##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259582
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.320811920703285	33.0	31.0	34.0	30.0	34.0
2	32.23005832453714	33.0	31.0	34.0	30.0	34.0
3	32.47691288301962	34.0	31.0	34.0	30.0	34.0
4	35.704979544036185	37.0	35.0	37.0	33.0	37.0
5	35.86640830257876	37.0	35.0	37.0	35.0	37.0
6	36.115104283039656	37.0	35.0	37.0	35.0	37.0
7	36.169853841945894	37.0	36.0	37.0	35.0	37.0
8	36.17164518341025	37.0	37.0	37.0	35.0	37.0
9	37.933312017011964	39.0	38.0	39.0	35.0	39.0
10-11	37.86794153677836	39.0	38.0	39.0	35.0	39.0
12-13	37.917542433604794	39.0	38.0	39.0	35.0	39.0
14-15	38.899781186677046	40.0	38.0	41.0	35.5	41.0
16-17	39.16398864328035	40.0	39.0	41.0	36.0	41.0
18-19	39.386796850320906	41.0	39.0	41.0	36.0	41.0
20-21	39.386781440931955	41.0	39.0	41.0	36.0	41.0
22-23	39.30956306677659	40.5	39.0	41.0	36.0	41.0
24-25	39.18362405713801	40.5	39.0	41.0	36.0	41.0
26-27	38.85588946845313	40.0	38.5	41.0	35.0	41.0
28-29	38.92085930457428	40.0	38.5	41.0	36.0	41.0
30-31	39.04140888043085	40.0	39.0	41.0	36.0	41.0
32-33	38.89856191877711	40.0	38.0	41.0	35.0	41.0
34-35	38.51742994506553	40.0	38.0	41.0	34.5	41.0
36-37	38.32142829626091	40.0	37.5	41.0	34.0	41.0
38-39	38.2822036967124	40.0	38.0	41.0	34.0	41.0
40-41	38.57480680478616	40.0	38.0	41.0	35.0	41.0
42-43	38.50305876370473	40.0	38.0	41.0	34.0	41.0
44-45	38.300134446918506	40.0	38.0	41.0	33.0	41.0
46-47	38.05348021049225	40.0	38.0	41.0	33.0	41.0
48-49	37.71919085298673	40.0	37.0	41.0	32.5	41.0
50-51	37.322612893035725	39.0	37.0	41.0	32.0	41.0
52-53	37.656447673567506	40.0	37.0	41.0	32.5	41.0
54-55	37.58179688884437	40.0	37.5	41.0	31.5	41.0
56-57	37.060077355132485	39.5	36.5	41.0	31.0	41.0
58-59	36.791370356958495	39.0	36.0	40.0	31.0	41.0
60-61	35.99028630644652	38.0	34.5	40.0	28.5	41.0
62-63	36.227785439668395	38.0	35.0	40.0	29.5	41.0
64-65	35.993689855228794	37.5	35.0	39.5	29.5	40.5
66-67	35.30740382615127	37.0	34.5	39.0	28.5	40.0
68-69	34.53952508263285	36.0	34.0	39.0	27.5	39.0
70-71	33.716444129408046	35.0	33.0	37.5	26.5	39.0
72-73	33.09487946005501	35.0	32.0	37.0	26.0	37.5
74-75	32.983436833062385	35.0	32.0	36.5	26.0	37.0
76-77	32.865955651778634	35.0	33.0	36.0	26.0	36.5
78-79	32.49280574153832	35.0	32.0	35.5	26.0	36.0
80-81	31.91292924779068	34.0	31.0	35.0	25.0	36.0
82-83	31.49337011040827	34.0	30.5	35.0	24.0	35.5
84-85	31.37904400151012	34.0	31.0	35.0	24.5	35.0
86-87	31.743805425645846	34.0	31.5	35.0	25.0	35.5
88-89	31.574955120154712	34.0	31.0	35.0	24.5	35.0
90-91	31.207602992503332	34.0	31.0	35.0	23.0	35.0
92-93	31.034470803060305	34.0	31.0	35.0	21.0	35.0
94-95	30.867822114014068	34.0	30.5	35.0	21.5	35.0
96-97	30.65247012504719	34.0	29.5	35.0	19.5	35.0
98-99	30.886544521576997	34.0	31.0	35.0	20.0	35.0
100	29.7489155642533	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.40199531321820103
1101	2	0.3611880057084065
1101	3	0.3650531695392729
1101	4	0.3398245086121463
1101	5	0.26557871012879275
1101	6	0.3459737050408691
1101	7	0.418004221680242
1101	8	0.3586661314054709
1101	9	0.33407777015979434
1101	10-11	0.46420137308577836
1101	12-13	0.4148449619060415
1101	14-15	0.5154497143152525
1101	16-17	0.5123112583125646
1101	18-19	0.615418076028682
1101	20-21	0.36968546227730315
1101	22-23	0.4534901745322131
1101	24-25	0.5260557233312113
1101	26-27	0.4661210795987074
1101	28-29	0.48275705473266584
1101	30-31	0.6565760635574307
1101	32-33	0.5193832176033695
