##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146606_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259582
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302155003043353	31.0	31.0	34.0	30.0	34.0
2	31.34112534767434	31.0	31.0	34.0	28.0	34.0
3	31.57564083796257	33.0	31.0	34.0	28.0	34.0
4	34.76445978534721	37.0	35.0	37.0	32.0	37.0
5	35.05813962447319	37.0	35.0	37.0	33.0	37.0
6	35.29252413495543	37.0	35.0	37.0	33.0	37.0
7	35.30391937807706	37.0	35.0	37.0	33.0	37.0
8	35.33256543211779	37.0	35.0	37.0	33.0	37.0
9	36.92762210014562	39.0	37.0	39.0	33.0	39.0
10-11	36.87307478946923	39.0	37.0	39.0	33.0	39.0
12-13	36.91129392638935	39.0	37.0	39.0	33.0	39.0
14-15	37.83030410429075	40.0	38.0	41.0	32.5	41.0
16-17	38.18311169495574	40.0	38.0	41.0	33.0	41.0
18-19	38.35771355486898	40.0	38.5	41.0	33.5	41.0
20-21	38.302166560085055	40.0	38.5	41.0	33.5	41.0
22-23	38.20950219969027	40.0	38.0	41.0	33.0	41.0
24-25	38.04827954172477	40.0	38.0	41.0	32.5	41.0
26-27	37.67287600835189	40.0	37.5	41.0	31.5	41.0
28-29	37.74743241056776	40.0	37.5	41.0	32.0	41.0
30-31	37.85567373700796	40.0	38.0	41.0	32.0	41.0
32-33	37.568793290752055	40.0	38.0	41.0	31.0	41.0
34-35	36.97945543219484	39.0	36.5	41.0	30.0	41.0
36-37	36.7208203958672	39.0	36.0	40.5	30.0	41.0
38-39	36.545567489271214	39.0	36.0	40.0	30.0	41.0
40-41	36.86860414050281	39.5	36.5	41.0	30.0	41.0
42-43	36.5822379826028	39.0	36.0	41.0	29.5	41.0
44-45	36.15843741091447	39.0	35.0	40.0	28.0	41.0
46-47	35.66815880916242	38.0	34.5	40.0	27.0	41.0
48-49	35.32638434097896	38.0	33.5	40.0	26.0	41.0
50-51	34.751546717414925	38.0	33.0	40.0	23.5	41.0
52-53	34.927161744651016	38.0	33.0	40.0	24.5	41.0
54-55	34.575767965421335	38.0	32.5	40.0	23.5	41.0
56-57	33.26252398086154	36.5	29.5	39.5	20.0	41.0
58-59	32.784291668913866	36.0	30.0	39.0	20.0	40.5
60-61	31.920096539821714	35.0	28.0	39.0	16.0	40.0
62-63	32.435696619950534	35.5	29.5	39.0	19.5	40.0
64-65	32.318737431717146	35.5	29.5	38.5	19.5	39.5
66-67	31.59800563983635	35.0	28.5	38.0	18.5	39.0
68-69	30.586099960706058	34.0	27.0	37.0	15.5	39.0
70-71	29.68577174072162	33.0	26.0	36.0	13.0	38.0
72-73	29.504066152506724	32.5	26.0	35.0	14.0	37.0
74-75	29.54262622215716	33.0	26.0	35.0	13.5	37.0
76-77	29.4073452704733	33.0	26.0	35.0	13.0	36.5
78-79	28.692420892049526	32.0	26.0	35.0	12.0	36.0
80-81	27.527538504210618	31.0	24.0	34.0	11.0	35.5
82-83	27.047944387515315	30.5	22.0	34.0	10.5	35.0
84-85	27.33061807059041	30.5	24.0	34.0	11.0	35.0
86-87	28.023291291383842	31.5	25.0	34.5	11.0	35.0
88-89	27.748441725543373	31.0	24.5	34.0	10.0	35.0
90-91	27.121870930958234	31.0	23.0	34.0	10.0	35.0
92-93	26.22201269733649	30.0	20.0	34.0	7.5	35.0
94-95	26.435122620212496	30.5	21.5	34.0	8.5	35.0
96-97	26.498484101362962	31.0	21.0	34.0	7.5	35.0
98-99	26.926662865684058	31.0	23.5	34.0	8.0	35.0
100	25.27788521546178	29.0	19.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1183827954783645
1101	2	0.38794433668621764
1101	3	0.4254919988656134
1101	4	0.14086103566750552
1101	5	0.12611411745098877
1101	6	0.016143800767089544
1101	7	0.21316231617508663
1101	8	0.1653107454170808
1101	9	0.45997170971579493
1101	10-11	0.36665269099672315
1101	12-13	0.4196824554807179
1101	14-15	0.6958137546870091
1101	16-17	0.5625367535738945
1101	18-19	0.48704894099280693
1101	20-21	0.5897003482134906
1101	22-23	0.6312772541501843
1101	24-25	0.6670247765067288
1101	26-27	0.7808285646364652
1101	28-29	0.5890449253749992
1101	30-31	0.689281163675318
1101	32-33	0.7417512656912493
