##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146605_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3041396
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.629136094083112	31.0	31.0	34.0	27.0	34.0
2	30.690477991027805	31.0	31.0	34.0	27.0	34.0
3	30.890348379494153	31.0	31.0	34.0	27.0	34.0
4	34.38922192309058	37.0	35.0	37.0	30.0	37.0
5	34.462415285612266	37.0	35.0	37.0	30.0	37.0
6	34.39363239775419	37.0	35.0	37.0	30.0	37.0
7	34.39698875121819	37.0	35.0	37.0	30.0	37.0
8	34.418434166415686	37.0	35.0	37.0	30.0	37.0
9	35.99707075303578	39.0	37.0	39.0	30.0	39.0
10-11	36.00734103681336	39.0	37.0	39.0	30.0	39.0
12-13	35.96323629017728	39.0	37.0	39.0	30.0	39.0
14-15	37.14113009289156	40.0	37.0	41.0	30.0	41.0
16-17	37.10534833346266	40.0	37.0	41.0	30.0	41.0
18-19	37.11290818426801	40.0	37.0	41.0	30.0	41.0
20-21	37.22683119856803	40.0	37.0	41.0	30.0	41.0
22-23	37.192917331383356	40.0	37.0	41.0	30.0	41.0
24-25	37.19504612355642	40.0	37.0	41.0	30.0	41.0
26-27	37.209063370899415	40.0	37.0	41.0	30.0	41.0
28-29	37.14387044633451	40.0	37.0	41.0	30.0	41.0
30-31	37.11276778821305	40.0	37.0	41.0	30.0	41.0
32-33	37.09588524480206	40.0	37.0	41.0	30.0	41.0
34-35	37.031537655734404	40.0	37.0	41.0	30.0	41.0
36-37	36.91071682214351	40.0	37.0	41.0	30.0	41.0
38-39	36.813442083832555	40.0	36.0	41.0	29.0	41.0
40-41	36.74394916018828	40.0	36.0	41.0	29.0	41.0
42-43	36.589376062834305	40.0	36.0	41.0	28.5	41.0
44-45	36.4694375543336	39.5	35.5	41.0	28.0	41.0
46-47	36.344786242896355	39.0	35.0	41.0	28.0	41.0
48-49	36.216292781341195	39.0	35.0	41.0	28.0	41.0
50-51	36.07264788932451	39.0	35.0	40.5	27.0	41.0
52-53	35.90924200597357	39.0	35.0	40.0	27.0	41.0
54-55	35.81258902162033	39.0	35.0	40.0	27.0	41.0
56-57	35.66615577188897	38.5	34.0	40.0	27.0	41.0
58-59	35.50674114781501	38.0	34.0	40.0	26.5	41.0
60-61	35.26512775712206	38.0	34.0	40.0	26.0	41.0
62-63	34.99292972700694	37.5	34.0	40.0	26.0	41.0
64-65	34.628445128487044	37.0	33.0	40.0	26.0	41.0
66-67	34.40669629998855	36.5	33.0	39.5	26.0	41.0
68-69	34.22507526149176	36.0	33.0	39.0	26.0	41.0
70-71	33.89596241331283	36.0	33.0	39.0	25.5	40.5
72-73	33.486061663788604	35.0	32.0	38.5	24.5	40.0
74-75	33.11467793079231	35.0	32.0	37.5	24.0	39.5
76-77	32.79155558828906	35.0	31.5	37.0	24.0	39.0
78-79	32.500590518301465	35.0	31.0	36.5	23.5	39.0
80-81	32.286585337785674	35.0	31.0	36.0	23.5	38.5
82-83	32.050743967572785	35.0	31.0	36.0	23.0	37.0
84-85	31.862948461824768	35.0	31.0	35.0	23.0	37.0
86-87	31.585140014651166	35.0	31.0	35.0	22.5	36.5
88-89	31.341080050082265	34.0	31.0	35.0	21.0	36.0
90-91	31.17466880340475	34.0	30.5	35.0	20.5	36.0
92-93	31.075461235564195	34.0	30.5	35.0	20.0	36.0
94-95	31.02593019784336	34.0	30.5	35.0	20.0	35.0
96-97	30.9190273150882	34.0	30.0	35.0	20.0	35.0
98-99	30.7563756906368	34.0	30.0	35.0	20.0	35.0
100	29.376703000858818	34.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4024036326501452
1101	2	0.47991565922502133
1101	3	0.42199227764513836
1101	4	0.40381448082014515
1101	5	0.4198406155361951
1101	6	0.44686281629238067
1101	7	0.5001623108720921
1101	8	0.5527922431990078
1101	9	0.6657256061956573
1101	10-11	0.6826318799831554
1101	12-13	0.7729107569155715
1101	14-15	0.8444062927228728
1101	16-17	0.7976703221648904
1101	18-19	0.7349851078597922
