##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146604_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3052332 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.83970256184452 31.0 31.0 34.0 27.0 34.0 2 30.996586216702507 31.0 31.0 34.0 27.0 34.0 3 31.013705586417206 31.0 31.0 34.0 27.0 34.0 4 34.50170066689993 37.0 35.0 37.0 30.0 37.0 5 34.54036618559187 37.0 35.0 37.0 30.0 37.0 6 34.65482195252679 37.0 35.0 37.0 31.0 37.0 7 34.51074850311172 37.0 35.0 37.0 30.0 37.0 8 34.53961954335243 37.0 35.0 37.0 30.0 37.0 9 36.06482322368602 39.0 37.0 39.0 30.0 39.0 10-11 36.07661879507209 39.0 37.0 39.0 30.0 39.0 12-13 36.073414359905804 39.0 37.0 39.0 30.0 39.0 14-15 37.32767864046244 40.0 38.0 41.0 31.0 41.0 16-17 37.31971309149856 40.0 38.0 41.0 31.0 41.0 18-19 37.312954652377265 40.0 37.5 41.0 30.5 41.0 20-21 37.406960317553924 40.0 38.0 41.0 31.0 41.0 22-23 37.41209213152436 40.0 38.0 41.0 30.0 41.0 24-25 37.41544727113565 40.0 37.5 41.0 30.0 41.0 26-27 37.44687766599439 40.0 37.0 41.0 30.0 41.0 28-29 37.41354593799102 40.0 37.0 41.0 30.0 41.0 30-31 37.39211019640065 40.0 37.0 41.0 30.0 41.0 32-33 37.357532699588376 40.0 37.0 41.0 30.0 41.0 34-35 37.3206961103838 40.0 37.0 41.0 30.0 41.0 36-37 37.24987779835221 40.0 37.0 41.0 30.0 41.0 38-39 37.17992046736724 40.0 37.0 41.0 30.0 41.0 40-41 37.115392591631576 40.0 37.0 41.0 30.0 41.0 42-43 36.97133306599675 40.0 36.5 41.0 30.0 41.0 44-45 36.86017756259804 40.0 36.0 41.0 29.5 41.0 46-47 36.75269515242772 40.0 36.0 41.0 29.0 41.0 48-49 36.65179443127418 40.0 35.5 41.0 29.0 41.0 50-51 36.52151486142398 39.0 35.0 41.0 29.0 41.0 52-53 36.39272349796811 39.0 35.0 41.0 28.0 41.0 54-55 36.28557918994395 39.0 35.0 41.0 28.0 41.0 56-57 36.1063914410359 39.0 35.0 41.0 28.0 41.0 58-59 35.93719277588414 39.0 35.0 40.0 28.0 41.0 60-61 35.729283380706946 38.0 34.5 40.0 28.0 41.0 62-63 35.48556791987241 38.0 34.0 40.0 27.5 41.0 64-65 35.19563009528452 37.0 34.0 40.0 27.0 41.0 66-67 34.964395747251615 37.0 34.0 40.0 26.5 41.0 68-69 34.73168465946693 36.0 34.0 39.5 26.0 41.0 70-71 34.434451756886205 36.0 33.5 39.0 26.0 41.0 72-73 34.094314281670535 35.5 33.0 39.0 26.0 40.5 74-75 33.76109872713715 35.0 33.0 38.0 26.0 40.0 76-77 33.4671220234234 35.0 33.0 37.0 26.0 39.0 78-79 33.155759104841806 35.0 33.0 37.0 26.0 39.0 80-81 32.92225387015567 35.0 32.5 36.0 25.5 39.0 82-83 32.662017434538576 35.0 32.0 36.0 25.0 37.5 84-85 32.459198737227794 35.0 32.0 35.5 25.0 37.0 86-87 32.23531417945361 35.0 32.0 35.0 25.0 37.0 88-89 32.04115787535563 35.0 32.0 35.0 24.5 36.0 90-91 31.89088834373194 35.0 32.0 35.0 24.0 36.0 92-93 31.735481756244077 35.0 32.0 35.0 24.0 36.0 94-95 31.624782461409836 35.0 32.0 35.0 24.0 35.5 96-97 31.543731808990636 35.0 31.5 35.0 23.5 35.0 98-99 31.4325669684687 35.0 31.0 35.0 23.0 35.0 100 30.202622781532284 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3185658519555439 1101 2 0.3714574813017215 1101 3 0.3766135461975999 1101 4 0.3601615157170599 1101 5 0.30142683230238987 1101 6 0.36870776907494474 1101 7 0.3191803855179245 1101 8 0.3808125145491985 1101 9 0.5811225597037719 1101 10-11 0.6279517948518176 1101 12-13 0.677180942748798 1101 14-15 0.7450115930896146 1101 16-17 0.7492431978757708 1101 18-19 0.7411893615689706 1101 20-21 0.7261232856358291 1101 22-23 0.7629978819604517 1101 24-25 0.7351344758008622 1101 26-27 0.7301770100200144 1101 28-29 0.7433898379678894 1101 30-31 0.7793362018313417 1101 32-33 0.8301199428172126 1101 34-35 0.7147241118621395 1101 36-37 0.748648823933415 1101 38-39 0.780149719435002 1101 40-41 0.8367622198255162 1101 42-43 0.760552650722353 1101 44-45 