1101	34-35	0.7309945447347346
1101	36-37	0.7582004944963145
1101	38-39	0.7761854999127493
1101	40-41	0.7071413880318005
1101	42-43	0.5290151804579537
1101	44-45	0.6136161456082831
1101	46-47	1.0581118433408179
1101	48-49	1.198637192408647
1101	50-51	0.9072744916234825
1101	52-53	1.1321581246356587
1101	54-55	1.3697677706066003
1101	56-57	1.3733274951248617
1101	58-59	1.921585556357698
1101	60-61	2.1558706021876652
1101	62-63	2.2912992152536233
1101	64-65	2.0094785247177285
1101	66-67	1.9380193181116674
1101	68-69	1.9245437795272586
1101	70-71	1.7144483032199425
1101	72-73	1.298385821723027
1101	74-75	1.4911789271428724
1101	76-77	1.6039456807391304
1101	78-79	1.438233865516846
1101	80-81	1.4405604801678713
1101	82-83	1.5789367881557865
1101	84-85	1.6383930171692285
1101	86-87	1.51829632274546
1101	88-89	1.5969780058372685
1101	90-91	1.5272020409837133
1101	92-93	1.3627488056826493
1101	94-95	1.8692041909625345
1101	96-97	1.5002518371101026
1101	98-99	1.2879097761620173
1101	100	1.190201546342994
1102	1	0.46561740215255654
1102	2	0.6154096755642797
1102	3	0.5587311665252308
1102	4	0.5079493436050413
1102	5	0.5689479255581844
1102	6	0.6478402821798852
1102	7	0.562006682411166
1102	8	0.6325671311912231
1102	9	0.8376576014357937
1102	10-11	0.7801675694361947
1102	12-13	0.7909450909096307
1102	14-15	0.7463120699631531
1102	16-17	0.6350706255980114
1102	18-19	0.6380112457897411
1102	20-21	0.7018311490688731
1102	22-23	0.9756040353412416
1102	24-25	0.995624838056365
1102	26-27	0.9038449699802342
1102	28-29	0.8927686780086077
1102	30-31	0.45512427524585775
1102	32-33	0.6969818006206623
1102	34-35	0.7004327859934989
1102	36-37	0.8741584198003167
1102	38-39	0.5394443161081881
1102	40-41	1.038045615228846
1102	42-43	0.7978805366961694
1102	44-45	1.0521769889960382
1102	46-47	1.0158398728846407
1102	48-49	0.9739218594488293
1102	50-51	0.8167018433322752
1102	52-53	1.0554380886527994
1102	54-55	0.8623029051872351
1102	56-57	0.8978605464872373
1102	58-59	0.8223249410192182
1102	60-61	0.5145392363382406
1102	62-63	1.0329525330154254
1102	64-65	1.0284484364176834
1102	66-67	1.0346206737817347
1102	68-69	1.1893216090347813
1102	70-71	1.1042665739909694
1102	72-73	0.5083608073219921
1102	74-75	0.9517319268982511
1102	76-77	1.0892368972912472
1102	78-79	1.1675575297715035
1102	80-81	1.2166894818802163
1102	82-83	0.9153449726784189
1102	84-85	0.5657526169388127
1102	86-87	1.0672361698182975
1102	88-89	0.8675320314198167
1102	90-91	1.3569778582917849
1102	92-93	1.313674811979645
1102	94-95	0.4337366110466512
1102	96-97	0.824212003451926
1102	98-99	1.1114319932112657
1102	100	1.4643972583854143
1103	1	0.24742448587064558
1103	2	0.25493982753710753
1103	3	0.5083187582801934
1103	4	0.5088414601123219
1103	5	0.36462194305618567
1103	6	0.6846674776473094
1103	7	0.21158488346605253
1103	8	0.5838998280437764
1103	9	0.6694701630622646
1103	10-11	0.6160024809288487
1103	12-13	0.49971600478126277
1103	14-15	0.7961923385371747
1103	16-17	0.7771714817168274
1103	18-19	0.7476985354771841
1103	20-21	0.7214929273544399
1103	22-23	0.6574549423263605
1103	24-25	0.8514228127953771
1103	26-27	0.8417059250144305
1103	28-29	0.811124366425183
1103	30-31	0.9319018565145427
1103	32-33	0.9327783667546683
1103	34-35	0.8218623635685134
1103	36-37	1.2127127215235092
1103	38-39	1.1809778174307155
1103	40-41	1.0948358739426283
1103	42-43	1.3060739225130789
1103	44-45	1.3562791598128712
1103	46-47	1.3301035637427887
1103	48-49	1.2163506075119912