1101	20-21	0.7741515190610286
1101	22-23	0.8095943309269273
1101	24-25	0.786282963652333
1101	26-27	0.8430691580668821
1101	28-29	0.8015594786025702
1101	30-31	0.8326141895282646
1101	32-33	0.8762655957903434
1101	34-35	0.8097188952584489
1101	36-37	0.8450705216954901
1101	38-39	0.8978156992264985
1101	40-41	0.8693829965967126
1101	42-43	0.9059258311871972
1101	44-45	0.7920245547105651
1101	46-47	0.7226788090022609
1101	48-49	0.7530172117567631
1101	50-51	0.746653908047648
1101	52-53	0.6971687027837987
1101	54-55	0.6580061201230407
1101	56-57	0.7799580048379582
1101	58-59	0.8075092496475591
1101	60-61	0.8314143463329202
1101	62-63	0.7845475894600611
1101	64-65	0.837188041302511
1101	66-67	0.6963169717588116
1101	68-69	0.8000519973464009
1101	70-71	0.7234239861719018
1101	72-73	0.6839305735778751
1101	74-75	0.7118466074355823
1101	76-77	0.7772402719957014
1101	78-79	0.7219840963246327
1101	80-81	0.5958350782116213
1101	82-83	0.6268079018628328
1101	84-85	0.6838252732276651
1101	86-87	0.6681633342256923
1101	88-89	0.6704818654015625
1101	90-91	0.6222391778116325
1101	92-93	0.6631660168612612
1101	94-95	0.6075792907356821
1101	96-97	0.6126181129936121
1101	98-99	0.5872702572083135
1101	100	0.3729215075632304
1102	1	0.3667139247437383
1102	2	0.4222562602149935
1102	3	0.41385923558590676
1102	4	0.459708909767393
1102	5	0.43886231784425433
1102	6	0.42121678982218214
1102	7	0.393883963429964
1102	8	0.50008027740855
1102	9	0.5593299005851122
1102	10-11	0.5675704230189993
1102	12-13	0.5645640745946992
1102	14-15	0.7083547828918881
1102	16-17	0.7159520146777751
1102	18-19	0.6626688834922732
1102	20-21	0.6536585566795239
1102	22-23	0.5117970106615672
1102	24-25	0.6454721566346535
1102	26-27	0.5804962266195375
1102	28-29	0.6018663278672562
1102	30-31	0.6783223166177237
1102	32-33	0.6939023749208175
1102	34-35	0.6251978569314502
1102	36-37	0.6030813660742069
1102	38-39	0.6599159313295004
1102	40-41	0.6114805572890347
1102	42-43	0.6953568449101226
1102	44-45	0.5685996465161054
1102	46-47	0.546131834762015
1102	48-49	0.5733954535039487
1102	50-51	0.6108480034557289
1102	52-53	0.5837437268361754
1102	54-55	0.5447282653432453
1102	56-57	0.5853356948807615
1102	58-59	0.5499769120277094
1102	60-61	0.5377392014863176
1102	62-63	0.46782088057994997
1102	64-65	0.5258646476817646
1102	66-67	0.5220351311568479
1102	68-69	0.4948400605930203
1102	70-71	0.5928015620022151
1102	72-73	0.473532664053522
1102	74-75	0.5360418933636382
1102	76-77	0.47868992364761453
1102	78-79	0.4837006761059257
1102	80-81	0.5174670439633573
1102	82-83	0.5586852357175331
1102	84-85	0.5092948026915813
1102	86-87	0.471086786694908
1102	88-89	0.4700679903267968
1102	90-91	0.43215149069189707
1102	92-93	0.4628918826983721
1102	94-95	0.48056387592173877
1102	96-97	0.4722718991375068
1102	98-99	0.40078655474925995
1102	100	0.4189384923148971
1103	1	0.3451157312705426
1103	2	0.36207158729297717
1103	3	0.2999174350341356
1103	4	0.4632787181235969
1103	5	0.3654806955108967
1103	6	0.40953987891663246
1103	7	0.39558334527175276
1103	8	0.37454731880596626
1103	9	0.47224303570948223
1103	10-11	0.574450475982907
1103	12-13	0.5877059347124316
1103	14-15	0.6400921680015017
1103	16-17	0.6687478343644173
1103	18-19	0.6117465562151168
1103	20-21	0.5359632245854584