0.6886822861416135 1101 46-47 0.6926286347416806 1101 48-49 0.6948840174769018 1101 50-51 0.6286573303048257 1101 52-53 0.6452946461482014 1101 54-55 0.6103957429118978 1101 56-57 0.733056846316309 1101 58-59 0.7126657175367441 1101 60-61 0.7857444272057066 1101 62-63 0.7658076902724318 1101 64-65 0.759383982412551 1101 66-67 0.6496055287372045 1101 68-69 0.7158967423243041 1101 70-71 0.6182584536014417 1101 72-73 0.6501080759104809 1101 74-75 0.59948444684251 1101 76-77 0.5614653526240119 1101 78-79 0.5868732679687625 1101 80-81 0.611070791833825 1101 82-83 0.5599229358743258 1101 84-85 0.5193160989502132 1101 86-87 0.49772427655294393 1101 88-89 0.531022673879896 1101 90-91 0.5315731064261584 1101 92-93 0.46933644461405777 1101 94-95 0.45761809110582163 1101 96-97 0.4841568843787023 1101 98-99 0.5417343513874187 1101 100 0.42999857710733735 1102 1 0.20984530231522314 1102 2 0.30663527548818337 1102 3 0.2707596757498614 1102 4 0.35340565975231186 1102 5 0.27673269000241874 1102 6 0.38060962175343604 1102 7 0.30472222905329716 1102 8 0.32762038098305624 1102 9 0.47027288384172294 1102 10-11 0.5168442507009559 1102 12-13 0.5019797103424253 1102 14-15 0.5778484673872768 1102 16-17 0.589179902116193 1102 18-19 0.4811771966720926 1102 20-21 0.5150518232991388 1102 22-23 0.5051349186561893 1102 24-25 0.5809291792538289 1102 26-27 0.48013006775929057 1102 28-29 0.56126065789271 1102 30-31 0.49421426564410353 1102 32-33 0.5596383373619744 1102 34-35 0.5053876283012073 1102 36-37 0.5288174690164098 1102 38-39 0.4539337084441115 1102 40-41 0.5151092718276047 1102 42-43 0.5523944508159389 1102 44-45 0.4607198076381138 1102 46-47 0.48147223354062163 1102 48-49 0.5259333161254176 1102 50-51 0.4973770003387372 1102 52-53 0.5211957640262241 1102 54-55 0.45847939767056545 1102 56-57 0.5227266767736722 1102 58-59 0.4670559408610302 1102 60-61 0.5003811078702753 1102 62-63 0.4692908419201629 1102 64-65 0.5656599442651569 1102 66-67 0.49276210107256446 1102 68-69 0.42738368405845506 1102 70-71 0.4247755891721283 1102 72-73 0.42068655774571084 1102 74-75 0.4694587527612484 1102 76-77 0.48024779185038824 1102 78-79 0.4582740621276926 1102 80-81 0.4333242058436184 1102 82-83 0.43380754108662245 1102 84-85 0.459526588166959 1102 86-87 0.4565658767903784 1102 88-89 0.3612088512540268 1102 90-91 0.4474200836317834 1102 92-93 0.4738864331851502 1102 94-95 0.4249638099357398 1102 96-97 0.42612497082187417 1102 98-99 0.46896669672927516 1102 100 0.383444839139095 1103 1 0.16143499024130037 1103 2 0.2539676416652945 1103 3 0.21983975457720462 1103 4 0.19491696627662947 1103 5 0.22972092757368756 1103 6 0.3214084649612161 1103 7 0.2989382679697883 1103 8 0.25399822279513984 1103 9 0.4518621258259188 1103 10-11 0.3645973203466397 1103 12-13 0.42047766422823685 1103 14-15 0.6239583835217459 1103 16-17 0.5582576278885014 1103 18-19 0.5130136517567365 1103 20-21 0.4833164167472077 1103 22-23 0.45248863311486076 1103 24-25 0.5044629555560931 1103 26-27 0.46892032231620107 1103 28-29 0.46182178561331 1103 30-31 0.48193900374812415 1103 32-33 0.48540704154025605 1103 34-35 0.462356608773689 1103 36-37 0.500855015425202 1103 38-39 0.48807530353078477 1103 40-41 0.41552570563987246 1103 42-43 0.49984779058854656 1103 44-45 0.4391068073885762 1103 46-47 0.5153984481866871 1103 48-49 0.38899215483583305 1103 50-51 0.44834379284956327 1103 52-53 0.4125054953320131 1103 54-55 0.41698687763400955 1103 56-57 0.48676763131700795 1103 58-59 0.5188473463275187 1103 60-61 0.4084691652620549 1103 62-63 0.44374026973877534 1103 64-65 0.3438536709065829 1103 66-67 0.4070553923039242 1103 68-69 0.4223715819548488 