1101	34-35	0.7255576191475512
1101	36-37	0.7174169442344862
1101	38-39	0.749541340192259
1101	40-41	0.6172371396154333
1101	42-43	0.5035463951840811
1101	44-45	0.6157384167166455
1101	46-47	0.7337496619669963
1101	48-49	0.9257598532441094
1101	50-51	0.9483778502078621
1101	52-53	1.1649566904476956
1101	54-55	0.97764993222453
1101	56-57	0.9741837671548836
1101	58-59	1.171590446514557
1101	60-61	1.1832929569273247
1101	62-63	1.1939102657829537
1101	64-65	1.1124492658762293
1101	66-67	0.9499681592770486
1101	68-69	0.9425808558591768
1101	70-71	1.0201284724677677
1101	72-73	0.9652905051469887
1101	74-75	1.081043688190519
1101	76-77	1.172911729538093
1101	78-79	1.025828286860957
1101	80-81	0.8976436706357696
1101	82-83	1.2208519661831971
1101	84-85	0.9809893812370838
1101	86-87	1.2875169435650164
1101	88-89	1.2045309223047518
1101	90-91	1.0494824994031084
1101	92-93	1.3840134029533182
1101	94-95	1.4569310632703107
1101	96-97	1.33115698407207
1101	98-99	1.2114754093043878
1101	100	1.2525306085449692
1102	1	0.48517411489424944
1102	2	0.42811928317888004
1102	3	0.5237328510593926
1102	4	0.33170469199848185
1102	5	0.3924972042145072
1102	6	0.47021715750167203
1102	7	0.35448184681143147
1102	8	0.35363957826368164
1102	9	0.5483967392823956
1102	10-11	0.3761854017458077
1102	12-13	0.4387980263674436
1102	14-15	0.7362905769569963
1102	16-17	0.7885231152964707
1102	18-19	0.6466032220512332
1102	20-21	0.6475544246925722
1102	22-23	0.572718910318585
1102	24-25	0.6851299537635569
1102	26-27	0.6300403706732212
1102	28-29	0.7444613482139317
1102	30-31	0.5028558618640275
1102	32-33	0.5080241544203119
1102	34-35	0.6575097682554727
1102	36-37	0.7237691817213658
1102	38-39	0.6282727865296565
1102	40-41	0.6162783857587115
1102	42-43	0.734187852805583
1102	44-45	0.818284046298345
1102	46-47	0.5488482597401685
1102	48-49	0.9139391678337461
1102	50-51	0.5830216119950151
1102	52-53	0.46778087156142334
1102	54-55	0.6678540718317123
1102	56-57	0.6728154715272723
1102	58-59	0.7347853457862215
1102	60-61	0.8606860933544453
1102	62-63	0.6739816002262842
1102	64-65	0.48542424358421243
1102	66-67	0.5514421940131342
1102	68-69	0.39368835465579366
1102	70-71	0.6787095167598309
1102	72-73	0.7905526828991611
1102	74-75	0.8856275574527857
1102	76-77	0.7086539154968889
1102	78-79	0.5402529950920325
1102	80-81	0.859790647996558
1102	82-83	0.7733624809117075
1102	84-85	0.5463740269716162
1102	86-87	0.8042300354870235
1102	88-89	1.086979019395347
1102	90-91	0.7838642807125353
1102	92-93	0.6421036283985693
1102	94-95	0.7855074518852057
1102	96-97	0.5846467692007344
1102	98-99	0.548680582629725
1102	100	0.38482285881523737
1103	1	0.46503106322974475
1103	2	0.33960410937267227
1103	3	0.31945382205778117
1103	4	0.1352199440605233
1103	5	0.20645961011186387
1103	6	0.3696505852637131
1103	7	0.28875477185799525
1103	8	0.35421084585167506
1103	9	0.5444995987505692
1103	10-11	0.4449605414324722
1103	12-13	0.46877392357058056
1103	14-15	0.4982441555962822
1103	16-17	0.6283295103632298
1103	18-19	0.5504894067663031
1103	20-21	0.5166637167902977
1103	22-23	0.6267115229678595
1103	24-25	0.7288827905375399
1103	26-27	0.5629946849173493
1103	28-29	0.4711802376071361
1103	30-31	0.5443705817863957
1103	32-33	0.7590375674702514
1103	34-35	0.9058350934084629
1103	36-37	0.946363988664217
1103	38-39	0.8003808421852128
1103	40-41	0.8283399574488826
1103	42-43	0.9401259054874131
1103	44-45	0.6302431028460163