1103	22-23	0.5423902636638402
1103	24-25	0.5808013646102239
1103	26-27	0.5278412075879331
1103	28-29	0.5871049651529674
1103	30-31	0.66329205415866
1103	32-33	0.6218305582880816
1103	34-35	0.6733764386345626
1103	36-37	0.5885029197315248
1103	38-39	0.613915172462832
1103	40-41	0.5485106454761635
1103	42-43	0.5998475282773867
1103	44-45	0.5051099156509835
1103	46-47	0.6288189096860322
1103	48-49	0.5624586220073837
1103	50-51	0.5780252914894248
1103	52-53	0.5944789699685629
1103	54-55	0.5828373411303218
1103	56-57	0.5988171040306796
1103	58-59	0.5622650425417177
1103	60-61	0.5404593189216342
1103	62-63	0.5295797638380435
1103	64-65	0.5428459028453787
1103	66-67	0.562022125307216
1103	68-69	0.512821074173317
1103	70-71	0.5481630517782676
1103	72-73	0.4664744203457545
1103	74-75	0.4718090863499711
1103	76-77	0.48313581728456967
1103	78-79	0.4435872425985039
1103	80-81	0.41719832863333295
1103	82-83	0.47446532851503775
1103	84-85	0.43917386350418397
1103	86-87	0.4324747197007177
1103	88-89	0.3973429997319471
1103	90-91	0.5306761036053551
1103	92-93	0.44207895024580424
1103	94-95	0.4155364718911727
1103	96-97	0.4783387983511638
1103	98-99	0.37738397829090786
1103	100	0.29889878782107715
1104	1	0.24299523836682368
1104	2	0.22614667359907514
1104	3	0.20229864427847843
1104	4	0.2847509563284305
1104	5	0.2684276891817774
1104	6	0.3011052557865952
1104	7	0.38934293483830373
1104	8	0.35779914082361586
1104	9	0.5007258464114344
1104	10-11	0.5164971292241844
1104	12-13	0.5008613216514419
1104	14-15	0.537787375615622
1104	16-17	0.5665650092781149
1104	18-19	0.538671362026399
1104	20-21	0.5647367190235144
1104	22-23	0.5334797582936801
1104	24-25	0.5827054929186275
1104	26-27	0.6370112306029014
1104	28-29	0.5935632385515035
1104	30-31	0.6102821289572802
1104	32-33	0.5841165910843955
1104	34-35	0.5008019996242012
1104	36-37	0.5320934931876025
1104	38-39	0.5091236856005423
1104	40-41	0.49768264633922144
1104	42-43	0.5897137538239932
1104	44-45	0.5462308760460672
1104	46-47	0.5241436963181769
1104	48-49	0.5442771034995246
1104	50-51	0.4734885274886551
1104	52-53	0.4920468161519125
1104	54-55	0.4100818990513062
1104	56-57	0.497170695323355
1104	58-59	0.5390160374592412
1104	60-61	0.48372264841913903
1104	62-63	0.4026420720467101
1104	64-65	0.44186447064361545
1104	66-67	0.42215020595546804
1104	68-69	0.44332638439579597
1104	70-71	0.5338434315251064
1104	72-73	0.45855523641786533
1104	74-75	0.5039227466875289
1104	76-77	0.40642737606861346
1104	78-79	0.4092362165148842
1104	80-81	0.4386828534703042
1104	82-83	0.4819967343454934
1104	84-85	0.3751419302475689
1104	86-87	0.3498731901648533
1104	88-89	0.37348925260882027
1104	90-91	0.4447101345796547
1104	92-93	0.4894055588955908
1104	94-95	0.4908984780284946
1104	96-97	0.520862232854423
1104	98-99	0.5960198025141565
1104	100	0.7519206999806478
1105	1	0.1898133399484152
1105	2	0.21255133415430194
1105	3	0.25786067052671413
1105	4	0.28313147136450567
1105	5	0.16655667545200714
1105	6	0.30167255435522833
1105	7	0.3236430414235514
1105	8	0.252591423823759
1105	9	0.3610133936410236
1105	10-11	0.4042227687486246
1105	12-13	0.40517033708637484
1105	14-15	0.5471630987155294
1105	16-17	0.45761640366061584
1105	18-19	0.46279643378638013
1105	20-21	0.43800745228757876