1103 70-71 0.37154483512236425 1103 72-73 0.3453294958764914 1103 74-75 0.3861629765899153 1103 76-77 0.3445780393291642 1103 78-79 0.40937795804675403 1103 80-81 0.25073804655127674 1103 82-83 0.32168351990533495 1103 84-85 0.3144550524474852 1103 86-87 0.2954214570888638 1103 88-89 0.30506714041222693 1103 90-91 0.3849905922617083 1103 92-93 0.27694806140435446 1103 94-95 0.3527112626113471 1103 96-97 0.3819634411842934 1103 98-99 0.3450408710963444 1103 100 0.3008450169465604 1104 1 0.23098167797265745 1104 2 0.19056717966904202 1104 3 0.19740666733873624 1104 4 0.21960066300432146 1104 5 0.15528140059324613 1104 6 0.18419180005165003 1104 7 0.2597346965796845 1104 8 0.3455465310205099 1104 9 0.36916021230911156 1104 10-11 0.40305924857958786 1104 12-13 0.374472045079564 1104 14-15 0.5027133632034904 1104 16-17 0.48308884676772834 1104 18-19 0.49385036297628915 1104 20-21 0.5847575538753702 1104 22-23 0.5336980547738008 1104 24-25 0.4808135162373688 1104 26-27 0.5009041742541314 1104 28-29 0.588909063026847 1104 30-31 0.6035927289769205 1104 32-33 0.5337011269811853 1104 34-35 0.519808140791028 1104 36-37 0.47252812535334954 1104 38-39 0.5158126674101098 1104 40-41 0.5022004876873467 1104 42-43 0.5448293659716796 1104 44-45 0.551290522023308 1104 46-47 0.45042030989353066 1104 48-49 0.44573684967046745 1104 50-51 0.44282845193061604 1104 52-53 0.4185297940407082 1104 54-55 0.44277606198518527 1104 56-57 0.4819839586722878 1104 58-59 0.4725816374123255 1104 60-61 0.3837859991132362 1104 62-63 0.44321746384029126 1104 64-65 0.45489629607504156 1104 66-67 0.44821531413692384 1104 68-69 0.39702208832075314 1104 70-71 0.4661645848918141 1104 72-73 0.4016538395941893 1104 74-75 0.3586927591761935 1104 76-77 0.3788720317065497 1104 78-79 0.40740293122731686 1104 80-81 0.29702015035383766 1104 82-83 0.2923472831028846 1104 84-85 0.34733834170121725 1104 86-87 0.25131109222515136 1104 88-89 0.2771141227475056 1104 90-91 0.29888538664090447 1104 92-93 0.2729207289517461 1104 94-95 0.25949099422226496 1104 96-97 0.34530314659310335 1104 98-99 0.3388559396320119 1104 100 0.3624602401884225 1105 1 0.1560197883039507 1105 2 0.17921927879493538 1105 3 0.2406394227065789 1105 4 0.17004541798702633 1105 5 0.10623073688276463 1105 6 0.17485806076327037 1105 7 0.12199503347032703 1105 8 0.2757677504990639 1105 9 0.3551871354560916 1105 10-11 0.4140694521035897 1105 12-13 0.4393287507543491 1105 14-15 0.487259903607729 1105 16-17 0.4250174715429651 1105 18-19 0.4273393644569836 1105 20-21 0.4636381588524827 1105 22-23 0.3642951730134101 1105 24-25 0.4274910864402628 1105 26-27 0.4202783789335456 1105 28-29 0.4521644581954618 1105 30-31 0.4974540901233837 1105 32-33 0.45773676254805196 1105 34-35 0.496641457209563 1105 36-37 0.5085616062357232 1105 38-39 0.5199056831616815 1105 40-41 0.4760849830670111 1105 42-43 0.44348570680737964 1105 44-45 0.43242831513716595 1105 46-47 0.4684456508567365 1105 48-49 0.5198193262044413 1105 50-51 0.42765952518728767 1105 52-53 0.4547285975142188 1105 54-55 0.3786912434028551 1105 56-57 0.4575136129078885 1105 58-59 0.47486724240244627 1105 60-61 0.4666896706643726 1105 62-63 0.4394948040019955 1105 64-65 0.5042635526776706 1105 66-67 0.39493995951176686 1105 68-69 0.38292498699593835 1105 70-71 0.29571210114953317 1105 72-73 0.35377872802160937 1105 74-75 0.46277388252667606 1105 76-77 0.4494792727996071 1105 78-79 0.4145533349704351 1105 80-81 0.40209747698371245 1105 82-83 0.4056452254832905 1105 84-85 0.34908014908594964 1105 86-87 0.32605847628725115 1105 88-89 0.28769107334850474 1105 90-91 0.28672068339144374 1105 92-93 