	0.35391529865281157
1106	88-89	0.44332486045646746
1106	90-91	0.33538769610593633
1106	92-93	0.37413833100380955
1106	94-95	0.3821727621827229
1106	96-97	0.27013428995463684
1106	98-99	0.39274257634369647
1106	100	0.5184646863365536
1107	1	0.10713540338704419
1107	2	0.10769385523432362
1107	3	0.13569295934080827
1107	4	0.11874702486354494
1107	5	0.08338569737597368
1107	6	0.14961534170085855
1107	7	0.17405318822289217
1107	8	0.1421744858373728
1107	9	0.25806301666337816
1107	10-11	0.23408666010841017
1107	12-13	0.27699958326292773
1107	14-15	0.2782108560823957
1107	16-17	0.289828562499288
1107	18-19	0.23299647900145004
1107	20-21	0.29821638761169567
1107	22-23	0.28014161113283365
1107	24-25	0.3773142369812845
1107	26-27	0.31677763548590576
1107	28-29	0.2823045821325607
1107	30-31	0.26080999824414874
1107	32-33	0.27189008201241194
1107	34-35	0.2840963959333962
1107	36-37	0.26552340528602514
1107	38-39	0.34385644551012007
1107	40-41	0.286017186116716
1107	42-43	0.2776758744251211
1107	44-45	0.2407608704932258
1107	46-47	0.2774426779997441
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2216	96-97	-1.4322152634363832
2216	98-99	-1.3692976596331725
2216	100	-1.4543050251705125
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1486.0
1	366.0
2	287.0
3	250.0
4	225.0
5	308.0
6	443.0
7	1132.0
8	3121.0
9	4557.0
10	5722.0
11	8218.0
12	11952.0
13	15183.0
14	17845.0
15	19056.0
16	20130.0
17	21768.0
18	23345.0
19	25868.0
20	28306.0
21	31730.0
22	35432.0
23	40071.0
24	47635.0
25	55456.0
26	61013.0
27	64087.0
28	71487.0
29	85517.0
30	101639.0
31	121922.0
32	154713.0
33	208960.0
34	302087.0
35	444372.0
36	704589.0
37	1082167.0
38	1002121.0
39	233057.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.163967597315406	19.202083397235658	13.40403321799582	22.229915787453113
2	13.66776048000383	23.583003300036225	37.17972229525522	25.56951392470473
3	23.1074909711399	24.902855539623406	30.30663795581819	21.683015533418505
4	11.41569840317896	16.785922537469983	42.18699761118913	29.611381448161932
5	11.801092177804282	40.77376082299371	34.99509986171183	12.430047137490183
6	28.066979158504125	38.32493135548479	17.799330849545626	15.808758636465456
7	25.253158445448054	33.22321712660218	23.464863333222524	18.058761094727245
8	24.182836853105147	30.902129592875795	20.944202220893366	23.970831333125695
9	23.380659217428345	19.430842948907554	23.76379173181634	33.42470610184776
10-11	25.099927157521883	24.762871170909943	28.23441922344952	21.902782448118653
12-13	28.351705074011246	23.446859848903017	26.73582877205311	21.465606305032622
14-15	23.274424864348077	25.519359286062514	28.495994944314653	22.710220905274756
16-17	20.95909778342738	28.37574641996905	28.429910993014207	22.235244803589367
18-19	20.385488598409175	31.849187983467132	26.661777521745666	21.103545896378026
20-21	23.11961576896756	27.265044587363356	25.097522801154387	24.517816842514698
22-23	21.660609070368363	27.56829882119242	26.172723603455307	24.598368504983913
24-25	24.75222782752908	27.469545257856975	25.150535036729305	22.62769187788464
26-27	23.470854148124648	25.130260306938602	29.516529019229488	21.882356525707262
28-29	21.134247447023675	30.98697210903911	26.243484620013085	21.635295823924125
30-31	22.627479054832303	26.706496172872995	26.0544510051959	24.6115737670988
32-33	23.106495443095255	27.667743533481232	26.890590852710126	22.335170170713383