1103	50-51	1.037070271798619
1103	52-53	1.074951180853823
1103	54-55	1.2297609991881373
1103	56-57	1.5644046123473458
1103	58-59	1.5721919061014589
1103	60-61	1.465801502940213
1103	62-63	1.422095318610097
1103	64-65	1.1866934710850146
1103	66-67	1.4903669996703321
1103	68-69	1.9093965781237294
1103	70-71	1.5181695054545088
1103	72-73	1.5489090676948045
1103	74-75	1.3681468706323088
1103	76-77	1.4978575599616484
1103	78-79	1.4514227419230643
1103	80-81	1.4150445442501152
1103	82-83	1.6593897037131171
1103	84-85	1.4756058089942776
1103	86-87	1.3507883586339737
1103	88-89	0.9643736259606897
1103	90-91	1.2828500195614083
1103	92-93	1.3659575346566228
1103	94-95	1.4137070303414099
1103	96-97	1.0294535792113955
1103	98-99	1.1495073692305482
1103	100	1.1228105430957918
1104	1	-0.040923642504797186
1104	2	-0.10602298828013446
1104	3	0.08003332777551009
1104	4	0.11218431193428557
1104	5	0.1420134633243606
1104	6	-0.059714544824601035
1104	7	-0.05489658651966778
1104	8	0.29303757712813905
1104	9	0.07278508018933394
1104	10-11	0.02742621921179733
1104	12-13	-0.08023929970691768
1104	14-15	0.0333501385247601
1104	16-17	0.38501492327124254
1104	18-19	0.1616131173879154
1104	20-21	-0.0358864878792744
1104	22-23	0.1917370206676665
1104	24-25	0.11996834634805253
1104	26-27	0.039149900431901585
1104	28-29	0.3618180780149771
1104	30-31	-0.07990055207692137
1104	32-33	0.04188662921664843
1104	34-35	0.21022445929139622
1104	36-37	-0.19133160152572515
1104	38-39	0.10193039052649766
1104	40-41	-0.05366030831573454
1104	42-43	0.31048915623335915
1104	44-45	0.17023409183844507
1104	46-47	0.11721015012307845
1104	48-49	0.1033780455900839
1104	50-51	0.5154596814453996
1104	52-53	0.636958443874498
1104	54-55	0.5135387010939141
1104	56-57	0.5132996399716561
1104	58-59	0.4861375265620751
1104	60-61	0.6890307536696234
1104	62-63	0.6920065483574405
1104	64-65	0.4711307421757098
1104	66-67	0.6839466756278689
1104	68-69	0.564946270842082
1104	70-71	0.4954958447679374
1104	72-73	0.4888649929539781
1104	74-75	0.6064530969654136
1104	76-77	0.23710270242859366
1104	78-79	0.14954180580039989
1104	80-81	0.6280233138820002
1104	82-83	0.4521841043587216
1104	84-85	0.5665642794145391
1104	86-87	0.47654880310600944
1104	88-89	-0.18250855170396818
1104	90-91	-0.010844499030682186
1104	92-93	-0.25849153108302403
1104	94-95	0.13294006777390166
1104	96-97	0.19387253755765954
1104	98-99	-0.3901754167896918
1104	100	-0.3058783885573213
1105	1	-0.11807319486915446
1105	2	-0.24504540010492804
1105	3	-0.08810042214938463
1105	4	-0.41784332731344165
1105	5	-0.09928918643830542
1105	6	-0.16827369882327758
1105	7	0.058469393171492356
1105	8	0.2017980169526865
1105	9	-0.03951438505130511
1105	10-11	0.21511023050783962
1105	12-13	0.15326727963575593
1105	14-15	0.2604801631597411
1105	16-17	0.13871017052233725
1105	18-19	0.11078093568990965
1105	20-21	0.11522058416301917
1105	22-23	0.003955370724746388
1105	24-25	-0.02746601043858732
1105	26-27	0.06475375790951432
1105	28-29	0.008734198906644508
1105	30-31	0.17495884827157226
1105	32-33	0.37212306495539593
1105	34-35	0.2469591225131751
1105	36-37	0.3268291867936952
1105	38-39	0.7205012611628021
1105	40-41	0.3534703171724658
1105	42-43	0.36183957382634446
1105	44-45	0.2786430325342337
1105	46-47	0.10972952763915345
1105	48-49	0.26430906764139905
1105	50-51	0.35628615503790684
1105	52-53	0.7496860170283739
1105	54-55	0.575884732458448
1105	56-57	0.2673103351588182
1105	58-59	-0.01933023766312658
1105	60-61	0.7306698812307566