0.3683565451165691 1105 94-95 0.3614781962291431 1105 96-97 0.34037100093851436 1105 98-99 0.3070531869445148 1105 100 0.3883114056177064 1106 1 0.07144519622935874 1106 2 0.1271140420776291 1106 3 0.1122350540866961 1106 4 0.0836052202537303 1106 5 0.053438859220193535 1106 6 0.1909195710920244 1106 7 0.11738798821673413 1106 8 0.24710134506541692 1106 9 0.27364395162842925 1106 10-11 0.297413284994839 1106 12-13 0.284097408820422 1106 14-15 0.285577512722746 1106 16-17 0.32851719021508785 1106 18-19 0.380909816992947 1106 20-21 0.33948445336257294 1106 22-23 0.33125964532271013 1106 24-25 0.32145012309326404 1106 26-27 0.3185994544097923 1106 28-29 0.3040660336315213 1106 30-31 0.31019723778032215 1106 32-33 0.36318869567239176 1106 34-35 0.34942449313915347 1106 36-37 0.37581634861978586 1106 38-39 0.3125492608483711 1106 40-41 0.33185778067170446 1106 42-43 0.4201599090419279 1106 44-45 0.34014523901787896 1106 46-47 0.3967276920913676 1106 48-49 0.33900332467723615 1106 50-51 0.30733107490194556 1106 52-53 0.3518095194658315 1106 54-55 0.30265259096334063 1106 56-57 0.28991082776200017 1106 58-59 0.22031530726861348 1106 60-61 0.2697164068592173 1106 62-63 0.3745473017915586 1106 64-65 0.3195833621785127 1106 66-67 0.35452369474204914 1106 68-69 0.3004385510181251 1106 70-71 0.328703269463972 1106 72-73 0.4325183791750504 1106 74-75 0.4104938206345423 1106 76-77 0.3529809507193846 1106 78-79 0.3479376589797525 1106 80-81 0.3317283848689243 1106 82-83 0.31116538061551324 1106 84-85 0.37148208622926404 1106 86-87 0.2907184212273677 1106 88-89 0.30383351567629546 1106 90-91 0.28419229941909663 1106 92-93 0.375104796864818 1106 94-95 0.29534826294403516 1106 96-97 0.3001774373182293 1106 98-99 0.31045459157436284 1106 100 0.33683674522063 1107 1 -0.021208512206623453 1107 2 0.05027388278396927 1107 3 0.04195887239092855 1107 4 0.004745485896179957 1107 5 0.00500434788907711 1107 6 -0.004226926283685373 1107 7 -0.01086645700981137 1107 8 -0.07066148564872776 1107 9 0.08813464419625205 1107 10-11 0.1283545196354723 1107 12-13 0.21468602835187056 1107 14-15 0.23653403022056096 1107 16-17 0.20337985490269972 1107 18-19 0.21184048370042063 1107 20-21 0.2836145164067503 1107 22-23 0.2767092176322379 1107 24-25 0.3032007370853975 1107 26-27 0.3001546333665033 1107 28-29 0.301317605910441 1107 30-31 0.28182869713059944 1107 32-33 0.35652219477722724 1107 34-35 0.27222358955695825 1107 36-37 0.2633162529823778 1107 38-39 0.19955280708331458 1107 40-41 0.27154362136273846 1107 42-43 0.2839530223837272 1107 44-45 0.1660370578117778 1107 46-47 0.179981152252644 1107 48-49 0.25695833875506935 1107 50-51 0.19424319177849725 1107 52-53 0.15588204704765474 1107 54-55 0.13879210107030104 1107 56-57 0.19987232414307243 1107 58-59 0.08193960337732165 1107 60-61 0.23286487627819952 1107 62-63 0.2769011774017329 1107 64-65 0.23187994141984092 1107 66-67 0.22181312030554778 1107 68-69 0.21395871520842036 1107 70-71 0.19362351743264128 1107 72-73 0.2749148229938143 1107 74-75 0.30166218454634475 1107 76-77 0.2766245732347272 1107 78-79 0.24653728828917565 1107 80-81 0.20222046800461158 1107 82-83 0.22515225533815908 1107 84-85 0.19566859446938167 1107 86-87 0.19141977813086442 1107 88-89 0.15670689366591972 1107 90-91 0.15095997512413106 1107 92-93 0.16553378800496787 1107 94-95 0.17076313800333054 1107 96-97 0.22963179723620897 1107 98-99 0.27031131717917845 1107 100 0.3395259733885254 1108 1 -0.06671434960274425 1108 2 -0.05467540629952694 1108 3 -0.07652787549907103 1108 4 -0.1362698258308228 1108 5 -0.05402036983150538 1108 6 -0.05085947217045117 1108 7 -0.04415997710399466 1108 8 -0.1404033466751926 1108 9 -0.12238619729315303 1108 10-11 -0.08175998126357342 1108 12-13 -0.150500259153155 1108 14-15 -0.11932328796397229 1108 16-17 -0.17105897996238895 1108 18-19 -0.06671925306889648 1108 20-21 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-1.250066267549343 2215 18-19 -1.2441807406143326 2215 20-21 -1.1292385215923204 2215 22-23 -1.104792934352183 2215 24-25 -1.0400966790694923 2215 26-27 -1.0465649305633988 2215 28-29 -1.058372930857253 2215 30-31 -1.0381653131563553 2215 32-33 -0.973129535454639 2215 34-35 -0.985362358275566 2215 36-37 -0.9968470517567383 2215 38-39 -1.0261606472601557 2215 40-41 -1.103307614974952 2215 42-43 -1.2118980133737551 2215 44-45 -1.0816579115018214 2215 46-47 -1.1277928543708526 2215 48-49 -1.124052352131038 2215 50-51 -0.967902375655818 2215 52-53 -0.9825765394670967 2215 54-55 -0.9961472323149891 2215 56-57 -1.0628721750334336 2215 58-59 -1.0711779947873126 2215 60-61 -1.0209339429491777 2215 62-63 -1.042244026456217 2215 64-65 -1.0207883491421725 2215 66-67 -0.998640424405842 2215 68-69 -1.0393200394693167 2215 70-71 -0.9301614392092006 2215 72-73 -0.8529760938081239 2215 74-75 -0.8069818931743313 2215 76-77 -0.8986927648481782 2215 78-79 -0.9155708137669833 2215 80-81 -0.8530369689916455 2215 82-83 -0.891649562306327 2215 84-85 -0.8101272441438496 2215 86-87 -0.8372261036249284 2215 88-89 -0.8450071770562175 2215 90-91 -0.7983577356344469 2215 92-93 -0.8569589493690621 2215 94-95 -0.8949702438068527 2215 96-97 -0.879314990453782 2215 98-99 -1.0365803145112338 2215 100 -0.9350168362458149 2216 1 -0.3842373385825759 2216 2 -0.4482170980417983 2216 3 -0.4091840836509171 2216 4 -0.3701029985816575 2216 5 -0.4156791466327192 2216 6 -0.46810101866850573 2216 7 -0.48092433971060444 2216 8 -0.4081895106691249 2216 9 -0.5264296413986287 2216 10-11 -0.633104722128472 2216 12-13 -0.5913259526373906 2216 14-15 -0.7511961785803649 2216 16-17 -0.7333361325381773 2216 18-19 -0.7577877533703514 2216 20-21 -0.8017937590622282 2216 22-23 -0.8435523369934614 2216 24-25 -0.8003075938730149 2216 26-27 -0.9498115062524306 2216 28-29 -0.939421671601572 2216 30-31 -0.9560102481379005 2216 32-33 -0.8955674657179173 2216 34-35 -0.909962180602605 2216 36-37 -0.855373617320744 2216 38-39 -0.8423792895001156 2216 40-41 -0.8941733645618584 2216 42-43 -0.9312720400877623 2216 44-45 -0.7738401967779254 2216 46-47 -0.7323306127722091 2216 48-49 -0.7402166535697745 2216 50-51 -0.7874147152218569 2216 52-53 -0.8484779713645594 2216 54-55 -0.8356348104511611 2216 56-57 -0.7395753663587712 2216 58-59 -0.8126739255267239 2216 60-61 -0.7735420133818067 2216 62-63 -0.7469499445572083 2216 64-65 -0.826821687262445 2216 66-67 -0.8438809318871421 2216 68-69 -0.9255454228522382 2216 70-71 -0.8699569620271035 2216 72-73 -0.8263206018551017 2216 74-75 -0.768230595670218 2216 76-77 -0.9424237360090189 2216 78-79 -0.7905426218024374 2216 80-81 -0.7673715643480534 2216 82-83 -0.7981052804822859 2216 84-85 -0.7630307714180233 2216 86-87 -0.8300291730127469 2216 88-89 -0.8457810391139908 2216 90-91 -0.776315367057343 2216 92-93 -0.7703689998806418 2216 94-95 -0.8584300553465809 2216 96-97 -0.858234525811806 2216 98-99 -0.8446997990554976 2216 100 -0.8447734102750744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 913.0 1 210.0 2 164.0 3 137.0 4 145.0 5 175.0 6 306.0 7 861.0 8 2150.0 9 2860.0 10 3342.0 11 4865.0 12 7256.0 13 9549.0 14 11162.0 15 11842.0 16 12500.0 17 13529.0 18 14394.0 19 15714.0 20 16693.0 21 18255.0 22 20163.0 23 23285.0 24 27430.0 25 32490.0 26 34320.0 27 33527.0 28 36186.0 29 42805.0 30 50133.0 31 58779.0 32 73982.0 