1105	22-23	0.47651995889550847
1105	24-25	0.5102396620583889
1105	26-27	0.43058192447339394
1105	28-29	0.4578469561663425
1105	30-31	0.4928419815704359
1105	32-33	0.4841675542599475
1105	34-35	0.48946783079502154
1105	36-37	0.5116124308344467
1105	38-39	0.5381737309730426
1105	40-41	0.560538023251901
1105	42-43	0.541627422359916
1105	44-45	0.4921534596435393
1105	46-47	0.41758220687860614
1105	48-49	0.471429242943465
1105	50-51	0.4867256235039008
1105	52-53	0.49361008373783477
1105	54-55	0.49870624014067033
1105	56-57	0.47799699760202685
1105	58-59	0.47745901797417645
1105	60-61	0.49597858036194253
1105	62-63	0.5015569368842918
1105	64-65	0.536112801334319
1105	66-67	0.41740972473264293
1105	68-69	0.42810961877930254
1105	70-71	0.4230927638790334
1105	72-73	0.41155688722098205
1105	74-75	0.5272429160436687
1105	76-77	0.4977897260585493
1105	78-79	0.4837056585392929
1105	80-81	0.3866504088995555
1105	82-83	0.4345756677372208
1105	84-85	0.4049206532641385
1105	86-87	0.43120027229140234
1105	88-89	0.3735625267327265
1105	90-91	0.3093537262385695
1105	92-93	0.42031414250237376
1105	94-95	0.43422155143151286
1105	96-97	0.41865878081752683
1105	98-99	0.4097734409846474
1105	100	0.5694222154168749
1106	1	0.14801803405096692
1106	2	0.1564377612905865
1106	3	0.15375154375562872
1106	4	0.15756188717762853
1106	5	0.12830356638027496
1106	6	0.0801769276128681
1106	7	0.22945210586632925
1106	8	0.19053044514401307
1106	9	0.31201579463931495
1106	10-11	0.29174245499973495
1106	12-13	0.3165030309956336
1106	14-15	0.35310465968623816
1106	16-17	0.27348126883166657
1106	18-19	0.3616731908616728
1106	20-21	0.33957306378422203
1106	22-23	0.2959485756391729
1106	24-25	0.3863505711564059
1106	26-27	0.39200877753749097
1106	28-29	0.2957446074107324
1106	30-31	0.3008431487349128
1106	32-33	0.29306339380279667
1106	34-35	0.27524031432594853
1106	36-37	0.3027701673694807
1106	38-39	0.29209677010012314
1106	40-41	0.2695496919893259
1106	42-43	0.2935149665333583
1106	44-45	0.3099425371214224
1106	46-47	0.3458692130455745
1106	48-49	0.29499880226718744
1106	50-51	0.4019374951366643
1106	52-53	0.3038969814292898
1106	54-55	0.2154699232193238
1106	56-57	0.26713548043456825
1106	58-59	0.32644613535360634
1106	60-61	0.34184978363112606
1106	62-63	0.29063669556336436
1106	64-65	0.3755121417189571
1106	66-67	0.25301182049567217
1106	68-69	0.26121539915086345
1106	70-71	0.2610488886403104
1106	72-73	0.3078038649196415
1106	74-75	0.2560874582965411
1106	76-77	0.22413060926781014
1106	78-79	0.24570866998806906
1106	80-81	0.2849201742766354
1106	82-83	0.2357056076716617
1106	84-85	0.1908080035451114
1106	86-87	0.259975177673585
1106	88-89	0.23484507327268034
1106	90-91	0.2932476433987645
1106	92-93	0.2795493139142451
1106	94-95	0.28064320278378574
1106	96-97	0.26605062135330115
1106	98-99	0.31324516882619236
1106	100	0.4219374187819014
1107	1	0.021650373690775382
1107	2	0.10750595245351846
1107	3	0.11156776318326678
1107	4	0.08407375903569658
1107	5	0.044444963856157926
1107	6	0.14827698734372063
1107	7	0.17451323817831366
1107	8	0.21146923485594726
1107	9	0.2721897334111816
1107	10-11	0.21094728975263877
1107	12-13	0.2619551745044575
1107	14-15	0.2699499313982088
1107	16-17	0.27816733128598514
1107	18-19	0.26137176365850934
