##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146604_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3052332 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28003343017732 33.0 31.0 34.0 30.0 34.0 2 32.333648829812745 34.0 31.0 34.0 30.0 34.0 3 32.46493238612314 34.0 31.0 34.0 30.0 34.0 4 35.997426885410896 37.0 35.0 37.0 35.0 37.0 5 36.053255019440876 37.0 35.0 37.0 35.0 37.0 6 36.12836382149779 37.0 36.0 37.0 35.0 37.0 7 36.09096323728873 37.0 36.0 37.0 35.0 37.0 8 36.09314714126773 37.0 36.0 37.0 35.0 37.0 9 37.873924592737616 39.0 38.0 39.0 35.0 39.0 10-11 37.82611671993741 39.0 38.0 39.0 35.0 39.0 12-13 37.80926665251356 39.0 38.0 39.0 35.0 39.0 14-15 39.16220221129288 40.0 39.0 41.0 36.0 41.0 16-17 39.09279167534855 40.0 39.0 41.0 36.0 41.0 18-19 39.199232914374974 40.0 39.0 41.0 36.0 41.0 20-21 39.25849710975084 40.0 39.0 41.0 36.0 41.0 22-23 39.23419372466691 40.0 39.0 41.0 36.0 41.0 24-25 39.23379288360506 41.0 39.0 41.0 36.0 41.0 26-27 39.15109824226198 40.5 39.0 41.0 35.5 41.0 28-29 39.08506872777929 40.0 39.0 41.0 35.0 41.0 30-31 38.96571408352696 40.0 38.5 41.0 35.0 41.0 32-33 38.87486321933525 40.0 38.0 41.0 35.0 41.0 34-35 38.82547720890126 40.0 38.0 41.0 35.0 41.0 36-37 38.76740275959496 40.0 38.0 41.0 35.0 41.0 38-39 38.684201292651 40.0 38.0 41.0 35.0 41.0 40-41 38.583634086986606 40.0 38.0 41.0 35.0 41.0 42-43 38.50436993747731 40.0 38.0 41.0 34.5 41.0 44-45 38.39067621084469 40.0 38.0 41.0 34.0 41.0 46-47 38.18693428499914 40.0 38.0 41.0 33.5 41.0 48-49 37.9250813804003 40.0 38.0 41.0 33.0 41.0 50-51 37.824793469386684 40.0 37.0 41.0 33.0 41.0 52-53 37.72242469036789 40.0 37.0 41.0 33.0 41.0 54-55 37.57732088121476 40.0 37.0 41.0 33.0 41.0 56-57 37.437151168352585 40.0 36.0 41.0 33.0 41.0 58-59 37.27669827528591 39.5 36.0 41.0 32.0 41.0 60-61 37.076421241201814 39.0 35.5 41.0 32.0 41.0 62-63 36.82533076349493 39.0 35.0 41.0 32.0 41.0 64-65 36.55846267706134 38.5 35.0 41.0 31.0 41.0 66-67 36.29032588853375 37.5 35.0 40.0 31.0 41.0 68-69 36.01567817655484 37.0 35.0 40.0 31.0 41.0 70-71 35.69584009865244 37.0 35.0 39.5 31.0 41.0 72-73 35.366630333790695 36.0 35.0 39.0 31.0 41.0 74-75 35.039873447580405 36.0 35.0 39.0 30.0 40.5 76-77 34.71245116848364 35.0 34.5 37.5 30.0 39.5 78-79 34.418804540266265 35.0 34.0 37.0 30.0 39.0 80-81 34.163000781042165 35.0 34.0 37.0 30.0 39.0 82-83 33.90746665172727 35.0 34.0 36.0 30.0 38.0 84-85 33.69360426716359 35.0 34.0 36.0 30.0 37.0 86-87 33.49421573406825 35.0 34.0 35.5 29.0 37.0 88-89 33.28248827453894 35.0 34.0 35.0 29.0 36.5 90-91 33.18538677968189 35.0 34.0 35.0 29.0 36.0 92-93 33.086438663946126 35.0 34.0 35.0 29.0 36.0 94-95 33.013374528065754 35.0 34.0 35.0 29.0 36.0 96-97 32.9558119824449 35.0 34.0 35.0 29.0 35.5 98-99 32.88731648457639 35.0 34.0 35.0 29.0 35.0 100 32.137034241360375 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3390040479529546 1101 2 0.3126250832982578 1101 3 0.3072340297498144 1101 4 0.2125733997646222 1101 5 0.20687791877136164 1101 6 0.2226778041337596 1101 7 0.2748502519404923 1101 8 0.2846057621915534 1101 9 0.33867617667944927 1101 10-11 0.37160938265429166 1101 12-13 0.33931693004165453 1101 14-15 0.5045378527009348 1101 16-17 0.5243803222624024 1101 18-19 0.45345906663264657 1101 20-21 0.4514800729747819 1101 22-23 0.4567148184965788 1101 24-25 0.44203422504675416 1101 26-27 0.4287754080370618 1101 28-29 0.4272238802197137 1101 30-31 0.5162156382377532 1101 32-33 0.46987565059879444 1101 34-35 0.41097463824056035 1101 36-37 0.4255499859721823 1101 38-39 0.4637282329283039 1101 40-41 0.4572057688252755 1101 42-43 0.5162903792960236 1101 44-45 0.5188264277710601 1101 46-47 0.47702949942264894 1101 48-49 0.559362753284482 1101 50-51 0.49290590769535925 