33 97900.0 34 141178.0 35 214206.0 36 373520.0 37 682249.0 38 751548.0 39 211288.0 40 321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44407542873482 20.11103918802553 13.378284168766974 24.066601214472673 2 14.252928424592309 20.965628606954002 40.807026711087346 23.97441625736634 3 17.878910279066076 26.929656778691335 30.677990440975417 24.51344250126717 4 12.117711937092302 17.92763804992433 39.66445206251951 30.29019795046386 5 12.770508551486625 39.18382188042214 35.48519407894305 12.560475489148182 6 29.521958020495948 38.296247525226455 19.08426657188369 13.097527882393909 7 25.733614919752135 33.71892442381838 23.21904295373268 17.32841770269681 8 24.88190298256653 35.81020931378641 21.380486670865817 17.927401032781244 9 26.20534234597816 16.33491605395391 20.74775926953777 36.711982330530155 10-11 23.743426912148287 26.882804325135655 29.124371338459948 20.24939742425611 12-13 24.977342663670058 24.637052338323983 28.347823264744726 22.037781733261234 14-15 22.804612383194154 25.796848522840364 26.03697230920954 25.36156678475594 16-17 21.942736581883278 28.66629891041966 27.259508315908626 22.131456191788434 18-19 21.827013901798566 28.905714914278093 28.04482371853194 21.222447465391404 20-21 22.833698681647995 28.250453932918738 27.057024662117694 21.858822723315573 22-23 22.59897461654672 27.905766749197426 26.323399043202755 23.171859591053106 24-25 22.44837539539739 28.157916996710924 26.863170864130176 22.53053674376151 26-27 22.800017572255246 27.798570156081624 26.85104530330663 22.5503669683565 28-29 22.417417474031513 27.885886351598337 27.31180334915358 22.38489282521657 30-31 22.64633487777024 28.13035371004216 27.098851066159845 22.12446034602775 32-33 22.36134452541605 28.40344287534291 26.738336129386692 22.49687646985435 34-35 22.518084581229417 28.29316605058125 26.993033728975647 22.195715639213688 36-37 22.956295317699762 27.76085272118513 26.958303371556525 22.324548589558578 38-39 23.134261921192177 27.500844759515285 27.122395775694173 22.242497543598365 40-41 22.7005018386542 27.452951601506335 27.633406945079734 22.21313961475973 42-43 22.69268996173598 27.643157252374984 27.171062706236 22.493090079653044 44-45 22.566903573273002 28.185229815803066 27.015911270688342 22.231955340235597 46-47 22.350367237195883 27.914617351957503 26.8966981441406 22.838317266706014 48-49 22.699922234542782 27.574371928752218 27.192447290251742 22.53325854645326 50-51 22.69275616659715 27.648626599196763 26.892852833237583 22.765764400968504 52-53 22.229545859606198 27.897860836570352 26.958555153466 22.914038150357445 54-55 22.32707356705029 27.855263172995375 26.975738220741647 22.841925039212686 56-57 22.61934626197201 27.706066779930804 26.967341299861715 22.707245658235472 58-59 22.26365830231366 27.64176599465667 27.330820496692965 22.763755206336704 60-61 22.527158019971985 27.674363809660292 27.067265053387995 22.731213116979728 62-63 22.73201507767818 27.45711176446186 26.945209210245203 22.86566394761476 64-65 22.089267762458757 27.4965184992186 27.294425023976565 23.11978871434608 66-67 22.59863095146348 27.234394712703462 27.154703534057813 23.012270801775244 68-69 22.55022959641697 27.38519154350608 27.04389181708696 23.020687042989994 70-71 22.160619761709324 27.264822331413 27.173473527211645 23.401084379666028 72-73 22.432340920922645 27.144024824657407 27.18665633672336 23.236977917696585 74-75 22.456083679181702 27.415377148263598 27.082608431868003 23.04593074068669 76-77 22.382275229008798 27.157050860564112 27.207045334772772 23.253628575654314 78-79 22.22897823220144 27.198138358492912 27.33453760851021 