1103	46-47	0.7727968926107422
1103	48-49	1.0503077841140893
1103	50-51	0.9821772315464514
1103	52-53	0.8170327457886515
1103	54-55	0.966403991228205
1103	56-57	1.0458453809070178
1103	58-59	1.0116349224221608
1103	60-61	0.882022546487029
1103	62-63	1.3424390212270154
1103	64-65	1.2178910239826948
1103	66-67	1.268586482119808
1103	68-69	1.031336453821595
1103	70-71	1.1755440682756415
1103	72-73	1.1333601891987612
1103	74-75	0.788891608287777
1103	76-77	0.827446271467025
1103	78-79	0.9594029802234232
1103	80-81	0.9754918985247905
1103	82-83	1.3312991875931957
1103	84-85	1.084468966949391
1103	86-87	1.179920643221834
1103	88-89	0.8488621677840591
1103	90-91	0.9055090618309656
1103	92-93	0.8838672018729703
1103	94-95	1.2739373268323817
1103	96-97	0.8967897269582359
1103	98-99	0.7100480250725631
1103	100	0.7087472323772843
1104	1	-0.00479946628791339
1104	2	-0.010122751388394136
1104	3	0.11366823636034695
1104	4	1.2465571239062E-4
1104	5	0.07007622194376495
1104	6	0.0803247425670861
1104	7	0.09437585324106834
1104	8	0.24802796671578875
1104	9	0.09201961861523955
1104	10-11	0.12421592662087022
1104	12-13	0.19006326493254733
1104	14-15	0.23659693975420737
1104	16-17	0.26652865369925394
1104	18-19	0.15756464421121308
1104	20-21	0.19214015853093258
1104	22-23	0.36302352247124503
1104	24-25	0.4237428066775806
1104	26-27	0.5782889303701992
1104	28-29	0.4116638182142438
1104	30-31	0.399886132099887
1104	32-33	0.47934143487356806
1104	34-35	0.48044400767999207
1104	36-37	0.5421955114154784
1104	38-39	0.3017775769297586
1104	40-41	0.25791348777354983
1104	42-43	0.17477734257226984
1104	44-45	0.15014657295588307
1104	46-47	0.5774712982225125
1104	48-49	0.5210229123033017
1104	50-51	0.32131218716132537
1104	52-53	0.3642501397919986
1104	54-55	0.27419777110280563
1104	56-57	0.5909211149687223
1104	58-59	0.6474440974175053
1104	60-61	0.6961016327121214
1104	62-63	0.16787821670780545
1104	64-65	0.43921916353200885
1104	66-67	0.515024808521197
1104	68-69	0.624801287144578
1104	70-71	0.29467778234852204
1104	72-73	0.3842758264201862
1104	74-75	0.3682882188781349
1104	76-77	0.3205479537564244
1104	78-79	0.11865402001484426
1104	80-81	0.2314047422767267
1104	82-83	0.2574196792438208
1104	84-85	0.33784440924872783
1104	86-87	-0.06093850383881971
1104	88-89	-0.22313174866016183
1104	90-91	0.23661279266466195
1104	92-93	0.3771395961867121
1104	94-95	0.24553705369314116
1104	96-97	-0.16535720953689648
1104	98-99	0.002252693270460071
1104	100	0.10042269329975184
1105	1	-0.025558944747331225
1105	2	0.02143706354881658
1105	3	0.06683172851921171
1105	4	0.14641739139706544
1105	5	0.04363868063510523
1105	6	0.12633507724232373
1105	7	0.09730501264742486
1105	8	0.05635579222801823
1105	9	0.1305042668156844
1105	10-11	0.22803585091337197
1105	12-13	0.15043699563014457
1105	14-15	0.08592548629029295
1105	16-17	0.3612231198411706
1105	18-19	0.2824745162294775
1105	20-21	0.3577353036369857
1105	22-23	0.3853902832715761
1105	24-25	0.3512348453725309
1105	26-27	0.3132901320766237
1105	28-29	0.3852863622267435
1105	30-31	0.38895060364948364
1105	32-33	0.5575499910233006
1105	34-35	0.5445475346883413
1105	36-37	0.34814996674204224
1105	38-39	0.4875613899442399
1105	40-41	0.40225345052065364
1105	42-43	0.3931424209836152
1105	44-45	0.385132753332023
1105	46-47	0.40133117925357453
1105	48-49	0.5806575935506757
1105	50-51	0.5558026636379196
1105	52-53	0.5225974930336008
