FastQCFastQC Report
Mon 16 Jan 2017
ERR1146606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259582
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3074411.843656339807845No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT205207.905016526569638No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC123094.741854211771232No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT101053.8927968811396783No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC75122.8938832430599963No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT60772.3410714148130456No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49811.9188541578383709No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45581.7558998697906634No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC44711.722384448844681No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC43871.6900247320692496No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42131.622993890177285No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41101.58331471365503No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC40451.5582744566264226No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC38051.465818122982333No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35261.3583376351210792No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30801.18652294843248No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27431.0566988466072378No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG22840.879876108512917No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG21340.822090899985361No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC19810.7631499872872541No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT19570.7539043539228453No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19010.732331209405891No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT18810.7246265149355502No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA12810.49348568082532684No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT11590.4464870445562481No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG10710.4125863888867487No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC9990.3848494887935219No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC9570.36866963040580625No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8970.3455555469947839No Hit
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8700.3351542094598239No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7860.3027944926843926No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG7610.2931636245964666No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT7450.28699986902019403No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7170.2762132967617169No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA5860.22574754798098481No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT5620.2165019146165759No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5160.1987811173347921No Hit
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4770.18375696311762757No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT4650.1791341464354231No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT4480.17258515613563344No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA4060.1564052977479178No Hit
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4020.15486435885384967No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA3550.13675832684854883No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3240.12481605041952061No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGT3090.11903752956676503No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC2850.10979189620235609No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT2800.10786572258477091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG250.005196568656.49548394
TAGCCAG250.005224434756.419215
GAGTACG8850.048.353471
GTACGCA9150.047.2820433
GCTTATG1500.047.0250781
AGTACGC9200.047.0250782
CGCGTAA253.0045006E-547.00694392-93
TACGCAG9150.046.768114
CGCAGTG9450.046.234066
ACGCAGT9800.044.6172335
GCTTGCC450.001009847241.800073
TCTGTAT450.001009847241.800073
ATATTCC450.001009847241.800074
ACCTGGG1600.038.2078743
TTATGTA1850.038.128443
TCTTACA500.001695149737.6200642
TACCTGG1900.037.125062
CTTATGT1950.036.1731382
GCCTACT2600.036.1731343
CTGCCTA2650.035.4906231
Produced by FastQC (version 0.11.5)