##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5057881
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25479820501906	33.0	31.0	34.0	30.0	34.0
2	32.276091904890606	33.0	31.0	34.0	30.0	34.0
3	32.432003837180034	34.0	31.0	34.0	30.0	34.0
4	35.91461641742856	37.0	35.0	37.0	35.0	37.0
5	35.98957547637044	37.0	35.0	37.0	35.0	37.0
6	36.10854782862626	37.0	35.0	37.0	35.0	37.0
7	36.09096675070054	37.0	36.0	37.0	35.0	37.0
8	36.09314987837792	37.0	36.0	37.0	35.0	37.0
9	37.86359208530212	39.0	38.0	39.0	35.0	39.0
10-11	37.81749363023764	39.0	38.0	39.0	35.0	39.0
12-13	37.81662152589197	39.0	38.0	39.0	35.0	39.0
14-15	39.07925502794549	40.0	38.0	41.0	36.0	41.0
16-17	39.08555954558836	40.0	38.5	41.0	36.0	41.0
18-19	39.20953913308755	40.0	39.0	41.0	36.0	41.0
20-21	39.259578566597355	40.0	39.0	41.0	36.0	41.0
22-23	39.220468314695424	40.0	39.0	41.0	36.0	41.0
24-25	39.186725330232164	40.5	39.0	41.0	36.0	41.0
26-27	39.066408442586926	40.0	39.0	41.0	35.5	41.0
28-29	39.04496675584103	40.0	39.0	41.0	35.0	41.0
30-31	38.98505115086733	40.0	39.0	41.0	35.0	41.0
32-33	38.88406617316619	40.0	38.0	41.0	35.0	41.0
34-35	38.775706367943414	40.0	38.0	41.0	35.0	41.0
36-37	38.68928855779723	40.0	38.0	41.0	35.0	41.0
38-39	38.615296306892155	40.0	38.0	41.0	35.0	41.0
40-41	38.627099174535736	40.0	38.0	41.0	35.0	41.0
42-43	38.54628460021104	40.0	38.0	41.0	35.0	41.0
44-45	38.42241246877892	40.0	38.0	41.0	34.0	41.0
46-47	38.21505913247069	40.0	38.0	41.0	33.5	41.0
48-49	37.93558112577184	40.0	38.0	41.0	33.0	41.0
50-51	37.793825220482645	40.0	37.0	41.0	33.0	41.0
52-53	37.816722358632006	40.0	37.0	41.0	33.0	41.0
54-55	37.68605924101417	40.0	37.0	41.0	33.0	41.0
56-57	37.47477698664717	40.0	36.5	41.0	32.5	41.0
58-59	37.29654691361857	39.0	36.0	41.0	32.0	41.0
60-61	36.96609993394467	39.0	35.0	41.0	31.0	41.0
62-63	36.83196905977029	39.0	35.0	41.0	31.0	41.0
64-65	36.57432677834848	38.5	35.0	40.5	31.0	41.0
66-67	36.235532132922856	37.5	35.0	40.0	31.0	41.0
68-69	35.8320787895168	37.0	35.0	39.0	31.0	41.0
70-71	35.37974420513254	36.0	34.5	39.0	30.0	41.0
72-73	35.00203919388376	36.0	34.0	39.0	29.5	41.0
74-75	34.731082937696634	35.0	34.0	37.5	30.0	40.0
76-77	34.44556752126039	35.0	34.0	37.0	30.0	39.0
78-79	34.12852556238472	35.0	34.0	36.5	29.0	39.0
80-81	33.81643913725926	35.0	34.0	36.0	29.0	39.0
82-83	33.510774175983975	35.0	34.0	36.0	29.0	37.0
84-85	33.32820967911266	35.0	34.0	35.0	29.0	37.0
86-87	33.27299199012393	35.0	34.0	35.0	29.0	36.5
88-89	33.06970814457675	35.0	34.0	35.0	29.0	36.0
90-91	32.93424617937828	35.0	33.5	35.0	29.0	36.0
92-93	32.80111424131964	35.0	33.0	35.0	28.5	36.0
94-95	32.68536211112915	35.0	33.0	35.0	27.5	35.5
96-97	32.616873054150545	35.0	33.0	35.0	27.0	35.0
98-99	32.63338777642258	35.0	33.0	35.0	27.0	35.0
100	31.792506980690135	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30331650545637956
1101	2	0.2931921992613482
1101	3	0.3033015527399243
1101	4	0.19247952870389184
1101	5	0.19072740977970426
1101	6	0.223048931364346
1101	7	0.2195473750621133
1101	8	0.2379367506318033
1101	9	0.27640410717064157
1101	10-11	0.2802740620240556
1101	12-13	0.33280486894784644
1101	14-15	0.46669201789417514
1101	16-17	0.513984677425384
1101	18-19	0.4517209421670003