1101 52-53 0.5015018592831311 1101 54-55 0.5596168308259024 1101 56-57 0.550815778771863 1101 58-59 0.4987467598852291 1101 60-61 0.5047120500256739 1101 62-63 0.5244809169013109 1101 64-65 0.533341430571248 1101 66-67 0.4509506748262879 1101 68-69 0.49875773770238396 1101 70-71 0.5112018308540911 1101 72-73 0.4938383934649906 1101 74-75 0.42694427332843077 1101 76-77 0.47267050388880705 1101 78-79 0.4630945131470696 1101 80-81 0.40005557286531257 1101 82-83 0.35212449662289913 1101 84-85 0.3732918082518779 1101 86-87 0.3633726382258118 1101 88-89 0.46576630014773457 1101 90-91 0.46126795024963485 1101 92-93 0.4090492731121813 1101 94-95 0.4130775308135668 1101 96-97 0.3667141017190687 1101 98-99 0.43393928381981794 1101 100 0.4910637617953455 1102 1 0.18170738363589578 1102 2 0.1697491173767247 1102 3 0.19354406288764636 1102 4 0.08441265079471805 1102 5 0.1635789638873888 1102 6 0.17249625815667002 1102 7 0.14868740627611743 1102 8 0.1728961065065988 1102 9 0.24592371467904428 1102 10-11 0.2512059544630816 1102 12-13 0.28700108192754925 1102 14-15 0.3911058139647423 1102 16-17 0.45762157616228905 1102 18-19 0.34987730332694866 1102 20-21 0.3691911874899674 1102 22-23 0.3722461247061162 1102 24-25 0.35421261521144487 1102 26-27 0.35791539231184544 1102 28-29 0.3397015759141908 1102 30-31 0.36901808222717136 1102 32-33 0.36680785867418564 1102 34-35 0.38531176205921724 1102 36-37 0.4025988406288903 1102 38-39 0.3633395905020791 1102 40-41 0.41339029948045436 1102 42-43 0.4383434285384311 1102 44-45 0.38796714205933824 1102 46-47 0.4565094785035768 1102 48-49 0.5946693469817106 1102 50-51 0.5455630071961934 1102 52-53 0.4739927371063999 1102 54-55 0.49728663375816495 1102 56-57 0.5691144344699808 1102 58-59 0.5194807286095369 1102 60-61 0.4811491294451571 1102 62-63 0.5318099232093516 1102 64-65 0.5103337091826887 1102 66-67 0.4173284974952338 1102 68-69 0.4021050290531676 1102 70-71 0.4755055475801413 1102 72-73 0.4771109122867685 1102 74-75 0.5130188172794306 1102 76-77 0.4423933440960326 1102 78-79 0.44103422360696953 1102 80-81 0.3900439764215591 1102 82-83 0.4298659665349689 1102 84-85 0.3833462181915479 1102 86-87 0.37565697010191457 1102 88-89 0.4252180600187714 1102 90-91 0.40546413359552247 1102 92-93 0.4120745806453172 1102 94-95 0.4741025894240778 1102 96-97 0.4393058949313797 1102 98-99 0.4609349432314218 1102 100 0.5109635105988986 1103 1 0.17828217046144346 1103 2 0.13687181496455736 1103 3 0.19445682052582214 1103 4 0.22825262787140588 1103 5 0.16272882787511378 1103 6 0.14742958035726872 1103 7 0.14256661675025128 1103 8 0.1654393889614454 1103 9 0.18286943026870262 1103 10-11 0.18454808012571533 1103 12-13 0.23513092502242472 1103 14-15 0.3075680906024161 1103 16-17 0.360259654475378 1103 18-19 0.33704935814174775 1103 20-21 0.3198814011530615 1103 22-23 0.28637622018511877 1103 24-25 0.2541593868294214 1103 26-27 0.2902328004596839 1103 28-29 0.33242845359043827 1103 30-31 0.36702225510460096 1103 32-33 0.3452660193631729 1103 34-35 0.3356566079673229 1103 36-37 0.2753891748860511 1103 38-39 0.31806842462972185 1103 40-41 0.3283876095969731 1103 42-43 0.34903312984275914 1103 44-45 0.3455632876033832 1103 46-47 0.4060687076503413 1103 48-49 0.3832848302736025 1103 50-51 0.4459353212265711 1103 52-53 0.43762617667510995 1103 54-55 0.4204598613052255 1103 56-57 0.36938940033645196 1103 58-59 0.3890156871321935 1103 60-61 0.3813890841298644 1103 62-63 0.3783437687749327 1103 64-65 0.46064012818399647 1103 66-67 0.42797085305513605 1103 68-69 0.3598880906624018 1103 70-71 0.31867775040969093 1103 72-73 0.2977872448227785 1103 74-75 0.31717015667062043 1103 76-77 0.3353108382062828 1103 78-79 0.3328819316080214 1103 80-81 0.3489774732036537 1103 