1105	54-55	0.5101227846220127
1105	56-57	0.43463168397671126
1105	58-59	0.6354420545165809
1105	60-61	0.5541500614809749
1105	62-63	0.767003975284986
1105	64-65	0.5826353144663372
1105	66-67	0.39301405324871297
1105	68-69	0.35741409733505236
1105	70-71	0.5490114361369081
1105	72-73	0.48990582101250624
1105	74-75	0.3529814833136342
1105	76-77	0.5117018923492296
1105	78-79	0.7337699846846704
1105	80-81	0.5511337574783077
1105	82-83	0.4946740804935956
1105	84-85	0.5566526169035946
1105	86-87	0.6879198750592153
1105	88-89	0.6236206397313531
1105	90-91	0.5171511571422194
1105	92-93	0.8498833923773006
1105	94-95	1.0632982746268702
1105	96-97	0.41483416221099034
1105	98-99	0.623016678128959
1105	100	0.43903352067462365
1106	1	0.05710980964818191
1106	2	0.02472125563755867
1106	3	0.20433653869954327
1106	4	0.07932698654575177
1106	5	0.1740575975815375
1106	6	0.18478706169051407
1106	7	0.12215208208652939
1106	8	0.13123537185143164
1106	9	0.02357229935240923
1106	10-11	0.03949668609337209
1106	12-13	0.0095352029256901
1106	14-15	0.36404347835514983
1106	16-17	0.2632944830131052
1106	18-19	0.18226353176801524
1106	20-21	0.26883321900434964
1106	22-23	0.16777440768606056
1106	24-25	0.16387998012392302
1106	26-27	0.2709207367660511
1106	28-29	0.19996504218057254
1106	30-31	0.13034867426956254
1106	32-33	0.21848535546910597
1106	34-35	0.29293529493568116
1106	36-37	0.28496741873975395
1106	38-39	0.3291704450257882
1106	40-41	0.32686963130381486
1106	42-43	0.09563774349791032
1106	44-45	0.11741802024727122
1106	46-47	-0.18854621090844148
1106	48-49	-0.07440603352434039
1106	50-51	0.2257612629134087
1106	52-53	0.3438379224334227
1106	54-55	0.29888620336645033
1106	56-57	0.29091344775373784
1106	58-59	0.09320203647011738
1106	60-61	0.26713489448477645
1106	62-63	0.07709142104605604
1106	64-65	0.040882949184556594
1106	66-67	0.18400674177460985
1106	68-69	0.09617817955263774
1106	70-71	-0.02415638276146126
1106	72-73	0.2386684435891695
1106	74-75	0.11504010780110008
1106	76-77	0.24398384189873212
1106	78-79	0.21770398237578803
1106	80-81	0.33558128338694004
1106	82-83	0.5121698674390984
1106	84-85	0.4269966940787917
1106	86-87	0.4282995143952135
1106	88-89	0.39055910923149284
1106	90-91	0.2974486585023399
1106	92-93	0.19556948996093126
1106	94-95	0.17800283003351325
1106	96-97	0.2698652791016869
1106	98-99	0.15627658710044656
1106	100	0.18356318455912657
1107	1	0.15213317501704893
1107	2	0.31764488677963243
1107	3	0.09031227902275418
1107	4	0.11703277183627137
1107	5	0.05573184277305643
1107	6	0.23005667663426976
1107	7	0.16454353703733915
1107	8	0.17892969488552524
1107	9	0.09638281943654903
1107	10-11	0.026992120084628368
1107	12-13	0.1244726643507903
1107	14-15	0.12755256628703648
1107	16-17	0.1827437908691536
1107	18-19	0.15082734882536641
1107	20-21	0.13932546623640718
1107	22-23	0.2789677419430987
1107	24-25	0.02547953171038131
1107	26-27	0.06289652747280883
1107	28-29	0.09851147691498596
1107	30-31	0.1496865085328949
1107	32-33	0.10053432827458408
1107	34-35	-0.0019249537071033274
1107	36-37	0.237940677673393
1107	38-39	0.2137008074094311
1107	40-41	0.19521742891311789
1107	42-43	0.2750881849837228
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2216	92-93	-2.8943808822475035
2216	94-95	-3.163099615907644
2216	96-97	-2.8624459580482586
2216	98-99	-2.834418201981439
2216	100	-3.0798185949516856
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	15.0