1101	20-21	0.4359283906764446
1101	22-23	0.3824052090378558
1101	24-25	0.4164614773333142
1101	26-27	0.42712800203377554
1101	28-29	0.4599416213420042
1101	30-31	0.5052746765482183
1101	32-33	0.4315051396592864
1101	34-35	0.4188818350219776
1101	36-37	0.44800516899392306
1101	38-39	0.4934387965380651
1101	40-41	0.4367911212872855
1101	42-43	0.4510217037366786
1101	44-45	0.4190472441066504
1101	46-47	0.4420481251184327
1101	48-49	0.5105955855976063
1101	50-51	0.43981217183085164
1101	52-53	0.39740850380038495
1101	54-55	0.41708060588636187
1101	56-57	0.36628988364719106
1101	58-59	0.4189973886039198
1101	60-61	0.3680963723477859
1101	62-63	0.33366117517581273
1101	64-65	0.3244160523951507
1101	66-67	0.31196715200800185
1101	68-69	0.2610997403302946
1101	70-71	0.30576472427495816
1101	72-73	0.2809358351410296
1101	74-75	0.2790369076222703
1101	76-77	0.2979386346508832
1101	78-79	0.3103232996538239
1101	80-81	0.3073534967803866
1101	82-83	0.3141005820781757
1101	84-85	0.31934631035505845
1101	86-87	0.29366437428268455
1101	88-89	0.4297784500420434
1101	90-91	0.3894759619866264
1101	92-93	0.39677467141070366
1101	94-95	0.42185722564554595
1101	96-97	0.3428776038412096
1101	98-99	0.3735152918753002
1101	100	0.43618582692544194
1102	1	0.2467266253662146
1102	2	0.22886444343002665
1102	3	0.22842734024884948
1102	4	0.2024071155735001
1102	5	0.17927590783371983
1102	6	0.23066504330283522
1102	7	0.2024902560901154
1102	8	0.23730280467646025
1102	9	0.2635018978333292
1102	10-11	0.27956259605713996
1102	12-13	0.27331656214381894
1102	14-15	0.45497162371357547
1102	16-17	0.5135390477136497
1102	18-19	0.39006760975725285
1102	20-21	0.3721211700082421
1102	22-23	0.3784964415686858
1102	24-25	0.39661295883818326
1102	26-27	0.3815118384831848
1102	28-29	0.350750152006718
1102	30-31	0.43729584022443646
1102	32-33	0.4058316866809619
1102	34-35	0.42081786590231474
1102	36-37	0.44347807501983993
1102	38-39	0.42179760655324827
1102	40-41	0.4264609940159261
1102	42-43	0.4192825730066332
1102	44-45	0.39931841813924507
1102	46-47	0.40912103331748995
1102	48-49	0.4939544533368476
1102	50-51	0.5008485359487622
1102	52-53	0.4190975368409582
1102	54-55	0.45604617722209184
1102	56-57	0.38421350860924974
1102	58-59	0.345509062784906
1102	60-61	0.4197773647669152
1102	62-63	0.4154501523259171
1102	64-65	0.4070788698859431
1102	66-67	0.3767929645759551
1102	68-69	0.4189781232146288
1102	70-71	0.4208510760670876
1102	72-73	0.40229818398040607
1102	74-75	0.3506598296411454
1102	76-77	0.30207205056357367
1102	78-79	0.3937308593320097
1102	80-81	0.42538489595037987
1102	82-83	0.4210821708207604
1102	84-85	0.40327494848158807
1102	86-87	0.3679882727888497
1102	88-89	0.4041213974866622
1102	90-91	0.3876574165377562
1102	92-93	0.4161281212379677
1102	94-95	0.4272390091558904
1102	96-97	0.44874148508449707
1102	98-99	0.45744834419434
1102	100	0.509689127043675
1103	1	0.2083988059350972
1103	2	0.15007724090342123
1103	3	0.19277692817438208
1103	4	0.162983824067517
1103	5	0.09789312556434027
1103	6	0.1373856091792902
1103	7	0.13619240096545582
1103	8	0.1033233333585386
1103	9	0.17891968196242658
1103	10-11	0.20967926892286215
1103	12-13	0.19861049554631904
1103	14-15	0.3027609404464755