82-83 0.3424011081784428 1103 84-85 0.2786295763047022 1103 86-87 0.2796323996530532 1103 88-89 0.3404644334201379 1103 90-91 0.3313466952754496 1103 92-93 0.3057036841408447 1103 94-95 0.26616273654694567 1103 96-97 0.2602720896903321 1103 98-99 0.2984579163544083 1103 100 0.2674194681452349 1104 1 0.22482237435972507 1104 2 0.17538022190731084 1104 3 0.17977724162081188 1104 4 0.128789356159686 1104 5 0.09156175224709528 1104 6 0.11444789525042154 1104 7 0.14169705102534635 1104 8 0.15380448389606727 1104 9 0.1465300828414584 1104 10-11 0.18167661376114808 1104 12-13 0.20671991166928905 1104 14-15 0.2646881912171537 1104 16-17 0.3385041164654794 1104 18-19 0.29380174106434254 1104 20-21 0.28692794284257417 1104 22-23 0.3195962678533846 1104 24-25 0.2756721054153175 1104 26-27 0.2193639632195854 1104 28-29 0.29739011210361355 1104 30-31 0.2794792337794192 1104 32-33 0.24365950079840815 1104 34-35 0.2863973995453861 1104 36-37 0.2722728463688 1104 38-39 0.24833436296722766 1104 40-41 0.26954428959302845 1104 42-43 0.2689378707719712 1104 44-45 0.25019956012970823 1104 46-47 0.37104320617238074 1104 48-49 0.4183181312610813 1104 50-51 0.3022941154308896 1104 52-53 0.28579808571754484 1104 54-55 0.3118742408099422 1104 56-57 0.2843875600236103 1104 58-59 0.19558387163824165 1104 60-61 0.1175093396205753 1104 62-63 0.20411166630147193 1104 64-65 0.24715179436668677 1104 66-67 0.29301795599099023 1104 68-69 0.14656344042928282 1104 70-71 0.23272312307476284 1104 72-73 0.22010272394691555 1104 74-75 0.19360918607418398 1104 76-77 0.195999766229491 1104 78-79 0.23163633932573902 1104 80-81 0.2242596814772 1104 82-83 0.20737886445480314 1104 84-85 0.13897604219042137 1104 86-87 0.2469009886034641 1104 88-89 0.30870188593266334 1104 90-91 0.2551408977355436 1104 92-93 0.2463650687532848 1104 94-95 0.2579827223815485 1104 96-97 0.23539680092940785 1104 98-99 0.24864650796408228 1104 100 0.30921581934367026 1105 1 0.13080500677003215 1105 2 0.12254017600316303 1105 3 0.12655996979486872 1105 4 0.12031036043001819 1105 5 0.08594624892788971 1105 6 0.15209096187649607 1105 7 0.14454701160086358 1105 8 0.1511060798719086 1105 9 0.18865884866004734 1105 10-11 0.18861365689907217 1105 12-13 0.19461323917352757 1105 14-15 0.31535775681923184 1105 16-17 0.4045741072794442 1105 18-19 0.3188385502576665 1105 20-21 0.2250291401497435 1105 22-23 0.26712462615555665 1105 24-25 0.24786712517960297 1105 26-27 0.2517725777938864 1105 28-29 0.24871206828648695 1105 30-31 0.3188272595627666 1105 32-33 0.3000743452213328 1105 34-35 0.2652520598312691 1105 36-37 0.22845127402163 1105 38-39 0.27382815784022796 1105 40-41 0.33105027873112647 1105 42-43 0.3390004815313432 1105 44-45 0.3176492776526487 1105 46-47 0.31974191206055735 1105 48-49 0.4267378471445866 1105 50-51 0.3410082007699273 1105 52-53 0.3743811985079901 1105 54-55 0.370446554155194 1105 56-57 0.35200072649900704 1105 58-59 0.317116482059312 1105 60-61 0.28020560977844866 1105 62-63 0.3246856763306454 1105 64-65 0.31538469285970905 1105 66-67 0.2921038545178476 1105 68-69 0.21623420047603048 1105 70-71 0.2939223299010507 1105 72-73 0.2974784327418334 1105 74-75 0.33641259193294815 1105 76-77 0.29605264447680213 1105 78-79 0.3114217816736158 1105 80-81 0.33243124432606663 1105 82-83 0.24541162190447352 1105 84-85 0.26526402566076257 1105 86-87 0.28085892021044856 1105 88-89 0.3596460436930471 1105 90-91 0.2557307816480119 1105 92-93 0.26791948624681794 1105 94-95 0.20540352861868882 1105 96-97 0.18122602917242858 1105 98-99 0.22983889682738123 1105 100 0.2763436093087748 1106 1 0.1118158780826306 1106 2 0.11148037373646247 1106 3 0.13593632619708984 1106 4 0.10133429339360589 1106 5 0.0980980052175795 1106 6 0.07967586260794235 