11	10.0
12	11.0
13	18.0
14	21.0
15	38.0
16	77.0
17	160.0
18	239.0
19	335.0
20	491.0
21	758.0
22	1012.0
23	1425.0
24	1684.0
25	1987.0
26	2387.0
27	2889.0
28	3481.0
29	4103.0
30	5048.0
31	6151.0
32	7675.0
33	10121.0
34	13802.0
35	21962.0
36	42280.0
37	69597.0
38	56105.0
39	5661.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.664280706079964	14.934854800557837	12.127948099578541	17.27291639378366
2	11.7236198328004	32.92098470547444	24.948568786839772	30.40682667488539
3	40.57577403585008	16.988916754307905	30.795782433999406	11.639526775842608
4	9.975448725607713	12.25636618013775	48.257322022531675	29.510863071722866
5	8.662069496879575	48.39587025194545	32.25209954541952	10.689960705755452
6	23.800809797704677	35.95008687411151	14.485824687657711	25.7632786405261
7	25.637370850058943	31.695186877364378	23.732385142267184	18.9350571303095
8	21.193769960051007	16.669170702015943	18.527164931177552	43.6098944067555
9	13.916272762644416	29.38735885908445	32.01505503099225	24.681313347278884
10-11	29.922336679738965	16.8890755137105	27.30408888135541	25.88449892519512
12-13	41.50827098951391	18.16863264787235	21.331409727947236	18.9916866346665
14-15	24.386706320160876	24.677943771139756	36.845967748148944	14.089382160550423
16-17	18.338521160943362	25.436470941744805	33.878119438173684	22.346888459138153
18-19	14.918657374769339	43.049375724725614	21.98620083904446	20.045766061460586
20-21	23.54944400891435	24.55490857399314	18.667932807230102	33.22771460986241
22-23	17.96542133121711	27.56200352875007	23.056876054580055	31.415699085452765
24-25	31.043562342535306	26.4153523742016	19.71149771555809	22.829587567705005
26-27	26.796401130280856	15.713369404214092	37.61554656244763	19.874682903057423
28-29	16.29735497838833	40.50916473407247	24.318519774098355	18.87496051344084
30-31	22.91202779853765	22.147336872356327	23.413410791195073	31.527224537910946
32-33	25.683406399519228	25.15024154217165	27.804894021927563	21.361458036381567
34-35	32.156120224052515	19.74886548373924	27.433142513733618	20.661871778474623
36-37	26.310568529404964	16.52695487360449	37.39800910694886	19.76446749004168
38-39	20.821206441907457	29.34319279301491	31.933516063355828	17.90208470172181
40-41	20.42730317838521	32.46417791714741	24.35323780777906	22.755281096688325
42-43	18.062885716266923	30.89062415729904	17.742370426300745	33.30411970013329
44-45	24.551968564604362	21.244510362893905	20.363279143231374	33.84024192927036
46-47	31.265072308557606	29.50416438736122	23.83023476204051	15.400528542040664
48-49	27.19891980183526	25.939780108019818	26.161867926127393	20.699432164017537
50-51	12.179581018714703	30.169079520151627	37.739134454623205	19.912205006510465
52-53	22.918576788837438	32.77865953725605	21.5600465363546	22.74271713755191
54-55	17.022559345409157	23.381243691781403	26.727007265526886	32.86918969728255
56-57	28.855814347682042	17.249655214922452	27.477829741661598	26.416700695733912
58-59	39.64203782641608	19.12951858864591	21.87375399924108	19.35468958569692
60-61	23.892349594153657	25.138203489469568	36.76732888770749	14.202118028669277
62-63	18.444075475187034	25.227288486875054	33.57648064965984	22.752155388278076
64-65	15.127204505705327	42.1579693507254	22.016357066360534	20.698469077208742
66-67	23.350842420144886	24.625018539599623	18.729907407942463	33.29423163231303
68-69	18.104577429520223	27.430290702601916	23.229626547704353	31.235505320173512