1103	16-17	0.38392893606528844
1103	18-19	0.3323842643520578
1103	20-21	0.30817771850122
1103	22-23	0.3337692329082671
1103	24-25	0.31750810334157364
1103	26-27	0.3571853828456284
1103	28-29	0.34015442218140635
1103	30-31	0.33221316431790626
1103	32-33	0.40940237882251296
1103	34-35	0.42799882630009023
1103	36-37	0.4167368891067156
1103	38-39	0.41161966085703483
1103	40-41	0.3888706019089696
1103	42-43	0.38277394978560153
1103	44-45	0.399221353884343
1103	46-47	0.46904563315174386
1103	48-49	0.5188935067727627
1103	50-51	0.45741660490843117
1103	52-53	0.4365028706441123
1103	54-55	0.49727143807208307
1103	56-57	0.4666951561240751
1103	58-59	0.39115490712972445
1103	60-61	0.4263116651538681
1103	62-63	0.3805570371995515
1103	64-65	0.3545993431027341
1103	66-67	0.36437288353137376
1103	68-69	0.33978129321900497
1103	70-71	0.2990760889546067
1103	72-73	0.2911974916430964
1103	74-75	0.3107248871619106
1103	76-77	0.29385016983352585
1103	78-79	0.3431890613431179
1103	80-81	0.3488342496166368
1103	82-83	0.27134892623188733
1103	84-85	0.22511029487863254
1103	86-87	0.2584840345882782
1103	88-89	0.3625746989763954
1103	90-91	0.2524532063960976
1103	92-93	0.32233044555983525
1103	94-95	0.3395554353642183
1103	96-97	0.31753364686042573
1103	98-99	0.3703022943016876
1103	100	0.31452211127195895
1104	1	0.22450329301097582
1104	2	0.20020269004616154
1104	3	0.18527041120124466
1104	4	0.153518402360298
1104	5	0.10172170741297037
1104	6	0.14753658745051723
1104	7	0.12024937733857399
1104	8	0.14316924683302545
1104	9	0.2195279051599499
1104	10-11	0.1987386282909327
1104	12-13	0.23933343404847562
1104	14-15	0.36637942502746057
1104	16-17	0.3670349109588855
1104	18-19	0.32261980786140043
1104	20-21	0.3337208962975069
1104	22-23	0.36187220315606083
1104	24-25	0.3572841432598466
1104	26-27	0.35079702926719847
1104	28-29	0.3517057104491954
1104	30-31	0.32992633875728217
1104	32-33	0.34485663982469816
1104	34-35	0.3740122829689554
1104	36-37	0.3776647651744014
1104	38-39	0.39350608110130025
1104	40-41	0.3870039087185262
1104	42-43	0.3649807274376613
1104	44-45	0.3417885854244247
1104	46-47	0.42839300336140695
1104	48-49	0.4704712773670181
1104	50-51	0.4232674584350633
1104	52-53	0.3938951281899179
1104	54-55	0.3707246256904497
1104	56-57	0.3837799451564905
1104	58-59	0.38054846599798964
1104	60-61	0.43122218887399555
1104	62-63	0.39440988415325506
1104	64-65	0.4150208845481984
1104	66-67	0.30556390440641223
1104	68-69	0.31196799154842125
1104	70-71	0.2609865670870022
1104	72-73	0.34208993361198736
1104	74-75	0.315773438093764
1104	76-77	0.26994236894415025
1104	78-79	0.289626007296782
1104	80-81	0.26234881489619255
1104	82-83	0.33314015100840777
1104	84-85	0.29178290867182
1104	86-87	0.26111322399700754
1104	88-89	0.3336966544413329
1104	90-91	0.29845172272271725
1104	92-93	0.32639417720559294
1104	94-95	0.34683886256682683
1104	96-97	0.3313116677727592
1104	98-99	0.2808190312148966
1104	100	0.37914085280196375
1105	1	0.15266485134078778
1105	2	0.18516979958195634
1105	3	0.1561141043591121
1105	4	0.1482412450739048
1105	5	0.10570177958562965
1105	6	0.1267408707834008
1105	7	0.15060445470933104
1105	8	0.14267176989844899
1105	9	0.20086584601615698
1105	10-11	0.18563853069510117