1106 7 0.11352549001900059 1106 8 0.09917928540200904 1106 9 0.06493838808613361 1106 10-11 0.1387400534909915 1106 12-13 0.10400059750485013 1106 14-15 0.22000142493272534 1106 16-17 0.24720587924311133 1106 18-19 0.20646348997311748 1106 20-21 0.16209202697556435 1106 22-23 0.17624944530193432 1106 24-25 0.2004868904717867 1106 26-27 0.1780568897463084 1106 28-29 0.2056792031631005 1106 30-31 0.26813333423522323 1106 32-33 0.21474378497698865 1106 34-35 0.23768665110206655 1106 36-37 0.18838184632289057 1106 38-39 0.20765723106584488 1106 40-41 0.19285077708335052 1106 42-43 0.23934380200397243 1106 44-45 0.22148844853923322 1106 46-47 0.1973825759943182 1106 48-49 0.2964314007174593 1106 50-51 0.19425608076349477 1106 52-53 0.19309015478160063 1106 54-55 0.23721625715334227 1106 56-57 0.1571071789546039 1106 58-59 0.16686971732979572 1106 60-61 0.21148806295745715 1106 62-63 0.10096802903541402 1106 64-65 0.2034559893792789 1106 66-67 0.19176038333385748 1106 68-69 0.19125057777688426 1106 70-71 0.23154425538935897 1106 72-73 0.18437312569255937 1106 74-75 0.20176505556127466 1106 76-77 0.14937241378795818 1106 78-79 0.11021242350305016 1106 80-81 0.08694082956841243 1106 82-83 0.07726341235539991 1106 84-85 0.12485765760352763 1106 86-87 0.1229838498784801 1106 88-89 0.20051730605137408 1106 90-91 0.13551451084984478 1106 92-93 0.14815926801677648 1106 94-95 0.17917407560043586 1106 96-97 0.1631565411891458 1106 98-99 0.15597867658784992 1106 100 0.12850028883614328 1107 1 0.10732101572398278 1107 2 0.09282916717497613 1107 3 0.09430010410408585 1107 4 0.0828773263999878 1107 5 0.03354352250116932 1107 6 0.10973728094890589 1107 7 0.0976172598465439 1107 8 0.08084626448419385 1107 9 0.15349699851290666 1107 10-11 0.13237079364798632 1107 12-13 0.12962267100028413 1107 14-15 0.19700593710545178 1107 16-17 0.29022238817182 1107 18-19 0.2007867883924277 1107 20-21 0.1729619352401599 1107 22-23 0.19924942808719948 1107 24-25 0.15765912196020793 1107 26-27 0.14341094898028217 1107 28-29 0.14799650702991585 1107 30-31 0.17459148904539745 1107 32-33 0.18622490232790767 1107 34-35 0.16898102685760108 1107 36-37 0.12807299488985535 1107 38-39 0.1468209393488138 1107 40-41 0.14170924902715853 1107 42-43 0.18433135001394874 1107 44-45 0.23796643854704058 1107 46-47 0.21903163262696523 1107 48-49 0.21490606063084527 1107 50-51 0.17671307262283875 1107 52-53 0.17354918563040655 1107 54-55 0.15922620153062894 1107 56-57 0.17947576359753015 1107 58-59 0.1887171259386875 1107 60-61 0.11824580193620449 1107 62-63 0.06305535363641468 1107 64-65 0.10996277580947833 1107 66-67 0.14265014592356806 1107 68-69 0.05381914979567881 1107 70-71 0.03115993648069093 1107 72-73 0.06609409208939354 1107 74-75 0.10563339728519594 1107 76-77 0.11870235900684634 1107 78-79 0.020902150898194805 1107 80-81 0.09944475070197711 1107 82-83 0.10878746133705164 1107 84-85 0.10791047049086444 1107 86-87 0.0928619886980897 1107 88-89 0.19023987150561084 1107 90-91 0.06103071373885882 1107 92-93 0.07278479976073982 1107 94-95 0.06666714188863665 1107 96-97 0.0979163382848256 1107 98-99 0.09973049369853015 1107 100 0.13545475395526552 1108 1 0.08508864200090471 1108 2 0.017955568031901237 1108 3 0.058824237830236825 1108 4 0.052855426029758235 1108 5 -0.01563422909480039 1108 6 0.019369552096520692 1108 7 -0.008184230231108813 1108 8 0.02637739826733565 1108 9 0.10221028805785437 1108 10-11 0.12627640291332654 1108 12-13 0.059955401884252524 1108 14-15 0.10734840663511847 1108 16-17 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2214 90-91 -0.11004506483482146 2214 92-93 -0.045640511010020646 2214 94-95 -0.09009199608325957 2214 96-97 -0.11674825163148483 2214 98-99 -0.1195514568115712 2214 100 -0.3024561338317717 2215 1 -0.4978944655874429 2215 2 -0.4627258169269659 2215 3 -0.45283466483502366 2215 4 -0.32566311820762905 2215 5 -0.3155596623724719 2215 6 -0.2988738612624715 2215 7 -0.34386363933835185 2215 8 -0.4054094013135554 2215 9 -0.4822131642577432 2215 10-11 -0.47912051434472147 2215 12-13 -0.458230357108242 2215 14-15 -0.49400878120989233 2215 16-17 -0.4692890506371654 2215 18-19 -0.4983936020624924 2215 20-21 -0.5415505974629227 2215 22-23 -0.5251449640538581 2215 24-25 -0.5675516949709802 2215 26-27 -0.5936085239170481 2215 28-29 -0.5991187577577293 2215 30-31 -0.5187842799937954 2215 32-33 -0.5669296469066296 2215 34-35 -0.5686807577050033 2215 36-37 -0.6903490717826841 2215 38-39 -0.6408219542760207 2215 40-41 -0.6269519116399707 2215 42-43 -0.7150166258072304 2215 44-45 -0.7091815837201381 2215 46-47 -0.6780938392014022 2215 48-49 -0.658907657711012 2215 50-51 -0.7065355910658511 2215 52-53 -0.7406280180914422 2215 54-55 -0.7104303631988245 2215 56-57 -0.6965911392025674 2215 58-59 -0.7140661520801288 2215 60-61 -0.712068199077379 2215 62-63 -0.6637163295006445 2215 64-65 -0.5405894923492625 2215 66-67 -0.6288939696581224 2215 68-69 -0.665726680410593 2215 70-71 -0.5985406530352506 2215 72-73 -0.6375367065878024 2215 74-75 -0.6398789269252276 2215 76-77 -0.5891211201144131 2215 78-79 -0.5976225598330842 2215 80-81 -0.5876203769433985 2215 82-83 -0.5653564626845835 2215 84-85 -0.6154257126376876 2215 86-87 -0.5703252309791935 2215 88-89 -0.500551775495353 2215 90-91 -0.5147639221843008 2215 92-93 -0.47051847159580973 2215 94-95 -0.4811605189543471 2215 96-97 -0.5007656083481677 2215 98-99 -0.5390054434324298 2215 100 -0.6985870324034558 2216 1 -0.662939009986264 2216 2 -0.6571772542699605 2216 3 -0.6292481295093069 2216 4 -0.5185484382310221 2216 5 -0.41168204145458986 2216 6 -0.4452020741097087 2216 7 -0.45537755937245095 2216 8 -0.474434548360648 2216 9 -0.5898303863200027 2216 10-11 -0.6033552719958948 2216 12-13 -0.6001781745103543 2216 14-15 -0.6658379924274485 2216 16-17 -0.7280934074819001 2216 18-19 -0.6413398066231508 2216 20-21 -0.6392341418481635 2216 22-23 -0.6650165559085224 2216 24-25 -0.73003557523095 2216 26-27 -0.6760182640685812 2216 28-29 -0.7307238845029076 2216 30-31 -0.7347667986345172 2216 32-33 -0.8117435971210796 2216 34-35 -0.8207134509851528 2216 36-37 -0.8345931931243413 2216 38-39 -0.8440853365026015 2216 40-41 -0.8274748075239557 2216 42-43 -0.8570830557944618 2216 44-45 -0.8721483361173199 2216 46-47 -0.8292004110763713 2216 48-49 -0.802490836445422 2216 50-51 -0.7625728790088004 2216 52-53 -0.864792487427124 2216 54-55 -0.835344069258646 2216 56-57 -0.8299441087971502 2216 58-59 -0.8296380138062247 2216 60-61 -0.8413766007931756 2216 62-63 -0.8630903238749568 2216 64-65 -0.7420190451818343 2216 66-67 -0.8653906039544239 2216 68-69 -0.7763524882925026 2216 70-71 -0.7470669211413963 2216 72-73 -0.7401244148002988 2216 74-75 -0.6706513299909389 2216 76-77 -0.6986258184158345 2216 78-79 -0.6931996275770445 2216 80-81 -0.7300130673358822 2216 82-83 -0.7223558331047499 2216 84-85 -0.7176869583218135 2216 86-87 -0.645163708497563 2216 88-89 -0.5413748181510485 2216 90-91 -0.6537611967898229 2216 92-93 -0.6356770134351493 2216 94-95 -0.5813240466657845 2216 96-97 -0.6899938136730555 2216 98-99 -0.6178748455188909 2216 100 -0.8560189126164026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 24.0 8 108.0 9 237.0 10 249.0 11 146.0 12 148.0 13 191.0 14 355.0 15 646.0 16 1130.0 17 1985.0 18 3455.0 19 5198.0 20 7574.0 21 11029.0 22 15924.0 23 18667.0 24 18442.0 25 16777.0 26 16725.0 27 18775.0 28 23555.0 29 30077.0 30 37342.0 31 47449.0 32 60781.0 33 80231.0 34 115149.0 35 177818.0 36 315654.0 37 701676.0 38 971998.0 39 351768.0 40 1048.