70-71	30.474906338184166	26.31795899105277	19.91620999508817	23.290924675674894
72-73	26.30954444765482	16.21419628238467	36.82172782432823	20.654531445632283
74-75	16.583101385905945	39.61283239109707	24.093960377921817	19.71010584507517
76-77	22.751464415803554	22.327501353176064	23.27270205663499	31.64833217438539
78-79	25.375414130518532	25.117882733646663	27.30680329763464	22.199899838200167
80-81	31.207925063997244	19.975999830496395	27.084942493975884	21.731132611530484
82-83	25.593646708939755	17.261212256627964	36.322241141527535	20.822899892904747
84-85	20.50935833254681	28.928294196620328	31.261472793708332	19.300874677124526
86-87	20.14430892742948	31.934802875391977	24.340670770700587	23.580217426477954
88-89	18.116048108112274	30.55123236588053	17.954249524235117	33.37847000177208
90-91	24.112033962293225	21.411153315715264	20.441517516622877	34.035295205368634
92-93	30.10262653034494	29.18018198488339	23.734696550608287	16.982494934163388
94-95	26.241033661810143	25.80648889368292	25.934194204528822	22.018283239978118
96-97	12.43154847321554	29.427944595435346	36.79711381589212	21.343393115456998
98-99	22.559730643881316	31.699231841961307	22.06008120747972	23.680956306677658
100	21.235559942625354	15.015346175794686	20.633280380029923	43.11581350155004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	8.0
2	14.0
3	23.0
4	13.5
5	10.0
6	19.5
7	48.0
8	77.0
9	81.5
10	65.5
11	45.5
12	38.0
13	37.5
14	49.5
15	57.0
16	59.5
17	72.0
18	76.5
19	84.0
20	108.0
21	137.5
22	171.5
23	197.5
24	218.5
25	265.5
26	331.0
27	388.5
28	441.0
29	534.0
30	674.0
31	777.0
32	863.5
33	1000.0
34	1131.5
35	1219.5
36	1307.5
37	1450.5
38	1569.5
39	1553.5
40	1551.5
41	1592.5
42	1629.5
43	1727.0
44	1898.0
45	2394.5
46	4273.0
47	23550.0
48	70015.0
49	73759.0
50	32284.0
51	10964.5
52	4720.5
53	2697.5
54	1964.5
55	1601.0
56	1343.0
57	1171.5
58	1031.0
59	919.0
60	774.0
61	592.0
62	433.5
63	355.0
64	281.0
65	199.5
66	148.5
67	111.5
68	78.5
69	65.0
70	52.0
71	45.0
72	35.0
73	26.0
74	24.5
75	17.5
76	12.5
77	8.0
78	2.5
79	2.5
80	3.5
81	2.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003081877788136311
2	0.0046228166822044675
3	3.852347235170389E-4
4	0.048154340439629864
5	7.704694470340778E-4
6	0.004237581958687428
7	0.0
8	3.852347235170389E-4
9	3.852347235170389E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.852347235170389E-4
20-21	1.9261736175851945E-4
22-23	0.0
24-25	0.0
26-27	1.9261736175851945E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.9261736175851945E-4
40-41	1.9261736175851945E-4
42-43	0.0
44-45	7.704694470340778E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.778520852755584E-4
60-61	3.852347235170389E-4
62-63	0.0
64-65	0.0
66-67	9.630868087925973E-4
68-69	0.0015409388940681556
70-71	0.001733556255826675
72-73	0.0026966430646192725
74-75	0.001733556255826675
76-77	0.0028892604263777923
78-79	7.704694470340778E-4
80-81	1.9261736175851945E-4
82-83	0.0
84-85	1.9261736175851945E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	1.9261736175851945E-4
98-99	0.0
100	0.09014492530298711
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	259582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.474909662457335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97901157054444	18.46391506344816
2	7.722665709929141	3.316870969481705
3	1.9912099739886984	1.2828316293117397
4	1.0404520584805812	0.8937445585595304