1105	62-63	1.031248271604447
1105	64-65	0.606427864088829
1105	66-67	0.9934987395586496
1105	68-69	0.32984743048877974
1105	70-71	-0.22877261644698876
1105	72-73	0.22872559500889267
1105	74-75	0.11233394777356054
1105	76-77	0.392663598901585
1105	78-79	0.2868217433116662
1105	80-81	0.030978009549848196
1105	82-83	0.11267950783165404
1105	84-85	0.44281856049367363
1105	86-87	0.033832646163151736
1105	88-89	-0.03407527645311248
1105	90-91	0.23501712447469458
1105	92-93	0.24191404483477896
1105	94-95	-0.05713233503809079
1105	96-97	-0.13649909015368777
1105	98-99	0.1247858281781653
1105	100	-0.026316410548549385
1106	1	0.10380549570023945
1106	2	-0.09220109071412708
1106	3	-0.06394999951698921
1106	4	0.007024973266432255
1106	5	-0.017018515932335276
1106	6	-0.18630026428817104
1106	7	-0.16659396851425612
1106	8	-0.2827563281329617
1106	9	0.04300840525137062
1106	10-11	-0.07946521302395837
1106	12-13	-0.16239002321319873
1106	14-15	-0.14758567991558635
1106	16-17	-0.06839629037919082
1106	18-19	-0.2680157894066326
1106	20-21	-0.1421506025250565
1106	22-23	-0.3886084222185602
1106	24-25	-0.285752790072614
1106	26-27	-0.16941577274957353
1106	28-29	-0.04926662380006519
1106	30-31	-0.07501793016820812
1106	32-33	-0.013683253962881281
1106	34-35	0.03276676837774062
1106	36-37	0.04353610416474396
1106	38-39	-0.21923879005732516
1106	40-41	-0.12850975209413207
1106	42-43	0.005435067516714298
1106	44-45	-0.16956293607021422
1106	46-47	0.11914089426114316
1106	48-49	0.22522054705383
1106	50-51	0.1652456964452682
1106	52-53	0.07120510515087375
1106	54-55	0.10589030563290436
1106	56-57	-0.23794105481741923
1106	58-59	0.49342835099679405
1106	60-61	0.22665586867784882
1106	62-63	0.050455819274993985
1106	64-65	0.004664352458142673
1106	66-67	-0.053879878028695316
1106	68-69	0.20430835855502139
1106	70-71	0.3299746135526149
1106	72-73	0.25896006641983504
1106	74-75	0.26524460447928533
1106	76-77	0.39900640220833594
1106	78-79	-0.09106934093615493
1106	80-81	0.34592207876336545
1106	82-83	0.284854107425609
1106	84-85	0.18068031922829775
1106	86-87	-0.0202205113055669
1106	88-89	0.11011070834008407
1106	90-91	0.19052793245229438
1106	92-93	0.19534486448950616
1106	94-95	0.19537559346604283
1106	96-97	0.22119918879696598
1106	98-99	0.4843750056041536
1106	100	0.36025621985709577
1107	1	0.49905810619765134
1107	2	0.436026290223257
1107	3	0.4097686839609871
1107	4	0.3932400354271266
1107	5	0.3248401330329074
1107	6	0.39025123399248685
1107	7	0.4686732532111293
1107	8	0.4237910135052587
1107	9	0.5017190903416164
1107	10-11	0.5783801346854816
1107	12-13	0.5504143248867095
1107	14-15	0.5539327541638457
1107	16-17	0.4842135772380445
1107	18-19	0.5495957786552452
1107	20-21	0.6149219875670227
1107	22-23	0.6910668558356363
1107	24-25	0.7997008507682821
1107	26-27	0.8314344495678228
1107	28-29	0.7364616073596437
1107	30-31	0.7895385056998379
1107	32-33	0.8410011561087885
1107	34-35	0.7612781865485729
1107	36-37	0.7718806268436325
1107	38-39	0.8580117799065263
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2216	84-85	-3.9865476141286678
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2216	88-89	-4.185440514630496
2216	90-91	-4.146497944896161
2216	92-93	-4.445917099353103
2216	94-95	-4.39173890493878
2216	96-97	-4.207638102943555
2216	98-99	-4.338914608008039
2216	100	-4.120601631968498
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	71.0
1	21.0
2	19.0
3	18.0
4	11.0
5	17.0
6	9.0
7	61.0
8	136.0
9	256.0