1105	12-13	0.19616113812310942
1105	14-15	0.2574319763551145
1105	16-17	0.31094181962488676
1105	18-19	0.2656531798873871
1105	20-21	0.25448421792378895
1105	22-23	0.26199677774654617
1105	24-25	0.2939366933553629
1105	26-27	0.2914207955218302
1105	28-29	0.2489477168060148
1105	30-31	0.2876663405746882
1105	32-33	0.26822401447616784
1105	34-35	0.24446369323135997
1105	36-37	0.22868287302678425
1105	38-39	0.24727763907687006
1105	40-41	0.22613082899746217
1105	42-43	0.24471773207205416
1105	44-45	0.2367775705971411
1105	46-47	0.22928556117834376
1105	48-49	0.3438364444325046
1105	50-51	0.32850875528400536
1105	52-53	0.2773831037770975
1105	54-55	0.2873750349409576
1105	56-57	0.22966361872332186
1105	58-59	0.2433736934691879
1105	60-61	0.23245307207780996
1105	62-63	0.25033948577639364
1105	64-65	0.24998098628196885
1105	66-67	0.2555184435369
1105	68-69	0.27334306605445136
1105	70-71	0.24380166533227765
1105	72-73	0.2896903133167825
1105	74-75	0.31254381680771104
1105	76-77	0.2559388296190832
1105	78-79	0.2836420374513864
1105	80-81	0.24998992712935575
1105	82-83	0.25229552861085836
1105	84-85	0.22345837915717226
1105	86-87	0.2687410753124908
1105	88-89	0.3507995418097849
1105	90-91	0.29400125504819385
1105	92-93	0.29326768098851375
1105	94-95	0.296436080803133
1105	96-97	0.3448976909811634
1105	98-99	0.3152413290654792
1105	100	0.263046216157786
1106	1	0.12497303068355592
1106	2	0.10045976708892823
1106	3	0.11886907977913097
1106	4	0.10341272400869173
1106	5	0.10415251300293704
1106	6	0.1272789571810904
1106	7	0.15375682381464628
1106	8	0.16191729942242006
1106	9	0.16580767444483513
1106	10-11	0.1532197943902318
1106	12-13	0.1697303033282438
1106	14-15	0.2892724040083152
1106	16-17	0.3290594243266938
1106	18-19	0.2667230372428975
1106	20-21	0.2478031377367813
1106	22-23	0.28522519312004846
1106	24-25	0.2642608326701037
1106	26-27	0.28350810445793684
1106	28-29	0.2813343936084465
1106	30-31	0.3096552735216136
1106	32-33	0.2904087351593674
1106	34-35	0.3032885667751444
1106	36-37	0.295736682831361
1106	38-39	0.31273981243122506
1106	40-41	0.2997793594437397
1106	42-43	0.31088313056685024
1106	44-45	0.28903880019544914
1106	46-47	0.3368712999177319
1106	48-49	0.39970115118460825
1106	50-51	0.3432941919451764
1106	52-53	0.3254764979580287
1106	54-55	0.334856450257611
1106	56-57	0.3189094838574178
1106	58-59	0.33957453231976586
1106	60-61	0.36326112005215094
1106	62-63	0.31509971709839846
1106	64-65	0.3668215016597216
1106	66-67	0.3296970796304848
1106	68-69	0.2708860505398718
1106	70-71	0.30188861521651944
1106	72-73	0.2745024812147321
1106	74-75	0.2964932628580854
1106	76-77	0.288638584798413
1106	78-79	0.24306518153289147
1106	80-81	0.2625083574757312
1106	82-83	0.27112385690045215
1106	84-85	0.21795185248675608
1106	86-87	0.26444714949893466
1106	88-89	0.28874668678323445
1106	90-91	0.26167826044788
1106	92-93	0.27802078041349176
1106	94-95	0.27831220616173624
1106	96-97	0.3226860614280511
1106	98-99	0.27949961577800053
1106	100	0.3528400503519684
1107	1	0.11261110199671975
1107	2	0.1016401823052675
1107	3	0.10209336886236287
1107	4	0.06390001466352402
1107	5	0.09921161731149652
1107	6	0.10917046908394212
1107	7	0.0899853164240696
