FastQCFastQC Report
Mon 16 Jan 2017
ERR1146605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041396
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA347281.1418440742343319No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA280320.9216820170737385No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68840.22634342913583105No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG64330.21151471232289382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.1797529818543853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.17574166599811403No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT48510.15949912474403202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT71050.021.20036512-13
GTACTTT82250.018.45650914-15
TACCTGG27300.018.429552
GTACTAG8200.018.3497331
AGTACTT79900.017.058112-13
TGATCGC3401.9981599E-816.587539
GTCGACG1601.811859E-716.15609636-37
CATGGGG44500.015.9549814
GTATATA21750.015.7818251
CTAGTAC9350.015.5894023
GTACCCG5450.015.5299361
ACTTTTT97700.015.36960916-17
TCGACGA1855.655056E-815.24309736-37
CCGTTTA1850.003375480615.2425969
ATGGGCG3756.664777E-815.0396075
ATTATAC13450.015.0323043
GTATAGA11100.014.8265031
GTATAAT14950.014.468061
TACTTTT95050.014.38877514-15
CGACGAG3450.014.30397424-25
Produced by FastQC (version 0.11.5)