10	369.0
11	502.0
12	651.0
13	794.0
14	795.0
15	808.0
16	1005.0
17	1060.0
18	1232.0
19	1525.0
20	1613.0
21	2078.0
22	2509.0
23	2901.0
24	3621.0
25	4142.0
26	4880.0
27	5546.0
28	6613.0
29	7831.0
30	9770.0
31	12142.0
32	15331.0
33	20202.0
34	26370.0
35	32892.0
36	36320.0
37	33745.0
38	18376.0
39	3322.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.277279948177735	14.54647809121335	11.929530977682496	17.246710982926416
2	11.597990802098632	33.016456764852144	25.16123312015481	30.22431931289441
3	40.96268478319543	16.978076635384294	30.379629701031007	11.679608880389269
4	9.710617131620044	12.052285565559389	49.23480306155893	29.002294241261637
5	8.647035461867985	48.884321898291496	31.8304678252085	10.63817481463202
6	23.38289848142464	36.4095603149752	14.547165300283046	25.66037590331711
7	26.33167921712878	31.705821795005264	23.1927141772025	18.76978481066346
8	20.518495952985997	16.594107054028314	18.4462088405758	44.44118815240989
9	13.805573118262988	30.29361419957747	31.952519006276315	23.948293675883235
10-11	30.069208821714994	16.928979025293028	27.17169185687847	25.830120296113513
12-13	41.80342404231286	18.330885385946853	20.929757402631445	18.935933169108832
14-15	24.221493975903616	24.984289156626506	36.790361445783134	14.003855421686747
16-17	18.26301017064162	25.632869909859895	33.66682859500181	22.437291324496673
18-19	14.90033542815283	43.89674981686394	21.32937502409685	19.873539730886378
20-21	23.79095449489858	24.87138018840465	17.695464525219894	33.64220079147688
22-23	17.791725621580774	28.12081934158124	22.37690792238385	31.71054711445414
24-25	31.36027944688948	26.68393932208683	18.994523257360637	22.961257973663056
26-27	26.857852189098647	15.837377939005162	37.18788430178991	20.116885570106284
28-29	16.145337337923962	41.361342334666055	23.425312971095234	19.068007356314748
30-31	23.01088825656804	22.393411525204154	22.466089866672835	32.12961035155497
32-33	25.95812031060834	25.6201756618163	26.849152921399433	21.572551106175926
34-35	32.451778949499236	19.96703328481507	26.674892279812223	20.906295485873475
36-37	26.516873427604754	16.788409820421617	37.00895482104817	19.68576193092546
38-39	20.744604968402868	29.855335654440367	31.550476366734912	17.84958301042185
40-41	20.29814435692502	33.113185359967	23.688847249692525	22.899823033415455
42-43	17.870031925230183	31.31120930304292	17.002691281481823	33.81606749024507
44-45	24.670249804328297	21.526971850263916	19.632908317686024	34.169870027721764
46-47	31.52552958934055	29.745292057268323	23.29961773567665	15.429560617714472
48-49	27.2604585792257	26.314419623319562	25.592407010752105	20.83271478670263
50-51	11.907534286187978	30.530226209800237	37.57002787620344	19.992211627808345
52-53	22.97618014787395	33.15576891562316	21.063265758601077	22.80478517790181
54-55	16.66078695590988	23.6183161665057	26.30100649081512	33.4198903867693
56-57	29.171775592828226	17.284557547715444	26.97108155002892	26.572585309427417
58-59	40.16052410367937	19.168421133809932	21.16620009408571	19.50485466842499
60-61	23.934703729838322	25.302015493115242	36.239738413902934	14.523542363143507
62-63	18.47035392514301	25.224565185118063	33.27690685196146	23.028174037777465
64-65	15.124038166776241	42.479143811780496	21.575719483063445	20.82109853837982
66-67	23.523480098408953	24.615541827660937	18.10462506651859	33.75635300741152
68-69	17.92900275320629	27.553463872843516	22.82114956002684	31.696383813923358