23.238345800795436 80-81 22.02786074383316 27.450826385637882 27.54001159292913 22.981301277599822 82-83 22.21870394214264 27.0382210934574 27.565793659487188 23.17728130491277 84-85 22.3628830358074 27.149358958802438 27.063108467699355 23.424649537690808 86-87 22.065622697772465 27.26106311282397 27.49357118460895 23.179743004794616 88-89 22.565067117951067 26.56043271881852 27.546587074283035 23.327913088947376 90-91 22.36031087737973 26.617076087368226 27.698611139122363 23.324001896129676 92-93 22.24540971301545 26.949500858654936 27.502060148802915 23.303029279526697 94-95 21.76731137980307 26.94660437327177 27.777711283395323 23.508372963529837 96-97 22.067272812231778 26.64484411467415 27.438637393951172 23.849245679142903 98-99 22.020710350255804 26.818263806900173 27.465171192443922 23.695854650400104 100 22.173588896406308 26.97751561689186 27.032692017157235 23.816203469544597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2041.0 1 1365.5 2 661.0 3 609.0 4 554.5 5 470.0 6 394.5 7 394.0 8 448.0 9 476.5 10 417.0 11 376.0 12 383.0 13 414.5 14 453.5 15 518.0 16 593.0 17 702.0 18 925.0 19 1268.5 20 1826.5 21 2603.0 22 3801.0 23 5461.5 24 7794.0 25 11019.0 26 15041.5 27 19613.5 28 24197.0 29 29585.0 30 36273.5 31 42971.5 32 50535.0 33 57608.0 34 63504.0 35 69364.0 36 76352.0 37 85663.0 38 91113.5 39 91348.5 40 92508.0 41 93259.0 42 93987.5 43 99352.0 44 108938.0 45 117450.0 46 119964.5 47 123120.5 48 140231.0 49 143228.5 50 135899.5 51 130357.0 52 114386.5 53 105109.5 54 98329.5 55 88317.0 56 79474.0 57 69861.5 58 60039.5 59 53007.0 60 44653.5 61 35081.5 62 27064.5 63 20928.5 64 15352.0 65 10686.0 66 7852.0 67 5879.5 68 4456.0 69 3488.0 70 2721.0 71 2086.0 72 1678.5 73 1422.5 74 1101.0 75 829.0 76 644.0 77 432.5 78 268.0 79 182.5 80 123.0 81 81.0 82 58.5 83 51.0 84 39.5 85 35.5 86 30.5 87 21.5 88 22.0 89 17.0 90 12.0 91 12.5 92 11.0 93 12.0 94 13.0 95 9.0 96 5.5 97 8.5 98 6.5 99 3.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09101238004253798 2 0.059004066399067995 3 0.07305889398663054 4 0.07456593843657898 5 0.07653164858868564 6 0.08380477615148024 7 0.08088897275918872 8 0.08219944619392648 9 0.06876709348786436 10-11 0.07536860341535587 12-13 0.06537624347548039 14-15 0.07333736959151232 16-17 0.07549965075882964 18-19 0.07518841331807943 20-21 0.06704709710477104 22-23 0.07063451813236568 24-25 0.07325546500184121 26-27 0.06778423841181104 28-29 0.0765807913424883 30-31 0.08062687807224116 32-33 0.08182268508143936 34-35 0.08405048992049358 36-37 0.08560667712424468 38-39 0.086736960461706 40-41 0.08344439595692736 42-43 0.08947257375672109 44-45 0.08541010610903402 46-47 0.0829529684189007 48-49 0.09099599912460374 50-51 0.08767067278395665 52-53 0.09322380396365795 54-55 0.08575410538565267 56-57 0.09248666265661795 58-59 0.09576284624346237 60-61 0.09535332329510683 62-63 0.0957792271613966 64-65 0.0983018885232668 66-67 0.10028397959330768 68-69 0.09695865325266059 70-71 0.09930112451725435 72-73 0.09646722571463393 74-75 0.10048055060851835 76-77 0.09812169842599036 78-79 0.1046740655996792 80-81 0.100234836839505 82-83 0.10411711438991565 84-85 0.10070988345959744 86-87 0.0974828426265557 88-89 0.09702417692439748 90-91 0.09705693876026592 92-93 0.09156933125230152 94-95 0.09122533197568286 96-97 0.08958724018226065 98-99 0.10261006993996721 100 0.24741738447849054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3052332.