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0405189215966 20.117860478800413 13.706563643315281 24.135056956287702 2 14.626586944057662 21.11481693832419 40.16522237693505 24.093373740683102 3 17.712066142345215 26.79438565867473 31.32784637027631 24.16570182870375 4 12.16233092577205 18.204375566448856 39.16405669978907 30.469236807990026 5 12.394775312078389 38.74794497748895 35.80945117056834 13.047828539864327 6 29.168991487143565 38.541455412427794 18.739882321218712 13.54967077920993 7 25.250669078812226 33.816515967632604 23.3668956610538 17.56591929250137 8 24.99545428782696 35.39945097625517 21.251523837387015 18.353570898530858 9 26.293085513989144 15.452157711598119 20.928496942173048 37.326259832239685 10-11 23.854359176642376 26.366766906996652 29.136289609482063 20.642584306878906 12-13 25.47525957202559 24.21445308046438 28.683462349442983 21.62682499806705 14-15 22.992313087931812 25.16855151030475 26.93598761078009 24.903147790983347 16-17 21.479264710242585 28.123078818230262 28.160263642038903 22.237392829488254 18-19 21.329398848812726 27.64766029327788 29.980929304101426 21.042011553807964 20-21 22.217418959687596 27.045609680625265 29.314684370775247 21.42228698891189 22-23 21.976623119634432 26.739653484614383 28.585422555606666 22.69830084014452 24-25 21.874045811530333 27.014607192140303 29.07445847961493 22.036888516714434 26-27 22.25611988502533 26.66197516024263 29.064046106500147 22.017858848231896 28-29 21.80483643325824 26.73275711816408 29.58225055465788 21.8801558939198 30-31 22.095282682595055 26.9273813299226 29.321897908743754 21.65543807873859 32-33 21.851000344819397 27.239225212932123 28.926481392447883 21.983293049800604 34-35 21.95471064881931 27.198165728530576 29.127774234035712 21.719349388614397 36-37 22.509889577779855 26.62666334024554 28.990087734630222 21.873359347344383 38-39 22.743568885063734 26.361371014533585 29.15561628917649 21.739443811226188 40-41 22.27971771603633 26.38964537262109 29.544675331014986 21.785961580327598 42-43 22.366407061879244 26.54994607401816 29.022154208650957 22.061492655451634 44-45 22.23468998622685 27.13775375812989 28.74047117008855 21.887085085554705 46-47 22.19155714384936 26.95471855617279 28.328143858531774 22.52558044144608 48-49 22.674201461966078 26.833371118700217 28.23728314912421 22.25514427020949 50-51 22.589891925255838 26.978405363505676 27.81678074337916 22.614921967859328 52-53 22.075119228940864 27.34743495195951 27.821402139552166 22.756043679547467 54-55 22.23211998225611 27.27515612724493 27.819267192998893 22.673456697500065 56-57 22.504480600119482 27.08464415082861 27.785077312363963 22.625797936687945 58-59 22.021884070482322 27.123443986631113 28.22456030454297 22.630111638343596 60-61 22.40364589561542 27.119452847008606 27.920469388911805 22.55643186846417 62-63 22.495778629150358 26.89940392998651 27.844814086044543 22.760003354818586 64-65 21.941897928066474 26.875394372408117 28.140629515011213 23.042078184514196 66-67 22.37709829679091 26.709900773089906 28.0585080678584 22.854492862260784 68-69 22.330002298301142 26.80998586454701 27.92534174329172 22.93467009386013 70-71 21.979129978949782 26.688317538844615 27.99223517270188 23.34031730950372 72-73 22.208756690788313 26.578829465269333 27.94135449813456 23.27105934580779 74-75 22.223993229909034 26.858494647470543 27.79655719381957 23.12095492880085 76-77 22.13357202486341 26.646503042399637 27.85635326171714 23.363571671019816 78-79 21.935349756764236 26.7718075520786 27.934657488626147 23.358185202531022 80-81 21.713654245852958 26.990157573184252 28.100698621627785 23.195489559335005 82-83 21.852917346888965 26.619289449862745 28.044045970413894 23.4837472328344 