1107	20-21	0.3297243801517098
1107	22-23	0.25602327436315875
1107	24-25	0.2951532199438347
1107	26-27	0.24549069217570718
1107	28-29	0.25028919932960747
1107	30-31	0.3508324774183933
1107	32-33	0.3525601009850732
1107	34-35	0.3548303410772462
1107	36-37	0.31208083572133205
1107	38-39	0.35291808732456076
1107	40-41	0.30683039471407625
1107	42-43	0.32191662259581477
1107	44-45	0.3618623391664144
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2216	76-77	-0.838527248629056
2216	78-79	-0.7397784124550242
2216	80-81	-0.6729910369536789
2216	82-83	-0.7730923969431416
2216	84-85	-0.7129729791740935
2216	86-87	-0.7828636376754368
2216	88-89	-0.737198003488885
2216	90-91	-0.7914332288660653
2216	92-93	-0.6747058041091236
2216	94-95	-0.7894229274918807
2216	96-97	-0.8245186968856366
2216	98-99	-0.7483323447448385
2216	100	-0.9497397305966331
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	867.0
1	232.0
2	162.0
3	164.0
4	147.0
5	192.0
6	264.0
7	633.0
8	1626.0
9	2805.0
10	4223.0
11	7047.0
12	10486.0
13	13120.0
14	13973.0
15	13964.0
16	14412.0
17	15241.0
18	16035.0
19	17282.0
20	18664.0
21	20194.0
22	21872.0
23	24598.0
24	27948.0
25	32205.0
26	35788.0
27	36850.0
28	41217.0
29	48485.0
30	57174.0
31	67394.0
32	85307.0
33	112524.0
34	159936.0
35	238637.0
36	394896.0
37	656998.0
38	670054.0
39	157499.0
40	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42091801558778	18.59607333883356	13.036555880003805	23.94645276557486
2	14.059659361953422	20.86261633970371	40.53704611477206	24.54067818357081
3	19.439883599185826	26.12101920468657	30.262928433373236	24.176168762754372
4	12.017341930592792	16.8041412151197	39.656932231431064	31.52158462285644
5	13.232144948938561	38.70192465887748	35.06197920162786	13.003951190556101
6	29.326515374138197	37.361431363380134	19.25394484797967	14.058108414501996
7	26.582358872029232	33.370769583118346	22.12943048637948	17.917441058472942
8	24.168890614665223	35.084586806366794	21.229824047596328	19.516698531371652
9	25.888991433925078	17.58231706815516	20.53630505557996	35.9923864423398
10-11	23.593337255536824	25.886789808062105	29.261266792756295	21.25860614364478
12-13	26.517646412408034	23.3789363014333	27.842108215624506	22.261309070534157
14-15	23.823558251363387	25.055147506417974	26.33657023311338	24.78472400910526
16-17	22.14619568713526	28.080411428428526	27.77987545863118	21.993517425805027
18-19	21.829992173642797	29.245255857058506	27.807127759810957	21.117624209487744
20-21	22.695576942912997	28.344774160264365	26.340914433735414	22.618734463087232
22-23	22.29913497826752	27.805282917054647	25.391546543038864	24.50403556163897
24-25	22.748706784932928	27.827378944836532	26.35786348137017	23.066050788860366
26-27	23.746975264660744	26.756156114756763	26.700074408201452	22.796794212381045
28-29	22.648593021333163	26.87600308978389	27.878586958578307	22.59681693030464
30-31	22.657064817418377	28.529233247837187	26.974803783636236	21.838898151108193
32-33	22.182609446791357	28.449921640087368	26.279191638318615	23.08827727480266
34-35	22.68233593763883	27.530839334029654	26.532341235758423	23.25448349257309
36-37	23.74852510929946	27.17922248188379	26.895441252908853	22.176811155907895
38-39	24.309525353081852	26.257880314327338	27.35208275263818	22.080511579952635