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.135001288324474 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90292179535395 46.65861062760615 2 14.458583187629543 18.25685336356676 3 5.348035752662215 10.129447324030028 4 2.5312334006068595 6.392376960334562 5 1.3614564812051635 4.297777834744285 6 0.755545226343539 2.862080930315205 7 0.49245312590957174 2.1763720117118157 8 0.3101962355375305 1.5667391784236313 9 0.20454769798317948 1.1622707258122658 >10 0.6291555112064887 5.672307911765177 >50 0.004091139330587598 0.171887159724518 >100 0.0015484764372980834 0.16529080240581018 >500 0.0 0.0 >1k 1.546465294289267E-4 0.16393099233512 >5k 7.732326471446335E-5 0.32405417722469526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6638 0.21747306649473255 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.552367173688838E-5 0.0 16-17 0.0 0.0 0.0 6.552367173688838E-5 0.0 18-19 0.0 0.0 0.0 1.4742826140799886E-4 0.0 20-21 0.0 0.0 0.0 1.9657101521066515E-4 0.0 22-23 0.0 0.0 0.0 3.43999276618664E-4 0.0 24-25 0.0 0.0 0.0 7.535222249742164E-4 3.276183586844419E-5 26-27 0.0 0.0 0.0 0.0012940925168035454 3.276183586844419E-5 28-29 0.0 0.0 0.0 0.004242657744963523 3.276183586844419E-5 30-31 0.0 0.0 0.0 0.012809877824561678 3.276183586844419E-5 32-33 0.0 0.0 0.0 0.03269631219670731 3.276183586844419E-5 34-35 0.0 0.0 0.0 0.06601509927491506 3.276183586844419E-5 36-37 0.0 0.0 0.0 0.12010489029371642 3.276183586844419E-5 38-39 0.0 0.0 0.0 0.19765215579432382 3.276183586844419E-5 40-41 0.0 0.0 0.0 0.2859289225418467 3.276183586844419E-5 42-43 0.0 0.0 0.0 0.3708967438666567 3.276183586844419E-5 44-45 0.0 0.0 0.0 0.47655366454238923 3.276183586844419E-5 46-47 0.0 0.0 0.0 0.608993386040575 3.276183586844419E-5 48-49 0.0 0.0 0.0 0.754226604445388 3.276183586844419E-5 50-51 0.0 0.0 0.0 0.9067657122488642 3.276183586844419E-5 52-53 0.0 0.0 0.0 1.0650053794934495 3.276183586844419E-5 54-55 0.0 0.0 0.0 1.2324675035350021 3.276183586844419E-5 56-57 0.0 0.0 0.0 1.4311680380771161 3.276183586844419E-5 58-59 0.0 0.0 0.0 1.6604189845665545 3.276183586844419E-5 60-61 0.0 0.0 0.0 1.9077380835374396 3.276183586844419E-5 62-63 1.6380917934222095E-5 0.0 0.0 2.1599878388065257 3.276183586844419E-5 64-65 3.276183586844419E-5 0.0 0.0 2.4292901296451372 3.276183586844419E-5 66-67 3.276183586844419E-5 0.0 0.0 2.7285695003033745 3.276183586844419E-5 68-69 3.276183586844419E-5 0.0 0.0 3.0527314853036955 3.276183586844419E-5 70-71 3.276183586844419E-5 0.0 0.0 3.4249223216871556 3.276183586844419E-5 72-73 3.276183586844419E-5 0.0 0.0 3.801208387554172 3.276183586844419E-5 74-75 3.276183586844419E-5 0.0 0.0 4.173006081907211 3.276183586844419E-5 76-77 3.276183586844419E-5 0.0 0.0 4.573241049794059 3.276183586844419E-5 78-79 3.276183586844419E-5 0.0 0.0 5.014477455270265 3.276183586844419E-5 80-81 3.276183586844419E-5 0.0 0.0 5.502858142561163 3.276183586844419E-5 82-83 3.276183586844419E-5 0.0 0.0 6.009438029676982 3.276183586844419E-5 84-85 3.276183586844419E-5 0.0 0.0 6.524798088805543 3.276183586844419E-5 86-87 3.276183586844419E-5 0.0 0.0 7.069217896349414 3.276183586844419E-5 88 3.276183586844419E-5 0.0 0.0 7.488733204644842 3.276183586844419E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 6160 0.0 21.212086 4 GTACTAG 1295 0.0 19.604246 1 CCGCGTA 120 0.005073223 19.578518 9 ATGGGGG 4230 0.0 18.778399 5 GCATCGT 1165 0.0 18.150341 8 CAATGCG 610 0.0 17.331093 18-19 ACATGGG 17150 0.0 17.10228 3 CTAGGCA 2055 0.0 16.92541 4 TACATGG 17830 0.0 16.924555 2 GTACATG 18660 0.0 16.880657 1 CTAACGC 585 0.0 16.87318 3 CTAGTAC 1645 0.0 16.858528 3 TAACGCC 650 0.0 16.63159 4 TAGGCAT 1925 0.0 16.603146 5 CTGTGCG 1725 0.0 16.34381 9 GTATAGA 1120 0.0 15.531373 1 CGACGAG 670 0.0 15.430055 24-25 GGCGTCG 770 0.0 15.25624 8 GTACCCG 435 1.6407284E-9 15.130906 1 TACTATA 2810 0.0 15.054619 2 >>END_MODULE