FastQCFastQC Report
Mon 16 Jan 2017
ERR1146607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057881
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1527313.0196637682855725No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC394240.7794568515945709No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT348420.688865554567219No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA289020.571425069114912No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99780.19727629020927936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99190.1961097938049551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97420.1926103045919823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG27000.024.7334421
GTACGCA27150.023.5560933
CGCAGTG28650.022.4740356
TACGCAG28550.022.400984
GAGTACT126300.022.25205412-13
GTACTAG17100.022.001581
AGTACGC30750.021.1047922
GTACTTT140550.020.09638814-15
CATGGGG80650.018.5420174
GTGATCG12450.018.1194138
AGTACTT136550.018.06923912-13
GTACCCG5400.017.4179171
GGGCTAT25150.017.3792116
GTATAAG25050.017.2718981
TGATCGC13100.017.2203589
ACTTTTT165750.017.02691716-17
CTATTGA25250.016.9376149
GCTATTG25650.016.8567078
TAACGCC7550.016.8170974
CTAACGC7650.016.5972653
Produced by FastQC (version 0.11.5)