40-41	22.820608251632777	26.87689155174621	28.295886490935196	22.00661370568582
42-43	22.490391358590646	27.97627647086506	27.194783508974812	22.338548661569483
44-45	22.51306729639131	28.460536164329962	26.399402261789522	22.626994277489203
46-47	22.267399789329794	27.57133976971068	25.79959741654225	24.361663024417275
48-49	22.959225482617097	27.350984289089443	26.418446193282474	23.271344035010987
50-51	23.760309160454813	27.640430173486457	26.337249615869517	22.26201105018921
52-53	22.318324552536435	27.572749904682954	27.423352668803325	22.685572873977286
54-55	21.903383685353216	27.381187634151622	27.771657799325222	22.943770881169947
56-57	22.756549386602305	28.24160204464802	26.28423200804853	22.717616560701146
58-59	22.191166640721498	27.439682417962523	26.911240204327257	23.457910736988715
60-61	22.512860436556302	26.722786730428695	27.324681983837014	23.439670849177986
62-63	24.078572891957336	26.46427745702788	26.623733627562057	22.833416023452727
64-65	23.21586549727619	26.684822103270413	27.65586204808333	22.443450351370068
66-67	22.401125476141146	26.790090767761605	27.96847376634456	22.84030998975269
68-69	22.206398994480868	28.033897763993615	27.228268015345424	22.531435226180097
70-71	22.057398370799525	27.728234444782164	26.379744477409943	23.834622707008364
72-73	22.1527787034171	27.279388883869863	26.102995928338895	24.46483648437414
74-75	22.643604371690092	27.277774916183162	26.663274442813247	23.4153462693135
76-77	23.251637950356123	26.31370908579875	27.15992721171432	23.27472575213081
78-79	22.427043925379454	26.419920624001463	27.944014840375775	23.209020610243307
80-81	22.009118127874306	27.93009215878523	27.29474585324962	22.766043860090846
82-83	22.08159929536948	27.48412869776327	26.62863522599655	23.805636780870703
84-85	22.4353607023877	26.59874430689045	26.701465479959	24.26442951076285
86-87	22.978521942196068	26.54834665245317	27.315487144415044	23.15764426093572
88-89	23.7737723159739	25.498805388103644	27.61737993693792	23.110042358984536
90-91	22.562176385356594	26.134018116249084	28.25336748471457	23.05043801367975
92-93	21.962356695999787	27.261488549913505	27.5627291041619	23.213425649924808
94-95	21.74993343989532	27.516243462876243	27.04064724395093	23.693175853277502
96-97	21.979427488998876	26.56122348405131	26.4348687162609	25.02448031068892
98-99	22.461041769384654	26.444267003351268	26.79407317492218	24.300618052341903
100	22.212037492987697	27.710835431255713	26.977436045503655	23.09969103025293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1172.0
1	785.0
2	405.0
3	393.0
4	348.5
5	294.5
6	253.5
7	251.5
8	299.5
9	325.0
10	291.0
11	277.0
12	296.0
13	321.5
14	336.5
15	408.5
16	502.0
17	577.0
18	755.5
19	1033.0
20	1447.5
21	2058.0
22	2971.0
23	4184.0
24	5898.0
25	8476.5
26	12103.0
27	16527.0
28	21256.5
29	26825.0
30	33134.5
31	38657.0
32	44198.0
33	50007.5
34	55839.5
35	61768.5
36	68307.0
37	75175.0
38	79709.0
39	82827.0
40	86216.0
41	88940.5
42	91586.5
43	96717.5
44	104405.0
45	111038.0
46	117743.5
47	131422.0
48	203876.5
49	210656.0
50	147037.5
51	128557.0
52	109727.0
53	99179.0
54	92097.0
55	82870.0
56	74108.5
57	65921.5
58	57928.5
59	50636.5
60	42520.0
61	34620.0
62	27786.0
63	21299.5
64	15525.5
65	11294.0
66	8443.5
67	6282.5
