FastQCFastQC Report
Mon 16 Jan 2017
ERR1146604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052332
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA66380.21747306649473255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG61600.021.2120864
GTACTAG12950.019.6042461
CCGCGTA1200.00507322319.5785189
ATGGGGG42300.018.7783995
GCATCGT11650.018.1503418
CAATGCG6100.017.33109318-19
ACATGGG171500.017.102283
CTAGGCA20550.016.925414
TACATGG178300.016.9245552
GTACATG186600.016.8806571
CTAACGC5850.016.873183
CTAGTAC16450.016.8585283
TAACGCC6500.016.631594
TAGGCAT19250.016.6031465
CTGTGCG17250.016.343819
GTATAGA11200.015.5313731
CGACGAG6700.015.43005524-25
GGCGTCG7700.015.256248
GTACCCG4351.6407284E-915.1309061
TACTATA28100.015.0546192
Produced by FastQC (version 0.11.5)