FastQCFastQC Report
Mon 16 Jan 2017
ERR1146606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259582
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3093211.91608046782905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT204697.88536955567027No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC124084.779992449399419No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT102383.944033099367444No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC78903.039501968549437No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT61872.38344723439992No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA52852.0359655137875508No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC50501.9454353537610467No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA49741.9161575147737517No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC46141.7774730143076174No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC42951.6545831375056823No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42841.6503455555469948No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40751.5698314983319337No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC39011.5028006564399687No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG38731.4920140841814917No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30731.1838263053678606No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC29031.118336402369964No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG24270.9349646739758535No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG23490.9049163655415245No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT20400.7858788359747595No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC19750.7608385789461519No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19510.7515929455817429No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18490.712299003783005No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA15400.59326147421624No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG12690.4888628641431224No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT10960.4222172569746747No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10110.38947230547572637No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC9170.3532602414651247No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG8890.3424736692066476No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG7960.306646839919563No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC7410.28545893012612583No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7180.27659853148523394No Hit
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7120.27428712314413173No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT6730.2592629689269672No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA6460.24886163139200718No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT6160.23730458968649598No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5360.2064858118051329No Hit
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4590.17682273809432086No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT4450.17142945196508233No Hit
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4170.16064287970660523No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT4160.1602576449830882No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA3930.1513972463421963No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA3820.14715966438350886No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3410.13136504071931027No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGT3330.12828316293117395No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC3100.11942276429028208No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT3030.1167261212256628No Hit
AGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA2910.11210330454345832No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG2870.11056236564939018No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG2680.10324290590256645No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC2630.10131673228498124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC255.5263292E-575.268343
TAGTAGT200.002153314970.5640644
TAAAGCT250.005212708456.4512564
CTCTTAC250.005212708456.4512561
ACCCAGT250.005212708456.4512563
GTACCCA602.5274858E-854.883161
GAGTACG9450.054.758711
TACGCAG9600.053.9031074
GTACGCA9600.053.9031073
CGCAGTG9600.053.861566
AGTACGC9850.052.535012
ACGCAGT9950.051.966935
ACCCATG405.6432193E-447.042713
TACCCAT450.001007986741.8157422
TACCTGG1253.6379788E-1241.3975832
GTACCTG1401.2732926E-1136.9621281
ACCTGGG1302.2555469E-1036.1867033
AGTATGC550.00270109234.2128894
CCAGTGG600.0041523131.3376355
AGTAGGC3050.030.8476772
Produced by FastQC (version 0.11.5)