34-35	24.912470492461633	26.70107328832037	26.58596680393895	21.800489415279046
36-37	23.384589447155836	25.510539467497868	29.402783296422175	21.70208778892412
38-39	22.5836322262776	28.191546884397862	27.803885596732332	21.42093529259221
40-41	22.2483572806497	28.769261029079303	26.825848894158554	22.156532796112444
42-43	21.631462470256402	28.45281249836125	24.58871984863256	25.327005182749783
44-45	23.02985420179042	26.192436618937936	25.822202566111812	24.95550661315983
46-47	24.8220708170812	28.093587346765613	26.49270949419989	20.591632341953297
48-49	23.66077595026737	27.192603510366176	27.011631298753162	22.13498924061329
50-51	19.740904646098496	28.012559092751687	29.973361449110257	22.27317481203956
52-53	22.323773548238933	29.402209249273405	25.12318765795176	23.150829544535902
54-55	21.10346927853693	26.630838341452883	27.03970985903258	25.22598252097761
56-57	23.984856733442136	25.101099243390777	26.776947215459185	24.137096807707902
58-59	26.231752109210827	25.814110405954622	25.946664917216268	22.007472567618287
60-61	23.127009981172808	26.98803409060662	29.30410433195625	20.580851596264328
62-63	21.314295501913556	27.41824132527308	28.338105885650904	22.929357287162453
64-65	20.358890427048834	30.66741408706029	26.18246623002275	22.791229255868124
66-67	22.83128735856254	26.592206411979873	25.021089760494537	25.55541646896305
68-69	21.49577219946558	26.90773487813731	26.569607807825165	25.02688511457195
70-71	24.36003697729055	26.709868190723178	25.57632543023901	23.353769401747257
72-73	23.208144858616876	24.64754036475096	29.774659558086263	22.3696552185459
74-75	21.104227091503365	30.187738792522513	26.451022361024112	22.257011754950014
76-77	22.06318460169101	26.199092895801762	26.295384277421295	25.44233822508593
78-79	22.756072239369182	26.744819733586723	27.324357229750802	23.174750797293296
80-81	24.302612459740033	25.73542596104407	27.006962099720827	22.954999479495076
82-83	22.78871260819406	24.8329131215524	29.790399458814466	22.587974811439075
84-85	21.976043306026934	27.477970560388094	27.970530701490503	22.575455432094465
86-87	21.56874328301526	28.316744527039674	27.04925859727045	23.065253592674622
88-89	21.315993756169	27.578625516093204	25.236179586828584	25.869201140909215
90-91	22.68705967754033	25.24567220129615	26.22606532827308	25.841202792890446
92-93	24.36812717088786	27.062432518435724	26.836567171267816	21.7328731394086
94-95	22.766247675256658	26.22307522728955	27.582221407784225	23.42845568966957
96-97	19.79841703138322	26.917155112936392	30.125242574064927	23.159185281615464
98-99	22.045302038826275	28.136160832460117	26.140173153430357	23.678363975283247
100	21.838022962899835	23.840310135902474	26.086000876022936	28.235666025174762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2293.0
1	1519.5
2	746.0
3	725.0
4	664.0
5	589.5
6	518.5
7	480.0
8	501.0
9	517.0
10	481.5
11	474.5
12	496.0
13	504.0
14	567.5
15	668.0
16	773.0
17	916.5
18	1164.0
19	1491.0
20	1965.0
21	2702.0
22	3944.5
23	5886.5
24	8349.0
25	11739.5
26	16446.0
27	22248.0
28	28781.0
29	36869.5
30	46128.0
31	55162.0
32	65841.0
33	75747.0
34	84245.0
35	93164.5
36	103815.5
37	117312.5
38	125804.0
39	128525.0
40	130197.0
41	130998.5
42	132989.5
43	141725.0
44	155755.0
45	170575.5
46	190015.0
47	286593.5
48	495659.0
49	509488.5
50	327787.0
51	221653.5
52	169279.5
53	145026.0
54	131610.5
55	116038.5
56	101735.5
57	88001.5
58	74537.5
59	64424.5
60	53128.0