68	4784.5
69	3755.5
70	2989.5
71	2382.5
72	1916.5
73	1653.0
74	1358.5
75	1058.0
76	789.0
77	566.0
78	407.0
79	289.5
80	213.5
81	157.5
82	115.0
83	80.0
84	62.5
85	50.0
86	46.5
87	35.0
88	20.0
89	18.0
90	17.5
91	15.0
92	10.0
93	8.5
94	9.0
95	8.0
96	7.5
97	6.0
98	5.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08986005110810957
2	0.05938062652808119
3	0.07371614876852603
4	0.07522861212416929
5	0.07601772344015709
6	0.08413899406719809
7	0.08062087278341919
8	0.08239637324439172
9	0.06842252702377462
10-11	0.07522861212416929
12-13	0.06610451253306047
14-15	0.07476829718984308
16-17	0.07646159855540022
18-19	0.07583688543024322
20-21	0.06778137407953452
22-23	0.07121729626789804
24-25	0.07447238044634766
26-27	0.06840608720469153
28-29	0.07721783023322185
30-31	0.08113050717499463
32-33	0.08330056329396106
34-35	0.08489522574501972
36-37	0.08586517507092137
38-39	0.08722968005481693
40-41	0.08400747551453346
42-43	0.0895970140027803
44-45	0.08573365651825675
46-47	0.0829553270932164
48-49	0.09229314433240525
50-51	0.08926821762111872
52-53	0.09378916786896543
54-55	0.08625973072891528
56-57	0.09301649637206072
58-59	0.09656749729400578
60-61	0.09569618688260259
62-63	0.0967812149420858
64-65	0.09877043305113835
66-67	0.09987190092970466
68-69	0.09750456698174129
70-71	0.09977326201520617
72-73	0.09717577060007969
74-75	0.10108844754185249
76-77	0.09847451630764292
78-79	0.105149082855373
80-81	0.1003815353212801
82-83	0.10547787923703457
84-85	0.10107200772276942
86-87	0.09763608553440592
88-89	0.09737304842907665
90-91	0.09743880770540897
92-93	0.09188214885532828
94-95	0.0917013108454144
96-97	0.08974497237452801
98-99	0.10330782311806816
100	0.24469026723254717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3041396.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.1739467529182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03564411316962	55.462430815801575
2	13.246363947082735	18.590992765847183
3	4.101746199593636	8.635071582128052
4	1.7009285928247846	4.774434900136101
5	0.7782754977577875	2.730733166937794
6	0.40748601596685907	1.7156941192210289
7	0.22707216174334294	1.1154184851073163
8	0.14565071428495754	0.8176708375005691
9	0.08554238380084139	0.5402562017362144
>10	0.26605538865824685	2.7246353296796766
>50	0.003980381153800419	0.18706353316012572
>100	0.001135524668975404	0.12568529605059275
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.1907929437179769E-4	2.579912966693794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	33773	1.110444019785651	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	27975	0.9198078776982674	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2879638166157906E-5
2	0.0	0.0	0.0	0.0	3.2879638166157906E-5
3	0.0	0.0	0.0	0.0	3.2879638166157906E-5
4	0.0	0.0	0.0	1.3151855266463163E-4	3.2879638166157906E-5
5	0.0	0.0	0.0	1.3151855266463163E-4	3.2879638166157906E-5
6	0.0	0.0	0.0	1.3151855266463163E-4	6.575927633231581E-5
7	0.0	0.0	0.0	1.3151855266463163E-4	6.575927633231581E-5
8	0.0	0.0	0.0	1.3151855266463163E-4	9.863891449847373E-5
9	0.0	0.0	0.0	1.9727782899694747E-4	9.863891449847373E-5
10-11	0.0	0.0	0.0	2.3015746716310536E-4	9.863891449847373E-5
12-13	0.0	0.0	0.0	2.6303710532926325E-4	1.1507873358155268E-4