1107	8	0.12246425217967527
1107	9	0.12903285239286788
1107	10-11	0.13463576016297196
1107	12-13	0.13888786508199047
1107	14-15	0.2501707252949359
1107	16-17	0.2710819096245416
1107	18-19	0.2149856116463198
1107	20-21	0.16685785429811517
1107	22-23	0.14805821494625349
1107	24-25	0.1825629348630713
1107	26-27	0.1516462173369746
1107	28-29	0.1513469053163945
1107	30-31	0.1950601049801577
1107	32-33	0.16790290397753438
1107	34-35	0.16064836204780875
1107	36-37	0.13690947544277776
1107	38-39	0.1809149237871921
1107	40-41	0.19531651284800944
1107	42-43	0.19768898141879987
1107	44-45	0.22926198429911437
1107	46-47	0.2524176525872548
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2216	70-71	-1.1341934320299671
2216	72-73	-1.0860433560659004
2216	74-75	-1.0570486082716997
2216	76-77	-1.0565223290606554
2216	78-79	-1.0455295203444948
2216	80-81	-1.1431748636453989
2216	82-83	-1.1750970967171313
2216	84-85	-1.132669054898905
2216	86-87	-1.0991466473777365
2216	88-89	-1.007196591320998
2216	90-91	-1.0686989935362519
2216	92-93	-1.1057362549319336
2216	94-95	-1.1347370996967996
2216	96-97	-1.171066091645546
2216	98-99	-1.1287451714178793
2216	100	-1.3800538390832031
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	45.0
8	162.0
9	301.0
10	298.0
11	212.0
12	220.0
13	278.0
14	501.0
15	843.0
16	1596.0
17	3036.0
18	5070.0
19	7599.0
20	10855.0
21	16076.0
22	23265.0
23	27717.0
24	28101.0
25	27691.0
26	29270.0
27	34710.0
28	43719.0
29	53552.0
30	67547.0
31	85760.0
32	109577.0
33	143741.0
34	206675.0
35	318218.0
36	586105.0
37	1229210.0
38	1534192.0
39	460589.0
40	1149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7565357202737	19.418578718100402	13.720148746160344	22.10473681546555
2	14.06957151686675	23.711559123095824	36.78953406112104	25.429335298916385
3	23.018881055558126	24.965192885390188	30.698688816684637	21.31723724236705
4	11.672388299582128	17.17453205746359	41.5687263556116	29.58435328734268
5	11.604484650002986	40.602053942179445	35.263393903973004	12.530067503844567
6	28.014663764916303	38.65850791585082	17.29396554161558	16.032862777617293
7	24.929085887582524	33.53640923398919	23.504318707505657	18.030186170922633
8	24.381661281326693	30.7962625351754	20.732573632323664	24.08950255117424
9	23.390491668446067	18.89544631347521	23.822055881120153	33.89200613695857
10-11	25.20807892403067	24.563767027526275	28.23491241828463	21.993241630158426
12-13	28.790426267442825	23.19987362296582	26.95530005549755	21.0544000540938
14-15	23.40127681953704	25.187005227486615	29.10729396506046	22.304423987915886
16-17	20.667900388800412	28.052231524566196	29.014139365127377	22.26572872150601
18-19	20.020000506141713	30.96131139736237	28.094018591217807	20.92466950527811
20-21	22.710349499037935	26.417603227288648	26.837206902196737	24.034840371476676
22-23	21.26486368500959	26.780562848354872	27.806605177148295	24.14796828948724
24-25	24.254920192863374	26.73639415399453	26.923686025827813	22.084999627314282
26-27	23.14401579004913	24.361485044393202	31.048026352600278	21.446472812957392
28-29	20.731705629294165	30.14177280960149	27.954087887793328	21.172433673311016
30-31	22.334347791788282	25.90170844610311	27.66893422153313	24.09500954057547