5	0.6081262893533053	0.6529728563613809
6	0.4538523634406673	0.5847863102988651
7	0.270876311776841	0.4071931027575102
8	0.24934971746344964	0.42838101255094735
9	0.20270876311776842	0.39178371381682864
>10	1.14270338146919	4.826605850944981
>50	0.14889227733429009	2.2320499880577236
>100	0.12377791730200018	5.188726491050997
>500	0.017938828594492782	2.7906403371574298
>1k	0.03408377432953628	20.609672473438064
>5k	0.007175531437797112	9.404350070497955
>10k+	0.007175531437797112	28.525475572266178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	30932	11.91608046782905	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	20469	7.88536955567027	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	12408	4.779992449399419	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10238	3.944033099367444	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	7890	3.039501968549437	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	6187	2.38344723439992	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	5285	2.0359655137875508	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	5050	1.9454353537610467	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4974	1.9161575147737517	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	4614	1.7774730143076174	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4295	1.6545831375056823	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4284	1.6503455555469948	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	4075	1.5698314983319337	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	3901	1.5028006564399687	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	3873	1.4920140841814917	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	3073	1.1838263053678606	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	2903	1.118336402369964	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	2427	0.9349646739758535	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2349	0.9049163655415245	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	2040	0.7858788359747595	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	1975	0.7608385789461519	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	1951	0.7515929455817429	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	1849	0.712299003783005	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1540	0.59326147421624	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1269	0.4888628641431224	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	1096	0.4222172569746747	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1011	0.38947230547572637	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	917	0.3532602414651247	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	889	0.3424736692066476	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	796	0.306646839919563	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	741	0.28545893012612583	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	718	0.27659853148523394	No Hit
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	712	0.27428712314413173	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	673	0.2592629689269672	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	646	0.24886163139200718	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	616	0.23730458968649598	No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	536	0.2064858118051329	No Hit