70-71	30.669414842850724	26.335060262567794	19.445210326098483	23.550314568482992
72-73	26.3810162799127	16.3275339518312	36.351209618335915	20.940240149920182
74-75	16.387000992952927	40.11722628722369	23.74487858017372	19.75089413964967
76-77	22.77866251248376	22.2569475238782	22.906102870010837	32.058287093627214
78-79	25.48555342389156	25.153142383371733	26.91584640450413	22.445457788232574
80-81	31.67616238154457	19.9624027532753	26.512807164685583	21.84862770049455
82-83	25.9222767456882	17.224444937190896	35.691960627416194	21.161317689704706
84-85	20.54846023923556	29.068624048494947	31.11141960312502	19.271496109144476
86-87	20.183967347820136	32.08400571451707	24.22731593143351	23.504711006229286
88-89	18.065947794703124	30.508422292614178	17.845389187615556	33.58024072506714
90-91	24.237761968110743	21.3548113444001	20.287658819673396	34.11976786781576
92-93	30.392412018160265	28.68299547921305	23.547805635078994	17.37678686754769
94-95	26.375592321127694	25.617189939890732	25.654975111909657	22.35224262707192
96-97	12.722761588229398	29.510560692170667	36.37308276590658	21.393594953693352
98-99	22.848455283925816	31.9470768684962	21.751353060606704	23.453114786971284
100	21.180700372275517	15.126589122140352	20.621900922192875	43.07080958339126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	76.0
2	45.5
3	33.0
4	28.0
5	29.0
6	26.0
7	26.0
8	24.5
9	19.0
10	14.5
11	13.0
12	18.0
13	21.5
14	22.5
15	24.5
16	21.5
17	23.5
18	38.5
19	56.5
20	65.5
21	75.0
22	103.5
23	155.0
24	199.0
25	241.0
26	327.0
27	420.0
28	466.5
29	513.5
30	615.5
31	734.5
32	872.0
33	993.0
34	1162.5
35	1315.0
36	1398.0
37	1540.5
38	1611.0
39	1606.5
40	1612.5
41	1668.5
42	1730.5
43	1837.5
44	2148.5
45	2790.0
46	5275.0
47	22542.5
48	62879.0
49	69279.5
50	34965.0
51	13908.5
52	6570.5
53	3839.5
54	2613.5
55	2030.0
56	1677.5
57	1405.5
58	1137.5
59	941.5
60	806.5
61	652.0
62	502.5
63	399.0
64	313.0
65	218.5
66	161.0
67	129.5
68	101.0
69	75.0
70	59.0
71	60.0
72	52.0
73	40.0
74	35.0
75	27.0
76	20.0
77	14.5
78	8.5
79	6.5
80	4.5
81	2.0
82	2.5
83	2.5
84	1.0
85	2.0
86	2.5
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09014492530298711
2	0.06741607661548181
3	0.08629257806781672
4	0.09130062947353823
5	0.08975969057947007
6	0.10016102811443012
7	0.08821875168540191
8	0.09823485449684494
9	0.07473553636230555
10-11	0.08552210862078265
12-13	0.06972748495658404
14-15	0.07974358776802706
16-17	0.0801288224915441
18-19	0.08166976138561226
20-21	0.07454291900054703
22-23	0.0782026488739589
24-25	0.08186237874737078
26-27	0.07049795440361813
28-29	0.0820549961091293
30-31	0.08475163917374856
32-33	0.08475163917374856
34-35	0.08841136904716043
36-37	0.08648519542957524
38-39	0.08590734334429968
40-41	0.08128452666209522
42-43	0.08706304751485079
44-45	0.08436640445023152
46-47	0.07955097040626855
48-49	0.09149324683529675
50-51	0.0851368738972656
52-53	0.09226371628233082
54-55	0.08301808291792188
56-57	0.08937445585595304
58-59	0.0939972725381575
60-61	0.09284156836760638
62-63	0.09534559407046714
64-65	0.0949603593469501
66-67	0.09862008922036196
68-69	0.09553821143222566
70-71	0.09958317602915456
72-73	0.0939972725381575
74-75	0.0980422371350864
76-77	0.09438250726167455
78-79	0.1018945843702568
80-81	0.0980422371350864
82-83	0.1018945843702568
84-85	0.09862008922036196
86-87	0.09341942045288194
88-89	0.09264895100584786
90-91	0.09515297670870862
92-93	0.08648519542957524
94-95	0.0851368738972656
96-97	0.08629257806781672
98-99	0.10112411492322272