32-33	22.765090018597746	26.815301654065347	28.52067317249954	21.898935154837364
34-35	24.517465052496387	25.88028127244027	28.27924471002825	21.323008965035097
36-37	23.025565266698802	24.749252552157248	30.896366025435963	21.328816155707987
38-39	22.172463466675946	27.375832567526743	29.395871524806598	21.055832440990713
40-41	21.992042073212925	27.924068527140978	28.219361981869767	21.864527417776326
42-43	21.44117269662928	27.71255393315897	25.992426472667113	24.853846897544642
44-45	22.895704643290628	25.44224757639451	27.08215159107899	24.579896189235875
46-47	24.714331950474914	27.477524678813126	27.473787936094183	20.33435543461778
48-49	23.691713675859194	26.746375355979467	27.764876007240986	21.797034960920357
50-51	19.75002970611606	27.64978060970592	30.568779692523414	22.03140999165461
52-53	22.28337450683243	29.024262213404274	25.758556228764505	22.93380705099879
54-55	21.16656777773538	26.323983073483802	27.53448442553703	24.97496472324379
56-57	23.913312967723783	24.803281932939004	27.420034073611575	23.863371025725634
58-59	26.102108202107203	25.44879578547406	26.546724832465557	21.902371179953185
60-61	22.95105708905678	26.737793039416736	29.89858145315442	20.412568418372068
62-63	21.177999480611557	27.103713488964676	28.90245432293011	22.815832707493655
64-65	20.256802080878412	30.32685339107576	26.745212999005112	22.671131529040718
66-67	22.734753026074515	26.25768572954635	25.682706116247644	25.32485512813149
68-69	21.450876602956683	26.60468669647036	27.083327154712467	24.86110954586049
70-71	24.223342074109592	26.4253849937101	26.15053742180877	23.200735510371537
72-73	23.19804133842797	24.278533918166406	30.21220250667319	22.311222236732437
74-75	21.106910511880393	29.85037400074025	26.80510322388796	22.237612263491393
76-77	22.1338755860905	25.901008488895865	26.54779892543319	25.417316999580443
78-79	22.726786980184226	26.518791173300478	27.584182374458845	23.17023947205645
80-81	24.225278011985573	25.530936633799804	27.294093781909062	22.949691572305557
82-83	22.758806539502533	24.649789193879254	29.948694567935185	22.642709698683024
84-85	21.885235003160926	27.24369953087844	28.15031858845371	22.72074687750693
86-87	21.570189176060094	28.064025231119516	27.07391692291693	23.29186866990346
88-89	21.243352700468833	27.445940305831634	25.345248336210364	25.965458657489176
90-91	22.640928610405943	24.98272991846386	26.299546450291427	26.076795020838773
92-93	24.317952675217487	26.993362928745718	26.76558956487389	21.923094831162906
94-95	22.684821844736256	26.147252478930145	27.601736103731263	23.56618957260234
96-97	19.674032261652034	26.76076109225982	30.136011012557184	23.429195633530963
98-99	21.93790774885315	27.94201718013139	26.054595035170387	24.065480035845074
100	21.709752871127595	23.659721669885716	26.08356551251749	28.546959946469197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	185.0
2	221.5
3	410.0
4	309.5
5	227.0
6	414.0
7	964.0
8	1921.0
9	2471.5
10	1983.5
11	1412.5
12	1428.5
13	1630.5
14	1891.5
15	2233.0
16	2671.5
17	3155.0
18	3741.5
19	4362.5
20	4955.0
21	5814.5
22	7041.5
23	8902.5
24	11277.5
25	14437.5
26	19111.5
27	24842.5
28	30862.5
29	38221.0
30	47073.5
31	55991.0
32	66161.5
33	74888.0