14-15	0.0	0.0	0.0	3.1235656257850013E-4	1.6439819083078955E-4
16-17	0.0	0.0	0.0	3.9455565799389493E-4	1.6439819083078955E-4
18-19	0.0	0.0	0.0	4.767547534092896E-4	1.9727782899694747E-4
20-21	0.0	0.0	0.0	6.247131251570003E-4	2.3015746716310536E-4
22-23	0.0	0.0	0.0	8.384307732370267E-4	3.1235656257850013E-4
24-25	0.0	0.0	0.0	0.0013480651648124743	3.287963816615791E-4
26-27	0.0	0.0	0.0	0.0023508941288802906	3.287963816615791E-4
28-29	0.0	0.0	0.0	0.005211422649336029	3.4523620074465805E-4
30-31	0.0	0.0	0.0	0.012560021779472321	3.61676019827737E-4
32-33	0.0	0.0	0.0	0.029197118691548223	3.61676019827737E-4
34-35	0.0	0.0	0.0	0.06146848355163221	4.274352961600528E-4
36-37	0.0	0.0	0.0	0.11239904307101081	4.274352961600528E-4
38-39	0.0	0.0	0.0	0.1797365420353022	4.603149343262107E-4
40-41	0.0	0.0	0.0	0.26006149807522594	4.931945724923686E-4
42-43	0.0	0.0	0.0	0.33765744414735865	4.931945724923686E-4
44-45	0.0	0.0	0.0	0.43218640387506263	4.931945724923686E-4
46-47	0.0	0.0	0.0	0.5522792822769544	5.096343915754475E-4
48-49	0.0	0.0	0.0	0.6863624467185463	5.918334869908423E-4
50-51	0.0	0.0	0.0	0.8223361903546924	6.247131251570003E-4
52-53	0.0	0.0	0.0	0.9598059575273985	6.904724014893161E-4
54-55	0.0	0.0	0.0	1.1098686261177435	7.562316778216319E-4
56-57	0.0	0.0	0.0	1.285577412477691	7.726714969047109E-4
58-59	0.0	0.0	0.0	1.504769520312383	7.891113159877899E-4
60-61	0.0	3.2879638166157906E-5	0.0	1.760490906149676	8.055511350708688E-4
62-63	0.0	3.2879638166157906E-5	0.0	1.9997560330848072	8.219909541539477E-4
64-65	0.0	3.2879638166157906E-5	0.0	2.233776857732436	8.219909541539477E-4
66-67	0.0	3.2879638166157906E-5	0.0	2.497060560347945	8.219909541539477E-4
68-69	0.0	3.2879638166157906E-5	0.0	2.791596359040388	8.219909541539477E-4
70-71	0.0	3.2879638166157906E-5	0.0	3.1281358954900975	8.548705923201056E-4
72-73	0.0	3.2879638166157906E-5	0.0	3.469541618388398	9.206298686524214E-4
74-75	0.0	3.2879638166157906E-5	0.0	3.8051934046076212	9.535095068185792E-4
76-77	3.2879638166157906E-5	3.2879638166157906E-5	0.0	4.1594057465716405	9.863891449847372E-4
78-79	3.2879638166157906E-5	3.2879638166157906E-5	0.0	4.553764126736539	0.001019268783150895
80-81	3.2879638166157906E-5	3.2879638166157906E-5	0.0	4.98188989529808	0.001068588240400132
82-83	3.2879638166157906E-5	3.2879638166157906E-5	0.0	5.440001893867159	0.0011179076976493688
84-85	3.2879638166157906E-5	3.2879638166157906E-5	0.0	5.947219632037394	0.0011179076976493688
86-87	3.2879638166157906E-5	3.2879638166157906E-5	0.0	6.480987678026801	0.0011343475167324478
88	3.2879638166157906E-5	3.2879638166157906E-5	0.0	6.863854624652626	0.0011836669739816847
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2840	0.0	20.690664	2
GTACCCG	510	0.0	20.282814	1
GTATTAG	915	0.0	17.985506	1
TATACTC	2500	0.0	17.863197	4
GTATAAT	1650	0.0	17.09791	1
TATACTG	1410	0.0	16.002602	5
ACCTGGG	3530	0.0	15.847291	3
GCTTATA	2910	0.0	15.834663	1
GTACTAG	835	0.0	15.766935	1
GTACCTG	3990	0.0	15.673084	1
ATACTCT	2710	0.0	15.437935	5
GTATAGG	1005	0.0	14.971304	1
TAGCCCT	2200	0.0	14.957224	4
ATAGGAC	820	0.0	14.9053545	3
TACGGGC	410	1.22763595E-8	14.905109	4
TGCGCGA	290	2.0308275E-5	14.609982	94
GTACATG	17965	0.0	14.4473295	1
CATGGGG	4735	0.0	14.395397	4
TACATGG	17445	0.0	14.389744	2
GTCCTAC	890	0.0	14.264267	1
>>END_MODULE