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	459	0.17682273809432086	No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT	445	0.17142945196508233	No Hit
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	417	0.16064287970660523	No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	416	0.1602576449830882	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	393	0.1513972463421963	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	382	0.14715966438350886	No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	341	0.13136504071931027	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACGCAGTGGTATCAACGCAGAGT	333	0.12828316293117395	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	310	0.11942276429028208	No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT	303	0.1167261212256628	No Hit
AGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	291	0.11210330454345832	No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG	287	0.11056236564939018	No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	268	0.10324290590256645	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC	263	0.10131673228498124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.9261736175851948E-4	0.0
18-19	0.0	0.0	0.0	7.704694470340779E-4	0.0
20-21	0.0	0.0	0.0	0.0011557041705511169	0.0
22-23	0.0	0.0	0.0	0.0013483215323096365	0.0
24-25	0.0	0.0	0.0	0.0015409388940681558	0.0
26-27	0.0	0.0	0.0	0.0019261736175851948	0.0
28-29	0.0	0.0	0.0	0.005393286129238545	0.0
30-31	0.0	0.0	0.0	0.013097980599579322	0.0
32-33	0.0	0.0	0.0	0.030240925796087555	0.0
34-35	0.0	0.0	0.0	0.05508856546293657	0.0
36-37	0.0	0.0	0.0	0.0851368738972656	0.0
38-39	0.0	0.0	0.0	0.13252074488986137	0.0
40-41	0.0	0.0	0.0	0.1789415290736646	0.0
42-43	0.0	0.0	0.0	0.22825157368384558	0.0
44-45	0.0	0.0	0.0	0.2987495280874637	0.0
46-47	0.0	0.0	0.0	0.3944803568814479	0.0
48-49	0.0	0.0	0.0	0.4742239446494749	0.0
50-51	0.0	0.0	0.0	0.5582051143761895	0.0
52-53	0.0	0.0	0.0	0.6435346056352136	0.0
54-55	0.0	0.0	0.0	0.7673875692459415	0.0
56-57	0.0	0.0	0.0	0.980037136627347	0.0
58-59	0.0	0.0	0.0	1.1489625628895686	0.0
60-61	0.0	0.0	0.0	1.300359809231765	0.0
62-63	0.0	0.0	0.0	1.4665885924293671	0.0
64-65	0.0	0.0	0.0	1.6064287970660525	0.0
66-67	0.0	0.0	0.0	1.80058709771864	0.0
68-69	0.0	0.0	0.0	2.0623540923484676	0.0
70-71	0.0	0.0	0.0	2.2938801611822086	0.0
72-73	0.0	0.0	0.0	2.4801411500026966	0.0
74-75	0.0	0.0	0.0	2.7029994375573034	0.0
76-77	0.0	0.0	0.0	2.902165789615613	0.0
78-79	0.0	0.0	0.0	3.3548165897481335	0.0
80-81	0.0	0.0	0.0	3.7635506313997116	0.0
82-83	0.0	0.0	0.0	4.026858564923608	0.0
84-85	0.0	0.0	0.0	4.3348537263754805	0.0
86-87	0.0	0.0	0.0	4.615689839819402	0.0
88	0.0	0.0	0.0	4.875915895555162	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	25	5.5263292E-5	75.26834	3
TAGTAGT	20	0.0021533149	70.564064	4
TAAAGCT	25	0.0052127084	56.451256	4
CTCTTAC	25	0.0052127084	56.451256	1
ACCCAGT	25	0.0052127084	56.451256	3
GTACCCA	60	2.5274858E-8	54.88316	1
GAGTACG	945	0.0	54.75871	1
TACGCAG	960	0.0	53.903107	4
GTACGCA	960	0.0	53.903107	3
CGCAGTG	960	0.0	53.86156	6
AGTACGC	985	0.0	52.53501	2
ACGCAGT	995	0.0	51.96693	5
ACCCATG	40	5.6432193E-4	47.04271	3
TACCCAT	45	0.0010079867	41.815742	2
TACCTGG	125	3.6379788E-12	41.397583	2
GTACCTG	140	1.2732926E-11	36.962128	1
ACCTGGG	130	2.2555469E-10	36.186703	3
AGTATGC	55	0.002701092	34.21288	94
CCAGTGG	60	0.00415231	31.337635	5
AGTAGGC	305	0.0	30.847677	2
>>END_MODULE