61	41294.5
62	31940.5
63	24999.5
64	18340.0
65	12591.5
66	9237.5
67	6910.0
68	5318.0
69	4213.5
70	3265.0
71	2547.5
72	2131.5
73	1820.0
74	1570.5
75	1355.0
76	1078.5
77	749.0
78	472.5
79	294.0
80	195.5
81	134.5
82	99.0
83	84.5
84	64.0
85	47.5
86	48.0
87	35.5
88	24.5
89	25.5
90	25.0
91	19.5
92	16.0
93	16.0
94	12.5
95	13.5
96	13.0
97	9.0
98	7.0
99	7.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09086809278431027
2	0.06105323553480202
3	0.07532798814365146
4	0.07714693168937742
5	0.07793777670925829
6	0.08519377976666513
7	0.08163497717720128
8	0.08380980098187363
9	0.06908031248659272
10-11	0.07561466946335828
12-13	0.06680663305443524
14-15	0.07451737199827359
16-17	0.07591123634581359
18-19	0.07567398283984933
20-21	0.06765679145080716
22-23	0.07159124542471443
24-25	0.07398355160985401
26-27	0.06885294454337697
28-29	0.07667242467744892
30-31	0.08086390328281745
32-33	0.0825741056383098
34-35	0.08500595407444343
36-37	0.08511469526467705
38-39	0.08642947511022897
40-41	0.0833946073464362
42-43	0.08862407004039835
44-45	0.08543103327262938
46-47	0.08274216020503447
48-49	0.09132282867074175
50-51	0.0887822390443745
52-53	0.09325051340670135
54-55	0.08575725684333024
56-57	0.09248932507506602
58-59	0.09619641110575752
60-61	0.09524739708190051
62-63	0.09597892872529029
64-65	0.09778798670826774
66-67	0.10030880520913797
68-69	0.09685874380990775
70-71	0.09984418375995799
72-73	0.09740244976107583
74-75	0.1011490780427614
76-77	0.09862825954189117
78-79	0.1051329598304112
80-81	0.10073388440732395
82-83	0.10419383136930267
84-85	0.10077342665831797
86-87	0.09738267863557881
88-89	0.09675000261967413
90-91	0.09703668393938095
92-93	0.09191596243565239
94-95	0.0916391666786941
96-97	0.08951377068776431
98-99	0.10270111139427755
100	0.24419317101371107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5057881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.72816632382142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9807808405387	41.40065873964439
2	14.596982752605694	16.561201308315372
3	5.5428886881486745	9.43313734247179
4	2.669069829514403	6.05645748874347
5	1.44237784101205	4.091172503336302
6	0.8586188115867671	2.9224722451473606
7	0.5390670837061154	2.140620102892452
8	0.35513160064083876	1.6116771606398743
9	0.2432022309672451	1.241677494777088
>10	0.7621705920039624	6.312001292253975
>50	0.007259363875982021	0.271993484030615
>100	0.0017779491102420967	0.16567949791739683
>500	1.0344865990154857E-4	0.049148292073298544
>1k	2.068973198029721E-4	0.268129607740616
>5k	1.5517298985222907E-4	0.6141922684642782
>10k+	2.068973198029721E-4	6.859781171551753
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	144765	2.8621669825763	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	35775	0.7073120146559398	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	27770	0.5490441550522838	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	25475	0.5036694220366197	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	8068	0.1595134405099685	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	6646	0.1313989000532041	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	6218	0.12293685834047896	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.9771125497021382E-5	0.0
4	0.0	0.0	0.0	1.9771125497021382E-5	0.0
5	0.0	0.0	0.0	1.9771125497021382E-5	0.0
6	0.0	0.0	0.0	1.9771125497021382E-5	0.0
7	0.0	0.0	0.0	1.9771125497021382E-5	0.0