100	0.24423881470980266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	259582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.88378238860938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.76734733378497	20.962162245456156
2	6.643762702022646	3.1735636523333666
3	1.8274783057517983	1.3094128252344153
4	0.8693828833188166	0.830566063902736
5	0.5548566082776863	0.6626037244493069
6	0.3935610826155682	0.563983635228945
7	0.2919449014484338	0.48809239469608834
8	0.17581212297170876	0.33592467890685795
9	0.14194006258266395	0.30510590102549484
>10	1.0468079615471466	4.7433951506653
>50	0.1193586889899674	1.9932044594771596
>100	0.10806800219361914	4.8316139023507025
>500	0.017742507822832994	3.2344307386490585
>1k	0.032259105132423624	22.947662010462974
>5k	0.0032259105132423625	5.234954657873042
>10k+	0.006451821026484725	28.383323959288393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	30744	11.843656339807845	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	20520	7.905016526569638	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	12309	4.741854211771232	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10105	3.8927968811396783	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	7512	2.8938832430599963	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	6077	2.3410714148130456	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	4981	1.9188541578383709	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4558	1.7558998697906634	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	4471	1.722384448844681	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	4387	1.6900247320692496	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4213	1.622993890177285	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	4110	1.58331471365503	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4045	1.5582744566264226	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	3805	1.465818122982333	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	3526	1.3583376351210792	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	3080	1.18652294843248	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	2743	1.0566988466072378	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	2284	0.879876108512917	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2134	0.822090899985361	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	1981	0.7631499872872541	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	1957	0.7539043539228453	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	1901	0.732331209405891	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	1881	0.7246265149355502	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1281	0.49348568082532684	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	1159	0.4464870445562481	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1071	0.4125863888867487	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	999	0.3848494887935219	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	957	0.36866963040580625	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	897	0.3455555469947839	No Hit
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	870	0.3351542094598239	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	786	0.3027944926843926	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	761	0.2931636245964666	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	745	0.28699986902019403	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	717	0.2762132967617169	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	586	0.22574754798098481	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	562	0.2165019146165759	No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	516	0.1987811173347921	No Hit
