FastQCFastQC Report
Mon 16 Jan 2017
ERR1146605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041396
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA337731.110444019785651No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA279750.9198078776982674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG28400.020.6906642
GTACCCG5100.020.2828141
GTATTAG9150.017.9855061
TATACTC25000.017.8631974
GTATAAT16500.017.097911
TATACTG14100.016.0026025
ACCTGGG35300.015.8472913
GCTTATA29100.015.8346631
GTACTAG8350.015.7669351
GTACCTG39900.015.6730841
ATACTCT27100.015.4379355
GTATAGG10050.014.9713041
TAGCCCT22000.014.9572244
ATAGGAC8200.014.90535453
TACGGGC4101.22763595E-814.9051094
TGCGCGA2902.0308275E-514.60998294
GTACATG179650.014.44732951
CATGGGG47350.014.3953974
TACATGG174450.014.3897442
GTCCTAC8900.014.2642671
Produced by FastQC (version 0.11.5)