FastQCFastQC Report
Mon 16 Jan 2017
ERR1146604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052332
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80250.26291373284426467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.2507263299012034No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG63220.2071203263603042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.13527362030080606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT94950.022.12566212-13
CATGGGG59400.019.235474
GTACTTT109750.018.90644514-15
TGATCGC6300.018.649889
AGTACTT102850.018.62117212-13
GTATAAG14700.017.9138621
ATATCGT2957.167E-817.524856
CGACGAG6100.017.33521724-25
ATGGGGG41250.017.3182225
GTACTAG13350.016.9074651
ACTTTTT124100.016.83385816-17
GTGATCG6800.016.5874238
TATTCGC1700.001920246516.5874239
GGGCTAT19650.016.5032796
TAGGCAT19000.016.0784235
CTAACGC4701.8189894E-1116.006823
TCTAACG5300.015.969332
CTCGTCG8600.015.8480619
GTGTAGG17850.015.8063491
ACATGGG169800.015.784113
Produced by FastQC (version 0.11.5)