8	0.0	0.0	0.0	1.9771125497021382E-5	0.0
9	0.0	0.0	0.0	1.9771125497021382E-5	0.0
10-11	0.0	0.0	0.0	7.908450198808553E-5	0.0
12-13	0.0	0.0	0.0	7.908450198808553E-5	0.0
14-15	0.0	0.0	0.0	7.908450198808553E-5	0.0
16-17	0.0	0.0	0.0	8.897006473659622E-5	0.0
18-19	0.0	0.0	0.0	1.6805456672468172E-4	0.0
20-21	0.0	0.0	0.0	3.558802589463849E-4	0.0
22-23	0.0	0.0	0.0	7.611883316353233E-4	0.0
24-25	0.0	0.0	0.0	0.0011961530925697936	0.0
26-27	0.0	0.0	0.0	0.002234137181163416	0.0
28-29	0.0	0.0	0.0	0.005545800701914497	0.0
30-31	0.0	0.0	0.0	0.014393379361831566	0.0
32-33	0.0	9.885562748510691E-6	0.0	0.03866243590942531	0.0
34-35	9.885562748510691E-6	1.9771125497021382E-5	0.0	0.07461622762575869	0.0
36-37	1.9771125497021382E-5	1.9771125497021382E-5	0.0	0.12320376853468873	0.0
38-39	1.9771125497021382E-5	1.9771125497021382E-5	0.0	0.2017544501343547	0.0
40-41	1.9771125497021382E-5	1.9771125497021382E-5	0.0	0.28916259595668625	0.0
42-43	1.9771125497021382E-5	1.9771125497021382E-5	0.0	0.3776878103695994	0.0
44-45	1.9771125497021382E-5	1.9771125497021382E-5	0.0	0.4879019494527451	0.0
46-47	3.9542250994042765E-5	1.9771125497021382E-5	0.0	0.6279210602226506	0.0
48-49	3.9542250994042765E-5	1.9771125497021382E-5	0.0	0.7624536836671325	0.0
50-51	3.9542250994042765E-5	1.9771125497021382E-5	0.0	0.9077516849447427	0.0
52-53	3.9542250994042765E-5	1.9771125497021382E-5	0.0	1.0603155748425082	0.0
54-55	3.9542250994042765E-5	1.9771125497021382E-5	0.0	1.2361204227620224	0.0
56-57	3.9542250994042765E-5	1.9771125497021382E-5	0.0	1.4712781894235945	0.0
58-59	3.9542250994042765E-5	1.9771125497021382E-5	0.0	1.6964811943974167	0.0
60-61	3.9542250994042765E-5	1.9771125497021382E-5	0.0	1.9305713202821497	0.0
62-63	3.9542250994042765E-5	1.9771125497021382E-5	0.0	2.1743492976604237	0.0
64-65	3.9542250994042765E-5	1.9771125497021382E-5	0.0	2.430958735486264	0.0
66-67	3.9542250994042765E-5	1.9771125497021382E-5	0.0	2.733259244335721	0.0
68-69	3.9542250994042765E-5	1.9771125497021382E-5	0.0	3.075378799936179	0.0
70-71	3.9542250994042765E-5	1.9771125497021382E-5	0.0	3.4357471043703876	0.0
72-73	3.9542250994042765E-5	1.9771125497021382E-5	0.0	3.776808904756755	0.0
74-75	3.9542250994042765E-5	1.9771125497021382E-5	0.0	4.146894717372749	0.0
76-77	3.9542250994042765E-5	1.9771125497021382E-5	0.0	4.527221972996202	0.0
78-79	3.9542250994042765E-5	1.9771125497021382E-5	0.0	5.038157283652977	0.0
80-81	3.9542250994042765E-5	1.9771125497021382E-5	0.0	5.563120207849888	0.0
82-83	3.9542250994042765E-5	1.9771125497021382E-5	0.0	6.0441319200669215	0.0
84-85	3.9542250994042765E-5	1.9771125497021382E-5	0.0	6.536907451954683	0.0
86-87	3.9542250994042765E-5	1.9771125497021382E-5	0.0	7.030048749664138	0.0
88	3.9542250994042765E-5	1.9771125497021382E-5	0.0	7.446082657935211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1750	0.0	28.21295	1
GAGTACG	2620	0.0	26.202868	1
GTACGCA	2615	0.0	25.346365	3
AGTACGC	2890	0.0	22.934515	2
CGCAGTG	2955	0.0	22.741209	6
TACGCAG	3015	0.0	22.139137	4
GCATCGT	1795	0.0	21.990595	8
TACGGGC	1240	0.0	20.849743	4
GGCGTCG	1200	0.0	20.754715	8
ATACGGG	1295	0.0	19.964626	3
TAACGCC	755	0.0	19.923367	4
CATGGGG	7935	0.0	19.904535	4
CTCGTCG	1150	0.0	19.613195	9
CGGGCGT	1310	0.0	19.371244	6
ACGGGCG	1290	0.0	19.312635	5
GCTATTG	2695	0.0	19.180328	8
CTAACGC	770	0.0	18.925146	3
TGATCGC	1235	0.0	18.643785	9
TAGGCAT	2955	0.0	18.6116	5
GGGCTAT	2805	0.0	18.428707	6
>>END_MODULE