FastQCFastQC Report
Mon 16 Jan 2017
ERR1146603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314300
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.038.9152031
GTATCAA53400.036.1082531
ATCAACG53750.033.2377173
CAACGCA54150.032.9048775
AACGCAG61500.030.2600066
TATCAAC64250.029.269342
TCAACGC62850.028.6492334
ACGCAGA65150.027.2663067
CGCAGAG67900.025.8843738
GTACATG114300.025.38651
TACATGG112050.024.7971442
GTACTAG16250.023.7314551
ACATGGG114150.023.3524913
TAACGCC6450.023.3243624
TAGTACT19950.021.4446054
GCAGAGT81550.021.377639
CTAACGC5950.021.3340263
CATGGGG86650.020.9429684
CTAGTAC20950.020.6457123
CTGTGCG19150.020.6119989
Produced by FastQC (version 0.11.5)