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	477	0.18375696311762757	No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT	465	0.1791341464354231	No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	448	0.17258515613563344	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	406	0.1564052977479178	No Hit
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	402	0.15486435885384967	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	355	0.13675832684854883	No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	324	0.12481605041952061	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGT	309	0.11903752956676503	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC	285	0.10979189620235609	No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT	280	0.10786572258477091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.8523472351703896E-4	0.0
10-11	0.0	0.0	0.0	3.8523472351703896E-4	0.0
12-13	0.0	0.0	0.0	3.8523472351703896E-4	0.0
14-15	0.0	0.0	0.0	3.8523472351703896E-4	0.0
16-17	0.0	0.0	0.0	5.778520852755584E-4	0.0
18-19	0.0	0.0	0.0	9.630868087925974E-4	0.0
20-21	0.0	0.0	0.0	0.0011557041705511169	0.0
22-23	0.0	0.0	0.0	0.0013483215323096365	0.0
24-25	0.0	0.0	0.0	0.0015409388940681558	0.0
26-27	0.0	0.0	0.0	0.0019261736175851948	0.0
28-29	0.0	0.0	0.0	0.005778520852755584	0.0
30-31	0.0	0.0	0.0	0.013483215323096362	0.0
32-33	0.0	0.0	0.0	0.03158924732839719	0.0
34-35	0.0	0.0	0.0	0.05682212171876325	0.0
36-37	0.0	0.0	0.0	0.08687043015309229	0.0
38-39	0.0	0.0	0.0	0.13598785740151476	0.0
40-41	0.0	0.0	0.0	0.18317911103235202	0.0
42-43	0.0	0.0	0.0	0.23210392091901594	0.0
44-45	0.0	0.0	0.0	0.3024092579608756	0.0
46-47	0.0	0.0	0.0	0.3981400867548598	0.0
48-49	0.0	0.0	0.0	0.4751870314582675	0.0
50-51	0.0	0.0	0.0	0.5580124970144309	0.0
52-53	0.0	0.0	0.0	0.6439198403587306	0.0
54-55	0.0	0.0	0.0	0.766809717160666	0.0
56-57	0.0	0.0	0.0	0.9771478762009692	0.0
58-59	0.0	0.0	0.0	1.143184042036813	0.0
60-61	0.0	0.0	0.0	1.2945812883790093	0.0
62-63	0.0	0.0	0.0	1.4577281937884754	0.0
64-65	0.0	0.0	0.0	1.5954496074458167	0.0
66-67	0.0	0.0	0.0	1.7872964997573022	0.0
68-69	0.0	0.0	0.0	2.0452111471519596	0.0
70-71	0.0	0.0	0.0	2.2734627208358056	0.0
72-73	0.0	0.0	0.0	2.4558713624211235	0.0
74-75	0.0	0.0	0.0	2.6744920680170425	0.0
76-77	0.0	0.0	0.0	2.8713470117342497	0.0
78-79	0.0	0.0	0.0	3.322456872972702	0.0
80-81	0.0	0.0	0.0	3.726568097942076	0.0
82-83	0.0	0.0	0.0	3.9839048932514585	0.0
84-85	0.0	0.0	0.0	4.287662472744643	0.0
86-87	0.0	0.0	0.0	4.564453621591635	0.0
88	0.0	0.0	0.0	4.821983034262776	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	25	0.0051965686	56.495483	94
TAGCCAG	25	0.0052244347	56.41921	5
GAGTACG	885	0.0	48.35347	1
GTACGCA	915	0.0	47.282043	3
GCTTATG	150	0.0	47.025078	1
AGTACGC	920	0.0	47.025078	2
CGCGTAA	25	3.0045006E-5	47.006943	92-93
TACGCAG	915	0.0	46.76811	4
CGCAGTG	945	0.0	46.23406	6
ACGCAGT	980	0.0	44.617233	5
GCTTGCC	45	0.0010098472	41.80007	3
TCTGTAT	45	0.0010098472	41.80007	3
ATATTCC	45	0.0010098472	41.80007	4
ACCTGGG	160	0.0	38.207874	3
TTATGTA	185	0.0	38.12844	3
TCTTACA	50	0.0016951497	37.620064	2
TACCTGG	190	0.0	37.12506	2
CTTATGT	195	0.0	36.173138	2
GCCTACT	260	0.0	36.173134	3
CTGCCTA	265	0.0	35.490623	1
>>END_MODULE