34	82493.5
35	91279.5
36	101584.0
37	114684.5
38	122974.5
39	125170.0
40	126870.5
41	128290.5
42	129845.5
43	137766.5
44	151618.5
45	166424.5
46	181941.0
47	288942.0
48	517445.0
49	514973.0
50	314713.5
51	214690.0
52	168005.0
53	146498.0
54	133099.0
55	116330.0
56	101718.0
57	88156.5
58	74629.0
59	64732.0
60	53007.0
61	40288.5
62	30655.5
63	23824.5
64	16949.5
65	11245.5
66	8296.5
67	6333.0
68	4771.0
69	3589.0
70	2674.0
71	2035.5
72	1715.5
73	1562.5
74	1341.5
75	1137.5
76	871.0
77	557.0
78	337.0
79	188.5
80	119.0
81	62.5
82	35.0
83	15.5
84	7.5
85	7.0
86	5.5
87	4.5
88	3.0
89	2.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0027086441930919292
2	0.0035390314639668274
3	1.3839787847914968E-4
4	0.04954644049553558
5	0.0018782569222170313
6	0.004824154621273217
7	3.9542250994042765E-5
8	2.7679575695829935E-4
9	1.9771125497021382E-5
10-11	8.897006473659621E-5
12-13	0.0
14-15	1.9771125497021382E-5
16-17	3.163380079523421E-4
18-19	1.7794012947319241E-4
20-21	2.5702463146127797E-4
22-23	0.0
24-25	0.0
26-27	2.372535059642566E-4
28-29	0.0
30-31	1.977112549702138E-4
32-33	1.878256922217031E-4
34-35	1.7794012947319241E-4
36-37	5.931337649106415E-5
38-39	6.524471414017056E-4
40-41	6.129048904076628E-4
42-43	0.0
44-45	4.745070119285132E-4
46-47	0.0
48-49	9.88556274851069E-5
50-51	0.0
52-53	3.163380079523421E-4
54-55	3.5588025894638483E-4
56-57	1.5816900397617106E-4
58-59	8.00730582629366E-4
60-61	5.7336263941362E-4
62-63	1.878256922217031E-4
64-65	3.3610913344936345E-4
66-67	9.786707121025584E-4
68-69	0.002332992808648523
70-71	0.0033314346462481026
72-73	0.0027976142578285254
74-75	0.0027086441930919292
76-77	0.004250791981859597
78-79	0.0013345509710489432
80-81	9.984418375995796E-4
82-83	6.821038296472376E-4
84-85	4.646214491800025E-4
86-87	0.0
88-89	0.0
90-91	1.9771125497021382E-5
92-93	4.942781374255345E-5
94-95	6.919893923957484E-5
96-97	3.6576582169489555E-4
98-99	6.919893923957484E-5
100	0.07107719616179187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5057881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.092245875448754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.22405371338117	37.28352726121553
2	15.778611704976072	16.75443864427647
3	6.19213293852916	9.862627333975654
4	3.0190848370144074	6.411599779424322
5	1.6266132748376123	4.3180275965973705
6	0.9681265019303674	3.0840006167415113
7	0.5900554972817516	2.1929160079290053
8	0.40243495120612305	1.7092940302647766
9	0.2803289259640635	1.3394963036956076
>10	0.9013509377824348	6.9725240013434835
>50	0.010304669517116644	0.36500478232801775
>100	0.005398014465197373	0.5401869211477474
>500	7.241640930864167E-4	0.27268148622996496
>1k	3.89934510695885E-4	0.44888932652155783
>5k	1.671147902982364E-4	0.8765733791522238
>10k+	2.2281972039764857E-4	7.568212529156683
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	152731	3.0196637682855725	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	39424	0.7794568515945709	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	34842	0.688865554567219	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	28902	0.571425069114912	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9978	0.19727629020927936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9919	0.1961097938049551	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9742	0.1926103045919823	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.9771125497021382E-5	0.0