84-85 22.00973130708887 26.65332378210823 27.593594170454637 23.743350740348262 86-87 21.69436352270985 26.841149652134828 27.947582373083925 23.5169044520714 88-89 22.059674373560938 26.23145188662308 28.011992142401287 23.6968815974147 90-91 21.777176197470062 26.23044486328645 28.12494778580698 23.867431153436506 92-93 21.645369544801767 26.58164854089311 27.88720876956436 23.885773144740764 94-95 21.136532136607816 26.59256259728227 28.183904814978987 24.087000451130926 96-97 21.423948485128946 26.23613658124264 27.812985030251554 24.526929903376853 98-99 21.282898217788308 26.422003380367332 27.837446200952648 24.45765220089171 100 21.294331635516713 26.610128149309947 27.599311781028867 24.496228434144474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 340.0 1 205.5 2 249.0 3 432.0 4 282.5 5 187.0 6 403.5 7 987.5 8 1828.5 9 2304.0 10 1789.0 11 1107.5 12 1096.0 13 1286.5 14 1491.5 15 1757.5 16 2071.0 17 2419.5 18 2849.0 19 3387.0 20 4166.0 21 5084.0 22 6256.0 23 7851.5 24 10064.5 25 13076.5 26 16979.5 27 21267.0 28 25689.0 29 30821.0 30 36757.0 31 42937.0 32 50182.0 33 56733.5 34 62257.0 35 67973.0 36 74918.5 37 83901.0 38 89146.0 39 89743.5 40 91072.5 41 92135.5 42 93055.0 43 98193.5 44 107942.0 45 116519.0 46 117950.0 47 121702.5 48 140605.0 49 142574.0 50 136202.5 51 131190.5 52 112989.5 53 103423.0 54 97768.5 55 88032.0 56 78812.5 57 69393.5 58 59599.0 59 52779.5 60 44416.5 61 34535.0 62 26054.0 63 19746.5 64 14285.0 65 9916.5 66 7261.5 67 5296.0 68 3971.5 69 3030.5 70 2321.5 71 1800.5 72 1451.5 73 1264.0 74 988.5 75 680.0 76 490.0 77 337.5 78 194.5 79 101.0 80 52.0 81 29.0 82 21.0 83 15.0 84 9.0 85 5.0 86 4.0 87 1.5 88 1.0 89 1.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025881850336070913 2 0.0026864705412124237 3 1.9657101521066518E-4 4 0.050027323371114284 5 0.0017691391368959864 6 0.0040952294835555235 7 3.2761835868444196E-5 8 1.6380917934222098E-4 9 3.2761835868444196E-5 10-11 3.2761835868444196E-5 12-13 0.0 14-15 3.2761835868444196E-5 16-17 3.767611124871082E-4 18-19 1.6380917934222098E-4 20-21 2.784756048817756E-4 22-23 0.0 24-25 0.0 26-27 2.457137690133314E-4 28-29 0.0 30-31 1.3104734347377679E-4 32-33 3.112374407502198E-4 34-35 9.828550760533259E-5 36-37 8.190458967111049E-5 38-39 7.535222249742164E-4 40-41 5.07808455960885E-4 42-43 0.0 44-45 5.241893738951071E-4 46-47 0.0 48-49 1.3104734347377679E-4 50-51 0.0 52-53 3.6038019455288616E-4 54-55 4.5866570215821875E-4 56-57 2.1295193314488725E-4 58-59 6.716176353031059E-4 60-61 7.535222249742164E-4 62-63 1.9657101521066518E-4 64-65 4.5866570215821875E-4 66-67 0.0011466642553955469 68-69 0.002539042279804425 70-71 0.003505516437923529 72-73 0.0030959934895679764 74-75 0.00273561329501509 76-77 0.00445560967810841 78-79 0.001523425367882655 80-81 0.001179426091263991 82-83 6.87998553237328E-4 84-85 6.060939635662175E-4 86-87 0.0 88-89 0.0 90-91 3.2761835868444196E-5 92-93 8.190458967111049E-5 94-95 9.828550760533259E-5 96-97 3.112374407502198E-4 98-99 3.2761835868444196E-5 100 0.07011032875847058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3052332.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.06919981255191 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13200721905736 43.32911954521993 2 15.238091662018405 18.306799456155296 3 5.756802399025541 10.374195407653303 4 2.723168406290262 6.543141884827132 5 1.485149861836941 4.460588190113354 6 0.8352129389905476 3.010234375095116 7 0.5174470540309997 2.1757841336703128 8 0.33932763800570565 1.6306511751428814 9 0.23189989470148326 1.2537037000199833 >10 0.7268538024694484 6.259638858632795 >50 0.00794772784736167 0.32736607132818113 >100 0.004873795887415024 0.6039351138160175 >500 7.305599031196396E-4 0.3155757900052044 >1k 2.4351996770654468E-4 0.3365426610964011 >5k 2.4351996770654468E-4 1.0727236372241122 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8025 0.26291373284426467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7653 0.2507263299012034 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 6322 0.2071203263603042 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4129 0.13527362030080606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.276183586844419E-5 0.0 3 0.0 0.0 0.0 3.276183586844419E-5 0.0 4 0.0 0.0 0.0 3.276183586844419E-5 0.0 5 0.0 0.0 0.0 3.276183586844419E-5 0.0 6 0.0 0.0 0.0 3.276183586844419E-5 0.0 7 0.0 0.0 0.0 3.276183586844419E-5 0.0 8 0.0 0.0 0.0 3.276183586844419E-5 0.0 9 0.0 0.0 0.0 1.3104734347377676E-4 0.0 10-11 0.0 0.0 0.0 1.6380917934222095E-4 0.0 12-13 0.0 0.0 0.0 1.6380917934222095E-4 0.0 14-15 0.0 0.0 0.0 2.2933285107910935E-4 0.0 16-17 0.0 0.0 0.0 2.2933285107910935E-4 0.0 18-19 0.0 0.0 0.0 2.784756048817756E-4 0.0 20-21 0.0 0.0 0.0 3.276183586844419E-4 0.0 22-23 0.0 0.0 0.0 5.078084559608849E-4 0.0 24-25 0.0 0.0 0.0 9.173314043164375E-4 0.0 26-27 0.0 0.0 0.0 0.0014579016961457666 0.0 28-29 0.0 0.0 0.0 0.004439228760174188 0.0 30-31 0.0 0.0 0.0 0.013186638937048788 0.0 32-33 0.0 0.0 0.0 0.03331878707820775 0.0 34-35 0.0 0.0 0.0 0.06704709710477104 0.0 36-37 0.0 0.0 0.0 0.12153003015399375 0.0 38-39 0.0 0.0 0.0 0.1996342468643647 0.0 40-41 0.0 0.0 0.0 0.2886972976727302 0.0 42-43 0.0 0.0 0.0 0.37458245040185667 0.0 44-45 0.0 0.0 0.0 0.4808946077949581 0.0 46-47 0.0 0.0 0.0 0.6143335652871312 1.6380917934222095E-5 48-49 0.0 0.0 0.0 0.7601073539837737 3.276183586844419E-5 50-51 0.0 0.0 0.0 0.913989697057856 3.276183586844419E-5 52-53 0.0 0.0 0.0 1.0736217423268504 3.276183586844419E-5 54-55 0.0 0.0 0.0 1.24262367265422 3.276183586844419E-5 56-57 0.0 0.0 0.0 1.4436339166250591 3.276183586844419E-5 58-59 3.276183586844419E-5 0.0 0.0 1.6759644756861312 3.276183586844419E-5 60-61 3.276183586844419E-5 0.0 0.0 1.9268382338487426 3.276183586844419E-5 62-63 3.276183586844419E-5 0.0 0.0 2.1821512207715283 3.276183586844419E-5 64-65 3.276183586844419E-5 0.0 0.0 2.4546641715252466 3.276183586844419E-5 66-67 4.914275380266628E-5 0.0 0.0 2.7586448656306066 3.276183586844419E-5 68-69 6.552367173688838E-5 0.0 0.0 3.088376362728563 3.276183586844419E-5 70-71 6.552367173688838E-5 0.0 0.0 3.4655306172460927 3.276183586844419E-5 72-73 6.552367173688838E-5 0.0 0.0 3.846042959940138 3.276183586844419E-5 74-75 6.552367173688838E-5 0.0 0.0 4.222361787643022 3.276183586844419E-5 76-77 6.552367173688838E-5 0.0 0.0 4.6276584591715455 3.276183586844419E-5 78-79 6.552367173688838E-5 0.0 0.0 5.073121141474781 3.276183586844419E-5 80-81 6.552367173688838E-5 0.0 0.0 5.565924676607918 3.276183586844419E-5 82-83 6.552367173688838E-5 0.0 0.0 6.077107601663252 3.276183586844419E-5 84-85 6.552367173688838E-5 0.0 0.0 6.596055081819409 3.276183586844419E-5 86-87 6.552367173688838E-5 0.0 0.0 7.144848594451718 3.276183586844419E-5 88 6.552367173688838E-5 0.0 0.0 7.567427134400845 3.276183586844419E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 9495 0.0 22.125662 12-13 CATGGGG 5940 0.0 19.23547 4 GTACTTT 10975 0.0 18.906445 14-15 TGATCGC 630 0.0 18.64988 9 AGTACTT 10285 0.0 18.621172 12-13 GTATAAG 1470 0.0 17.913862 1 ATATCGT 295 7.167E-8 17.52485 6 CGACGAG 610 0.0 17.335217 24-25 ATGGGGG 4125 0.0 17.318222 5 GTACTAG 1335 0.0 16.907465 1 ACTTTTT 12410 0.0 16.833858 16-17 GTGATCG 680 0.0 16.587423 8 TATTCGC 170 0.0019202465 16.587423 9 GGGCTAT 1965 0.0 16.503279 6 TAGGCAT 1900 0.0 16.078423 5 CTAACGC 470 1.8189894E-11 16.00682 3 TCTAACG 530 0.0 15.96933 2 CTCGTCG 860 0.0 15.848061 9 GTGTAGG 1785 0.0 15.806349 1 ACATGGG 16980 0.0 15.78411 3 >>END_MODULE