4	0.0	0.0	0.0	1.9771125497021382E-5	0.0
5	0.0	0.0	0.0	1.9771125497021382E-5	0.0
6	0.0	0.0	0.0	1.9771125497021382E-5	0.0
7	0.0	0.0	0.0	1.9771125497021382E-5	0.0
8	0.0	0.0	0.0	1.9771125497021382E-5	0.0
9	0.0	0.0	0.0	1.9771125497021382E-5	0.0
10-11	0.0	0.0	0.0	7.908450198808553E-5	0.0
12-13	0.0	0.0	0.0	7.908450198808553E-5	0.0
14-15	0.0	0.0	0.0	7.908450198808553E-5	0.0
16-17	0.0	0.0	0.0	8.897006473659622E-5	0.0
18-19	0.0	0.0	0.0	1.6805456672468172E-4	0.0
20-21	0.0	0.0	0.0	3.756513844434062E-4	0.0
22-23	0.0	0.0	0.0	8.007305826293659E-4	0.0
24-25	0.0	0.0	0.0	0.0012356953435638364	0.0
26-27	0.0	0.0	0.0	0.002244022743911927	0.0
28-29	0.0	0.0	0.0	0.005526029576417476	0.0
30-31	0.0	0.0	0.0	0.014383493799083054	0.0
32-33	0.0	0.0	0.0	0.03881071935065297	0.0
34-35	0.0	0.0	0.0	0.0750907346376872	0.0
36-37	0.0	0.0	0.0	0.12396495686632406	0.0
38-39	0.0	0.0	0.0	0.20288140428768492	0.0
40-41	0.0	1.9771125497021382E-5	0.0	0.29099142506516074	0.0
42-43	0.0	1.9771125497021382E-5	0.0	0.3799713753645054	0.0
44-45	0.0	1.9771125497021382E-5	0.0	0.490571051394843	0.0
46-47	0.0	1.9771125497021382E-5	0.0	0.6310547836139284	0.0
48-49	0.0	1.9771125497021382E-5	0.0	0.7664079087665367	0.0
50-51	0.0	1.9771125497021382E-5	0.0	0.9128427497602256	0.0
52-53	0.0	1.9771125497021382E-5	0.0	1.0669290163212617	0.0
54-55	0.0	1.9771125497021382E-5	0.0	1.2440980719000705	0.0
56-57	0.0	1.9771125497021382E-5	0.0	1.4818853982527465	0.0
58-59	0.0	1.9771125497021382E-5	0.0	1.7095894506019418	0.0
60-61	0.0	1.9771125497021382E-5	0.0	1.9464574196190063	0.0
62-63	0.0	1.9771125497021382E-5	0.0	2.1928649566883838	0.0
64-65	0.0	1.9771125497021382E-5	0.0	2.452825600285969	0.0
66-67	0.0	1.9771125497021382E-5	0.0	2.759307702178046	0.0
68-69	0.0	1.9771125497021382E-5	0.0	3.1058757610153345	0.0
70-71	0.0	1.9771125497021382E-5	0.0	3.47144387145526	0.0
72-73	0.0	1.9771125497021382E-5	0.0	3.8176659355963496	0.0
74-75	0.0	1.9771125497021382E-5	0.0	4.192210137011923	0.0
76-77	0.0	1.9771125497021382E-5	0.0	4.576788184617234	0.0
78-79	0.0	2.9656688245532074E-5	0.0	5.093140783660193	0.0
80-81	0.0	3.9542250994042765E-5	0.0	5.6230761459196055	0.0
82-83	0.0	3.9542250994042765E-5	0.0	6.108289222304756	0.0
84-85	0.0	3.9542250994042765E-5	0.0	6.60482126803695	0.0
86-87	0.0	3.9542250994042765E-5	0.0	7.102341870043997	0.0
88	0.0	3.9542250994042765E-5	0.0	7.522458515730204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	2700	0.0	24.733442	1
GTACGCA	2715	0.0	23.556093	3
CGCAGTG	2865	0.0	22.474035	6
TACGCAG	2855	0.0	22.40098	4
GAGTACT	12630	0.0	22.252054	12-13
GTACTAG	1710	0.0	22.00158	1
AGTACGC	3075	0.0	21.104792	2
GTACTTT	14055	0.0	20.096388	14-15
CATGGGG	8065	0.0	18.542017	4
GTGATCG	1245	0.0	18.119413	8
AGTACTT	13655	0.0	18.069239	12-13
GTACCCG	540	0.0	17.417917	1
GGGCTAT	2515	0.0	17.379211	6
GTATAAG	2505	0.0	17.271898	1
TGATCGC	1310	0.0	17.220358	9
ACTTTTT	16575	0.0	17.026917	16-17
CTATTGA	2525	0.0	16.937614	9
GCTATTG	2565	0.0	16.856707	8
TAACGCC	755	0.0	16.817097	4
CTAACGC	765	0.0	16.597265	3
>>END_MODULE